| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,698,852 | T→C | 25.8% | H244H (CAT→CAC) | csiE → | stationary phase inducible protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,698,852 | 0 | T | C | 25.8% | 91.5 / 29.9 | 81 | H244H (CAT→CAC) | csiE | stationary phase inducible protein |
| Reads supporting (aligned to +/- strand): ref base T (44/16); new base C (8/13); total (52/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.19e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTAT > minE/1698799‑1698912 | gcgcATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGa < 1:2403806/69‑1 (MQ=255)gcgcATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGa < 1:1289964/69‑1 (MQ=255) aGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTc > 1:1308195/1‑69 (MQ=255) acagacaATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGttttt < 1:3394040/69‑1 (MQ=255) ttGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGaa < 1:2447079/69‑1 (MQ=255) ttGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGaa < 1:2056426/69‑1 (MQ=255) tGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAAc > 1:1988452/1‑69 (MQ=255) tATCAAGCGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTAt > 1:638692/1‑68 (MQ=255) tATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTAt > 1:2123684/1‑68 (MQ=255) tATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTAt > 1:2322650/1‑68 (MQ=255) tATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTAt > 1:2404719/1‑68 (MQ=255) tATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTAt > 1:2221963/1‑68 (MQ=255) tATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTAt > 1:2225891/1‑68 (MQ=255) tATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTAt > 1:2745348/1‑68 (MQ=255) tATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTAt > 1:2272141/1‑68 (MQ=255) tATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTAt > 1:1827207/1‑68 (MQ=255) tATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTAt > 1:179701/1‑68 (MQ=255) tATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTAt > 1:164022/1‑68 (MQ=255) tATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTAt > 1:3151441/1‑68 (MQ=255) tATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTAt > 1:1563832/1‑68 (MQ=255) tATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTAt > 1:3327814/1‑68 (MQ=255) tATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTAt > 1:488208/1‑68 (MQ=255) tATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTAt > 1:1326724/1‑68 (MQ=255) tATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTAt > 1:342130/1‑68 (MQ=255) tATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTAt > 1:3395384/1‑68 (MQ=255) tCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAAt > 1:1226191/1‑38 (MQ=255) tCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATg > 1:302266/1‑39 (MQ=255) tCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTatgatg > 1:2623810/1‑54 (MQ=255) tCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTatgatg > 1:2727930/1‑54 (MQ=255) tCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTatgatg > 1:1463928/1‑54 (MQ=255) tCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTatgatg > 1:1499034/1‑54 (MQ=255) tCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTatgatg > 1:467280/1‑54 (MQ=255) tCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTatgatg > 1:2819646/1‑54 (MQ=255) tCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTatgatg > 1:2232052/1‑54 (MQ=255) tCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTAtgtg > 1:346402/1‑69 (MQ=255) tCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTAtgtg > 1:283197/1‑69 (MQ=255) tCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTAtgtg > 1:558173/1‑69 (MQ=255) tCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTAtgtg > 1:958360/1‑69 (MQ=255) tCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTAtgtg > 1:2137232/1‑69 (MQ=255) tCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTAtgtg > 1:1272190/1‑69 (MQ=255) tCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTAtgtg > 1:1520079/1‑69 (MQ=255) cAAACGTCTGTTAAACCACTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTAtgcg > 1:1502953/1‑66 (MQ=255) cAAACGTCTGGTAAACCACTTTCGTGAGCTGGGAAATGTTCGTTTTTatg < 1:161522/50‑1 (MQ=255) cAAACGTCTGGTAAACCACTTTCGTGAGCTGGGAAATGTTCGTTTTTatg < 1:891424/50‑1 (MQ=255) cAAACGTCTGGTAAACCACTTTCGTGAGCTGGGAAATGTTCGTTTTTatg < 1:1490391/50‑1 (MQ=255) cAAACGTCTGGTAAACCACTTTCGTGAGCTGGGAAATGTTCGTTTTTatg < 1:3302381/50‑1 (MQ=255) cAAACGTCTGGTAAACCACTTTCGTGAGCTGGGAAATGTTCGTTTTTatg < 1:3215422/50‑1 (MQ=255) cAAACGTCTGGTAAACCACTTTCGTGAGCTGGGAAATGTTCGTTTTTatg < 1:1696594/50‑1 (MQ=255) cAAACGTCTGGTAAACCACTTTCGTGAGCTGGGAAATGTTCGTTTTTatg < 1:2981182/50‑1 (MQ=255) cAAACGTCTGGTAAACCACTTTCGTGAGCTGGGAAATGTTCGTTTTTatg < 1:2774439/50‑1 (MQ=255) cAAACGTCTGGTAAACCACTTTCGTGAGCTGGGAAATGTTCGTTTTTatg < 1:2752728/50‑1 (MQ=255) cAAACGTCTGGTAAACCACTTTCGTGAGCTGGGAAATGTTCGTTTTTatg < 1:1897609/50‑1 (MQ=255) cAAACGTCTGGTAAACCACTTTCGTGAGCTGGGAAATGTTCGTTTTTatg < 1:2450582/50‑1 (MQ=255) cAAACGTCTGGTAAACCACTTTCGTGAGCTGGGAAATGTTCGTTTTTatg < 1:1963626/50‑1 (MQ=255) cAAACGTCTGGTAAACCACTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTAtgcg > 1:756298/1‑66 (MQ=255) cAAACGTCTGGTAAACCACTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTAtgcg > 1:1063544/1‑66 (MQ=255) cAAACGTCTGGTAAACCACTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTAtgcg > 1:1178063/1‑66 (MQ=255) cAAACGTCTGGTAAACCACTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTAtgcg > 1:426465/1‑66 (MQ=255) cAAACGTCTGGTAAACCACTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTAtgcg > 1:2485641/1‑66 (MQ=255) cAAACGTCTGGTAAACCACTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTAtgcg > 1:3181379/1‑66 (MQ=255) cAAACGTCTGGTAAACCACTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTAtgcg > 1:318834/1‑66 (MQ=255) aaaCGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTAtgtg > 1:277874/1‑67 (MQ=255) aaaCGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTAtgtg > 1:844705/1‑67 (MQ=255) aaaCGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTAtgtg > 1:2263353/1‑67 (MQ=255) aaaCGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTAtgtg > 1:2770423/1‑67 (MQ=255) aaaCGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTAtgtg > 1:1956301/1‑67 (MQ=255) aCGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCa > 1:3302794/1‑69 (MQ=255) aCGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCa > 1:1862908/1‑69 (MQ=255) tGGTAAACCACTTTCTTGAGCTGGGAAATGTTCGTTTTTatg < 1:3139674/42‑1 (MQ=38) aaaCCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTAtgtg < 1:2048100/56‑1 (MQ=255) aaaCCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTAtgtg < 1:2052062/56‑1 (MQ=255) aaaCCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTAtgtg < 1:1147625/56‑1 (MQ=255) aaaCCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTAtgtg < 1:1856773/56‑1 (MQ=255) aaaCCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTAtgtg < 1:418915/56‑1 (MQ=255) aaaCCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTAtgtg < 1:1310040/56‑1 (MQ=255) aaaCCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTAtgtg < 1:1473164/56‑1 (MQ=255) aaaCCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTtat < 1:579108/67‑1 (MQ=255) aaaCCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTtat < 1:1999432/67‑1 (MQ=255) aaaCCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTta < 1:1062286/66‑1 (MQ=255) aCCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTAtgtg < 1:2958847/54‑1 (MQ=255) ccATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCtt > 1:2830975/1‑61 (MQ=255) | GCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTAT > minE/1698799‑1698912 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |