Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	1,688,613	A→G	67.6%	Y144Y (TAT→TAC)	pepB ←	aminopeptidase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,688,613	0	A	G	67.6%	70.4 / 41.3	83	Y144Y (TAT→TAC)	pepB	aminopeptidase B
Reads supporting (aligned to +/- strand):  ref base A (25/2);  new base G (39/17);  total (64/19)
Fisher's exact test for biased strand distribution p-value = 2.50e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.01e-01

AGCCCCATATAACCTTGCTCACGCAGATCTTCGCCTTTGGTGATCCGATAAGTCACACGATCGCCCGCGACGTTGCTGATCAGATCAACAGCACGCTGTGCCAGTTGCGATGG  >  minE/1688566‑1688678                                                |                                                                  aGCCCCATATAACCTTGCTCACGCAGATCTTCGCCTTTGGTGATCCGGTAAg                                                               >  1:2062417/1‑52 (MQ=255) aGCCCCATATAACCTTGCTCACGCAGATCTTCGCCTTTGGTGATCCGGTAAGTCACACGa                                                       >  1:54626/1‑60 (MQ=255) aGCCCCATATAACCTTGCTCACGCAGATCTTCGCCTTTGGTGATCCGGTAAGTCACACGCTCCCCtgcg                                              >  1:611405/1‑69 (MQ=255) aGCCCCATATAACCTTGCTCACGCAGATCTTCGCCTTTGGTGATCCGGTAAGTCACACGATCCCCtgcg                                              >  1:266721/1‑69 (MQ=255) aGCCCCATATAACCTTGCTCACGCAGATCTTCGCCTTTGGTGATCCGGTAAGTCACACGATCCCCtgcg                                              >  1:1790002/1‑69 (MQ=255) aGCCCCATATAACCTTGCTCACGCAGATCTTCGCCTTTGGTGATCCGGTAAGTCACACGATCCCCtgcg                                              >  1:1858944/1‑69 (MQ=255) aGCCCCATATAACCTTGCTCACGCAGATCTTCGCCTTTGGTGATCCGGTAAGTCACACGATCCCCtgcg                                              >  1:1897189/1‑69 (MQ=255) aGCCCCATATAACCTTGCTCACGCAGATCTTCGCCTTTGGTGATCCGGTAAGTCACACGATCCCCtgcg                                              >  1:1924755/1‑69 (MQ=255) aGCCCCATATAACCTTGCTCACGCAGATCTTCGCCTTTGGTGATCCGGTAAGTCACACGATCCCCtgcg                                              >  1:2101051/1‑69 (MQ=255) aGCCCCATATAACCTTGCTCACGCAGATCTTCGCCTTTGGTGATCCGGTAAGTCACACGATCCCCtgcg                                              >  1:242345/1‑69 (MQ=255) aGCCCCATATAACCTTGCTCACGCAGATCTTCGCCTTTGGTGATCCGGTAAGTCACACGATCCCCtgcg                                              >  1:2588010/1‑69 (MQ=255) aGCCCCATATAACCTTGCTCACGCAGATCTTCGCCTTTGGTGATCCGGTAAGTCACACGATCCCCtgcg                                              >  1:2598468/1‑69 (MQ=255) aGCCCCATATAACCTTGCTCACGCAGATCTTCGCCTTTGGTGATCCGGTAAGTCACACGATCCCCtgcg                                              >  1:2811747/1‑69 (MQ=255) aGCCCCATATAACCTTGCTCACGCAGATCTTCGCCTTTGGTGATCCGGTAAGTCACACGATCCCCtgcg                                              >  1:2908098/1‑69 (MQ=255) aGCCCCATATAACCTTGCTCACGCAGATCTTCGCCTTTGGTGATCCGGTAAGTCACACGATCCCCtgcg                                              >  1:331165/1‑69 (MQ=255) aGCCCCATATAACCTTGCTCACGCAGATCTTCGCCTTTGGTGATCCGGTAAGTCACACGATCCCCtgcg                                              >  1:420325/1‑69 (MQ=255) aGCCCCATATAACCTTGCTCACGCAGATCTTCGCCTTTGGTGATCCGGTAAGTCACACGATCCCCtgcg                                              >  1:663799/1‑69 (MQ=255) aGCCCCATATAACCTTGCTCACGCAGATCTTCGCCTTTGGTGATCCGGTAAGTCACACGATCCCCtgcg                                              >  1:1049483/1‑69 (MQ=255) aGCCCCATATAACCTTGCTCACGCAGATCTTCGCCTTTGGTGATCCGGTAAGTCACACGATCCCCtgcg                                              >  1:1452832/1‑69 (MQ=255) aGCCCCATATAACCTTGCTCACGCAGATCTTCGCCTTTGGTGATCCGGTAAGTCACACGATCCCCtgcg                                              >  1:158618/1‑69 (MQ=255) aGCCCCATATAACCTTGCTCACGCAGATCTTCGCCTTTGGTGATCCGGTAAGTCACACGATCCCCtgcg                                              >  1:908042/1‑69 (MQ=255) aGCCCCATATAACCTTGCTCACGCAGATCTTCGCCTTTGGTGATCCGGTAAGTCACACGATCCCCtgcg                                              >  1:1661206/1‑69 (MQ=255)          taACCTTGCTCACGCAGATCTTCGCCTTTGGTGATCCGATa                                                                 >  1:1834619/1‑41 (MQ=255)          taACCTTGCTCACGCAGATCTTCGCCTTTGGTGATCCGATAAGTCACACGATCGcc                                                  >  1:2027953/1‑56 (MQ=255)          taACCTTGCTCACGCAGATCTTCGCCTTTGGTGATCCGATAAGTCACACGATCGcc                                                  >  1:1133760/1‑56 (MQ=255)          taACCTTGCTCACGCAGATCTTCGCCTTTGGTGATCCGATAAGTCACACGATCGcc                                                  >  1:3401852/1‑56 (MQ=255)          taACCTTGCTCACGCAGATCTTCGCCTTTGGTGATCCGATAAGTCACACGATCGcc                                                  >  1:3370930/1‑56 (MQ=255)          taACCTTGCTCACGCAGATCTTCGCCTTTGGTGATCCGATAAGTCACACGATCGcc                                                  >  1:3074077/1‑56 (MQ=255)          taACCTTGCTCACGCAGATCTTCGCCTTTGGTGATCCGATAAGTCACACGATCGcc                                                  >  1:2344048/1‑56 (MQ=255)          taACCTTGCTCACGCAGATCTTCGCCTTTGGTGATCCGATAAGTCACACGATCGcc                                                  >  1:2830676/1‑56 (MQ=255)          taACCTTGCTCACGCAGATCTTCGCCTTTGGTGATCCGATAAGTCACACGATCGcc                                                  >  1:2828828/1‑56 (MQ=255)          taACCTTGCTCACGCAGATCTTCGCCTTTGGTGATCCGATAAGTCACACGATCGCCCGCGACGTTGCt                                      >  1:104864/1‑68 (MQ=255)          taACCTTGCTCACGCAGATCTTCGCCTTTGGTGATCCGATAAGTCACACGATCGCCCGCGACGTTGCt                                      >  1:1711525/1‑68 (MQ=255)          taACCTTGCTCACGCAGATCTTCGCCTTTGGTGATCCGATAAGTCACACGATCGCCCGCGACGTTGCTg                                     >  1:1694078/1‑69 (MQ=255)          taACCTTGCTCACGCAGATCTTCGCCTTTGGTGATCCGATAAGTCACACGATCGCCCGCGACGTTGCTg                                     >  1:3038981/1‑69 (MQ=255)          taACCTTGCTCACGCAGATCTTCGCCTTTGGTGATCCGATAAGTCACACGATCGCCCGCGACGTTGCTg                                     >  1:37542/1‑69 (MQ=255)          taACCTTGCTCACGCAGATCTTCGCCTTTGGTGATCCGATAAGTCACACGATCGCCCGCGACGTTGCTg                                     >  1:2872284/1‑69 (MQ=255)          taACCTTGCTCACGCAGATCTTCGCCTTTGGTGATCCGATAAGTCACACGATCGCCCGCGACGTTGCTg                                     >  1:3229128/1‑69 (MQ=255)          taACCTTGCTCACGCAGATCTTCGCCTTTGGTGATCCGATAAGTCACACGATCGCCCGCGACGTTGCTg                                     >  1:2421840/1‑69 (MQ=255)          taACCTTGCTCACGCAGATCTTCGCCTTTGGTGATCCGATAAGTCACACGATCGCCCGCGACGTTGCTg                                     >  1:761091/1‑69 (MQ=255)          taACCTTGCTCACGCAGATCTTCGCCTTTGGTGATCCGATAAGTCACACGATCGCCCGCGACGTTGCTg                                     >  1:910315/1‑69 (MQ=255)          taACCTTGCTCACGCAGATCTTCGCCTTTGGTGATCCGATAAGTCACACGATCGCCCGCGACGTTGCTg                                     >  1:3272333/1‑69 (MQ=255)          taACCTTGCTCACGCAGATCTTCGCCTTTGGTGATCCGATAAGTCACACGATCGCCCGCGACGTTGCTg                                     >  1:814951/1‑69 (MQ=255)          taACCTTGCTCACGCAGATCTTCGCCTTTGGTGATCCGATAAGTCACACGATCGCCCGCGACGTTGCTg                                     >  1:1485933/1‑69 (MQ=255)          taACCTTGCTCACGCAGATCTTCGCCTTTGGTGATCCGATAAGTCACACGATCGCCCGCGACGTTGCTg                                     >  1:2666675/1‑69 (MQ=255)          taACCTTGCTCACGCAGATCTTCGCCTTTGGTGATCCGATAAGTCACACGATCGCCCGCGACGTTGCTg                                     >  1:2616994/1‑69 (MQ=255)          taACCTTGCTCACGCAGATCTTCGCCTTTGGTGATCCGATAAGTCACACGATCGCACGCGAc                                            >  1:1483720/1‑62 (MQ=255)               ttGCTCACGCAGATCTTCGCCTTTGGTGATCCGATAAGTCACACGATCGCCCGCGACGTTGCTgatcag                                <  1:419171/69‑1 (MQ=255)               ttGCTCACGCAGATCTTCGCCTTTGGTGATCCGATAAGTCACACGATCGCCCGCGACGTTGCTgatcag                                <  1:482291/69‑1 (MQ=255)                tGCTCACGCAGATCTTCGCCTTTTGTGATCCGGTAAGTCACACGATCCCCTGCGACGTTGCTgatcaga                               <  1:1963403/69‑1 (MQ=255)                tGCTCACGCAGATCTTCGCCTTTGGTGATCCGGTAAGTCACACGATCCCCTGCGACGTTGCTgatcaga                               <  1:344224/69‑1 (MQ=255)                tGCTCACGCAGATCTTCGCCTTTGGTGATCCGGTAAGTCACACGATCCCCTGCGACGTTGCTgatcaga                               <  1:576533/69‑1 (MQ=255)                tGCTCACGCAGATCTTCGCCTTTGGTGATCCGGTAAGTCACACGATCCCCTGCGACGTTGCTgatcaga                               <  1:74222/69‑1 (MQ=255)                tGCTCACGCAGATCTTCGCCTTTGGTGATCCGGTAAGTCACACGATCCCCTGCGACGTTGCTgatcaga                               <  1:1104258/69‑1 (MQ=255)                tGCTCACGCAGATCTTCGCCTTTGGTGATCCGGTAAGTCACACGATCCCCTGCGACGTTGCTgatcaga                               <  1:3131736/69‑1 (MQ=255)                tGCTCACGCAGATCTTCGCCTTTGGTGATCCGGTAAGTCACACGATCCCCTGCGACGTTGCTgatcaga                               <  1:607960/69‑1 (MQ=255)                tGCTCACGCAGATCTTCGCCTTTGGTGATCCGGTAAGTCACACGATCCCCTGCGACGTTGCTgatcaga                               <  1:1069425/69‑1 (MQ=255)                tGCTCACGCAGATCTTCGCCTTTGGTGATCCGGTAAGTCACACGATCCCCTGCGACGTTGCTgatcaga                               <  1:1717950/69‑1 (MQ=255)                tGCTCACGCAGATCTTCGCCTTTGGTGATCCGGTAAGTCACACGATCCCCTGCGACGTTGCTgatcaga                               <  1:2063032/69‑1 (MQ=255)                tGCTCACGCAGATCTTCGCCTTTGGTGATCCGGTAAGTCACACGATCCCCTGCGACGTTGCTgatcaga                               <  1:1167251/69‑1 (MQ=255)                tGCTCACGCAGATCTTCGCCTTTGGTGATCCGGTAAGTCACACGATCCCCTGCGACGTTGCTgatcaga                               <  1:3224745/69‑1 (MQ=255)                tGCTCACGCAGATCTTCGCCTTTGGTGATCCGGTAAGTCACACGATCCCCTGCGACGTTGCTgatcaga                               <  1:2520014/69‑1 (MQ=255)                tGCTCACGCAGATCTTCGCCTTTGGTGATCCGGTAAGTCACACGATCCCCTGCGACGTTGCTgatcaga                               <  1:3118559/69‑1 (MQ=255)                tGCTCACGCAGATCTTCGCCTTTGGTGATCCGGTAAGTCACACGATCCCCTGCGACGTTGCTgatcaga                               <  1:2977096/69‑1 (MQ=255)                tGCTCACGCAGATCTTCGCCTTTGGTGATCCGGTAAGTCACACGATCCCCTGCGACGTTGCTgatcaga                               <  1:2391797/69‑1 (MQ=255)                tGCTCACGCAGATCTTCGCCTTTGGTGATCCGGTAAGTCACACGATCCCCTGCGACGTTGCTgatcaga                               <  1:2552676/69‑1 (MQ=255)                                             ccGGTAAGTCACACGCTCCCCTGCGACGTTGCTGATCAGATCAACAGCACGCTGTGcc             >  1:900190/1‑58 (MQ=255)                                             ccGGTAAGTCACACGATCCCCTGCGACGTTGCTGATCAGATCAACAGCACGCTGTGCCAGTTGCGATgg  >  1:752018/1‑69 (MQ=255)                                             ccGGTAAGTCACACGATCCCCTGCGACGTTGCTGATCAGATCAACAGCACGCTGTGCCAGTTGCGATgg  >  1:882511/1‑69 (MQ=255)                                             ccGGTAAGTCACACGATCCCCTGCGACGTTGCTGATCAGATCAACAGCACGCTGTGCCAGTTGCGATgg  >  1:664227/1‑69 (MQ=255)                                             ccGGTAAGTCACACGATCCCCTGCGACGTTGCTGATCAGATCAACAGCACGCTGTGCCAGTTGCGATgg  >  1:765649/1‑69 (MQ=255)                                             ccGGTAAGTCACACGATCCCCTGCGACGTTGCTGATCAGATCAACAGCACGCTGTGCCAGTTGCGATgg  >  1:917770/1‑69 (MQ=255)                                             ccGGTAAGTCACACGATCCCCTGCGACGTTGCTGATCAGATCAACAGCACGCTGTGCCAGTTGCGATgg  >  1:599873/1‑69 (MQ=255)                                             ccGGTAAGTCACACGATCCCCTGCGACGTTGCTGATCAGATCAACAGCACGCTGTGCCAGTTGCGATgg  >  1:493452/1‑69 (MQ=255)                                             ccGGTAAGTCACACGATCCCCTGCGACGTTGCTGATCAGATCAACAGCACGCTGTGCCAGTTGCGATgg  >  1:391445/1‑69 (MQ=255)                                             ccGGTAAGTCACACGATCCCCTGCGACGTTGCTGATCAGATCAACAGCACGCTGTGCCAGTTGCGATgg  >  1:2812165/1‑69 (MQ=255)                                             ccGGTAAGTCACACGATCCCCTGCGACGTTGCTGATCAGATCAACAGCACGCTGTGCCAGTTGCGATgg  >  1:2781368/1‑69 (MQ=255)                                             ccGGTAAGTCACACGATCCCCTGCGACGTTGCTGATCAGATCAACAGCACGCTGTGCCAGTTGCGATgg  >  1:2682558/1‑69 (MQ=255)                                             ccGGTAAGTCACACGATCCCCTGCGACGTTGCTGATCAGATCAACAGCACGCTGTGCCAGTTGCGATgg  >  1:2331048/1‑69 (MQ=255)                                             ccGGTAAGTCACACGATCCCCTGCGACGTTGCTGATCAGATCAACAGCACGCTGTGCCAGTTGCGATgg  >  1:1989253/1‑69 (MQ=255)                                             ccGGTAAGTCACACGATCCCCTGCGACGTTGCTGATCAGATCAACAGCACGCTGTGCCAGTTGCGATgg  >  1:1662427/1‑69 (MQ=255)                                             ccGGTAAGTCACACGATCCCCTGCGACGTTGCTGATCAGATCAACAGCACGCTGTGCCAGTTGCGATgg  >  1:1369806/1‑69 (MQ=255)                                             ccGGTAAGTCACACGATCCCCTGCGACGTTGCTGATCAGATCAACAGCACGCTGTGCCAGTTGCGATgg  >  1:1191443/1‑69 (MQ=255)                                                |                                                                  AGCCCCATATAACCTTGCTCACGCAGATCTTCGCCTTTGGTGATCCGATAAGTCACACGATCGCCCGCGACGTTGCTGATCAGATCAACAGCACGCTGTGCCAGTTGCGATGG  >  minE/1688566‑1688678

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑