| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,690,476 | T→C | 45.8% | P382P (CCA→CCG) | hscA ← | DnaK‑like molecular chaperone specific for IscU |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,690,476 | 0 | T | C | 45.8% | 3.8 / 48.3 | 59 | P382P (CCA→CCG) | hscA | DnaK‑like molecular chaperone specific for IscU |
| Reads supporting (aligned to +/- strand): ref base T (13/19); new base C (19/8); total (32/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.55e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.92e-01 | |||||||||||
CATCGTTTCGAGGCCCAGCGACAGTGGGATCACATCAAGCAACAGCATTTCGCTGTCTGGCTTGTTACCCACCAGAATATCCGCCTGAATCGCCGCGCCAATAGCGACGAC > minE/1690419‑1690529 | cATCGTTTCGAGGCCCAGCGACAGTGGGATCACATCAAGCAACAGCATTTCGCTGTCTGGCTTGTTAcc < 1:2312864/69‑1 (MQ=255)cATCGTTTCGAGGCCCAGCGACAGTGGGATCACATCAAGCAACAGCATTTCGCTGTCTGGCTTGTTAcc < 1:2865068/69‑1 (MQ=255)cATCGTTTCGAGGCCCAGCGACAGTGGGATCACATCAAGCAACAGCATTTCGCTGTCTGGCTTGTTAcc < 1:1756529/69‑1 (MQ=255)cATCGTTTCGAGGCCCAGCGACAGTGGGATCACATCAAGCAACAGCATTTCGCTGTCTGGCTTGTTAcc < 1:123153/69‑1 (MQ=255) aTCGTTTCGAGGCCCAGCGACAGTGGGATCACATCAAGCAACAGCATTTCGCTGTCTGGCTTGTTAcc < 1:2281996/68‑1 (MQ=255) aTCGTTTCGAGGCCCAGCGACAGTGGGATCACATCAAGCAACAGCATTTCGCTGTCTGGCTTGTTAcc < 1:852163/68‑1 (MQ=255) gTTTCGAGGCCCAGCGACAGTGGGATCACATCAAGCAACAGCATTTCGCTGTCTGGCTTGTTAccc > 1:1007978/1‑66 (MQ=255) gTTTCGAGGCCCAGCGACAGTGGGATCACATCAAGCAACAGCATTTCGCTGTCTGGCTTGTTAccc > 1:3312450/1‑66 (MQ=255) gTTTCGAGGCCCAGCGACAGTGGGATCACATCAAGCAACAGCATTTCGCTGTCTGGCTTGTTAccc > 1:604020/1‑66 (MQ=255) gAGGCCCAGCGACAGCGGGATCACATCCAGCAACAGCATTTCGCTGTCCGGCTTGTTACCCACCAGaa < 1:645925/68‑1 (MQ=255) gAGGCCCAGCGACAGCGGGATCACATCCAGCAACAGCATTTCGCTGTCCGGCTTGTTACCCACCAGaa < 1:701922/68‑1 (MQ=255) gAGGCCCAGCGACAGCGGGATCACATCCAGCAACAGCATTTCGCTGTCCGGCTTGTTACCCACCAGaa < 1:1653558/68‑1 (MQ=255) gAGGCCCAGCGACAGCGGGATCACATCCAGCAACAGCATTTCGCTGTCCGGCTTGTTACCCACCAGaa < 1:1201769/68‑1 (MQ=255) gAGGCCCAGCGACAGCGGGATCACATCCAGCAACAGCATTTCGCTGTCCGGCTTGTTACCCACCAGaa < 1:760250/68‑1 (MQ=255) gAGGCCCAGCGACAGCGGGATCACATCCAGCAACAGCATTTCGCTGTCCGGCTTGTTACCCACCAGaa < 1:2723011/68‑1 (MQ=255) gAGGCCCAGCGACAGCGGGATCACATCCAGCAACAGCATTTCGCTGTCCGGCTTGTTACCCACCAGaa < 1:2514860/68‑1 (MQ=255) gAGGCCCAGCGACAGCGGGATCACATCCAGCAACAGCATTTCGCTGTCCGGCTTGTTACCCACCAGaa < 1:819569/68‑1 (MQ=255) gACAGTGGGATCACATCAAGCAACAGCATTTCGCTGTCTGGCTTGTTACCCACCAGAATATCCGCCTGa < 1:3294481/69‑1 (MQ=255) gACAGTGGGATCACATCAAGCAACAGCATTTCGCTGTCTGGCTTGTTACCCACCAGAATATCCGCCTGa < 1:3273610/69‑1 (MQ=255) gACAGTGGGATCACATCAAGCAACAGCATTTCGCTGTCTGGCTTGTTACCCACCAGAATATCCGCCTGa < 1:3199050/69‑1 (MQ=255) gACAGTGGGATCACATCAAGCAACAGCATTTCGCTGTCTGGCTTGTTACCCACCAGAATATCCGCCTGa < 1:2337681/69‑1 (MQ=255) gACAGTGGGATCACATCAAGCAACAGCATTTCGCTGTCTGGCTTGTTACCCACCAGAATATCCGCCTGa < 1:2317794/69‑1 (MQ=255) gACAGTGGGATCACATCAAGCAACAGCATTTCGCTGTCTGGCTTGTTACCCACCAGAATATCCGCCTGa < 1:1010089/69‑1 (MQ=255) gACAGTGGGATCACATCAAGCAACAGCATTTCGCTGTCTGGCTTGTTACCCACCAGAATATCCGCCTGa < 1:2131405/69‑1 (MQ=255) gACAGTGGGATCACATCAAGCAACAGCATTTCGCTGTCTGGCTTGTTACCCACCAGAATATCCGCCTGa < 1:1707408/69‑1 (MQ=255) gACAGTGGGATCACATCAAGCAACAGCATTTCGCTGTCTGGCTTGTTACCCACCAGAATATCCGCCTGa < 1:1786145/69‑1 (MQ=255) gACAGTGGGATCACATCAAGCAACAGCATTTCGCTGTCTGGCTTGTTACCCACCAGAATATCCGCCTGa < 1:1182647/69‑1 (MQ=255) gACAGCGGGATCACATCCAGCAACAGCATTTCGCTGTCCGGCTTGTTACCCACCAGAata > 1:751751/1‑60 (MQ=255) gACAGCGGGATCACATCCAGCAACAGCATTTCGCTGTCCGGCTTGTTACCCACCAGAATATCCGCCTGa > 1:590288/1‑69 (MQ=255) gACAGCGGGATCACATCCAGCAACAGCATTTCGCTGTCCGGCTTGTTACCCACCAGAATATCCGCCTGa > 1:488317/1‑69 (MQ=255) gACAGCGGGATCACATCCAGCAACAGCATTTCGCTGTCCGGCTTGTTACCCACCAGAATATCCGCCTGa > 1:426950/1‑69 (MQ=255) gACAGCGGGATCACATCCAGCAACAGCATTTCGCTGTCCGGCTTGTTACCCACCAGAATATCCGCCTGa > 1:1417889/1‑69 (MQ=255) gACAGCGGGATCACATCCAGCAACAGCATTTCGCTGTCCGGCTTGTTACCCACCAGAATATCCGCCTGa > 1:1452255/1‑69 (MQ=255) gACAGCGGGATCACATCCAGCAACAGCATTTCGCTGTCCGGCTTGTTACCCACCAGAATATCCGCCTGa > 1:137425/1‑69 (MQ=255) gACAGCGGGATCACATCCAGCAACAGCATTTCGCTGTCCGGCTTGTTACCCACCAGAATATCCGCCTGa > 1:717512/1‑69 (MQ=255) gACAGCGGGATCACATCCAGCAACAGCATTTCGCTGTCCGGCTTGTTACCCACCAGAATATCCGCCTGa > 1:1764025/1‑69 (MQ=255) gACAGCGGGATCACATCCAGCAACAGCATTTCGCTGTCCGGCTTGTTACCCACCAGAATATCCGCCTGa > 1:2849937/1‑69 (MQ=255) gACAGCGGGATCACATCCAGCAACAGCATTTCGCTGTCCGGCTTGTTACCCACCAGAATATCCGCCTGa > 1:2680290/1‑69 (MQ=255) gACAGCGGGATCACATCCAGCAACAGCATTTCGCTGTCCGGCTTGTTACCCACCAGAATATCCGCCTGa > 1:1806149/1‑69 (MQ=255) gACAGCGGGATCACATCCAGCAACAGCATTTCGCTGTCCGGCTTGTTACCCACCAGAATATCCGCCTGa > 1:956581/1‑69 (MQ=255) gACAGCGGGATCACATCCAGCAACAGCATTTCGCTGTCCGGCTTGTTACCCACCAGAATATCCGCCTGa > 1:985099/1‑69 (MQ=255) gACAGCGGGATCACATCCAGCAACAGCATTTCGCTGTCCGGCTTGTTACCCACCAGAATATCCGCCTGa > 1:2039060/1‑69 (MQ=255) gACAGCGGGATCACATCCAGCAACAGCATTTCGCTGTCCGGCTTGTTACCCACCAGAATATCCGCCTGa > 1:2307900/1‑69 (MQ=255) gACAGCGGGATCACATCCAGCAACAGCATTTCGCTGTCCGGCTTGTTACCCACCAGAATATCCGCCTGa > 1:2284396/1‑69 (MQ=255) gACAGCGGGATCACATCCAGCAACAGCATTTCGCTGTCCGGCTTGTTACCCACCAGAATATCCGCCTGa > 1:2044506/1‑69 (MQ=255) gACAGCGGGATCACATCCAGAAACAGCATTTCGCTGTCCGGCTTGTTACCCACCAGAATATCCGCCTGa > 1:1261635/1‑69 (MQ=255) aCAGTGGGATCACATCAAGCAACAGCATTTCGCTGTCTGGCTTGTTACCCACCAGAATATCCGCCTGa < 1:2221740/68‑1 (MQ=255) aCAGTGGGATCACATCAAGCAACAGCATTTCGCTGTCTGGCTTGTTACCCACCAGAATATCCGCCTGa < 1:3136759/68‑1 (MQ=255) tGGGATCACATCAAGCAACAGCATTTCGCTGTCTGGCTTGTTACCCACCAGAATATCCGCCTGAATcgc > 1:360649/1‑69 (MQ=255) tGGGATCACATCAAGCAACAGCATTTCGCTGTCTGGCTTGTTACCCACCAGAATATCCGCCTGAATcgc > 1:3272479/1‑69 (MQ=255) cacaTCAAGCAACAGCATTTCGCTGTCTGGCTTGTTACCTACCAGAATATCCGCCTGAATCGCCGCGcc < 1:516232/69‑1 (MQ=255) aGCATTTCGCTGTCTGGCTTGTTACCCACCAGAATATCCGCCTGAATCGCCGCGCCAATAGCgacgac > 1:1614133/1‑68 (MQ=255) aGCATTTCGCTGTCTGGCTTGTTACCCACCAGAATATCCGCCTGAATCGCCGCGCCAATAGCgacgac > 1:112933/1‑68 (MQ=255) aGCATTTCGCTGTCTGGCTTGTTACCCACCAGAATATCCGCCTGAATCGCCGCGCCAATAGCgacgac > 1:1819997/1‑68 (MQ=255) aGCATTTCGCTGTCTGGCTTGTTACCCACCAGAATATCCGCCTGAATCGCCGCGCCAATAGCgacgac > 1:1886664/1‑68 (MQ=255) tCGCTGTCTGGCTTGTTACCCACCAGAATATCCTCCTGAATCGCCGCGCCAATAGcgac > 1:1714921/1‑59 (MQ=255) tCGCTGTCTGGCTTGTTACCCACCAGAATATCCGCCTGAATCGCCGCGCCAATAGcgac > 1:592747/1‑59 (MQ=255) tCGCTGTCTGGCTTGTTACCCACCAGAATATCCGCCTGAATCGCCGCGCCAATAGcgac > 1:284177/1‑59 (MQ=255) tCGCTGTCTGGCTTGTTACCCACCAGAATATCCGCCTGAATCGCCGCGCCAATAGcgac > 1:1044568/1‑59 (MQ=255) | CATCGTTTCGAGGCCCAGCGACAGTGGGATCACATCAAGCAACAGCATTTCGCTGTCTGGCTTGTTACCCACCAGAATATCCGCCTGAATCGCCGCGCCAATAGCGACGAC > minE/1690419‑1690529 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |