| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,707,042 | G→A | 15.0% | V111V (GTC→GTT) | yphB ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,707,042 | 0 | G | A | 15.0% | 161.2 / 13.7 | 92 | V111V (GTC→GTT) | yphB | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (31/47); new base A (8/6); total (39/53) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.53e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.39e-01 | |||||||||||
GACAGAGAGCGTCACCGTCAGCGTATCCGCCGTTAAATGAAACGCCTGACTTACCCGATAGTGATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGACACATTGCCACTCG > minE/1706976‑1707108 | gACAGAGAGCGTCACCGTCAGCGTATCCGCCGTTAAATGAAACGCCTGACTTACCCGATAGTGATAGac < 1:587997/69‑1 (MQ=255)gACAGAGAGCGTCACCGTCAGCGTATCCGCCGTTAAATGAAACGCCTGACTTACCCGATAGTGATAGac < 1:3226802/69‑1 (MQ=255)gACAGAGAGCGTCACCGTCAGCGTATCCGCCGTTAAATGAAACGCCTGACTTACCCGATAGTGATAGac < 1:1435136/69‑1 (MQ=255) gagCGTCACCGTCAGCGTATCCGCCGTTAAATGAAACGCCTGACTTACCCGATAGTGATAGACACCGCt > 1:1244421/1‑69 (MQ=255) cGTCACCGTCAGCGTATCCGCCGTTAAATGAAACGCCTGACTTACCCGATAGTGATAGACACCGCTGCg < 1:166454/69‑1 (MQ=255) cACCGTCAGCGTATCCGCCGTTAAATGAACCGCCTGACTTACCCGATAGTGATAGCCACCGCTGCGATg > 1:2043402/1‑69 (MQ=255) cACCGTCAGCGTATCCGCCGTTAAATGAAACGCCTGACTTACCCGATAGTGATAGACACCGCTGCGAt > 1:1524774/1‑68 (MQ=255) cACCGTCAGCGTATCCGCCGTTAAATGAAACGCCTGACTTACCCGATAGTGATAGACACCGCTGCGAt > 1:2062902/1‑68 (MQ=255) cACCGTCAGCGTATCCGCCGTTAAATGAAACGCCTGACTTACCCGATAGTGATAGACACCGCTGCGATg > 1:2730696/1‑69 (MQ=255) cACCGTCAGCGTATCCGCCGTTAAATGAAACGCCTGACTTACCCGATAGTGATAGACACCGCTGCGATg > 1:1576185/1‑69 (MQ=255) cACCGTCAGCGTATCCGCCGTTAAATGAAACGCCTGACTTACCCGATAGTGATAGACACCGCTGCGATg > 1:261765/1‑69 (MQ=255) cACCGTCAGCGTATCCGCCGTTAAATGAAACGCCTGACTTACCCGATAGTGATAGACACCGCTGCGATg > 1:2631197/1‑69 (MQ=255) cACCGTCAGCGTATCCGCCGTTAAATGAAACGCCTGACTTACCCGATAGTGATAGACACCGCTGCGATg > 1:780706/1‑69 (MQ=255) cACCGTCAGCGTATCCGCCGTTAAATGAAACGCCTGACTTACCCGATAGTGATAGACACCGCTGCGATg > 1:1018230/1‑69 (MQ=255) cGTCAGCGTATCCGCCGTTAAATGAAATGCCTGGCTTACCCGATAGTGATAAACACCGCTGAGATGtt > 1:1007881/1‑68 (MQ=255) cGTCAGCGTATCCGCCGTTAAATGAAATGCCTGGCTTACCCGATAGTGATAAACACCGCTGAGATGtt > 1:3136175/1‑68 (MQ=255) cGTCAGCGTATCCGCCGTTAAATGAAATGCCTGGCTTACCCGATAGTGATAAACACCGCTGAGATGtt > 1:245638/1‑68 (MQ=255) cGTCAGCGTATCCGCCGTTAAATGAAATGCCTGGCTTACCCGATAGTGATAAACACCGCTGAGATGtt > 1:2372764/1‑68 (MQ=255) cGTCAGCGTATCCGCCGTTAAATGAAATGCCTGGCTTACCCGATAGTGATAAACACCGCTGAGATGtt > 1:576145/1‑68 (MQ=255) cGTCAGCGTATCCGCCGTTAAATGAAATGCCTGGCTTACCCGATAGTGATAAACACCGCTGAGATGtt > 1:83775/1‑68 (MQ=255) cGTCAGCGTATCCGCCGTTAAATGAAATGCCTGGCTTACCCGATAGTGATAAACACCGCTGAGATGtt > 1:910088/1‑68 (MQ=255) cGTCAGCGTATCCGCCGTTAAATGAAATGCCTGGCTTACCCGATAGTGATAAACACCGCTGAGATGtt > 1:1469525/1‑68 (MQ=255) gTCAGCGTATCCGCCGTTAAATGAAACGCCTGACTTACCCGATAGTGATAGACACCGCTGCGATGTTCa > 1:1505206/1‑69 (MQ=255) gTCAGCGTATCCGCCGTTAAATGAAACGCCTGACTTACCCGATAGTGATAGACACCGCTGCGATGTTCa > 1:1444378/1‑69 (MQ=255) gTCAGCGTATCCGCCGTTAAATGAAACGCCTGACTTACCCGATAGTGATAGACACCGCTGCGATGTTCa > 1:2288855/1‑69 (MQ=255) gTCAGCGTATCCGCCGTTAAATGAAACGCCTGACTTACCCGATAGTGATAGACACCGCTGCGATGTTCa > 1:733795/1‑69 (MQ=255) gTCAGCGTATCCGCCGTTAAATGAAACGCCTGACTTACCCGATAGTGATAGACACCGCTGCGATGTTCa > 1:764147/1‑69 (MQ=255) gTCAGCGTATCCGCCGTTAAATGAAACGCCTGACTTACCCGATAGTGATAGACACCGCTGCGATGTTCa > 1:250157/1‑69 (MQ=255) gTCAGCGTATCCGCCGTTAAATGAAACGCCTGACTTACCCGATAGTGATAGACACCGCTGCGATGTTCa > 1:2576270/1‑69 (MQ=255) gTCAGCGTATCCGCCGTTAAATGAAACGCCTGACTTACCCGATAGTGATAGACACCGCTGCGATGTTCa > 1:2636827/1‑69 (MQ=255) gTCAGCGTATCCGCCGTTAAATGAAACGCCTGACTTACCCGATAGTGATAGACACCGCTGCGATGTTCa > 1:2942727/1‑69 (MQ=255) aTCCGCCGTTAAATGAAACGCCTGACTTACCCGATAGTGATAGac < 1:2952141/45‑1 (MQ=255) tCCGCCGTTAAATGAAACGCCTGACTTACCCGATAGTGATAGACACCGCTGCGATGTTCATACACCAGa > 1:819556/1‑69 (MQ=255) tCCGCCGTTAAATGAAACGCCTGACTTACCCGATAGTGATAGACACCGCTGCGATGTTCATACACCAGa > 1:1380583/1‑69 (MQ=255) tCCGCCGTTAAATGAAACGCCTGACTTACCCGATAGTGATAGACACCGCTGCGATGTTCATACACCAGa > 1:2457254/1‑69 (MQ=255) ccgccgTTAAATGAAACGCCTGACTTACCCGATAGTGATAGACACCGCTGCGATGTTCATACACCAGa > 1:3374497/1‑68 (MQ=255) ccgccgTTAAATGAAACGCCTGACTTACCCGATAGTGATAGACACCGCTGCGATGTTCATACACCAGa > 1:1319449/1‑68 (MQ=255) ccgccgTTAAATGAAACGCCTGACTTACCCGATAGTGATAGACACCGCTGCGATGTTCATACACCAGa > 1:247306/1‑68 (MQ=255) ccgccgTTAAATGAAACGCCTGACTTACCCGATAGTGATAGACACCGCTGCGATGTTCATACACCAGa > 1:529796/1‑68 (MQ=255) ccgccgTTAAATGAAACGCCTGACTTACCCGATAGTGATAGACACCGCTGCGATGTTCATACACCAGa > 1:2451192/1‑68 (MQ=255) ccgccgTTAAATGAAACGCCTGACTTACCCGATAGTGATAGACACCGCTGCGATGTTCATACACCAGa > 1:3365613/1‑68 (MQ=255) ccgccgTTAAATGAAACGCCTGACTTACCCGATAGTGATAGACACCGCTGCGATGTTCATACACCAGa > 1:1905363/1‑68 (MQ=255) ccgccgTTAAATGAAACGCCTGACTTACCCGATAGTGATAGACACCGCTGCGATGTTCATACACCAGa > 1:1800038/1‑68 (MQ=255) ccgccgTTAAATGAAACGCCTGACTTACCCGATAGTGATAGACACCGCTGCGATGTTCATACACCAGa > 1:91387/1‑68 (MQ=255) cgccgTTAAATGAAACGCCTGACTTACCCGATAGTGATAGACACCGCTGCGATGTTCATACACCAGac < 1:665284/68‑1 (MQ=255) cgccgTTAAATGAAACGCCTGACTTACCCGATAGTGATAGACACCGCTGCGATGTTCATACACCAGac < 1:3051966/68‑1 (MQ=255) cgccgTTAAATGAAACGCCTGACTTACCCGATAGTGATAGACACCGCTGCGATGTTCATACACCAGac < 1:33810/68‑1 (MQ=255) cgccgTTAAATGAAACGCCTGACTTACCCGATAGTGATAGACACCGCTGCGATGTTCATACACCAGac < 1:786330/68‑1 (MQ=255) ttACCCGATAGTGATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCgag < 1:3042404/68‑1 (MQ=255) ttACCCGATAGTGATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCgag < 1:1300741/68‑1 (MQ=255) ttACCCGATAGTGATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCgag < 1:3172951/68‑1 (MQ=255) ttACCCGATAGTGATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCgag < 1:1262358/68‑1 (MQ=255) ttACCCGATAGTGATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCgag < 1:46744/68‑1 (MQ=255) ttACCCGATAGTGATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCgag < 1:1209724/68‑1 (MQ=255) ttACCCGATAGTGATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCgag < 1:613446/68‑1 (MQ=255) ttACCCGATAGTGATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCgag < 1:2078093/68‑1 (MQ=255) ttACCCGATAGTGATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCgag < 1:1185779/68‑1 (MQ=255) ttACCCGATAGTGATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCgag < 1:1855154/68‑1 (MQ=255) ccGATAGTGATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGacac < 1:464605/68‑1 (MQ=255) ccGATAGTGATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGacac < 1:437108/68‑1 (MQ=255) ccGATAGTGATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGacac < 1:588679/68‑1 (MQ=255) ccGATAGTGATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGacac < 1:641449/68‑1 (MQ=255) ccGATAGTGATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGacac < 1:1139669/68‑1 (MQ=255) ccGATAGTGATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGacac < 1:1668845/68‑1 (MQ=255) ccGATAGTGATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGacac < 1:3293655/68‑1 (MQ=255) ccGATAGTGATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGacac < 1:3002865/68‑1 (MQ=255) ccGATAGTGATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGacac < 1:2335742/68‑1 (MQ=255) ccGATAGTGATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGacac < 1:2504919/68‑1 (MQ=255) cGATAGTGATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGAcaca < 1:1882088/68‑1 (MQ=255) cGATAGTGATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGAcaca < 1:700534/68‑1 (MQ=255) cGATAGTGATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGAcaca < 1:679054/68‑1 (MQ=255) cGATAGTGATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGAcaca < 1:674856/68‑1 (MQ=255) cGATAGTGATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGAcaca < 1:2525517/68‑1 (MQ=255) cGATAGTGATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGAcaca < 1:2538249/68‑1 (MQ=255) cGATAGTGATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGAcaca < 1:1836660/68‑1 (MQ=255) cGATAGTGATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGAcaca < 1:2644411/68‑1 (MQ=255) cGATAGTGATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGAcaca < 1:1003143/68‑1 (MQ=255) cGATAGTGATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGAcaca < 1:2958422/68‑1 (MQ=255) cGATAGTGATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGAcaca < 1:3078880/68‑1 (MQ=255) cGATAGTGATAGACACCGCTGCGATGTGCATAGACCAGACACAAACTATCATCGCTGTGCGAGacac < 1:2847347/67‑1 (MQ=255) cGATAGTGATAGACACCGCTGCGATCTTCATACACCAGACACAAACTATCATCGCTGTGCGAGAcaca < 1:210285/68‑1 (MQ=255) gATAGTGATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGAcaca < 1:1919366/67‑1 (MQ=255) tAGTGATAAACACCGCTGAGATGTTCATACACCAGACACAAGCTATCATCGCTGCGCGAGACACATTGc < 1:1441623/69‑1 (MQ=255) tAGTGATAAACACCGCTGAGATGTTCATACACCAGACACAAGCTATCATCGCTGCGCGAGACACATTGc < 1:507764/69‑1 (MQ=255) tAGTGATAAACACCGCTGAGATGTTCATACACCAGACACAAGCTATCATCGCTGCGCGAGACACATTGc < 1:2666356/69‑1 (MQ=255) tAGTGATAAACACCGCTGAGATGTTCATACACCAGACACAAGCTATCATCGCTGCGCGAGACACATTGc < 1:401521/69‑1 (MQ=255) tAGTGATAAACACCGCTGAGATGTTCATACACCAGACACAAGCTATCATCGCTGCGCGAGACACATTGc < 1:2231454/69‑1 (MQ=255) tAGTGATAAACACCGCTGAGATGTTCATACACCAGACACAAGCTATCATCGCTGCGCGAGACACATTGc < 1:1636476/69‑1 (MQ=255) aGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGACACATTGCCACTCg < 1:1664555/68‑1 (MQ=255) aGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGACACATTGCCACTCg < 1:1244677/68‑1 (MQ=255) aGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGACACATTGCCACTCg < 1:2865798/68‑1 (MQ=255) aGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGACACATTGCCACTCg < 1:3345398/68‑1 (MQ=255) | GACAGAGAGCGTCACCGTCAGCGTATCCGCCGTTAAATGAAACGCCTGACTTACCCGATAGTGATAGACACCGCTGCGATGTTCATACACCAGACACAAACTATCATCGCTGTGCGAGACACATTGCCACTCG > minE/1706976‑1707108 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |