| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,698,435 | A→T | 58.7% | Q105H (CAA→CAT) | csiE → | stationary phase inducible protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,698,435 | 0 | A | T | 58.7% | 25.5 / 61.3 | 63 | Q105H (CAA→CAT) | csiE | stationary phase inducible protein |
| Reads supporting (aligned to +/- strand): ref base A (9/17); new base T (20/17); total (29/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.99e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.73e-01 | |||||||||||
CAACGTTTGTGTTTATTTCACTGGCTACGACGTGGTTTCCGTCTGTGTCCGTCATTTATTACCAGCCAATTCACCCCCGCCCTGAAGAGTGAACTGAAGCGGCGCGGAATTGCGCGTAACTTTTACGACGATAC > minE/1698367‑1698500 | cAACGTTTGTGTTTATTTCACTGGCTACGACGTGGTTTCCGTCTGTGTCCGTCATTTATTACCAGCCaa < 1:926489/69‑1 (MQ=255)cAACGTTTGTGTTTATTTCACTGGCTACGACGTGGTTTCCGTCTGTGTCCGTCATTTATTACCAGCCaa < 1:1926529/69‑1 (MQ=255)cAACGTTTGTGTTTATTTCACTGGCTACGACGTGGTTTCCGTCTGTGTCCGTCATTTATTACCAGCCaa < 1:1860605/69‑1 (MQ=255)cAACGTTTGTGTTTATTTCACTGGCTACGACGTGGTTTCCGTCTGTGTCCGTCATTTATTACCAGCCaa < 1:3358091/69‑1 (MQ=255)cAACGTTTGTGTTTATTTCACTGGCTACGACGTGGTTTCCGTCTGTGTCCGTCATTTATTACCAGCCaa < 1:332048/69‑1 (MQ=255)cAACGTTTGTGTTTATTTCACTGGCTACGACGTGGTTTCCGTCTGTGTCCGTCATTTATTACCAGCCaa < 1:1568594/69‑1 (MQ=255)cAACGTTTGTGTTTATTTCACTGGCTACGACGTGGTTTCCGTCTGTGTCCGTCATTTATTACCAGCCaa < 1:2417968/69‑1 (MQ=255)cAACGTTTGTGTTTATTTCACTGGCTACGACGTGGTTTCCGTCTGTGTCCGTCATTTATTACCAGCCaa < 1:2538076/69‑1 (MQ=255)cAACGTTTGTGTTTATTTCACTGGCTACGACGTGGTTTCCGTCTGTGTCCGTCATTTATTACCAGCCaa < 1:3058574/69‑1 (MQ=255)cAACGTTTGTGTTTATTTCACTGGCTACGACGTGGTTTCCGTCTGTGTCCGTCATTTATTACCAGCCaa < 1:296478/69‑1 (MQ=255) gTTTGTGTTTATTTCACTGGCTACGACGTGG‑TTCCGTCTGTGTCCGTCATTTATTACCAGCCAATTCAc < 1:2641892/69‑1 (MQ=255) tttGTGTTTATTTCACTGGCTACGACGTGGTTTCCGTCTGTGTCCGTCATTTATTACCAGCCAATTCAc < 1:3274585/69‑1 (MQ=255) tttGTGTTTATTTCACTGGCTACGACGTGGTTTCCGTCTGTGTCCGTCATTTATTACCAGCCAATTCAc < 1:1469334/69‑1 (MQ=255) gtgtTTATTTCACTGGCTACGACGTGGTTTCCGTCTGTGTCCGTCATTTATTACCAGCCAttt > 1:412009/1‑63 (MQ=255) gtgtTTATTTCACTGGCTACGACGTGGTTTCCGTCTGTGTCCGTCATTTATTACCAGCCATTTCAcccc > 1:3177045/1‑69 (MQ=255) gtgtTTATTTCACTGGCTACGACGTGGTTTCCGTCTGTGTCCGTCATTTATTACCAGCCATTTCAcccc > 1:2337881/1‑69 (MQ=255) gtgtTTATTTCACTGGCTACGACGTGGTTTCCGTCTGTGTCCGTCATTTATTACCAGCCATTTCAcccc > 1:3360989/1‑69 (MQ=255) gtgtTTATTTCACTGGCTACGACGTGGTTTCCGTCTGTGTCCGTCATTTATTACCAGCCATTTCAcccc > 1:1952785/1‑69 (MQ=255) gtgtTTATTTCACTGGCTACGACGTGGTTTCCGTCTGTGTCCGTCATTTATTACCAGCCATTTCAcccc > 1:194029/1‑69 (MQ=255) gtgtTTATTTCACTGGCTACGACGTGGTTTCCGTCTGTGTCCGTCATTTATTACCAGCCATTTCAcccc > 1:294709/1‑69 (MQ=255) gtgtTTATTTCACTGGCTACGACGTGGTTTCCGTCTGTGTCCGTCATTTATTACCAGCCATTTCAcccc > 1:1648985/1‑69 (MQ=255) gtgtTTATTTCACTGGCTACGACGTGGTTTCCGTCTGTGTCCGTCATTTATTACCAGCCATTTCAcccc > 1:560106/1‑69 (MQ=255) gtgtTTATTTCACTGGCTACGACGTGGTTTCCGTCTGTGTCCGTCATTTATTACCAGCCATTTCAcccc > 1:609141/1‑69 (MQ=255) gtgtTTATTTCACTGGCTACGACGTGGTTTCCGTCTGTGTCCGTCATTTATTACCAGCCATTTCAcccc > 1:1444246/1‑69 (MQ=255) gtgtTTATTTCACTGGCTACGACGTGGTTTCCGTCTGTGTCCGTCATTTATTACCAGCCATTTCAccc > 1:914660/1‑68 (MQ=255) gtgtTTATTTCACTGGCTACGACGTGGTTTCCGTCTGTGTCCGTCATTTATTACCAGCCATTTCAccc > 1:3091019/1‑68 (MQ=255) aCTGGCTACGACGTGGTTTCCGTCTGTGTCCGTCATTTATTACCAGCCAATTCACCCCCGCCCTGAag < 1:796642/68‑1 (MQ=255) ggCTACGACGTGGTTTCCGTCTGTGTCCGTCATTTATTACCAGCCAATTCACCCCCGCCCTGAAGAGTg < 1:407501/69‑1 (MQ=255) ggCTACGACGTGGTTTCCGTCTGTGTCCGTCATTTATTACCAGCCAATTCACCCCCGCCCTGAAGAGTg < 1:2174630/69‑1 (MQ=255) ggCTACGACGTGGTTTCCGTCTGTGTCCGTCATTTATTACCAGCCAATTCACCCCCGCCCTGAAGAGTg < 1:708009/69‑1 (MQ=255) ggCTACGACGTGGTTTCCGTCTGTGTCCGTCATTTATTACCAGCCAATTCACCCCCGCCCTGAAGAGTg < 1:1920497/69‑1 (MQ=255) gacgTGGTTTCCGTCTGTGTCCGTCATTTATTACCAGCCATTTCACCCCCGCCCTGAAGAGt > 1:2586310/1‑62 (MQ=255) gacgTGGTTTCCGTCTGTGTCCGTCATTTATTACCAGCCATTTCACCCCCGCCCTGAAGAGTg > 1:789771/1‑63 (MQ=255) gacgTGGTTTCCGTCTGTGTCCGTCATTTATTACCAGCCATTTCACCCCCGCCCTGAAGAGTg > 1:3072828/1‑63 (MQ=255) gacgTGGTTTCCGTCTGTGTCCGTCATTTATTACCAGCCATTTCACCCCCGCCCTGAAGAGTg > 1:1367069/1‑63 (MQ=255) gacgTGGTTTCCGTCTGTGTCCGTCATTTATTACCAGCCATTTCACCCCCGCCCTGAAGAGTg > 1:46895/1‑63 (MQ=255) gacgTGGTTTCCGTCTGTGTCCGTCATTTATTACCAGCCATTTCACCCCCGCCCTGAAGAGTg > 1:3402969/1‑63 (MQ=255) gacgTGGTTTCCGTCTGTGTCCGTCATTTATTACCAGCCATTTCACCCCCGCCCTGAAGAGTg > 1:1675296/1‑63 (MQ=255) gacgTGGTTTCCGTCAGTGTCCGTCATTTATTACCAGCCATTTCACCCCCGCCCTGAAGAGTg > 1:1060515/1‑63 (MQ=255) ttCCGTCTGTGTCCGTCATTTATTACCAGCCAATTCACCCCCGCCCTGAAGAGTGAACTGa < 1:2389294/61‑1 (MQ=255) ttCCGTCTGTGTCCGTCATTTATTACCAGCCAATTCACCCCCGCCCTGAAGAGTGAACTGa < 1:264696/61‑1 (MQ=255) gTCTGTGTCCGTCATTTATTACCAGCCAATTCACCCCCGCCCTGAAGAGTg < 1:937717/51‑1 (MQ=255) cGTCATTTATTACCAGCCAATTCACGCCCGCCCTg > 1:2762422/1‑35 (MQ=255) cGTCATTTATTACCAGCCAATTCACCCCCGCCCTg > 1:2923333/1‑35 (MQ=255) cGTCATTTATTACCAGCCAATTCACCCCCGCCCTg > 1:2130874/1‑35 (MQ=255) cGTCATTTATTACCAGCCAATTCACCCCCGCCCTg > 1:1363345/1‑35 (MQ=255) cGTCATTTATTACCAGCCAATTCACCCCCGCCCTg > 1:2492325/1‑35 (MQ=255) cGTCATTTATTACCAGCCAATTCACCCCCGCCCTg > 1:2700393/1‑35 (MQ=255) cGTCATTTATTACCAGCCAATTCACCCCCGCCCTg > 1:2768480/1‑35 (MQ=255) cGTCATTTATTACCAGCCAATTCACCCCCGCCCTGAAGAGTGAACTGAAGCGGCGCGGAATTGCGCGTa > 1:1020723/1‑69 (MQ=255) cGTCATTTATTACCAGCCAATTCACCCCCGCCCTGAAGAGTGAACTGAAGCGGCGCGGAATTGCGCGTa > 1:166586/1‑69 (MQ=255) ttattaCCAGCCAATTCACCCCCGCCCTGAAGAGTGAACTGAAGCGGCGCGGAATTGCGCGTAACtttt < 1:2790267/69‑1 (MQ=255) ttattaCCAGCCAATTCACCCCCGCCCTGAAGAGTGAACTGAAGCGGCGCGGAATTGCGCGTAACtttt < 1:2939166/69‑1 (MQ=255) ttattaCCAGCCAATTCACCCCCGCCCTGAAGAGTGAACTGAAGCGGCGCGGAATTGCGCGTAACtttt < 1:3331090/69‑1 (MQ=255) ttattaCCAGCCAATTCACCCCCGCCCTGAAGAGTGAACTGAAGCGGCGCGGAATTGCGCGTAACtttt < 1:2156127/69‑1 (MQ=255) ttattaCCAGCCAATTCACCCCCGCCCTGAAGAGTGAACTGAAGCGGCGCGGAATTGCGCGTAACtttt < 1:1451555/69‑1 (MQ=255) ttattaCCAGCCAATTCACCCCCGCCCTGAAGAGTGAACTGAAGCGGCGCGGAATTGCGCGTAACtttt < 1:513057/69‑1 (MQ=255) ccATTTCACCCCCGCCCTGAAGAGTGAACTGAAGCGGCGCGGAATTGCGCGTAACTTTTACGACGATAc < 1:2905625/69‑1 (MQ=255) ccATTTCACCCCCGCCCTGAAGAGTGAACTGAAGCGGCGCGGAATTGCGCGTAACTTTTACGACGATAc < 1:1046712/69‑1 (MQ=255) ccATTTCACCCCCGCCCTGAAGAGTGAACTGAAGCGGCGCGGAATTGCGCGTAACTTTTACGACGATAc < 1:101917/69‑1 (MQ=255) ccATTTCACCCCCGCCCTGAAGAGTGAACTGAAGCGGCGCGGAATTGCGCGTAACTTTTACGACGATAc < 1:1449208/69‑1 (MQ=255) ccATTTCACCCCCGCCCTGAAGAGTGAACTGAAGCGGCGCGGAATTGCGCGTAACTTTTACGACGATAc < 1:746743/69‑1 (MQ=255) ccATTTCACCCCCGCCCTGAAGAGTGAACTGAAGCGGCGCGGAATTGCGCGTAACTTTTACGACGATAc < 1:1533692/69‑1 (MQ=255) ccATTTCACCCCCGCCCTGAAGAGTGAACTGAAGCGGCGCGGAATTGCGCGTAACTTTTACGACGATAc < 1:571027/69‑1 (MQ=255) ccATTTCACCCCCGCCCTGAAGAGTGAACTGAAGCGGCGCGGAATTGCGCGTAACTTTTACGACGATAc < 1:1571374/69‑1 (MQ=255) ccATTTCACCCCCGCCCTGAAGAGTGAACTGAAGCGGCGCGGAATTGCGCGTAACTTTTACGACGATAc < 1:488035/69‑1 (MQ=255) ccATTTCACCCCCGCCCTGAAGAGTGAACTGAAGCGGCGCGGAATTGCGCGTAACTTTTACGACGATAc < 1:1597346/69‑1 (MQ=255) ccATTTCACCCCCGCCCTGAAGAGTGAACTGAAGCGGCGCGGAATTGCGCGTAACTTTTACGACGATAc < 1:1720563/69‑1 (MQ=255) ccATTTCACCCCCGCCCTGAAGAGTGAACTGAAGCGGCGCGGAATTGCGCGTAACTTTTACGACGATAc < 1:386969/69‑1 (MQ=255) ccATTTCACCCCCGCCCTGAAGAGTGAACTGAAGCGGCGCGGAATTGCGCGTAACTTTTACGACGATAc < 1:2110231/69‑1 (MQ=255) ccATTTCACCCCCGCCCTGAAGAGTGAACTGAAGCGGCGCGGAATTGCGCGTAACTTTTACGACGATAc < 1:2179342/69‑1 (MQ=255) ccATTTCACCCCCGCCCTGAAGAGTGAACTGAAGCGGCGCGGAATTGCGCGTAACTTTTACGACGATAc < 1:2511520/69‑1 (MQ=255) ccATTTCACCCCCGCCCTGAAGAGTGAACTGAAGCGGCGCGGAATTGCGCGTAACTTTTACGACGATAc < 1:3092340/69‑1 (MQ=255) ccATTTCACCCCCGCCCTGAAGAGTGAACTGAAGCGGCGCGGAATTGCGCGTAACTTTTACGACGATAc < 1:2555523/69‑1 (MQ=255) | CAACGTTTGTGTTTATTTCACTGGCTACGACGTGGTTTCCGTCTGTGTCCGTCATTTATTACCAGCCAATTCACCCCCGCCCTGAAGAGTGAACTGAAGCGGCGCGGAATTGCGCGTAACTTTTACGACGATAC > minE/1698367‑1698500 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |