| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,698,757 | C→T | 39.4% | L213L (CTA→TTA) | csiE → | stationary phase inducible protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,698,757 | 0 | C | T | 39.4% | 38.6 / 48.9 | 76 | L213L (CTA→TTA) | csiE | stationary phase inducible protein |
| Reads supporting (aligned to +/- strand): ref base C (33/13); new base T (19/11); total (52/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.60e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AATTGGACGCCACTGGCAGCGTCGGGCGCTCCAGCCTGTACCACCTGATGAGCCACTGTTTATGGCACTACTTTTTTCCATGTTGCGGGTTCCCGATCCATTGCGGGATGCGCATCAGCGGGACAGACAA > minE/1698690‑1698819 | aaTTGGACGCCACTGGCAGCGTCGGGCGCTCCAGCCTGTACCACCTGATGAGCCACTGTTTATGGCact > 1:3011443/1‑69 (MQ=255)aaTTGGACGCCACTGGCAGCGTCGGGCGCTCCAGCCTGTACCACCTGATGAGCCACTGTTTATGGCact > 1:1237520/1‑69 (MQ=255)aaTTGGACGCCACTGGCAGCGTCGGGCGCTCCAGCCTGTACCACCTGATGAGCCACTGTTTATGGCact > 1:733238/1‑69 (MQ=255)aaTTGGACGCCACTGGCAGCGTCGGGCGCTCCAGCCTGTACCACCTGATGAGCCACTGTTTATGGCact > 1:2322499/1‑69 (MQ=255)aaTTGGACGCCACTGGCAGCGTCGGGCGCTCCAGCCTGTACCACCTGATGAGCCACTGTTTATGGCact > 1:660090/1‑69 (MQ=255)aaTTGGACGCCACTGGCAGCGTCGGGCGCTCCAGCCTGTACCACCTGATGAGCCACTGTTTATGGCact > 1:2483451/1‑69 (MQ=255) cGCCACTGGCAGCGTCGGGCGCTCCAGCCTGTACCGCCTGATGAGCCACTGTTTATGGCATTACtttt < 1:2432042/68‑1 (MQ=255) cGCCACTGGCAGCGTCGGGCGCTCCAGCCTGTACCGCCTGATGAGCCACTGTTTATGGCATTACtttt < 1:183187/68‑1 (MQ=255) cGCCACTGGCAGCGTCGGGCGCTCCAGCCTGTACCGCCTGATGAGCCACTGTTTATGGCATTACtttt < 1:2857065/68‑1 (MQ=255) cGCCACTGGCAGCGTCGGGCGCTCCAGCCTGTACCGCCTGATGAGCCACTGTTTATGGCATTACtttt < 1:284687/68‑1 (MQ=255) cGCCACTGGCAGCGTCGGGCGCTCCAGCCTGTACCGCCTGATGAGCCACTGTTTATGGCATTACtttt < 1:207466/68‑1 (MQ=255) cGCCACTGGCAGCGTCGGGCGCTCCAGCCTGTACCGCCTGATGAGCCACTGTTTATGGCATTACtttt < 1:3289979/68‑1 (MQ=255) cGCCACTGGCAGCGTCGGGCGCTCCAGCCTGTACCGCCTGATGAGCCACTGTTTATGGCATTACtttt < 1:3378408/68‑1 (MQ=255) cGCCACTGGCAGCGTCGGGCGCTCCAGCCTGTACCGCCTGATGAGCCACTGTTTATGGCATTACtttt < 1:854848/68‑1 (MQ=255) cGCCACTGGCAGCGTCGGGCGCTCCAGCCTGTACCGCCTGATGAGCCACTGTTTATGGCATTACtttt < 1:933724/68‑1 (MQ=255) cGCCACTGGCAGCGTCGGGCGCTCCAGCCTGTACCGCCTGATGAGCCACTGTTTATGGCATTACtttt < 1:2299322/68‑1 (MQ=255) aCTGGCAGCGTCGGGCGCTCCAGCCTGTACCACCTGATGAGCCACTGTTTATGGCACTACTTTTTTCCa > 1:2499701/1‑69 (MQ=255) aCTGGCAGCGTCGGGCGCTCCAGCCTGTACCACCTGATGAGCCACTGTTTATGGCACTACTTTTTTCCa > 1:1651592/1‑69 (MQ=255) aCTGGCAGCGTCGGGCGCTCCAGCCTGTACCACCTGATGAGCCACTGTTTATGGCACTACTTTTTTCCa > 1:2183761/1‑69 (MQ=255) aCTGGCAGCGTCGGGCGCTCCAGCCTGTACCACCTGATGAGCCACTGTTTATGGCACTACTTTTTTCCa > 1:2317392/1‑69 (MQ=255) aCTGGCAGCGTCGGGCGCTCCAGCCTGTACCACCTGATGAGCCACTGTTTATGGCACTACTTTTTTCCa > 1:2904236/1‑69 (MQ=255) aCTGGCAGCGTCGGGCGCTCCAGCCTGTACCACCTGATGAGCCACTGTTTATGGCACTACTTTTTTCCa > 1:1747179/1‑69 (MQ=255) aCTGGCAGCGTCGGGCGCTCCAGCCTGTACCACCTGATGAGCCACTGTTTATGGCACTACTTTTTTCCa > 1:3124902/1‑69 (MQ=255) aCTGGCAGCGTCGGGCGCTCCAGCCTGTACCACCTGATGAGCCACTGTTTATGGCACTACTTTTTTCCa > 1:521133/1‑69 (MQ=255) aCTGGCAGCGTCGGGCGCTCCAGCCTGTACCACCTGATGAGCCACTGTTTATGGCACTACTTTTTTCCa > 1:654374/1‑69 (MQ=255) aCTGGCAGCGTCGGGCGCTCCAGCCTGTACCACCTGATGAGCCACTGTTTATGGCACTACTTTTTTCCa > 1:681409/1‑69 (MQ=255) aCTGGCAGCGTCGGGCGCTCCAGCCTGTACCACCTGATGAGCCACTGTTTATGGCACTACTTTTTTCCa > 1:1005066/1‑69 (MQ=255) atGGCAGCGTCGGGCGCTCCAGCCTGTACCACCTGATGAGCCACTGTTTATGGCACTACTTTTTTCCAt > 1:3331989/2‑69 (MQ=255) ccTGTACCTCCTGATGAGCCACTGTTTATGGCATTACtttt < 1:1704320/41‑1 (MQ=38) ccaccTGATGAGCCACTGTTTATGGGACTACTTTTTTCCATGTTGCGGGTTCCCGATCCATTGCGGGAt > 1:508826/1‑69 (MQ=255) ccaccTGATGAGCCACTGTTTATGGCACTACTTTTTTCCATGTTGCGGGTTCCCGATCCATTGCg > 1:1087641/1‑65 (MQ=255) ccaccTGATGAGCCACTGTTTATGGCACTACTTTTTTCCATGTTGCGGGTTCCCGATCCATTGCGGGa > 1:103936/1‑68 (MQ=255) ccaccTGATGAGCCACTGTTTATGGCACTACTTTTTTCCATGTTGCGGGTTCCCGATCCATTGCGGGAt > 1:1047604/1‑69 (MQ=255) ccaccTGATGAGCCACTGTTTATGGCACTACTTTTTTCCATGTTGCGGGTTCCCGATCCATTGCGGGAt > 1:1338768/1‑69 (MQ=255) ccaccTGATGAGCCACTGTTTATGGCACTACTTTTTTCCATGTTGCGGGTTCCCGATCCATTGCGGGAt > 1:2120799/1‑69 (MQ=255) ccaccTGATGAGCCACTGTTTATGGCACTACTTTTTTCCATGTTGCGGGTTCCCGATCCATTGCGGGAt > 1:3054973/1‑69 (MQ=255) ccTGATGAGCCACTGTTTATGGCATTACTTTTTTCCATg > 1:1579385/1‑39 (MQ=255) ccTGATGAGCCACTGTTTATGGCATTACTTTTTTCCATg > 1:228960/1‑39 (MQ=255) ccTGATGAGCCACTGTTTATGGCATTACTTTTTTCCATg > 1:3247973/1‑39 (MQ=255) ccTGATGAGCCACTGTTTATGGCATTACTTTTTTCCATg > 1:3158507/1‑39 (MQ=255) ccTGATGAGCCACTGTTTATGGCATTACTTTTTTCCATg > 1:3431004/1‑39 (MQ=255) ccTGATGAGCCACTGTTTATGGCATTACTTTTTTCCATg > 1:3075347/1‑39 (MQ=255) ccTGATGAGCCACTGTTTATGGCATTACTTTTTTCCATg > 1:447560/1‑39 (MQ=255) ccTGATGAGCCACTGTTTATGGCATTACTTTTTTCCATg > 1:1551540/1‑39 (MQ=255) ccTGATGAGCCACTGTTTATGGCATTACTTTTTTCCATg > 1:1937776/1‑39 (MQ=255) ccTGATGAGCCACTGTTTATGGCATTACTTTTTTCCATg > 1:570393/1‑39 (MQ=255) ccTGATGAGCCACTGTTTATGGCATTACTTTTTTCCATg > 1:2008897/1‑39 (MQ=255) ccTGATGAGCCACTGTTTATGGCATTACTTTTTTCCATg > 1:2030510/1‑39 (MQ=255) ccTGATGAGCCACTGTTTATGGCATTACTTTTTTCCATg > 1:586194/1‑39 (MQ=255) ccTGATGAGCCACTGTTTATGGCATTACTTTTTTCCATg > 1:606187/1‑39 (MQ=255) ccTGATGAGCCACTGTTTATGGCATTACTTTTTTCCATg > 1:140758/1‑39 (MQ=255) ccTGATGAGCCACTGTTTATGGCATTACTTTTTTCCATg > 1:2502898/1‑39 (MQ=255) ccTGATGAGCCACTGTTTATGGCATTACTTTTTTCCATg > 1:2079224/1‑39 (MQ=255) ccTGATGAGCCACTGTTTATGGCATTACTTTTTTCCATg > 1:875405/1‑39 (MQ=255) ccTGATGAGCCACTGTTTATGGCATTACTTTTTTCCATg > 1:2223944/1‑39 (MQ=255) gaGCCACTGTTTATGGCACTACTTTTTTCCATGTTGCGGGTTCCCGAtg > 1:3143104/1‑48 (MQ=255) gaGCCACTGTTTATGGCACTACTTTTTTCCATGTTGCGGGTTCCCGATc > 1:70019/1‑49 (MQ=255) gaGCCACTGTTTATGGCACTACTTTTTTCCATGTTGCGGGTTCCCGATc > 1:1555274/1‑49 (MQ=255) gaGCCACTGTTTATGGCACTACTTTTTTCCATGTTGCGGGTTCCCGATc > 1:1580574/1‑49 (MQ=255) ccACTGTTTATGGCACTACTTTTTTCCATGTTGCGGGTTCCCGATCCATTGCGGGATGCGCATCAGCgg < 1:2288537/69‑1 (MQ=255) ccACTGTTTATGGCACTACTTTTTTCCATGTTGCGGGTTCCCGATCCATTGCGGGATGCGCATCAGCgg < 1:3427098/69‑1 (MQ=255) ccACTGTTTATGGCACTACTTTTTTCCATGTTGCGGGTTCCCGATCCATTGCGGGATGCGCATCAGCgg < 1:1414934/69‑1 (MQ=255) ccACTGTTTATGGCACTACTTTTTTCCATGTTGCGGGTTCCCGATCCATTGCGGGATGCGCATCAGCgg < 1:3075073/69‑1 (MQ=255) ccACTGTTTATGGCACTACTTTTTTCCATGTTGCGGGTTCCCGATCCATTGCGGGATGCGCATCAGCgg < 1:641823/69‑1 (MQ=255) ccACTGTTTATGGCACTACTTTTTTCCATGTTGCGGGTTCCCGATCCATTGCGGGATGCGCATCAGCgg < 1:2867983/69‑1 (MQ=255) cACTGTTTATGGCACTACTTTTTTCCATGTTGCGGGTTCCCGATCCATTGCGGGATGCGCATCAGCgg < 1:1407817/68‑1 (MQ=255) tttATGGCACTACTTTTTTCCATGTTGCGGGTTCCCGATCCATTGCGGGATGCGCATCAGCGGgacag > 1:2706473/1‑68 (MQ=255) tATGGCACTACTTTTTTCCATGTTGCGGGTTCCTGATCCATTGCGGGATGCGCATCAGCGGgacagaca > 1:1500030/1‑69 (MQ=255) tATGGCACTACTTTTTTCCATGTTGCGGGTTCCCGATCCATTGCGGGATGCGCAt > 1:2513667/1‑55 (MQ=255) tATGGCACTACTTTTTTCCATGTTGCGGGTTCCCGATCCATTGCGGGATGCGCATCAGCGGgacagaca < 1:526421/69‑1 (MQ=255) tATGGCACTACTTTTTTCCATGTTGCGGGTTCCCGATCCATTGCGGGATGCGCATCAGCGGgacagaca > 1:3195018/1‑69 (MQ=255) tATGGCACTACTTTTTTCCATGTTGCGGGTTCCCGATCCATTGCGGGATGCGCATCAGCGGgacagaca > 1:405417/1‑69 (MQ=255) tATGGCACTACTTTTTTCCATGTTGCGGGTTCCCGATCCATTGCGGGATGCGCATCAGCGGgacagaca > 1:1640567/1‑69 (MQ=255) tATGGCACTACTTTTTTCCATGTTGCGGGTTCCCGATCCATTGCGGGATGCGCATCAGCGGgacagaca > 1:694784/1‑69 (MQ=255) tATGGCACTACTTTTTTCCATGTTGCGGGTTCCCGATCCATTGCGGGATGCGCATCAGCGGgacagaca < 1:2142161/69‑1 (MQ=255) tATGGCACTACTTTTTTCCATGTTGCGGGTTCCCGATCCATTGCGGGATGCGCATCAGCGGgacagaca > 1:2407271/1‑69 (MQ=255) tATGGCACTACTTTTTTCCATGTTGCGGGTTCCCGATCCATTGCGGGATGCGCATCAGCGGgacagaca < 1:777441/69‑1 (MQ=255) tATGGCACTACTTTTTTCCATGTTGCGGGTTCCCGATCCATTGCGGGATGCGCATCAGCGGgacagaca < 1:112458/69‑1 (MQ=255) tATGGCACTACTTTTTTCCATGTTGCGGGTTCCCGATCCATTGCGGGATGCGCATCAGCGGgacagaca > 1:2344131/1‑69 (MQ=255) tATGGCACTACTTTTTTCCATGTTGCGGGTTCCCGATCCATTGCGGGATGCGCATCAGCGGgacagaca < 1:3402707/69‑1 (MQ=255) tATGGCACTACTTTTTTCCATGTTGCGGGTTCCCGACCCATTGCGGGATGCGCATCAGCGGgacagaca < 1:165600/69‑1 (MQ=255) tATGGCACTAC‑TTTTTCCATGTTGCGGGTTCCCGATCCATTGCGGGATGCGCATCAGCGGGACAGACaa > 1:2508102/1‑69 (MQ=255) | AATTGGACGCCACTGGCAGCGTCGGGCGCTCCAGCCTGTACCACCTGATGAGCCACTGTTTATGGCACTACTTTTTTCCATGTTGCGGGTTCCCGATCCATTGCGGGATGCGCATCAGCGGGACAGACAA > minE/1698690‑1698819 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |