| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 130,768 | A→G | 50.0% | L316L (TTA→TTG) | yadE → | predicted polysaccharide deacetylase lipoprotein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 130,768 | 0 | A | G | 50.0% | ‑3.4 / 72.9 | 80 | L316L (TTA→TTG) | yadE | predicted polysaccharide deacetylase lipoprotein |
| Reads supporting (aligned to +/- strand): ref base A (29/11); new base G (19/21); total (48/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.92e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTTGATTTCCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAA > minE/130706‑130836 | tttGATTTCCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGc > 1:2620451/1‑69 (MQ=255) aTTTCCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGt < 1:3066171/68‑1 (MQ=255) aTTTTTTGCATCGGATAGATGGTTATCGGCGGCCTATATTGCTGAGCCGTAGTGAGCACAATATTCTg > 1:2452447/1‑68 (MQ=255) aTTTTTTGCATCGGATAGATGGTTATCGGCGGCCTATATTGCTGAGCCGTAGTGAGCACAATATTCTGt > 1:1342739/1‑69 (MQ=255) aTTTTTTGCATCGGATAGATGGTTATCGGCGGCCTATATTGCTGAGCCGTAGTGAGCACAATATTCTGt > 1:1761935/1‑69 (MQ=255) aTTTTTTGCATCGGATAGATGGTTATCGGCGGCCTATATTGCTGAGCCGTAGTGAGCACAATATTCTGt > 1:1940560/1‑69 (MQ=255) aTTTTTTGCATCGGATAGATGGTTATCGGCGGCCTATATTGCTGAGCCGTAGTGAGCACAATATTCTGt > 1:197627/1‑69 (MQ=255) aTTTTTTGCATCGGATAGATGGTTATCGGCGGCCTATATTGCTGAGCCGTAGTGAGCACAATATTCTGt > 1:1466739/1‑69 (MQ=255) aTTTTTTGCATCGGATAGATGGTTATCGGCGGCCTATATTGCTGAGCCGTAGTGAGCACAATATTCTGt > 1:309454/1‑69 (MQ=255) aTTTTTTGCATCGGATAGATGGTTATCGGCGGCCTATATTGCTGAGCCGTAGTGAGCACAATATTCTGt > 1:3256514/1‑69 (MQ=255) aTTTTTTGCATCGGATAGATGGTTATCGGCGGCCTATATTGCTGAGCCGTAGTGAGCACAATATTCTGt > 1:3279268/1‑69 (MQ=255) aTTTTTTGCATCGGATAGATGGTTATCGGCGGCCTATATTGCTGAGCCGTAGTGAGCACAATATTCTGt > 1:1300204/1‑69 (MQ=255) aTTTTTTGCATCGGATAGATGGTTATCGGCGGCCTATATTGCTGAGCCGTAGTGAGCACAATATTCTGt > 1:2388706/1‑69 (MQ=255) aTTTTTTGCATCGGATAGATGGTTATCGGCGGCCTATATTGCTGAGCCGTAGTGAGCACAATATTCTGt > 1:1079912/1‑69 (MQ=255) aTTTTTTGCATCGGATAGATGGTTATCGGCGGCCTATATTGCTGAGCCGTAGTGAGCACAATATTCTGt > 1:1049846/1‑69 (MQ=255) aTTTTTTGCATAGGATAGATGGTTATCGGCGGCCTATATTGCTGAGCCGTAGTGAGCACAATATTCTGt > 1:3074273/1‑69 (MQ=255) ttttGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGa > 1:1123344/1‑42 (MQ=255) ttttGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTg > 1:473200/1‑69 (MQ=255) ttttGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTg > 1:550340/1‑69 (MQ=255) ttttGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTg > 1:2822523/1‑69 (MQ=255) ttttGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTg > 1:1659862/1‑69 (MQ=255) ttttGCATCGGATAGATGGTTATCGGCGGCCTATATTGCTGAGCCGTa < 1:229415/48‑1 (MQ=255) ttttGCATCGGATAGATGGTTATCGGCGGCCTATATTGCTGAGCCGTa < 1:2506743/48‑1 (MQ=255) ttttGCATCGGATAGATGGTTATCGGCGGCCTATATTGCTGAGCCGTa < 1:2676678/48‑1 (MQ=255) ttttGCATCGGATAGATGGTTATCGGCGGCCTATATTGCTGAGCCGTa < 1:1049094/48‑1 (MQ=255) ttttGCATCGGATAGATGGTTATCGGCGGCCTATATTGCTGAGCCGTa < 1:2677249/48‑1 (MQ=255) ttttGCATCGGATAGATGGTTATCGGCGGCCTATATTGCTGAGCCGTa < 1:2783278/48‑1 (MQ=255) ttttGCATCGGATAGATGGTTATCGGCGGCCTATATTGCTGAGCCGTa < 1:2920247/48‑1 (MQ=255) ttttGCATCGGATAGATGGTTATCGGCGGCCTATATTGCTGAGCCGTa < 1:2930983/48‑1 (MQ=255) ttttGCATCGGATAGATGGTTATCGGCGGCCTATATTGCTGAGCCGTa < 1:3001774/48‑1 (MQ=255) ttttGCATCGGATAGATGGTTATCGGCGGCCTATATTGCTGAGCCGTa < 1:3055156/48‑1 (MQ=255) ttttGCATCGGATAGATGGTTATCGGCGGCCTATATTGCTGAGCCGTa < 1:3413015/48‑1 (MQ=255) ttttGCATCGGATAGATGGTTATCGGCGGCCTATATTGCTGAGCCGTa < 1:714824/48‑1 (MQ=255) ttttGCATCGGATAGATGGTTATCGGCGGCCTATATTGCTGAGCCGTa < 1:93002/48‑1 (MQ=255) ttttGCATCGGATAGATGGTTATCGGCGGCCTATATTGCTGAGCCGTa < 1:2244063/48‑1 (MQ=255) ttttGCATCGGATAGATGGTTATCGGCGGCCTATATTGCTGAGCCGTa < 1:1513064/48‑1 (MQ=255) ttttGCATCGGATAGATGGTTATCGGCGGCCTATATTGCTGAGCCGTa < 1:1385321/48‑1 (MQ=255) ttttGCATCGGATAGATGGTTATCGGCGGCCTATATTGCTGAGCCGTa < 1:216183/48‑1 (MQ=255) ttttGCATCGGATAGATGGTTATCGGCGGCCTATATTGCTGAGCCGTa < 1:108989/48‑1 (MQ=255) ttttGCATCGGATAGATGGTTATCGGCGGCCTATATTGCTGAGCCGTa < 1:1328291/48‑1 (MQ=255) ttttGCATCGGATAGATGGTTATCGGCGGCCTATATTGCTGAGCCGTa < 1:1739439/48‑1 (MQ=255) ttttGCATCGGATAGATGGTTATCGGCGGCCTATATTGCTGAGCCGTa < 1:1876398/48‑1 (MQ=255) ggatAGATGGTTATCGGCGGCCTATATTGCTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCAc > 1:3402962/4‑69 (MQ=255) ggatAGATGGTTATCGGCGGCCTATATTGCTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCAc > 1:1495543/4‑69 (MQ=255) ggatAGATGGTTATCGGCGGCCTATATTGCTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCAc > 1:46366/4‑69 (MQ=255) ggatAGATGGTTATCGGCGGCCTATATTGCTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCAc > 1:2067525/4‑69 (MQ=255) ggatAGATGGTTATCGGCGGCCTATATTGCTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCAc > 1:2162444/4‑69 (MQ=255) tAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGtt < 1:1335104/69‑1 (MQ=255) ggTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTg > 1:2575882/1‑58 (MQ=255) ggTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTg < 1:1891845/58‑1 (MQ=255) ggTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTg > 1:1215901/1‑58 (MQ=255) ggTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTg > 1:3363384/1‑58 (MQ=255) ggTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTg > 1:2426632/1‑58 (MQ=255) ggTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTg > 1:2781839/1‑58 (MQ=255) ggTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTg > 1:135888/1‑58 (MQ=255) ggTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTg > 1:848971/1‑58 (MQ=255) ggTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTg < 1:2861675/58‑1 (MQ=255) ggTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTg > 1:2276658/1‑58 (MQ=255) ggTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTg > 1:149634/1‑58 (MQ=255) aTCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCcgcg < 1:2245514/69‑1 (MQ=255) aTCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCcgcg < 1:273883/69‑1 (MQ=255) aTCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCcgcg < 1:2696447/69‑1 (MQ=255) ttACTGAGCCGTAGTGATCACAATATTCTGTTTGATTTTGCACGTTCACGCCgcgc < 1:1516020/56‑1 (MQ=255) ttACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCgcgc < 1:3352177/56‑1 (MQ=255) ttACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCgcgc < 1:2632034/56‑1 (MQ=255) ttACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCgcgc < 1:462808/56‑1 (MQ=255) ttACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAAttt > 1:1321249/1‑69 (MQ=255) ttACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAAttt > 1:1026780/1‑69 (MQ=255) ttACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAAttt > 1:8980/1‑69 (MQ=255) ttACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAAttt > 1:2298937/1‑69 (MQ=255) ttACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAAttt > 1:750912/1‑69 (MQ=255) ttACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAAttt > 1:734313/1‑69 (MQ=255) ttACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAAttt > 1:2390184/1‑69 (MQ=255) ttACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAAttt > 1:2469907/1‑69 (MQ=255) ttACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAAttt > 1:124193/1‑69 (MQ=255) ttACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAAttt > 1:3349913/1‑69 (MQ=255) ttACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAAttt > 1:2709307/1‑69 (MQ=255) ttACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAAttt > 1:1662746/1‑69 (MQ=255) ttACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAAttt > 1:1391328/1‑69 (MQ=255) ttACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAAttt > 1:1419986/1‑69 (MQ=255) aCTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTaa > 1:2908937/1‑69 (MQ=255) aCTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTa > 1:2052744/1‑68 (MQ=255) | TTTGATTTCCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAA > minE/130706‑130836 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |