| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,693,353 | G→A | 26.6% | L289L (CTG→TTG) | iscS ← | cysteine desulfurase (tRNA sulfurtransferase), PLP‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,693,353 | 0 | G | A | 26.6% | 131.9 / 42.9 | 112 | L289L (CTG→TTG) | iscS | cysteine desulfurase (tRNA sulfurtransferase), PLP‑dependent |
| Reads supporting (aligned to +/- strand): ref base G (31/51); new base A (17/13); total (48/64) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.73e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 112 total aligned reads displayed.
TTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGT > minE/1693291‑1693414 | ttCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTaa > 1:3259728/1‑67 (MQ=255)ttCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTaa > 1:422076/1‑67 (MQ=255)ttCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTaa > 1:1942675/1‑67 (MQ=255)ttCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAAc > 1:1013445/1‑69 (MQ=255)ttCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAAc > 1:2555475/1‑69 (MQ=255)ttCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAAc > 1:712822/1‑69 (MQ=255)ttCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGGTCAGGTAAAc > 1:880744/1‑69 (MQ=255) aCGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAActtc < 1:3267151/68‑1 (MQ=255) aCGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAActtc < 1:2798290/68‑1 (MQ=255) aCGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAActtc < 1:1263460/68‑1 (MQ=255) aCGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAActtc < 1:1784149/68‑1 (MQ=255) aCGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAActtc < 1:1383835/68‑1 (MQ=255) aCGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGGCACCGTTCAGGTAAActtc < 1:2916645/68‑1 (MQ=255) cGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACTGTGTTCCAGGTCACCGTTCAGGTAAActtct > 1:2615313/1‑68 (MQ=255) gTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACttcttc < 1:3118178/69‑1 (MQ=255) gTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACttcttc < 1:106600/69‑1 (MQ=255) aGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACttcttc < 1:2835914/67‑1 (MQ=255) gTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGat < 1:556519/69‑1 (MQ=255) gTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGat < 1:3350421/69‑1 (MQ=255) gAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAActtc > 1:2326359/1‑60 (MQ=255) gAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATc < 1:1521581/69‑1 (MQ=255) gAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATc < 1:635643/69‑1 (MQ=255) gAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATc < 1:307220/69‑1 (MQ=255) gAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATc > 1:2094832/1‑69 (MQ=255) gAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATc > 1:10982/1‑69 (MQ=255) gAAGCTGACGTTGAGAATGTTAGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAActtc > 1:147487/1‑60 (MQ=255) aaGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATc < 1:821626/68‑1 (MQ=255) gCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCttt < 1:3411325/69‑1 (MQ=255) gCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCttt < 1:421853/69‑1 (MQ=255) gCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCttt < 1:318586/69‑1 (MQ=255) gCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCttt < 1:2945517/69‑1 (MQ=255) gCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCttt < 1:650842/69‑1 (MQ=255) gCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCttt < 1:732198/69‑1 (MQ=255) gCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCttt < 1:1012875/69‑1 (MQ=255) gCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCttt < 1:842803/69‑1 (MQ=255) gCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCttt < 1:2814174/69‑1 (MQ=255) gCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCttt < 1:1315766/69‑1 (MQ=255) gCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCttt < 1:1243260/69‑1 (MQ=255) gCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCttt < 1:2054228/69‑1 (MQ=255) gCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCttt < 1:2063159/69‑1 (MQ=255) gCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCttt < 1:2171007/69‑1 (MQ=255) gCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCttt < 1:1498487/69‑1 (MQ=255) gCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCttt < 1:1095598/69‑1 (MQ=255) gCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCttt < 1:1057266/69‑1 (MQ=255) cTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCttt < 1:3075772/68‑1 (MQ=255) gTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAAGTATACTTCTTCGATATCTTTGATGcc < 1:1381951/69‑1 (MQ=255) gTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAAGTAAACTTCTTCGATATCTTTGATGcc < 1:458048/69‑1 (MQ=255) gTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAAGTAAACTTCTTCGATATCTTTGATGcc < 1:2597221/69‑1 (MQ=255) gTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAAGTAAACTTCTTCGATATCTTTGATGcc < 1:915601/69‑1 (MQ=255) gTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAAGTAAACTTCTTCGATATCTTTGATGcc < 1:2995764/69‑1 (MQ=255) gTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAAGTAAACTTCTTCGATATCTTTGATGcc < 1:3179554/69‑1 (MQ=255) gTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAAGTAAACTTCTTCGATATCTTTGATGcc < 1:2477515/69‑1 (MQ=255) gTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAAGTAAACTTCTTCGATATCTTTGATGcc < 1:2119572/69‑1 (MQ=255) gTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAAGTAAACTTCTTCGATATCTTTGATGcc < 1:704275/69‑1 (MQ=255) gTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAAGTAAACTTCTTCGATATCTTTGATGcc < 1:896030/69‑1 (MQ=255) gTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAAGTAAACTTCTTCGATATCTTTGATGcc < 1:2517664/69‑1 (MQ=255) tGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAAGTAAACTTCTTCGATATCTTTGATGcc < 1:394538/67‑1 (MQ=255) gagaATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGt > 1:3224759/1‑68 (MQ=255) gagaATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGt > 1:1777242/1‑68 (MQ=255) tGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATc > 1:166800/1‑52 (MQ=255) tGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATc > 1:2982652/1‑52 (MQ=255) tGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATc > 1:544385/1‑52 (MQ=255) tGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATc > 1:2631486/1‑52 (MQ=255) tGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATc > 1:2645372/1‑52 (MQ=255) tGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATc > 1:2678005/1‑52 (MQ=255) ttCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAgc > 1:1241940/1‑68 (MQ=255) ttCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGa > 1:766495/1‑69 (MQ=255) ttCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGa > 1:2288736/1‑69 (MQ=255) ttCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGa > 1:1611576/1‑69 (MQ=255) ttCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGa > 1:592853/1‑69 (MQ=255) ttCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGa > 1:2684431/1‑69 (MQ=255) ttCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGa > 1:2182492/1‑69 (MQ=255) ttCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGa > 1:1655871/1‑69 (MQ=255) ttCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGa > 1:2840956/1‑69 (MQ=255) gtgtTCCAGGTCACCGTTCAAGTAAACTTCTTCGATATCTTTGTTGCCGATCCACAGACGGTTACGCAg > 1:2941170/1‑69 (MQ=255) gtgtTCCAGGTCACCGTTCAAGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAg > 1:680175/1‑69 (MQ=255) gtgtTCCAGGTCACCGTTCAAGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAg > 1:83140/1‑69 (MQ=255) gtgtTCCAGGTCACCGTTCAAGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAg > 1:1212015/1‑69 (MQ=255) gtgtTCCAGGTCACCGTTCAAGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAg > 1:959945/1‑69 (MQ=255) gtgtTCCAGGTCACCGTTCAAGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAg > 1:1562965/1‑69 (MQ=255) gtgtTCCAGGTCACCGTTCAAGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAg > 1:3387542/1‑69 (MQ=255) gtgtTCCAGGTCACCGTTCAAGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAg > 1:1521722/1‑69 (MQ=255) gtgtTCCAGGTCACCGTTCAAGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAg > 1:3323282/1‑69 (MQ=255) gtgtTCCAGGTCACCGTTCAAGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAg > 1:3296672/1‑69 (MQ=255) gtgtTCCAGGTCACCGTTCAAGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAg > 1:2328066/1‑69 (MQ=255) gtgtTCCAGGTCACCGTTCAAGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAg > 1:22307/1‑69 (MQ=255) gtgtTCCAGGTCACCGTTCAAGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAg > 1:2190961/1‑69 (MQ=255) gtgtTCCAGGTCACCGTTCAAGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAg > 1:2124246/1‑69 (MQ=255) gtgtTCCAGGTCACCGTTCAAGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAg > 1:1799562/1‑69 (MQ=255) cAGGTCACCGTTCAGGTAAACTTCTTCGATATCttt < 1:950107/36‑1 (MQ=255) cGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGt < 1:223578/68‑1 (MQ=255) cGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGt < 1:2498517/68‑1 (MQ=255) cGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGt < 1:2980795/68‑1 (MQ=255) cGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGt < 1:755698/68‑1 (MQ=255) cGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGt < 1:314713/68‑1 (MQ=255) cGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGt < 1:3147292/68‑1 (MQ=255) cGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGt < 1:3251426/68‑1 (MQ=255) cGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGt < 1:3274579/68‑1 (MQ=255) cGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGt < 1:1618123/68‑1 (MQ=255) gTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGt < 1:606021/67‑1 (MQ=255) | TTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGT > minE/1693291‑1693414 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |