| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,699,077 | T→C | 38.9% | S319S (AGT→AGC) | csiE → | stationary phase inducible protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,699,077 | 0 | T | C | 38.9% | 25.7 / 37.3 | 59 | S319S (AGT→AGC) | csiE | stationary phase inducible protein |
| Reads supporting (aligned to +/- strand): ref base T (26/10); new base C (11/12); total (37/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.68e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01 | |||||||||||
CACCACCCGCGCGGCGCTGGCCGGATTTGAAAGTGAATACGGCGTCCATCTTTCTGATGAGGAAAGTGGTCTGGTCGCGGTGATTTTCGGTGCCTGGCTAATGCAGGAAAACGATCTGCATGAAAAACAGAT > minE/1699011‑1699142 | caccacCCGCGCGGCGCTGGCCGGATTTGAAAGTGAATACGGCGTCCATCTTTCTGATGAGGAAAGtg < 1:1188665/68‑1 (MQ=255)caccacCCGCGCGGCGCTGGCCGGATTTGAAAGTGAATACGGCGTCCATCTTTCTGATGAGGAAAGtg < 1:1109687/68‑1 (MQ=255)caccacCCGCGCGGCGCTGGCCGGATTTGAAAGTGAATACGGCGTCCATCTTTCTGATGAGGAAAGtg < 1:2843942/68‑1 (MQ=255)caccacCCGCGCGGCGCTGGCCGGATTTGAAAGTGAATACGGCGTCCATCTTTCTGATGAGGAAAGtg < 1:2359789/68‑1 (MQ=255)caccacCCGCGCGGCGCTGGCCGGATTTGAAAGTGAATACGGCGTCCATCTTTCTGATGAGGAAAGtg < 1:2199800/68‑1 (MQ=255)caccacCCGCGCGGCGCTGGCCGGATTTGAAAGTGAATACGGCGTCCATCTTTCTGATGAGGAAAGtg < 1:1351751/68‑1 (MQ=255)caccacCCGCGCGGCGCTGGCCGGATTTGAAAGTGAATACGGCGTCCATCTTTCTGATGAGGAAAGtg < 1:3111178/68‑1 (MQ=255)caccacCCGCGCGGCGCTGGCCGGATTTGAAAGTGAATACGGCGTCCATCTTTCTGATGAGGAAAGtg < 1:2052624/68‑1 (MQ=255)caccacCCGCGCGGCGCTGGCCGGATTTGAAAGTGAATACGGCGTCCATCTTTCTGATGAGGAAAGtg < 1:3270521/68‑1 (MQ=255)caccacCCGCGCGGCGCTGGCCGGATTTGAAAGTGAATACGGCGTCCATCTTTCTGATGAGGAAAGtg < 1:1694819/68‑1 (MQ=255)caccacCCGCGCGGCGCTGGCCGGATTTGAAAGTGAATACGGCGTCCATCTTTCTGATGAGGAAAGtg < 1:490956/68‑1 (MQ=255) ccaccCGCGCGGCGCTGGCCGGATTTGAAAGTGAATACGGCGTCCATCTTTCTGATGAGGAAAGtggtc > 1:2509248/1‑69 (MQ=255) accCGCGCGGCGCTGGCCGGATTTGAAAGTGAATACGGCGTCCATCTTTCTGATGAGGAAAGtggtctg > 1:298753/1‑69 (MQ=255) accCGCGCGGCGCTGGCCGGATTTGAAAGTGAATACGGCGTCCATCTTTCTGATGAGGAAAGtggtctg > 1:1932447/1‑69 (MQ=255) ggcgCTGGCCGGATTTGAAAGTGAATACGGCGTCCATCTTTCTGATGAGGAAAGTGGTCTGGTCGCGGt < 1:497981/69‑1 (MQ=255) gcgcTGGCCGGATTTGAAAGTGAATACGGCGTCCATCTTTCTGATGAGGAAAGTGGTCTGGTCGCGGTg > 1:358808/1‑69 (MQ=255) gcgcTGGCCGGATTTGAAAGTGAATACGGCGTCCATCTTTCTGATGAGGAAAGTGGTCTGGTCGCGGTg > 1:3358450/1‑69 (MQ=255) ggATTTGAAAGTGAATACGGCGTCCATCTTTCTGATGAGGAAAGTGGTCTGGTCGCGGTGATTTTCGGt < 1:516141/69‑1 (MQ=255) gATTTGAAAGTGAATACGGCGTCCATCTTTCTGATGAGGAAAGtg < 1:394747/45‑1 (MQ=255) tGAAAGTTAATACGGCGTCCATCTTTCTGATGAGGAAAGtg < 1:2266225/41‑1 (MQ=255) tGAAAGTGAATACGGCGTCCATCTTTCTGATGAGGAAAGtg < 1:3255499/41‑1 (MQ=255) tGAAAGTGAATACGGCGTCCATCTTTCTGATGAGGAAAGTGGTCTGGTCGCGGTGATTTTCGGTGCCTg < 1:930618/69‑1 (MQ=255) tGAAAGTGAATACGGCGTCCATCTTTCTGATGAGGAAAGTGGTCTGGTCGCGGTGATTTTCGGTGCCTg < 1:3224477/69‑1 (MQ=255) tGAAAGTGAATACGGCGTCCATCTTTCTGATGAGGAAAGTGGTCTGGTCGCGGTGATTTTCGGTGCCTg < 1:573378/69‑1 (MQ=255) gAAAGTGAATACGGCGTCCATCTTTCTGATGAGGAAAGTGGTCTGGTCGCGGTGATTTTCGGTGCCTg < 1:3106256/68‑1 (MQ=255) gAAAGTGAATACGGCGTCCATCTTTCTGATGAGGAAAGTGGTCTGGTCGCGGTGATTTTCGGTGCCTg < 1:3086634/68‑1 (MQ=255) aGTGAATACGGCGTCCATCTTTCTGATGAGGAAAGTGGTCTGGTCGCGGTGATTTTCGGTGCCTGGCt < 1:1686863/68‑1 (MQ=255) aCGGCGTCCATCTTTCTGATGAGGAAAGTGGTCTGGTCGCGGTGATTTTCGGTGCCTGGCTAATGCAg > 1:3007300/1‑68 (MQ=255) aCGGCGTCCATCTTTCTGATGAGGAAAGTGGTCTGGTCGCGGTGATTTTCGGTGCCTGGCTAATGCAg > 1:1338400/1‑68 (MQ=255) cGGCGTCCATCTTTCTGATGAGGAAGGTGGTCTGGTCGCGGTGATTTTCGGTGCCTGGCTAATGCAgg > 1:1474800/1‑68 (MQ=255) cGGCGTCCATCTTTCTGATGAGGAAAGTGGTCTGGTCGCGGTGATTTTCGGTGCCTGGCTAATTCAgg > 1:122002/1‑68 (MQ=255) cGGCGTCCATCTTTCTGATGAGGAAAGTGGTCTGGTCGCGGTGATTTTCGGTGCCTGGCTAATGCAgg > 1:1995739/1‑68 (MQ=255) cGGCGTCCATCTTTCTGATGAGGAAAGTGGTCTGGTCGCGGTGATTTTCGGTGCCTGGCTAATGCAgg > 1:1236708/1‑68 (MQ=255) gCGTCCATCTTTCTGATGAGGAAAGTGGTCTGGTCGCGGTGATTTTCGGTGCCTGGCTAATGCAGGaa < 1:2726659/68‑1 (MQ=255) tCCATCTTTCTGATGAGGAAAGTGGTCTGGTCGCGGTGATTTTCGGTGCCTGGCTAATGCAGGAAAAc > 1:840798/1‑68 (MQ=255) tCCATCTTTCTGATGAGGAAAGTGGTCTGGTCGCGGTGATTTTCGGTGCCTGGCTAATGCAGGAAAACg > 1:1343292/1‑69 (MQ=255) cATCTTTCTGATGAGGAAAGTGGTCTGGTCGCGGTGATTTTCGGTGCCTGGCTAATGCAGGAAAACGAt > 1:3333019/1‑69 (MQ=255) cATCTTTCTGATGAGGAAAGTGGTCTGGTCGCGGTGATTTTCGGTGCCTGGCTAATGCAGGAAAACGAt > 1:288099/1‑69 (MQ=255) cATCTTTCTGATGAGGAAAGTGGTCTGGTCGCGGTGATTTTCGGTGCCTGGCTAATGCAGGAAAACGAt > 1:445025/1‑69 (MQ=255) cATCTTTCTGATGAGGAAAGTGGTCTGGTCGCGGTGATTTTCGGTGCCTGGCTAATGCAGGAAAACGAt > 1:2185535/1‑69 (MQ=255) tCTTTCTGATGAGGAAAGTGGTCTGGTCGCGTTGATTTTCGGTGCCTGGCTAATGCAGGAAAACGATc > 1:2409344/1‑68 (MQ=255) tCTTTCTGATGAGGAAAGTGGTCTGGTCGCGGTGATTTTCGGTGCCTGGCTAATGCAGGAAAACGAt > 1:802418/1‑67 (MQ=255) tCTTTCTGATGAGGAAAGTGGTCTGGTCGCGGTGATTTTCGGTGCCTGGCTAATGCAGGAAAACGATc > 1:1485925/1‑68 (MQ=255) tCTTTCTGATGAGGAAAGTGGTCTGGTCGCGGTGATTTTCGGTGCCTGGCTAATGCAGGAAAACGATc > 1:2772686/1‑68 (MQ=255) tCTTTCTGATGAGGAAAGTGGTCTGGTCGCGGTGATTTTCGGTGCCTGGCTAATGCAGGAAAACGATc > 1:1658932/1‑68 (MQ=255) tCTTTCTGATGAGGAAAGTGGTCTGGTCGCGGTGATTTTCGGTGCCTGGCTAATGCAGGAAAACGATc > 1:1888167/1‑68 (MQ=255) tCTTTCTGATGAGGAAAGTGGTCTGGTCGCGGTGATTTTCGGTGCCTGGCTAATGCAGGAAAACGATc > 1:307662/1‑68 (MQ=255) cTTTCTGATGAGGAAAGTGGTCTGGTCGCGGTGATTTTCGGTGCCTGGCTAATGCAGGAAAACGATCTg > 1:1522807/1‑69 (MQ=255) cTTTCTGATGAGGAAAGTGGTCTGGTCGCGGTGATTTTCGGTGCCTGGCTAATGCAGGAAAACGATCTg > 1:2365187/1‑69 (MQ=255) cTTTCTGATGAGGAAAGTGGTCTGGTCGCGGTGATTTTCGGTGCCTGGCTAATGCAGGAAAACGATCTg > 1:2236664/1‑69 (MQ=255) cTTTCTGATGAGGAAAGTGGTCTGGTCGCGGTGATTTTCGGTGCCTGGCTAATGCAGGAAAACGATCTg > 1:1893693/1‑69 (MQ=255) cTTTCTGATGAGGAAAGTGGTCTGGTCGCGGTGATTTTCGGTGCCTGGCTAATGCAGGAAAACGATCTg > 1:2100424/1‑69 (MQ=255) tttCTGATGAGGAAAGCGGTCTGGTCGCGGTGATTTTTGGTGCCTGGCTAATGCAGGaa > 1:2547270/1‑59 (MQ=255) tttCTGATGAGGAAAGCGGTCTGGTCGCGGTGATTTTTGGTGCCTGGCTAATGCAGGaa > 1:774549/1‑59 (MQ=255) tttCTGATGAGGAAAGCGGTCTGGTCGCGGTGATTTTTGGTGCCTGGCTAATGCAGGaa > 1:1879509/1‑59 (MQ=255) tttCTGATGAGGAAAGCGGTCTGGTCGCGGTGATTTTTGGTGCCTGGCTAATGCAGGaa > 1:316880/1‑59 (MQ=255) tttCTGATGAGGAAAGCGGTCTGGTCGCGGTGATTTTTGGTGCCTGGCTAATGCAGGaa > 1:2519207/1‑59 (MQ=255) tttCTGATGAGGAAAGCGGTCTGGTCGCGGTGATTTTTGGTGCCTGGCTAATGCAGGaa > 1:3040980/1‑59 (MQ=255) tttCTGATGAGGAAAGCGGTCTGGTCGCGGTGATTTTTGGTGCCTGGCTAATGCAGGaa > 1:2849114/1‑59 (MQ=255) tttCTGATGAGGAAAGCGGTCTGGTCGCGGTGATTTTTGGTGCCTGGCTAATGCAGGaa > 1:2347926/1‑59 (MQ=255) tttCTGATGAGGAAAGCGGTCTGGTCGCGGTGATTTTTGGTGCCTGGCTAATGCAGGaa > 1:2903541/1‑59 (MQ=255) tttCTGATGAGGAAAGCGGTCTGGTCGCGGTGAATTTTGGt > 1:1559450/1‑41 (MQ=37) tttCTGATGAGCAAAGCGGTCTGGTCGCGGTGATTTTTGGTGCCTGGCTAATGCAGGaa > 1:2086869/1‑59 (MQ=255) tgatgaAAGTGGTCTGGTCGCGGTGATTTTCGGTGCCTg < 1:1637531/39‑1 (MQ=255) aaGCGGTCTGGTCGCGGTGATTTTTGGTGCCTGGCTAATGCAGGAAAACGACCTGCATGAGAAACAGat < 1:1870099/69‑1 (MQ=255) aaGCGGTCTGGTCGCGGTGATTTTTGGTGCCTGGCTAATGCAGGAAAACGACCTGCATGAGAAACAGat < 1:1718305/69‑1 (MQ=255) aaGCGGTCTGGTCGCGGTGATTTTTGGTGCCTGGCTAATGCAGGAAAACGACCTGCATGAGAAACAGat < 1:1700660/69‑1 (MQ=255) aaGCGGTCTGGTCGCGGTGATTTTTGGTGCCTGGCTAATGCAGGAAAACGACCTGCATGAGAAACAGat < 1:2966101/69‑1 (MQ=255) aaGCGGTCTGGTCGCGGTGATTTTTGGTGCCTGGCTAATGCAGGAAAACGACCTGCATGAGAAACAGat < 1:1581362/69‑1 (MQ=255) aaGCGGTCTGGTCGCGGTGATTTTTGGTGCCTGGCTAATGCAGGAAAACGACCTGCATGAGAAACAGat < 1:2158215/69‑1 (MQ=255) aaGCGGTCTGGTCGCGGTGATTTTTGGTGCCTGGCTAATGCAGGAAAACGACCTGCATGAGAAACAGat < 1:1523948/69‑1 (MQ=255) aaGCGGTCTGGTCGCGGTGATTTTTGGTGCCTGGCTAATGCAGGAAAACGACCTGCATGAGAAACAGat < 1:197341/69‑1 (MQ=255) aaGCGGTCTGGTCGCGGTGATTTTTGGTGCCTGGCTAATGCAGGAAAACGACCTGCATGAGAAACAGat < 1:2472251/69‑1 (MQ=255) aaGCGGTCTGGTCGCGGTGATTTTTGGTGCCTGGCTAATGCAGGAAAACGACCTGCATGAGAAACAGat < 1:2631683/69‑1 (MQ=255) aaGCGGTCTGGTCGCGGTGATTTTTGGTGCCTGGCTAATGCAGGAAAACGACCTGCATGAGAAACAGat < 1:2140985/69‑1 (MQ=255) aaGCGGTCTGGTCGCGGTGATTTTTGGTGCCTGGCTAATGCAGGAAAACGACCTGCATGAGAAACAGat < 1:948341/69‑1 (MQ=255) | CACCACCCGCGCGGCGCTGGCCGGATTTGAAAGTGAATACGGCGTCCATCTTTCTGATGAGGAAAGTGGTCTGGTCGCGGTGATTTTCGGTGCCTGGCTAATGCAGGAAAACGATCTGCATGAAAAACAGAT > minE/1699011‑1699142 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |