Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	1,696,724	T→C	53.7%	R199R (CGT→CGC)	suhB →	inositol monophosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,696,724	0	T	C	53.7%	2.4 / 31.4	41	R199R (CGT→CGC)	suhB	inositol monophosphatase
Reads supporting (aligned to +/- strand):  ref base T (13/6);  new base C (6/16);  total (19/22)
Fisher's exact test for biased strand distribution p-value = 1.27e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GAATGTGCAGACTTCCGTCGTACCGGTTCTGCGGCGCTGGATCTGGCTTACGTCGCTGCGGGTCGTGTTGACGGTTTCTTTGAAATCGGTCTGCGCCCGTGGGACTTCGCCGCAG  >  minE/1696659‑1696773                                                                  |                                                  gAATGTGCAGACTTCCGTCGTACCGGTTCTGCGGCGCTGGATCTGGCTTACGTCGCTGCGGGTCGTGtt                                                <  1:2085902/69‑1 (MQ=255) gAATGTGCAGACTTCCGTCGTACCGGTTCTGCGGCGCTGGATCTGGCTTACGTCGCTGCGGGTCGTGtt                                                <  1:1667025/69‑1 (MQ=255)    tgtgCAGACTTCCGTCGTACCGGTTCTGCGGCGCTGGATCTGGCTTACGTCGCTGCGGGTCGTGTTGAc                                             >  1:3194368/1‑69 (MQ=255)    tgtgCAGACTTCCGTCGTACCGGTTCTGCGGCGCTGGATCTGGCTTACGTCGCTGCGGGTCGTGTTGAc                                             >  1:2977183/1‑69 (MQ=255)    tgtgCAGACTTCCGTCGTACCGGTTCTGCGGCGCTGGATCTGGCTTACGTCGCTGCGGGTCGTGTTGAc                                             >  1:1776863/1‑69 (MQ=255)     gtgCAGACTTCCGTCGTACCGGTTCTGCGGCGCTGGATCTGGCTTACGTCGCTGCGGGTCGTGtt                                                <  1:2444266/65‑1 (MQ=255)        cAGACTTCCGTCGTACCGGTTCTGCGGCGCTGGATCTGGCTTACGTCGCTGCGGGTCGTGTTGACGGtt                                         >  1:2238799/1‑69 (MQ=255)        cAGACTTCCGTCGTACCGGTTCTGCGGCGCTGGATCTGGCTTACGTCGCTGCGGGTCGTGTTGACGGtt                                         >  1:779847/1‑69 (MQ=255)        cAGACTTCCGTCGTACCGGATCTGCGGCGCTGGATCTGGCTTACGTCGCTGCGGGTCGTGTTGACGGtt                                         >  1:1320054/1‑69 (MQ=255)               ccGTCGTACCGGTTCTGCGGCGCTGGATCTGGCTTACGTCGCTGCGGGTCGTGTTGACGGTTTCTTTg                                   >  1:634027/1‑68 (MQ=255)               ccGTCGTACCGGTTCTGCGGCGCTGGATCTGGCTTACGTCGCTGCGGGTCGTGTTGACGGTTTCTTTGa                                  >  1:535721/1‑69 (MQ=255)               ccGTCGTACCGGTTCTGCGGCGCTGGATCTGGCTTACGTCGCTGCGGGTCGTGTTGACGGTTTCTTTGa                                  >  1:1143907/1‑69 (MQ=255)               ccGTCGTACCGGTTCTGCGGCGCTGGATCTGGCTTACGTCGCTGCGGGTCGTGTTGACGGTTTCTTTGa                                  >  1:788396/1‑69 (MQ=255)               ccGTCGTACCGGTTCTGCGGCGCTGGATCTGGCTTACGTCGCTGCGGGTCGTGTTGACGGTTTCTTTGa                                  >  1:2588878/1‑69 (MQ=255)                       ccGGTTCTGCGGCGCTGGATCTGGCTTACGTCGCTGCGGGTCGTGTTGACgg                                           >  1:2334186/1‑52 (MQ=255)                       ccGGTTCTGCGGCGCTGGATCTGGCTTACGTCGCTGCGGGTCGTGTTGACGGTTTCTTTGa                                  >  1:2699010/1‑61 (MQ=255)                         ggTTCTGCGGCGCTGGATCTGGCTTACGTCGCTGCGGGTCGTGTTGACGGTTTCTTTGAAATCGGTCTg                        <  1:45063/69‑1 (MQ=255)                         ggTTCTGCGGCGCTGGATCTGGCTTACGTCGCTGCGGGTCGTGTTGACGGTTTCTTTGAAATCGGTCTg                        <  1:1634711/69‑1 (MQ=255)                         ggTTCTGCGGCGCTGGATCTGGCTTACGTCGCTGCGGGTCGTGTTGACGGGTTCTTTGAAATCGGTCTg                        <  1:3203119/69‑1 (MQ=255)                                          tCTGGCTTACGTCGCTGCGGGTCGCTTTGACGGTTTCttt                                    <  1:483928/40‑1 (MQ=38)                                          tCTGGCTTACGTCGCTGCGGGTCGCGTTGACGGTTTCttt                                    <  1:1021532/40‑1 (MQ=255)                                          tCTGGCTTACGTCGCTGCGGGTCGCGTTGACGGTTTCttt                                    <  1:964798/40‑1 (MQ=255)                                          tCTGGCTTACGTCGCTGCGGGTCGCGTTGACGGTTTCttt                                    <  1:552270/40‑1 (MQ=255)                                          tCTGGCTTACGTCGCTGCGGGTCGCGTTGACGGTTTCttt                                    <  1:468344/40‑1 (MQ=255)                                          tCTGGCTTACGTCGCTGCGGGTCGCGTTGACGGTTTCttt                                    <  1:385981/40‑1 (MQ=255)                                          tCTGGCTTACGTCGCTGCGGGTCGCGTTGACGGTTTCttt                                    <  1:3420331/40‑1 (MQ=255)                                          tCTGGCTTACGTCGCTGCGGGTCGCGTTGACGGTTTCttt                                    <  1:677234/40‑1 (MQ=255)                                          tCTGGCTTACGTCGCTGCGGGTCGCGTTGACGGTTTCttt                                    <  1:2847739/40‑1 (MQ=255)                                          tCTGGCTTACGTCGCTGCGGGTCGCGTTGACGGTTTCttt                                    <  1:2731401/40‑1 (MQ=255)                                          tCTGGCTTACGTCGCTGCGGGTCGCGTTGACGGTTTCttt                                    <  1:2620927/40‑1 (MQ=255)                                          tCTGGCTTACGTCGCTGCGGGTCGCGTTGACGGTTTCttt                                    <  1:2188213/40‑1 (MQ=255)                                          tCTGGCTTACGTCGCTGCGGGTCGCGTTGACGGTTTCttt                                    <  1:2054930/40‑1 (MQ=255)                                          tCTGGCTTACGTCGCTGCGGGTCGCGTTGACGGTTTCttt                                    <  1:1954470/40‑1 (MQ=255)                                          tCTGGCTTACGTCGCTGCGGGTCGCGTTGACGGTTTCttt                                    <  1:1882844/40‑1 (MQ=255)                                          tCTGGCTTACGTCGCTGCGGGTCGCGTTGACGGTTTCttt                                    <  1:1638546/40‑1 (MQ=255)                                                ttACGTCGCTGCGGGTCGCGTTGACGGTTTCTTTGAAATCGGTCTGCGCCCGTGGGATTTCGCGGCAg  >  1:3023594/1‑68 (MQ=255)                                                ttACGTCGCTGCGGGTCGCGTTGACGGTTTCTTTGAAATCGGTCTGCGCCCGTGGGATTTCGCGGCAg  >  1:2982355/1‑68 (MQ=255)                                                ttACGTCGCTGCGGGTCGCGTTGACGGTTTCTTTGAAATCGGTCTGCGCCCGTGGGATTTCGCGGCAg  >  1:2927737/1‑68 (MQ=255)                                                ttACGTCGCTGCGGGTCGCGTTGACGGTTTCTTTGAAATCGGTCTGCGCCCGTGGGATTTCGCGGCAg  >  1:941468/1‑68 (MQ=255)                                                ttACGTCGCTGCGGGTCGCGTTGACGGTTTCTTTGAAATCGGTCTGCGCCCGTGGGATTTCGCGGCAg  >  1:1493247/1‑68 (MQ=255)                                                ttACGTCGCTGCGGGTCGCGGTGACGGTTTCTTTGAAATCGGTCTGCGCCCGTGGGATTTCGCGGCAg  >  1:3127373/1‑68 (MQ=255)                                                                  |                                                  GAATGTGCAGACTTCCGTCGTACCGGTTCTGCGGCGCTGGATCTGGCTTACGTCGCTGCGGGTCGTGTTGACGGTTTCTTTGAAATCGGTCTGCGCCCGTGGGACTTCGCCGCAG  >  minE/1696659‑1696773

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑