| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,689,310 | T→G | 47.1% | D38A (GAT→GCT) | yfhJ ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,689,310 | 0 | T | G | 47.1% | ‑6.2 / 81.8 | 68 | D38A (GAT→GCT) | yfhJ | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (17/19); new base G (24/8); total (41/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.61e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01 | |||||||||||
ATCGCTTCGAGGATTTTCTCGTTGGATGCCTGCGGGTCGTCGTCGAAATCTTCCAGATCGCAAATCCACTGATGCATATCGGTGAATCGAACCGTTTTCGGATCAAGATCGGGATACGCATCGTA > minE/1689253‑1689377 | aTCGCTTCGAGGATTTTCTCGTTGGTTGCCTGCGGGTCGTCGTCGAAATCTTCCAGATCGCAAATCCa > 1:2689957/1‑68 (MQ=255)aTCGCTTCGAGGATTTTCTCGTTGGATGCCTGCGGGTCGTCGTCGAAATCTTCCAGATCGCAAATcc > 1:2746900/1‑67 (MQ=255)aTCGCTTCGAGGATTTTCTCGTTGGATGCCTGCGGGTCGTCGTCGAAATCTTCCAGATCGCAAATCCa > 1:1712242/1‑68 (MQ=255) tcgATGCCTGCGGGTCGTCGTCGAAATCTTCCAGAGCGCAAATCCACTGATGCATATCGGTGAATCg < 1:1169314/65‑1 (MQ=255) tcgATGCCTGCGGGTCGTCGTCGAAATCTTCCAGAGCGCAAATCCACTGATGCATATCGGTGAATCg < 1:1705123/65‑1 (MQ=255) tcgATGCCTGCGGGTCGTCGTCGAAATCTTCCAGAGCGCAAATCCACTGATGCATATCGGTGAATCg < 1:2869137/65‑1 (MQ=255) tcgATGCCTGCGGGTCGTCGTCGAAATCTTCCAGAGCGCAAATCCACTGATGCATATCGGTGAATCg < 1:376781/65‑1 (MQ=255) tcgATGCCTGCGGGTCGTCGTCGAAATCTTCCAGAGCGCAAATCCACTGATGCATATCGGTGAATCg < 1:1497021/65‑1 (MQ=255) tcgATGCCTGCGGGTCGTCGTCGAAATCTTCCAGAGCGCAAATCCACTGATGCATATCGGTGAATCg < 1:136966/65‑1 (MQ=255) tcgATGCCTGCGGGTCGTCGTCGAAATCTTCCAGAGCGCAAATCCACTGATGCATATCGGTGAATCg < 1:1106810/65‑1 (MQ=255) tcgATGCCTGCGGGTCGTCGTCGAAATCTTCCAGAGCGCAAATCCACTGATGCATATCGGTGAATCg < 1:959772/65‑1 (MQ=255) gtcgtcgAAATCTTCCAGATCGCAAATCCACTGATGCATATCGGTGAATCg > 1:1489234/1‑51 (MQ=255) gtcgtcgAAATCTTCCAGATCGCAAATCCACTGATGCATATCGGTGAATCGa > 1:636799/1‑52 (MQ=255) gAAATCTTCCAGATCGCAAATCCACTGATGCATATCGGTGAATCGAACCGTTTTCGGATCAAGATCgg > 1:2117749/1‑68 (MQ=255) gAAATCTTCCAGATCGCAAATCCACTGATGCATATCGGTGAATCGAACCGTTTTCGGATCAAGATCgg > 1:2471048/1‑68 (MQ=255) gAAATCTTCCAGATCGCAAATCCACTGATGCATATCGGTGAATCGAACCGTTTTCGGATCAAGATCgg > 1:2758257/1‑68 (MQ=255) gAAATCTTCCAGATCGCAAATCCACTGATGCATATCGGTGAATCGAACCGTTTTCGGATCAAGATCgg > 1:243050/1‑68 (MQ=255) gAAATCTTCCAGATCGCAAATCCACTGATGCATATCGGTGAATCGAACCGTTTTCGGATCAAGATCgg > 1:3290958/1‑68 (MQ=255) gAAATCTTCCAGATCGCAAATCCACTGATGCATATCGGTGAATCGAACCGTTTTCGGATCAAGATCgg > 1:370233/1‑68 (MQ=255) gAAATCTTCCAGATCGCAAATCCACTGATGCATATCGGTGAATCGAACCGTTTTCGGATCAAGATCgg > 1:533181/1‑68 (MQ=255) gAAATCTTCCAGATCGCAAATCCACTGATGCATATCGGTGAATCGAACCGTTTTCGGATCAAGATCgg > 1:1288284/1‑68 (MQ=255) gAAATCTTCCAGATCGCAAATCCACTGATGCATATCGGTGAATCGAACCGTTTTCGGATCAAGATCgg > 1:626247/1‑68 (MQ=255) gAAATCTTCCAGATCGCAAATCCACTGATGCATATCGGCGAATCGAACCGTTTTCGGATCAAGATCgg > 1:1584901/1‑68 (MQ=255) gAAATCTTCCAGATCGCAAATCCACTGAAGCATATCGGTCAATCGAACCGTTTTCGGATCAAGATCgg > 1:1809416/1‑68 (MQ=255) aaaaTCTTCCAGATCGCAAATCCACTGATGCATATCGGTGAATCGAACCGTTTTCGGATCAAGATCgg > 1:2556060/2‑68 (MQ=255) aaTCTTCCAGATCGCAAATCCACTGATGCATATCGGTGAATCGAACCGTTTTCGGATCAAGATCGGGa < 1:2757952/68‑1 (MQ=255) aaTCTTCCAGATCGCAAATCCACTGATGCATATCGGTGAATCGAACCGTTTTCGGATCAAGATCGGGa < 1:1607219/68‑1 (MQ=255) aaTCTTCCAGATCGCAAATCCACTGATGCATATCGGTGAATCGAACCGTTTTCGGATCAAGATCGGGa < 1:3086909/68‑1 (MQ=255) aaTCTTCCAGATCGCAAATCCACTGATGCATATCGGTGAATCGAACCGTTTTCGGATCAAGATCGGGa < 1:3051523/68‑1 (MQ=255) aaTCTTCCAGATCGCAAATCCACTGATGCATATCGGTGAATCGAACCGTTTTCGGATCAAGATCGGGa < 1:302250/68‑1 (MQ=255) aaTCTTCCAGATCGCAAATCCACTGATGCATATCGGTGAATCGAACCGTTTTCGGATCAAGATCGGGa < 1:2246527/68‑1 (MQ=255) aaTCTTCCAGATCGCAAATCCACTGATGCATATCGGTGAATCGAACCGTTTTCGGATCAAGATCGGGa < 1:2924030/68‑1 (MQ=255) aaTCTTCCAGATCGCAAATCCACTGATGCATATCGGTGAATCGAACCGTTTTCGGATCAAGATCGGGa < 1:168407/68‑1 (MQ=255) aaTCTTCCAGATCGCAAATCCACTGATGCATATCGGTGAATCGAACCGTTTTCGGATCAAGATCGGGa < 1:2646063/68‑1 (MQ=255) aaTCTTCCAGATCGCAAATCCACTGATGCATATCGGTGAATCGAACCGTTTTCGGATCAAGATCGGGa < 1:254313/68‑1 (MQ=255) aaTCTTCCAGATCGCAAATCCACTGATGCATATCGGTGAATCGAACCGTTTTCGGATCAAGATCGGGa < 1:2291031/68‑1 (MQ=255) aaTCTTCCAGATCGCAAATCCACTGATGCATATCGGTGAATCGAACCGTTTTCGGATCAAGATCGGGa < 1:863473/68‑1 (MQ=255) aaTCTTCCAGATCGCAAATCCACTGATGCATATCGGTGAATCGAACCGTTTTCGGATCAAGATCGGGa < 1:2220698/68‑1 (MQ=255) aTCTTCGAGAGCGCAAATCCACTGATGCATATCGGTGAATCGAACCGTTTTCGGATCAATATCAGGATa > 1:440273/1‑69 (MQ=255) aTCTTCCAGATCGCAAATCCACTGATGCATATCGGTGAATCGAACCGTTTTCGGATCAAGATCGGGATa < 1:1776835/69‑1 (MQ=255) aTCTTCCAGATCGCAAATCCACTGATGCATATCGGTGAATCGAACCGTTTTCGGATCAAGATCGGGATa < 1:2891307/69‑1 (MQ=255) aTCTTCCAGATCGCAAATCCACTGATGCATATCGGTGAATCGAACCGTTTTCGGATCAAGATCGGGATa < 1:1528806/69‑1 (MQ=255) aTCTTCCAGAGCGCGAATCCACTGATGCATATCGGTGAATCGAACCGTTTTCGGATCAATATCAGGATa > 1:2359243/1‑69 (MQ=255) aTCTTCCAGAGCGCAAATCCACTGATGCATATCGGTGAATCGaa > 1:2737395/1‑44 (MQ=255) aTCTTCCAGAGCGCAAATCCACTGATGCATATCGGTGAATCGAACCGTTTTCGGATCAATATCAGGATa > 1:2929173/1‑69 (MQ=255) aTCTTCCAGAGCGCAAATCCACTGATGCATATCGGTGAATCGAACCGTTTTCGGATCAATATCAGGATa > 1:585492/1‑69 (MQ=255) aTCTTCCAGAGCGCAAATCCACTGATGCATATCGGTGAATCGAACCGTTTTCGGATCAATATCAGGATa > 1:1285224/1‑69 (MQ=255) aTCTTCCAGAGCGCAAATCCACTGATGCATATCGGTGAATCGAACCGTTTTCGGATCAATATCAGGATa > 1:644951/1‑69 (MQ=255) aTCTTCCAGAGCGCAAATCCACTGATGCATATCGGTGAATCGAACCGTTTTCGGATCAATATCAGGATa > 1:710212/1‑69 (MQ=255) aTCTTCCAGAGCGCAAATCCACTGATGCATATCGGTGAATCGAACCGTTTTCGGATCAATATCAGGATa > 1:1526306/1‑69 (MQ=255) aTCTTCCAGAGCGCAAATCCACTGATGCATATCGGTGAATCGAACCGTTTTCGGATCAATATCAGGATa > 1:1178137/1‑69 (MQ=255) aTCTTCCAGAGCGCAAATCCACTGATGCATATCGGTGAATCGAACCGTTTTCGGATCAATATCAGGATa > 1:1282633/1‑69 (MQ=255) aTCTTCCAGAGCGCAAATCCACTGATGCATATCGGTGAATCGAACCGTTTTCGGATCAATATCAGGATa > 1:3332120/1‑69 (MQ=255) aTCTTCCAGAGCGCAAATCCACTGATGCATATCGGTGAATCGAACCGTTTTCGGATCAATATCAGGATa > 1:978079/1‑69 (MQ=255) aTCTTCCAGAGCGCAAATCCACTGATGCATATCGGTGAATCGAACCGTTTTCGGATCAATATCAGGATa > 1:3190278/1‑69 (MQ=255) aTCTTCCAGAGCGCAAATCCACTGATGCATATCGGTGAATCGAACCGTTTTCGGATCAATATCAGGATa > 1:3092274/1‑69 (MQ=255) aTCTTCCAGAGCGCAAATCCACTGATGCATATCGGTGAATCGAACCGTTTTCGGATCAATATCAGGATa > 1:3012744/1‑69 (MQ=255) aTCTTCCAGAGCGCAAATCCACTGATGCATATCGGTGAATCGAACCGTTTTCGGATCAATATCAGGATa > 1:2778982/1‑69 (MQ=255) aTCTTCCAGAGCGCAAATCCACTGATGCATATCGGTGAATCGAACCGTTTTCGGATCAATATCAGGATa > 1:2065933/1‑69 (MQ=255) aTCTTCCAGAGCGCAAATCCACTGATGCATATCGGTGAATCGAACCGTTTTCGGATCAATATCAGGATa > 1:2086788/1‑69 (MQ=255) aTCTTCCAGAGCGCAAATCCACTGATGCATATCGGTGAATCGAACCGTTTTCGGATCAATATCAGGATa > 1:2224905/1‑69 (MQ=255) aTCTTCCAGAGCGCAAATCCACTGATGCATATCGGTGAATCGAACCGTTTTCGGATCAATATCAGGATa > 1:2357111/1‑69 (MQ=255) aTCTTCCAGAGCGCAAATCCACTGATGCATATCGGTGAATCGAACCGTTTTCGGATCAATATCAGGATa > 1:2332531/1‑69 (MQ=255) aTCTTCCAGAGCGCAAATCCACTGATGCATATCGGTGAATCGAACCGTTTTCGGATCAATATCAGGATa > 1:2286603/1‑69 (MQ=255) aTCTTCCAGAGCGCAAATCCACTGATCCATATCGGTGAATCGAACCGTTTTCGGATCAATATCAGGa > 1:1362533/1‑67 (MQ=255) aTCGCAAATCCACTGATGCATATCGGTGAATCGAACCGTTTTCGGATCAAGATCGGGATACGCATCGTa < 1:2897586/69‑1 (MQ=255) aTCGCAAATCCACTGATGCATATCGGTGAATCGAACCGTTTTCGGATCAAGATCGGGATACGCATCGTa < 1:604146/69‑1 (MQ=255) aTCGCAAATCCACTGATGCATATCGGTGAATCGAACCGTTTTCGGATCAAGATCGGGATACGCATCGTa < 1:1814683/69‑1 (MQ=255) | ATCGCTTCGAGGATTTTCTCGTTGGATGCCTGCGGGTCGTCGTCGAAATCTTCCAGATCGCAAATCCACTGATGCATATCGGTGAATCGAACCGTTTTCGGATCAAGATCGGGATACGCATCGTA > minE/1689253‑1689377 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |