| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 21,520 | G→A | 46.6% | E484E (GAG→GAA) | ileS → | isoleucyl‑tRNA synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 21,520 | 0 | G | A | 46.6% | 28.5 / 139.7 | 163 | E484E (GAG→GAA) | ileS | isoleucyl‑tRNA synthetase |
| Reads supporting (aligned to +/- strand): ref base G (44/43); new base A (38/38); total (82/81) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.42e-01 | |||||||||||
Only 100 of 163 total aligned reads displayed.
TCTCCCGTCAGCGCACCTGGGGTGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTCGA > minE/21459‑21576 | tctcCCGTCAGCGCACCTGGGGTGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATc > 1:1158733/1‑69 (MQ=255)tctcCCGTCAGCGCACCTGGGGTGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATc > 1:1753556/1‑69 (MQ=255)tctcCCGTCAGCGCACCTGGGGTGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATc > 1:2161995/1‑69 (MQ=255)tctcCCGTCAGCGCACCTGGGGTGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATc > 1:2339801/1‑69 (MQ=255) ccTGGGGCGTGCCGATGTCACTGTTCGTGCACAAAGACACAGAAGAACTGc > 1:494775/1‑51 (MQ=255) ccTGGGGCGTGCCGATGTCACTGTTCGTGCACAAAGACACAGAAGAACTGCATCCGCGt > 1:3230271/1‑59 (MQ=255) ccTGGGGCGTGCCGATGTCACTGTTCGTGCACAAAGACACAGAAGAACTGCATCCGCGTa > 1:2996985/1‑60 (MQ=255) ccTGGGGCGTGCCGATGTCACTGTTCGTGCACAAAGACACAGAAGAACTGCATCCGCGTAc > 1:1878251/1‑61 (MQ=255) ccTGGGGCGTGCCGATGTCACTGTTCGTGCACAAAGACACAGAAGAACTGCATCCGCGTAc > 1:595943/1‑61 (MQ=255) ccTGGGGCGTGCCGATGTCACTGTTCGTGCACAAAGACACAGAAGAACTGCATCCGCGTAc > 1:2107011/1‑61 (MQ=255) ccTGGGGCGTGCCGATGTCACTGTTCGTGCACAAAGACACAGAAGAACTGCATCCGCGTAc > 1:2122357/1‑61 (MQ=255) ccTGGGGCGTGCCGATGTCACTGTTCGTGCACAAAGACACAGAAGAACTGCATCCGCGTAc > 1:516987/1‑61 (MQ=255) ccTGGGGCGTGCCGATGTCACTGTTCGTGCACAAAGACACAGAAGAACTGCATCCGCGTAc > 1:2279261/1‑61 (MQ=255) ccTGGGGCGTGCCGATGTCACTGTTCGTGCACAAAGACACAGAAGAACTGCATCCGCGTAc > 1:1655299/1‑61 (MQ=255) ccTGGGGCGTGCCGATGTCACTGTTCGTGCACAAAGACACAGAAGAACTGCATCCGCGTAc > 1:1594664/1‑61 (MQ=255) ccTGGGGCGTGCCGATGTCACTGTTCGTGCACAAAGACACAGAAGAACTGCATCCGCGTAc > 1:813406/1‑61 (MQ=255) ccTGGGGCGTGCCGATGTCACTGTTCGTGCACAAAGACACAGAAGAACTGCATCCGCGTAc > 1:86549/1‑61 (MQ=255) ccTGGGGCGTGCCGATGTCACTGTTCGTGCACAAAGACACAGAAGAACTGCATCCGCGTAc > 1:2452393/1‑61 (MQ=255) ccTGGGGCGTGCCGATGTCACTGTTCGTGCACAAAGACACAGAAGAACTGCATCCGCGTAc > 1:430782/1‑61 (MQ=255) ccTGGGGCGTGCCGATGTCACTGTTCGTGCACAAAGACACAGAAGAACTGCATCCGCGTAc > 1:399324/1‑61 (MQ=255) ccTGGGGCGTGCCGATGTCACTGTTCGTGCACAAAGACACAGAAGAACTGCATCCGCGTAc > 1:3398395/1‑61 (MQ=255) ccTGGGGCGTGCCGATGTCACTGTTCGTGCACAAAGACACAGAAGAACTGCATCCGCGTAc > 1:336470/1‑61 (MQ=255) ccTGGGGCGTGCCGATGTCACTGTTCGTGCACAAAGACACAGAAGAACTGCATCCGCGTAc > 1:1253571/1‑61 (MQ=255) cTGGGGTGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACt < 1:2287326/69‑1 (MQ=255) cTGGGGTGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACt < 1:459185/69‑1 (MQ=255) cTGGGGTGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACt < 1:3254784/69‑1 (MQ=255) cTGGGGTGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACt < 1:3110369/69‑1 (MQ=255) cTGGGGTGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACt < 1:1914394/69‑1 (MQ=255) cTGGGGTGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACt < 1:1302675/69‑1 (MQ=255) cTGGGGTGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACt < 1:1672705/69‑1 (MQ=255) cTGGGGTGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACt < 1:1034988/69‑1 (MQ=255) tgtACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTg > 1:628209/1‑64 (MQ=255) tACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGgaag < 1:533156/69‑1 (MQ=255) tACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGgaag < 1:2950418/69‑1 (MQ=255) tACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGga > 1:651498/1‑67 (MQ=255) tACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGga > 1:3314107/1‑67 (MQ=255) tACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGga > 1:918846/1‑67 (MQ=255) tACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGga > 1:2410449/1‑67 (MQ=255) tACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGga > 1:1685653/1‑67 (MQ=255) aCCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGgaag < 1:500221/68‑1 (MQ=255) aCCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGgaag < 1:1378824/68‑1 (MQ=255) ccGATGTCACTGTTCGTGCTCAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGgaagaa > 1:1437726/1‑69 (MQ=255) ccGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCcgc > 1:1370482/1‑46 (MQ=255) ccGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGgaagaa > 1:2369669/1‑69 (MQ=255) ccGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGgaagaa > 1:723023/1‑69 (MQ=255) ccGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGgaagaa > 1:1415621/1‑69 (MQ=255) ccGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGgaagaa > 1:3197474/1‑69 (MQ=255) ccGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGgaagaa > 1:3089427/1‑69 (MQ=255) ccGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGgaagaa > 1:2534389/1‑69 (MQ=255) ccGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGgaagaa > 1:3268165/1‑69 (MQ=255) ccGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGgaagaa > 1:2847012/1‑69 (MQ=255) ccGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGgaagaa > 1:707216/1‑69 (MQ=255) ccGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGgaagaa > 1:608487/1‑69 (MQ=255) cACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGGAAGAAGTGGCaa > 1:1306761/1‑69 (MQ=255) cACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGGAAGAAGTGGCaa > 1:2730249/1‑69 (MQ=255) aCTGTTCGTGCACAAAGACACAGAAGAACTGCATCCGCGTACTCTCGAACTGATGGAAGAAGTGGCaaa < 1:139521/69‑1 (MQ=255) aCTGTTCGTGCACAAAGACACAGAAGAACTGCATCCGCGTACTCTCGAACTGATGGAAGAAGTGGCaaa < 1:1049683/69‑1 (MQ=255) aCTGTTCGTGCACAAAGACACAGAAGAACTGCATCCGCGTACTCTCGAACTGATGGAAGAAGTGGCaaa < 1:259517/69‑1 (MQ=255) aCTGTTCGTGCACAAAGACACAGAAGAACTGCATCCGCGTACTCTCGAACTGATGGAAGAAGTGGCaaa < 1:2413054/69‑1 (MQ=255) aCTGTTCGTGCACAAAGACACAGAAGAACTGCATCCGCGTACTCTCGAACTGATGGAAGAAGTGGCaaa < 1:2209571/69‑1 (MQ=255) aCTGTTCGTGCACAAAGACACAGAAGAACTGCATCCGCGTACTCTCGAACTGATGGAAGAAGTGGCaaa < 1:1826942/69‑1 (MQ=255) gTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGGAAGAAGTGGCAAAAcg > 1:305712/1‑69 (MQ=255) ttCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGGAAGAAGTGGCGAAAcgc > 1:1811084/1‑69 (MQ=255) ttCGTGCACAAAGACACAGAAGAACTGCATCCGCGTACTCTCGAACTGATg < 1:3425864/51‑1 (MQ=255) ttCGTGCACAAAGACACAGAAGAACTGCATCCGCGTACTCTCGAACTGATg < 1:829997/51‑1 (MQ=255) ttCGTGCACAAAGACACAGAAGAACTGCATCCGCGTACTCTCGAACTGATg < 1:796001/51‑1 (MQ=255) ttCGTGCACAAAGACACAGAAGAACTGCATCCGCGTACTCTCGAACTGATg < 1:979049/51‑1 (MQ=255) ttCGTGCACAAAGACACAGAAGAACTGCATCCGCGTACTCTCGAACTGATg < 1:3070463/51‑1 (MQ=255) ttCGTGCACAAAGACACAGAAGAACTGCATCCGCGTACTCTCGAACTGATg < 1:1470631/51‑1 (MQ=255) ttCGTGCACAAAGACACAGAAGAACTGCATCCGCGTACTCTCGAACTGATg < 1:1689633/51‑1 (MQ=255) ttCGTGCACAAAGACACAGAAGAACTGCATCCGCGTACTCTCGAACTGATg < 1:1994706/51‑1 (MQ=255) ttCGTGCACAAAGACACAGAAGAACTGCATCCGCGTACTCTCGAACTGATg < 1:2072118/51‑1 (MQ=255) ttCGTGCACAAAGACACAGAAGAACTGCATCCGCGTACTCTCGAACTGATg < 1:2229038/51‑1 (MQ=255) ttCGTGCACAAAGACACAGAAGAACTGCATCCGCGTACTCTCGAACTGATg < 1:2278536/51‑1 (MQ=255) ttCGTGCACAAAGACACAGAAGAACTGCATCCGCGTACTCTCGAACTGATg < 1:2489018/51‑1 (MQ=255) ttCGTGCACAAAGACACAGAAGAACTGCATCCGCGTACTCTCGAACTGATg < 1:2948022/51‑1 (MQ=255) cGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGGAAGAAGTGGCAAAAcgcg > 1:1142295/1‑68 (MQ=255) cGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGGAAGAAGTGGCAAAAcgcg > 1:183494/1‑68 (MQ=255) cGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGGAAGAAGTGGCAAAAcgcg > 1:3391175/1‑68 (MQ=255) cGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGGAAGAAGTGGCAAAAcgcg > 1:1324755/1‑68 (MQ=255) cGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACT‑ATGGAAGAAGTGGCAAAACGCGtt < 1:2936755/69‑1 (MQ=255) tGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGATCTGATGGAAGAAGTGGCAAAACGCGtt < 1:268201/68‑1 (MQ=255) tGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGGAAGAAGTGGCAAAACGCGtt < 1:1447911/68‑1 (MQ=255) tGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGGAAGAAGTGGCAAAACGCGtt < 1:580221/68‑1 (MQ=255) tGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGGAAGAAGTGGCAAAACGCGtt < 1:2097905/68‑1 (MQ=255) tGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGGAAGAAGTGGCAAAACGCGtt < 1:1541474/68‑1 (MQ=255) tGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGGAAGAAGTGGCAAAACGCGtt < 1:2597201/68‑1 (MQ=255) acaAAGACACAGAAGAACTGCATCCGCGTACTCTCGAACTGATg < 1:2546756/44‑1 (MQ=255) caAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGGAAGAAGTGGCAAAACGCGtt < 1:2526980/64‑1 (MQ=255) aaaGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTc < 1:1441852/69‑1 (MQ=255) aaaGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTc < 1:3196549/69‑1 (MQ=255) aaaGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTc < 1:760468/69‑1 (MQ=255) aaaGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTc < 1:2905767/69‑1 (MQ=255) aaaGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTc < 1:2625284/69‑1 (MQ=255) aGACACAGAAGAACTGCATCCGCGTACTCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTGa > 1:665643/1‑69 (MQ=255) aGACACAGAAGAACTGCATCCGCGTACTCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTGa > 1:2053686/1‑69 (MQ=255) aGACACAGAAGAACTGCATCCGCGTACTCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTGa > 1:3340560/1‑69 (MQ=255) aGACACAGAAGAACTGCATCCGCGTACTCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTGa > 1:2378297/1‑69 (MQ=255) aGACACAGAAGAACTGCATCCGCGTACTCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTGa > 1:2455100/1‑69 (MQ=255) aGACACAGAAGAACTGCATCCGCGTACTCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTGa > 1:2645525/1‑69 (MQ=255) | TCTCCCGTCAGCGCACCTGGGGTGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTCGA > minE/21459‑21576 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |