| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,698,732 | A→G | 41.7% | P204P (CCA→CCG) | csiE → | stationary phase inducible protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,698,732 | 0 | A | G | 41.7% | 9.1 / 37.0 | 54 | P204P (CCA→CCG) | csiE | stationary phase inducible protein |
| Reads supporting (aligned to +/- strand): ref base A (24/7); new base G (12/10); total (36/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.34e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.23e-05 | |||||||||||
GTAGCGCAGGAAATTGGACGCCACTGGCAGCGTCGGGCGCTCCAGCCTGTACCACCTGATGAGCCACTGTTTATGGCACTACTTTTTTCCATGTTGCGGGTTCCCGATCCATTGCGGGAT > minE/1698679‑1698798 | gTAGCGCAGGAAATTGGACGCCACTGGCAGCGTCGGGCGCTCCAGCCTGTACCACCTGATGAGCCACTg < 1:1049177/69‑1 (MQ=255)gTAGCGCAGGAAATTGGACGCCACTGGCAGCGTCGGGCGCTCCAGCCTGTACCACCTGATGAGCCACTg < 1:1051242/69‑1 (MQ=255)gTAGCGCAGGAAATTGGACGCCACTGGCAGCGTCGGGCGCTCCAGCCTGTACCACCTGATGAGCCACTg < 1:1368505/69‑1 (MQ=255)gTAGCGCAGGAAATTGGACGCCACTGGCAGCGTCGGGCGCTCCAGCCTGTACCACCTGATGAGCCACTg < 1:476708/69‑1 (MQ=255)gTAGCGCAGGAAATTGGACGCCACTGGCAGCGTCGGGCGCTCCAGCCTGTACCACCTGATGAGCCACTg < 1:687914/69‑1 (MQ=255) aGCGCAGGAAATTGGACGCCACTGGCAGCGTCGGGCGCTCCAGCCTGTACCACCTGATGAGCCACTGtt > 1:2749013/1‑69 (MQ=255) aGCGCAGGAAATTGGACGCCACTGGCAGCGTCGGGCGCTCCAGCCTGTACCACCTGATGAGCCACTGtt > 1:2618730/1‑69 (MQ=255) aGCGCAGGAAATTGGACGCCACTGGCAGCGTCGGGCGCTCCAGCCTGTACCACCTGATGAGCCACTGtt > 1:3135254/1‑69 (MQ=255) ggAAATTGGACGCCACTGGCAGCGTCGGGCGCTCCAGCCTGTACCACCTGATGAGCCACTg < 1:2306651/61‑1 (MQ=255) aaTTGGACGCCACTGGCAGCGTCGGGCGCTCCCGCCTGTACCGCCTGATGAGCCACTGTTTATGGCatt > 1:777388/1‑67 (MQ=255) aaTTGGACGCCACTGGCAGCGTCGGGCGCTCCAGCCTGTACCGCCTGATGAGCCACTGTTTATGGCatt > 1:3238369/1‑67 (MQ=255) aaTTGGACGCCACTGGCAGCGTCGGGCGCTCCAGCCTGTACCGCCTGATGAGCCACTGTTTATGGCatt > 1:3267100/1‑67 (MQ=255) aaTTGGACGCCACTGGCAGCGTCGGGCGCTCCAGCCTGTACCGCCTGATGAGCCACTGTTTATGGCatt > 1:1882834/1‑67 (MQ=255) aaTTGGACGCCACTGGCAGCGTCGGGCGCTCCAGCCTGTACCGCCTGATGAGCCACTGTTTATGGCatt > 1:42679/1‑67 (MQ=255) aaTTGGACGCCACTGGCAGCGTCGGGCGCTCCAGCCTGTACCGCCTGATGAGCCACTGTTTATGGCatt > 1:1679503/1‑67 (MQ=255) aaTTGGACGCCACTGGCAGCGTCGGGCGCTCCAGCCTGTACCGCCTGATGAGCCACTGTTTATGGCatt > 1:1423126/1‑67 (MQ=255) aaTTGGACGCCACTGGCAGCGTCGGGCGCTCCAGCCTGTACCGCCTGATGAGCCACTGTTTATGGCatt > 1:1388438/1‑67 (MQ=255) aaTTGGACGCCACTGGCAGCGTCGGGCGCTCCAGCCTGTACCGCCTGATGAGCCACTGTTTATGGCatt > 1:2689003/1‑67 (MQ=255) aaTTGGACGCCACTGGCAGCGTCGGGCGCTCCAGCCTGTACCGCCTGATGAGCCACTGTTTATGGCatt > 1:1122195/1‑67 (MQ=255) aaTTGGACGCCACTGGCAGCGTCGGGCGCTCCAGCCTGTACCGCCTGATGAGCCACTGTTTATGGCatt > 1:813483/1‑67 (MQ=255) aaTTGGACGCCACTGGCAGCGTCGGGCGCTCCAGCCTGTACCGCCTGATGAGCCACTGTTTATGGCat > 1:3145432/1‑67 (MQ=255) aaTTGGACGCCACTGGCAGCGTCGGGCGCTCCAGCCTGTACCACCtgatg > 1:1217900/1‑50 (MQ=255) aaTTGGACGCCACTGGCAGCGTCGGGCGCTCCAGCCTGTACCACCTGATGAGCCACTGTTTATg > 1:249944/1‑64 (MQ=255) aaTTGGACGCCACTGGCAGCGTCGGGCGCTCCAGCCTGTACCACCTGATGAGCCACTGTTTATGGCact > 1:2483451/1‑69 (MQ=255) aaTTGGACGCCACTGGCAGCGTCGGGCGCTCCAGCCTGTACCACCTGATGAGCCACTGTTTATGGCact > 1:3011443/1‑69 (MQ=255) aaTTGGACGCCACTGGCAGCGTCGGGCGCTCCAGCCTGTACCACCTGATGAGCCACTGTTTATGGCact > 1:2322499/1‑69 (MQ=255) aaTTGGACGCCACTGGCAGCGTCGGGCGCTCCAGCCTGTACCACCTGATGAGCCACTGTTTATGGCact > 1:660090/1‑69 (MQ=255) aaTTGGACGCCACTGGCAGCGTCGGGCGCTCCAGCCTGTACCACCTGATGAGCCACTGTTTATGGCact > 1:733238/1‑69 (MQ=255) aaTTGGACGCCACTGGCAGCGTCGGGCGCTCCAGCCTGTACCACCTGATGAGCCACTGTTTATGGCact > 1:1237520/1‑69 (MQ=255) ttGGACGCCACTGGCAGCGTCGGGCGCTCCAGCCTGTACCACCTGATGAGCCACTg < 1:3382914/56‑1 (MQ=255) cGCCACTGGCAGCGTCGGGCGCTCCAGCCTGTACCGCCTGATGAGCCACTGTTTATGGCATTACtttt < 1:3289979/68‑1 (MQ=255) cGCCACTGGCAGCGTCGGGCGCTCCAGCCTGTACCGCCTGATGAGCCACTGTTTATGGCATTACtttt < 1:3378408/68‑1 (MQ=255) cGCCACTGGCAGCGTCGGGCGCTCCAGCCTGTACCGCCTGATGAGCCACTGTTTATGGCATTACtttt < 1:854848/68‑1 (MQ=255) cGCCACTGGCAGCGTCGGGCGCTCCAGCCTGTACCGCCTGATGAGCCACTGTTTATGGCATTACtttt < 1:933724/68‑1 (MQ=255) cGCCACTGGCAGCGTCGGGCGCTCCAGCCTGTACCGCCTGATGAGCCACTGTTTATGGCATTACtttt < 1:2857065/68‑1 (MQ=255) cGCCACTGGCAGCGTCGGGCGCTCCAGCCTGTACCGCCTGATGAGCCACTGTTTATGGCATTACtttt < 1:284687/68‑1 (MQ=255) cGCCACTGGCAGCGTCGGGCGCTCCAGCCTGTACCGCCTGATGAGCCACTGTTTATGGCATTACtttt < 1:2432042/68‑1 (MQ=255) cGCCACTGGCAGCGTCGGGCGCTCCAGCCTGTACCGCCTGATGAGCCACTGTTTATGGCATTACtttt < 1:2299322/68‑1 (MQ=255) cGCCACTGGCAGCGTCGGGCGCTCCAGCCTGTACCGCCTGATGAGCCACTGTTTATGGCATTACtttt < 1:183187/68‑1 (MQ=255) cGCCACTGGCAGCGTCGGGCGCTCCAGCCTGTACCGCCTGATGAGCCACTGTTTATGGCATTACtttt < 1:207466/68‑1 (MQ=255) aCTGGCAGCGTCGGGCGCTCCAGCCTGTACCACCtgat > 1:2917334/1‑38 (MQ=255) aCTGGCAGCGTCGGGCGCTCCAGCCTGTACCACCTGATGAGCCACTGTTTATGGCACTACTTTTTTCCa > 1:1005066/1‑69 (MQ=255) aCTGGCAGCGTCGGGCGCTCCAGCCTGTACCACCTGATGAGCCACTGTTTATGGCACTACTTTTTTCCa > 1:1651592/1‑69 (MQ=255) aCTGGCAGCGTCGGGCGCTCCAGCCTGTACCACCTGATGAGCCACTGTTTATGGCACTACTTTTTTCCa > 1:681409/1‑69 (MQ=255) aCTGGCAGCGTCGGGCGCTCCAGCCTGTACCACCTGATGAGCCACTGTTTATGGCACTACTTTTTTCCa > 1:654374/1‑69 (MQ=255) aCTGGCAGCGTCGGGCGCTCCAGCCTGTACCACCTGATGAGCCACTGTTTATGGCACTACTTTTTTCCa > 1:521133/1‑69 (MQ=255) aCTGGCAGCGTCGGGCGCTCCAGCCTGTACCACCTGATGAGCCACTGTTTATGGCACTACTTTTTTCCa > 1:1747179/1‑69 (MQ=255) aCTGGCAGCGTCGGGCGCTCCAGCCTGTACCACCTGATGAGCCACTGTTTATGGCACTACTTTTTTCCa > 1:2183761/1‑69 (MQ=255) aCTGGCAGCGTCGGGCGCTCCAGCCTGTACCACCTGATGAGCCACTGTTTATGGCACTACTTTTTTCCa > 1:2317392/1‑69 (MQ=255) aCTGGCAGCGTCGGGCGCTCCAGCCTGTACCACCTGATGAGCCACTGTTTATGGCACTACTTTTTTCCa > 1:2499701/1‑69 (MQ=255) aCTGGCAGCGTCGGGCGCTCCAGCCTGTACCACCTGATGAGCCACTGTTTATGGCACTACTTTTTTCCa > 1:3124902/1‑69 (MQ=255) aCTGGCAGCGTCGGGCGCTCCAGCCTGTACCACCTGATGAGCCACTGTTTATGGCACTACTTTTTTCCa > 1:2904236/1‑69 (MQ=255) atGGCAGCGTCGGGCGCTCCAGCCTGTACCACCTGATGAGCCACTGTTTATGGCACTACTTTTTTCCAt > 1:3331989/2‑69 (MQ=255) ccTGTACCTCCTGATGAGCCACTGTTTATGGCATTACtttt < 1:1704320/41‑1 (MQ=38) ccaccTGATGAGCCACTGTTTATGGGACTACTTTTTTCCATGTTGCGGGTTCCCGATCCATTGCGGGAt > 1:508826/1‑69 (MQ=255) ccaccTGATGAGCCACTGTTTATGGCACTACTTTTTTCCATGTTGCGGGTTCCCGATCCATTGCg > 1:1087641/1‑65 (MQ=255) ccaccTGATGAGCCACTGTTTATGGCACTACTTTTTTCCATGTTGCGGGTTCCCGATCCATTGCGGGa > 1:103936/1‑68 (MQ=255) ccaccTGATGAGCCACTGTTTATGGCACTACTTTTTTCCATGTTGCGGGTTCCCGATCCATTGCGGGAt > 1:2120799/1‑69 (MQ=255) ccaccTGATGAGCCACTGTTTATGGCACTACTTTTTTCCATGTTGCGGGTTCCCGATCCATTGCGGGAt > 1:3054973/1‑69 (MQ=255) ccaccTGATGAGCCACTGTTTATGGCACTACTTTTTTCCATGTTGCGGGTTCCCGATCCATTGCGGGAt > 1:1338768/1‑69 (MQ=255) ccaccTGATGAGCCACTGTTTATGGCACTACTTTTTTCCATGTTGCGGGTTCCCGATCCATTGCGGGAt > 1:1047604/1‑69 (MQ=255) | GTAGCGCAGGAAATTGGACGCCACTGGCAGCGTCGGGCGCTCCAGCCTGTACCACCTGATGAGCCACTGTTTATGGCACTACTTTTTTCCATGTTGCGGGTTCCCGATCCATTGCGGGAT > minE/1698679‑1698798 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |