| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,686,595 | G→A | 39.0% | intergenic (+570/+248) | sseA → / ← sseB | 3‑mercaptopyruvate sulfurtransferase/rhodanase‑like enzyme, sulfur transfer from thiosulfate |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,686,595 | 0 | G | A | 39.0% | 60.1 / 72.4 | 110 | intergenic (+570/+248) | sseA/sseB | 3‑mercaptopyruvate sulfurtransferase/rhodanase‑like enzyme, sulfur transfer from thiosulfate |
| Reads supporting (aligned to +/- strand): ref base G (32/35); new base A (32/11); total (64/46) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.14e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 110 total aligned reads displayed.
TATTATCCACTGCGGCCCTTTCCGCCGTCTCGCAAACGGGCGCTGGCTTTAGGAAAGGATGTTCCGTGGCCGTAAATGCAGGTGTTTCACAGCGCTTGCTATCGCGGCAATATCGCCAGTGGTGCTGTCGTG > minE/1686530‑1686661 | tattatCCACTGCGGCCCTTTCCGCCGTCTCGCAAACGGGCGCTGGCTTTAGGAAAGGATGTTCCGTgg > 1:3143464/1‑69 (MQ=255)tattatCCACTGCGGCCCTTTCCGCCGTCTCGCAAACGGGCGCTGGCTTTAGGAAAGGATGTTCCGTgg > 1:1519798/1‑69 (MQ=255)tattatCCACTGCGGCCCTTTCCGCCGTCTCGCAAACGGGCGCTGGCTTTAGGAAAGGATGTTCCGTgg > 1:2103023/1‑69 (MQ=255)tattatCCACTGCGGCCCTTTCCGCCGTCTCGCAAACGGGCGCTGGCTTTAGGAAAGGATGTTCCGTgg > 1:2573279/1‑69 (MQ=255)tattatCCACTGCGGCCCTTTCCGCCGTCTCGCAAACGGGCGCTGGCTTTAGGAAAGGATGTTCCGTgg > 1:2775626/1‑69 (MQ=255)tattatCCACTGCGGCCCTTTCCGCCGTCTCGCAAACGGGCGCTGGCTTTAGGAAAGGATGTTCCGTgg > 1:772799/1‑69 (MQ=255)tattatCCACTGCGGCCCTTTCCGCCGTCTCGCAAACGGGCGCTGGCTTTAGGAAAGGATGTTCCGTgg > 1:831963/1‑69 (MQ=255)tattatCCACTGCGGCCCTTTCCGCCGTCTCGCAAACGGGCGCTGGCTTTAGGAAAGGATGTTCCGTgg > 1:1111751/1‑69 (MQ=255) ttatCCACTGCGGCCCTTTCCGCCGTCTCGCAAACGGGCGCTGGCTTTAGGAAAGGATGTTCCGTGGc > 1:455555/1‑68 (MQ=255) ttatCCACTGCGGCCCTTTCCGCCGTCTCGCAAACGGGCGCTGGCTTTAGGAAAGGATGTTACGTGGc > 1:1041371/1‑68 (MQ=255) cGGCCCTTTCCGCCGTCTCGCAAACGGGCGCTGGCTTTAGGAAAGGATGTTCCGTGGCCGTAAATGc > 1:669705/1‑67 (MQ=255) cGGCCCTTTCCGCCGTCTCGCAAACGGGCGCTGGCTTTAGGAAAGGATGTTCCGTGGCCGTAAATGCa > 1:443962/1‑68 (MQ=255) cGGCCCTTTCCGCCGTCTCGCAAACGGGCGCTGGCTTTAGGAAAGGATGTTCCGTGGCCGTAAATGCa > 1:421254/1‑68 (MQ=255) cGGCCCTTTCCGCCGTCTCGCAAACGGGCGCTGGCTTTAGGAAAGGATGTTCCGTGGCCGTAAATGCa > 1:2737942/1‑68 (MQ=255) cGGCCCTTTCCGCCGTCTCGCAAACGGGCGCTGGCTTTAGGAAAGGATGTTCCGTGGCCGTAAATGCa > 1:3077918/1‑68 (MQ=255) cGGCCCTTTCCGCCGTCTCGCAAACGGGCGCTGGCTTTAGGAAAGGATGTTCCGTGGCCGTAAATGCa > 1:2471740/1‑68 (MQ=255) cGGCCCTTTCCGCCGTCTCGCAAACGGGCGCTGGCTTTAGGAAAGGATGTTCCGTGGCCGTAAATGCa > 1:2336251/1‑68 (MQ=255) cGGCCCTTTCCGCCGTCTCGCAAACGGGCGCTGGCTTTAGGAAAGGATGTTCCGTGGCCGTAAATGCa > 1:924199/1‑68 (MQ=255) cGGCCCTTTCCGCCGTCTCGCAAACGGGCGCTGGCTTTAGGAAAGGATGTTCCGTGGCCGTAAATGCa > 1:2015104/1‑68 (MQ=255) cGGCCCTTTCCGCCGTCTCGCAAACGGGCGCTGGCTTTAGGAAAGGATGTTCCGTGGCCGTAAATGCa > 1:1636483/1‑68 (MQ=255) gCCCTTTCCGCCGTCTCGCAAACGGGCGCTGGCTTTAGGAAAGGATGTTCCGTGGCCGTAAATGCAGgt < 1:3235327/69‑1 (MQ=255) gCCCTTTCCGCCGTCTCGCAAACGGGCGCTGGCTTTAGGAAAGGATGTTCCGTGGCCGTAAATGCAGgt < 1:3228337/69‑1 (MQ=255) gCCCTTTCCGCCGTCTCGCAAACGGGCGCTGGCTTTAGGAAAGGATGTTCCGTGGCCGTAAATGCAGgt < 1:858722/69‑1 (MQ=255) gCCCTTTCCGCCGTCTCGCAAACGGGCGCTGGCTTTAGGAAAGGATGTTCCGTGGCCGTAAATGCAGgt < 1:3432174/69‑1 (MQ=255) gCCCTTTCCGCCGTCTCGCAAACGGGCGCTGGCTTTAGGAAAGGATGTTCCGTGGCCGTAAATGCAGgt < 1:2793721/69‑1 (MQ=255) gCCCTTTCCGCCGTCTCGCAAACGGGCGCTGGCTTTAGGAAAGGATGTTCCGTGGCCGTAAATGCAGgt < 1:2785577/69‑1 (MQ=255) gCCCTTTCCGCCGTCTCGCAAACGGGCGCTGGCTTTAGGAAAGGATGTTCCGTGGCCGTAAATGCAGgt < 1:513838/69‑1 (MQ=255) gCCCTTTCCGCCGTCTCGCAAACGGGCGCTGGCTTTAGGAAAGGATGTTCCGTGGCCGTAAATGCAGgt < 1:1200982/69‑1 (MQ=255) gggCGCTGGCTTAAGGAAAGGATGTTCCATGGCCGTAAATGCAGGTGTTTCACAGCGCTTGCTATcgcg < 1:2000935/69‑1 (MQ=255) gggCGCTGGCTTAAGGAAAGGATGTTCCATGGCCGTAAATGCAGGTGTTTCACAGCGCTTGCTATcgcg < 1:1400434/69‑1 (MQ=255) gggCGCTGGCTTAAGGAAAGGATGTTCCATGGCCGTAAATGCAGGTGTTTCACAGCGCTTGCTATcgcg < 1:149546/69‑1 (MQ=255) gggCGCTGGCTTAAGGAAAGGATGTTCCATGGCCGTAAATGCAGGTGTTTCACAGCGCTTGCTATcgcg < 1:118556/69‑1 (MQ=255) gggCGCTGGCTTAAGGAAAGGATGTTCCATGGCCGTAAATGCAGGTGTTTCACAGCGCTTGCTATcgcg < 1:756486/69‑1 (MQ=255) gggCGCTGGCTTAAGGAAAGGATGTTCCATGGCCGTAAATGCAGGTGTTTCACAGCGCTTGCTATcgcg < 1:1141136/69‑1 (MQ=255) gggCGCTGGCTTAAGGAAAGGATGTTCCATGGCCGTAAATGCAGGTGTTTCACAGCGCTTGCTATcgcg < 1:782447/69‑1 (MQ=255) gggCGCTGGCTTAAGGAAAGGATGTTCCATGGCCGTAAATGCAGGTGTTTCACAGCGCTTGCTATcgcg < 1:2755511/69‑1 (MQ=255) gggCGCTGGCTTAAGGAAAGGATGTTCCATGGCCGTAAATGCAGGTGTTTCACAGCGCTTGCTATcgcg < 1:2650765/69‑1 (MQ=255) gcgcTGGCTTTAGGAAAGGATGTTCCGTGGCCGTAAATGCAGGTGTTTCACAGCGCTTGCTATCGCGGc < 1:3363755/69‑1 (MQ=255) gcgcTGGCTTTAGGAAAGGATGTTCCGTGGCCGTAAATGCAGGTGTTTCACAGCGCTTGCTATCGCGGc < 1:3021439/69‑1 (MQ=255) gcgcTGGCTTTAGGAAAGGATGTTCCGTGGCCGTAAATGCAGGTGTTTCACAGCGCTTGCTATCGCGGc < 1:241162/69‑1 (MQ=255) gcgcTGGCTTTAGGAAAGGATGTTCCGTGGCCGTAAATGCAGGTGTTTCACAGCGCTTGCTATCGCGGc < 1:326888/69‑1 (MQ=255) gcgcTGGCTTTAGGAAAGGATGTTCCGTGGCCGTAAATGCAGGTGTTTCACAGCGCTTGCTATCGCGGc < 1:3306383/69‑1 (MQ=255) gcgcTGGCTTTAGGAAAGGATGTTCCGTGGCCGTAAATGCAGGTGTTTCACAGCGCTTGCTATCGCGGc < 1:3334910/69‑1 (MQ=255) gcgcTGGCTTTAGGAAAGGATGTTCCGTGGCCGTAAATGCAGGTGTTTCACAGCGCTTGCTATCGCGGc < 1:639010/69‑1 (MQ=255) gcgcTGGCTTTAGGAAAGGATGTTCCGTGGCCGTAAATGCAGGTGTTTCACAGCGCTTGCTATCGCGGc < 1:1093223/69‑1 (MQ=255) gcgcTGGCTTTAGGAAAGGATGTTCCGTGGCCGTAAATGCAGGTGTTTCACAGCGCTTGCTACCGCGGc < 1:437506/69‑1 (MQ=255) gcTGGCTTTAGGAAAGGATGTTCCGTGGCCGTAAATGCAGGTGTTTCACAGCGCTTGCTATCGCGGCaa < 1:1514847/69‑1 (MQ=255) gAAAGGATGTTCCGTGGCCGTAAATGCAGGTGTTTCACAGCGCTTGCTATCGCGGCAATATCGCCAgt < 1:1372925/68‑1 (MQ=255) gAAAGGATGTTCCGTGGCCGTAAATGCAGGTGTTTCACAGCGCTTGCTATCGCGACAATATCGCCAgt < 1:1317994/68‑1 (MQ=255) aaaGGATGTTCCGTGGCCGTAAATGCAGGTGTTTCACAGCGCGTGCTATCGCGGCAATATCGCCAgt < 1:3190136/67‑1 (MQ=255) aaaGGATGTTCCATGGCCGTAAATGCAGGTGTTTcaca > 1:1740970/1‑38 (MQ=255) aaaGGATGTTCCATGGCCGTAAATGCAGGTGTTTcaca > 1:2007351/1‑38 (MQ=255) aaaGGATGTTCCATGGCCGTAAATGCAGGTGTTTcaca > 1:1345691/1‑38 (MQ=255) aaaGGATGTTCCATGGCCGTAAATGCAGGTGTTTcaca > 1:1979194/1‑38 (MQ=255) aaaGGATGTTCCATGGCCGTAAATGCAGGTGTTTcaca > 1:519062/1‑38 (MQ=255) aaaGGATGTTCCATGGCCGTAAATGCAGGTGTTTcaca > 1:2077256/1‑38 (MQ=255) aaaGGATGTTCCATGGCCGTAAATGCAGGTGTTTcaca > 1:558031/1‑38 (MQ=255) aaaGGATGTTCCATGGCCGTAAATGCAGGTGTTTcaca > 1:1837287/1‑38 (MQ=255) aaaGGATGTTCCATGGCCGTAAATGCAGGTGTTTcaca > 1:2225926/1‑38 (MQ=255) aaaGGATGTTCCATGGCCGTAAATGCAGGTGTTTcaca > 1:2288466/1‑38 (MQ=255) aaaGGATGTTCCATGGCCGTAAATGCAGGTGTTTcaca > 1:1828406/1‑38 (MQ=255) aaaGGATGTTCCATGGCCGTAAATGCAGGTGTTTcaca > 1:1766945/1‑38 (MQ=255) aaaGGATGTTCCATGGCCGTAAATGCAGGTGTTTcaca > 1:2377379/1‑38 (MQ=255) aaaGGATGTTCCATGGCCGTAAATGCAGGTGTTTcaca > 1:2403534/1‑38 (MQ=255) aaaGGATGTTCCATGGCCGTAAATGCAGGTGTTTcaca > 1:1127802/1‑38 (MQ=255) aaaGGATGTTCCATGGCCGTAAATGCAGGTGTTTcaca > 1:3151518/1‑38 (MQ=255) aaaGGATGTTCCATGGCCGTAAATGCAGGTGTTTcaca > 1:1586384/1‑38 (MQ=255) aaaGGATGTTCCATGGCCGTAAATGCAGGTGTTTcaca > 1:3131543/1‑38 (MQ=255) aaaGGATGTTCCATGGCCGTAAATGCAGGTGTTTcaca > 1:854044/1‑38 (MQ=255) aaaGGATGTTCCATGGCCGTAAATGCAGGTGTTTcaca > 1:1478564/1‑38 (MQ=255) aaaGGATGTTCCATGGCCGTAAATGCAGGTGTTTcaca > 1:282306/1‑38 (MQ=255) aaaGGATGTTCCATGGCCGTAAATGCAGGTGTTTcaca > 1:1723445/1‑38 (MQ=255) aaaGGATGTTCCATGGCCGTAAATGCAGGTGTTTcaca > 1:886611/1‑38 (MQ=255) aaaGGATGTTCCATGGCCGTAAATGCAGGTGTTTcaca > 1:1699707/1‑38 (MQ=255) aaaGGATGTTCCATGGCCGTAAATGCAGGTGTTTcaca > 1:1410006/1‑38 (MQ=255) aaaGGATGTTCCATGGCCGTAAATGCAGGTGTTTcaca > 1:1475056/1‑38 (MQ=255) aaaGGATGTTCCATGGCCGTAAATGCAGGTGTTTcaca > 1:925021/1‑38 (MQ=255) aaaGGATGTTCCATGGCCGTAAATGCAGGTGTTTcaca > 1:948381/1‑38 (MQ=255) aaaGGATGTTCCATGGCCGTAAATGCAGGTGTTTc > 1:2721916/1‑35 (MQ=255) gATGTTCCGTGGCAGTAAATGCAGGTGTTTCACAGCGCTTGCTATCGCGGCAATATCGCCAGTGGTGCt < 1:2555595/69‑1 (MQ=255) aTGTTCCGTGGCCGTAAATGCAGGTGTTTCACAGCGCTTGCTATCGCGGc < 1:100427/50‑1 (MQ=255) aTGTTCCGTGGCCGTAAATGCAGGTGTTTCACAGCGCTTGCTATCGCGGc < 1:2794104/50‑1 (MQ=255) aTGTTCCGTGGCCGTAAATGCAGGTGTTTCACAGCGCTTGCTATCGCGGc < 1:1852495/50‑1 (MQ=255) aTGTTCCGTGGCCGTAAATGCAGGTGTTTCACAGCGCTTGCTATCGCGGc < 1:2463291/50‑1 (MQ=255) aTGTTCCGTGGCCGTAAATGCAGGTGTTTCACAGCGCTTGCTATCGCGGc < 1:754980/50‑1 (MQ=255) aTGTTCCGTGGCCGTAAATGCAGGTGTTTCACAGCGCTTGCTATCGCGGc < 1:330515/50‑1 (MQ=255) aTGTTCCGTGGCCGTAAATGCAGGTGTTTCACAGCGCTTGCTATCGCGGc < 1:847347/50‑1 (MQ=255) aTGTTCCGTGGCCGTAAATGCAGGTGTTTCACAGCGCTTGCTATCGCGGc < 1:3158841/50‑1 (MQ=255) ccGTGGCCGTAAATGCAGGTGTTTCACAGCGCTTGCTATCGCGGCAATATCGCCAGTGGTGCTGTCGt > 1:2609570/1‑68 (MQ=255) ccGTGGCCGTAAATGCAGGTGTTTCACAGCGCTTGCTATCGCGGCAATATCGCCAGTGGTGCTGTCGt > 1:1513205/1‑68 (MQ=255) ccGTGGCCGTAAATGCAGGTGTTTCACAGCGCTTGCTATCGCGGCAATATCGCCAGTGGTGCTGTCGt > 1:1481704/1‑68 (MQ=255) ccGTGGCCGTAAATGCAGGTGTTTCACAGCGCTTGCTATCGCGGCAATATCGCCAGTGGTGCTGTCGt < 1:2093352/68‑1 (MQ=255) ccGTGGCCGTAAATGCAGGTGTTTCACAGCGCTTGCTATCGCGGCAATATCGCCAGTGGTGCTGTCGt > 1:402644/1‑68 (MQ=255) ccGTGGCCGTAAATGCAGGTGTTTCACAGCGCTTGCTATCGCGGCAATATCGCCAGTGGTGCTGTCGTg > 1:3152658/1‑69 (MQ=255) ccGTGGCCGTAAATGCAGGTGTTTCACAGCGCTTGCTATCGCGGCAATATCGCCAGTGGTGCTGTCGTg > 1:33495/1‑69 (MQ=255) ccGTGGCCGTAAATGCAGGTGTTTCACAGCGCTTGCTATCGCGGCAATATCGCCAGTGGTGCTGTCGTg > 1:580670/1‑69 (MQ=255) ccGTGGCCGTAAATGCAGGTGTTTCACAGCGCTTGCTATCGCGGCAATATCGCCAGTGGTGCTGTCGTg > 1:2545595/1‑69 (MQ=255) ccGTGGCCGTAAATGCAGGTGTTTCACAGCGCTTGCTATCGCGGCAATATCGCCAGTGGTGCTGTCGTg > 1:896616/1‑69 (MQ=255) ccGTGGCCGTAAATGCAGGTGTTTCACAGCGCTTGCTATCGCGGCAATATCGCCAGTGGTGCTGTCGTg > 1:357936/1‑69 (MQ=255) ccGTGGCCGTAAATGCAGGTGTTTCACAGCGCTTGCTATCGCGGCAATATCGCCAGTGGTGCTGTCGTg > 1:928735/1‑69 (MQ=255) | TATTATCCACTGCGGCCCTTTCCGCCGTCTCGCAAACGGGCGCTGGCTTTAGGAAAGGATGTTCCGTGGCCGTAAATGCAGGTGTTTCACAGCGCTTGCTATCGCGGCAATATCGCCAGTGGTGCTGTCGTG > minE/1686530‑1686661 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |