| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,707,754 | G→T | 30.5% | L232M (CTG→ATG) | yphC ← | predicted oxidoreductase, Zn‑dependent and NAD(P)‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,707,754 | 0 | G | T | 30.5% | 143.7 / 64.0 | 108 | L232M (CTG→ATG) | yphC | predicted oxidoreductase, Zn‑dependent and NAD(P)‑binding |
| Reads supporting (aligned to +/- strand): ref base G (54/21); new base T (19/14); total (73/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.81e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 110 total aligned reads displayed.
TTACCGGAACAATCGAGCGCAACATCCGCGCCACCGTGGGTGAGTTCGGCGATAATCTGCGGCAGACCTTCGGTGGTTGCTAAATAGCCGTGATCCATCACCCCTAACTGTTTTGCCATCGCCAGA > minE/1707690‑1707815 | ttACCGGAACAATCGAGCGCAACATCCGCGCCACCGTGGGTGAGTTCGGCGATAATCTGCGGCAGACCt > 1:2690065/1‑69 (MQ=255)ttACCGGAACAATCGAGCGCAACATCCGCGCCACCGTGGGTGAGTTCGGCGATAATCTGCGGCAGACCt > 1:1118340/1‑69 (MQ=255)ttACCGGAACAATCGAGCGCAACATCCGCGCCACCGTGGGTGAGTTCGGCGATAATCTGCGGCAGACCt > 1:2940934/1‑69 (MQ=255) aCCGGAACAATCGAGCGCAACATCCGCGCCACCGTGGGTGAGTTCGGCGATAATCTGCGGCAg > 1:1908467/1‑63 (MQ=255) aCCGGAACAATCGAGCGCAACATCCGCGCCACCGTGGGTGAGTTCGGCGATAATCTGCGGCAg > 1:2590334/1‑63 (MQ=255) aCCGGAACAATCGAGCGCAACATCCGCGCCACCGTGGGTGAGTTCGGCGATAATCTGCGGCAGACCtt > 1:3271444/1‑68 (MQ=255) aCCGGAACAATCGAGCGCAACATCCGCGCCACCGTGGGTGAGTTCGGCGATAATCTGCGGCAGACCtt > 1:37091/1‑68 (MQ=255) aCCGGAACAATCGAGCGCAACATCCGCGCCACCGTGGGTGAGTTCGGCGATAATCTGCGGCAGACCTTc > 1:1794758/1‑69 (MQ=255) aCCGGAACAATCGAGCGCAACATCCGCGCCACCGTGGGTGAGTTCGGCGATAATCTGCGGCAGACCTTc > 1:121924/1‑69 (MQ=255) aCCGGAACAATCGAGCGCAACATCCGCGCCACCGTGGGTGAGTTCGGCGATAATCTGCGGCAGACCTTc > 1:3318059/1‑69 (MQ=255) aCCGGAACAATCGAGCGCAACATCCGCGCCACCGTGGGTGAGTTCGGCGATAATCTGCGGCAGACCTTc > 1:2169688/1‑69 (MQ=255) aCCGGAACAATCGAGCGCAACATCCGCGCCACCGTGGGTGAGTTCGGCGATAATCTGCGGCAGACCTTc > 1:3270190/1‑69 (MQ=255) aCCGGAACAATCGAGCGCAACATCCGCGCCACCGTGGGTGAGTTCGGCGATAATCTGCGGCAGACCTTc > 1:2447519/1‑69 (MQ=255) aCCGGAACAATCGAGCGCAACATCCGCGCCACCGTGGGTGAGTTCGGCGATAATCTGCGGCAGACCTTc > 1:1740786/1‑69 (MQ=255) aCCGGAACAATCGAGCGCAACATCCGCGCCACCGTGGGTGAGTTCGGCGATAATCTGCGGCAGACCGTc > 1:2724083/1‑69 (MQ=255) aCCGGAACAATCGAGCGCAACATCCGCGCCACCGTGGGTGAGTT‑GGCGATAATCTGCGGCAGACCTTCg > 1:820653/1‑69 (MQ=255) gAACAATCGAGCGCAACATCCGCGCCACCGTGGGTGAGTTCGGCGATAATCTGCGGCAGACCTTCggtg < 1:391014/69‑1 (MQ=255) gAACAATCGAGCGCAACATCCGCGCCACCGTGGGTGAGTTCGGCGATAATCTGCGGCAGACCTTCggtg < 1:1789566/69‑1 (MQ=255) gAACAATCGAGCGCAACATCCGCGCCACCGTGGGTGAGTTCGGCGATAATCTGCGGCAGACCTTCggtg < 1:543840/69‑1 (MQ=255) gAACAATCGAGCGCAACATCCGCGCCACCGTGGGTGAGTTCGGCGATAATCTGCGGCAGACCTTCggtg < 1:1773926/69‑1 (MQ=255) gAACAATCGAGCGCAACATCCGCGCCACCGTGGGTGAGTTCGGCGATAATCTGCGGCAGACCTTCggtg < 1:2526465/69‑1 (MQ=255) gAACAATCGAGCGCAACATCCGCGCCACCGTGGGTGAGTTCGGCGATAATCTGCGGCAGACCTTCggtg < 1:734826/69‑1 (MQ=255) caaGCGCAACATCCGCGCCGCCGTGAGTGAGTTCGGCGATTATCTGCGGCATACCTTCggtgg > 1:1051525/3‑63 (MQ=255) caaGCGCAACATCCGCGCCGCCGTGAGTGAGTTCGGCGATTATCTGCGGCATACCTTCggtgg > 1:2008805/3‑63 (MQ=255) caaGCGCAACATCCGCGCCGCCGTGAGTGAGTTCGGCGATTATCTGCGGCATACCTTCggtgg > 1:2946610/3‑63 (MQ=255) caaGCGCAACATCCGCGCCGCCGTGAGTGAGTTCGGCGATTATCTGCGGCATACCTTCggtgg > 1:2347698/3‑63 (MQ=255) caaGCGCAACATCCGCGCCGCCGTGAGTGAGTTCGGCGATTATCTGCGGCATACCTTCggtgg > 1:237514/3‑63 (MQ=255) caaGCGCAACATCCGCGCCGCCGTGAGTGAGTTCGGCGATTATCTGCGGCATACCTTCggtgg > 1:3105794/3‑63 (MQ=255) caaGCGCAACATCCGCGCCGCCGTGAGTGAGTTCGGCGATTATCTGCGGCATACCTTCggtgg > 1:2897544/3‑63 (MQ=255) caaGCGCAACATCCGCGCCGCCGTGAGTGAGTTCGGCGATTATCTGCGGCATACCTTCggtgg > 1:1388255/3‑63 (MQ=255) caaGCGCAACATCCGCGCCGCCGTGAGTGAGTTCGGCGATTATCTGCGGCATACCTTCggtgg > 1:1960732/3‑63 (MQ=255) caaGCGCAACATCCGCGCCGCCGTGAGTGAGTTCGGCGATTATCTGCGGCATACCTTCggtgg > 1:1960940/3‑63 (MQ=255) caaGCGCAACATCCGCGCCGCCGTGAGTGAGTTCGGCGATTATCTGCGGCATACCTTCggtgg > 1:1131184/3‑63 (MQ=255) caaGCGCAACATCCGCGCCGCCGTGAGTGAGTTCGGCGATTATCTGCGGCATACCTTCggtgg > 1:1073326/3‑63 (MQ=255) caaGCGCAACATCCGCGCCGCCGTGAGTGAGTTCGGCGATTATCTGCGGCATACCTTCggtgg > 1:1548173/3‑63 (MQ=255) caaGCGCAACATCCGCGCCGCCGTGAGTGAGTTCGGCGATTATCTGCGGCATACCTTCggtgg > 1:690550/3‑63 (MQ=255) caaGCGCAACATCCGCGCCGCCGTGAGTGAGTTCGGCGATTATCTGCGGCATACCTTCggtg > 1:3431261/3‑62 (MQ=255) caaGCGCAACATCCGCGCCGCCGTGAGTGAGGTCGGCGATTATCTGCGGCATACCTTCggtg > 1:2716936/3‑62 (MQ=255) gcgcAACATCCGCGCCACCGTGGGTGAGTTCGGCGATAATCTGCGGCAGACCTTCGGTGGTTGCTaaa < 1:2055126/68‑1 (MQ=255) gcgcAACATCCGCGCCACCGTGGGTGAGTTCGGCGATAATCTGCGGCAGACCTTCGGTGGTTGCTaaa < 1:2546925/68‑1 (MQ=255) gcgcAACATCCGCGCCACCGTGGGTGAGTTCGGCGATAATCTGCGGCAGACCTTCGGTGGTTGCTaaa < 1:151100/68‑1 (MQ=255) gcgcAACATCCGCGCCACCGTGGGTGAGTTCGGCGATAATCTGCGGCAGACCTTCGGTGGTTGCTAAAt < 1:2303063/69‑1 (MQ=255) gcgcAACATCCGCGCCACCGTGGGTGAGTTCGGCGATAATCTGCGGCAGACCTTCGGTGGTTGCTAAAt < 1:2681116/69‑1 (MQ=255) gcgcAACATCCGCGCCACCGTGGGTGAGTTCGGCGATAATCTGCGGCAGACCTTCGGTGGTTGCTAAAt < 1:1347104/69‑1 (MQ=255) gcgcAACATCCGCGCCACCGTGGGTGAGTTCGGCGATAATCTGCGGCAGACCTTCGGTGGTTGCTAAAt < 1:2492860/69‑1 (MQ=255) gcgcAACATCCGCGCCACCGTGGGTGAGTTCGGCGATAATCTGCGGCAGACCTTCGGTGGTTGCTAAAt < 1:128766/69‑1 (MQ=255) gcgcAACATCCGCGCCACCGTGGGTGAGTTCGGCGATAATCTGCGGCAGACCTTCGGTGGTTGCTAAAt < 1:1850517/69‑1 (MQ=255) gcgcAACATCCGCGCCACCGTGGGTGAGTTCGGCGATAATCTGCGGCAGACCTTCGGTGGTTGCTAAAt < 1:2096757/69‑1 (MQ=255) cgcAACATCCGCGCCACCGTGGGTGAGTTCGGCGATAATCTGCGGCAGACCTTCGGTGGTTGCTAAATa < 1:2014059/69‑1 (MQ=255) aCATCCGCGCCACCGTGGGTGAGTTCGGCGATAATCTGCGGCAGACCTTCGGTGGTTGCTAAATAGcc > 1:236898/1‑68 (MQ=255) aCATCCGCGCCACCGTGGGTGAGTTCGGCGATAATCTGCGGCAGACCTTCGGTGGTTGCTAAATAGcc > 1:3244251/1‑68 (MQ=255) aCATCCGCGCCACCGTGGGTGAGTTCGGCGATAATCTGCGGCAGACCTTCGGTGGTTGCTAAATAGcc > 1:5085/1‑68 (MQ=255) aCATCCGCGCCACCGTGGGTGAGTTCGGCGATAATCTGCGGCAGACCTTCGGTGGTTGCTAAATAGcc > 1:1103702/1‑68 (MQ=255) aCATCCGCGCCACCGTGGGTGAGTTCGGCGATAATCTGCGGCAGACCTTCGGTGGTTGCTAAATAGcc > 1:2187954/1‑68 (MQ=255) aCATCCGCGCCACCGTGGGTGAGTTCGGCGATAATCTGCGGCAGACCTTCGGTGGTTGCTAAATAGcc > 1:2049212/1‑68 (MQ=255) aCATCCGCGCCACCGTGGGTGAGTTCGGCGATAATCTGCGGCAGACCTTCGGTGGTTGCTAAATAGc > 1:2853928/1‑67 (MQ=255) tCCGCGCCACCGTGGGTGAGTTCGGCGATAATCTGCGGCAGACCTTCGGTGGTTGCTAAATAGCCGTGa > 1:2411712/1‑69 (MQ=255) tCCGCGCCACCGTGGGTGAGTTCGGCGATAATCTGCGGCAGACCTTCGGTGGTTGCTAAATAGCCGTGa > 1:928715/1‑69 (MQ=255) tCCGCGCCACCGTGGGTGAGTTCGGCGATAATCTGCGGCAGACCTTCGGTGGTTGCTAAATAGCCGTGa > 1:3247519/1‑69 (MQ=255) tCCGCGCCACCGTGGGTGAGTTCGGCGATAATCTGCGGCAGACCTTCGGTGGTTGCTAAATAGCCGTGa > 1:902225/1‑69 (MQ=255) cgcgCCACCGTGGGTGAGTTCGGCGATAATCTGCGGCAGACCTTCGGTGGTTGCTAAAt < 1:593464/59‑1 (MQ=255) cgcgCCACCGTGGGTGAGTTCGGCGATAATCTGCGGCAGACCTTCGGTGGTTGCTAAATAGCCGTGAt > 1:3278225/1‑68 (MQ=255) cgcgCCACCGTGGGTGAGTTCGGCGATAATCTGCGGCAGACCTTCGGTGGTTGCTAAATAGCCGTGATc > 1:338665/1‑69 (MQ=255) cgcgCCACCGTGGGTGAGTTCGGCGATAATCTGCGGCAGACCTTCGGTGGTTGCTAAATAGCCGTGATc > 1:1171043/1‑69 (MQ=255) cgcgCCACCGTGGGTGAGTTCGGCGATAATCTGCGGCAGACCTTCGGTGGTTGCTAAATAGCCGTGATc < 1:660125/69‑1 (MQ=255) cgcgCCACCGTGGGTGAGTTCGGCGATAATCTGCGGCAGACCTTCGGTGGTTGCTAAATAGCCGTGATc < 1:2536160/69‑1 (MQ=255) cgcgCCACCGTGGGTGAGTTCGGCGATAATCTGCGGCAGACCTTCGGTGGTTGCTAAATAGCCGTGATc > 1:3043152/1‑69 (MQ=255) cgcgCCACCGTGGGTGAGTTCGGCGATAATCTGCGGCAGACCTTCGGTGGTTGCTAAATAGCCGTGATc > 1:3304957/1‑69 (MQ=255) cgcgCCACCGTGGGTGAGTTCGGCGATAATCTGCGGCAGACCTTCGGTGGTTGCTAAATAGCCGTGATc > 1:1776795/1‑69 (MQ=255) cgcgCCACCGTGGGTGAGTTCGGCGATAATCTGCGGCAGACCTTCGGTGGTTGCTAAATAGCCGTGATc < 1:2260337/69‑1 (MQ=255) cgcgCCACCGTGGGTGAGTTCGGCGATAATCTGCGGCAGACCTTCGGTGGTTGCTAAATAGCCGTGATc > 1:547943/1‑69 (MQ=255) gATTATCTGCGGCATACCTTCGGTGGTCGCTAAATAGCCGTGATCCATCACTCCTAAc < 1:342955/58‑1 (MQ=255) gATTATCTGCGGCATACCTTCGGTGGTCGCTAAATAGCCGTGATCCATCACTCCTAAc < 1:3358285/58‑1 (MQ=255) gATTATCTGCGGCATACCTTCGGTGGTCGCTAAATAGCCGTGATCCATCACTCCTAAc < 1:65611/58‑1 (MQ=255) gATTATCTGCGGCATACCTTCGGTGGTCGCTAAATAGCCGTGATCCATCACTCCTAAc < 1:39224/58‑1 (MQ=255) gATTATCTGCGGCATACCTTCGGTGGTCGCTAAATAGCCGTGATCCATCACTCCTAAc < 1:1071798/58‑1 (MQ=255) gATTATCTGCGGCATACCTTCGGTGGTCGCTAAATAGCCGTGATCCATCACTCCTAAc < 1:1253119/58‑1 (MQ=255) gATTATCTGCGGCATACCTTCGGTGGTCGCTAAATAGCCGTGATCCATCACTCCTAAc < 1:2850789/58‑1 (MQ=255) gATTATCTGCGGCATACCTTCGGTGGTCGCTAAATAGCCGTGATCCATCACTCCTAAc < 1:2744726/58‑1 (MQ=255) gATTATCTGCGGCATACCTTCGGTGGTCGCTAAATAGCCGTGATCCATCACTCCTAAc < 1:223039/58‑1 (MQ=255) gATTATCTGCGGCATACCTTCGGTGGTCGCTAAATAGCCGTGATCCATCACTCCTAAc < 1:2228019/58‑1 (MQ=255) gATTATCTGCGGCATACCTTCGGTGGTCGCTAAATAGCCGTGATCCATCACTCCTAAc < 1:2175472/58‑1 (MQ=255) gATTATCTGCGGCATACCTTCGGTGGTCGCTAAATAGCCGTGATCCATCACTCCTAAc < 1:2068171/58‑1 (MQ=255) gATTATCTGCGGCATACCTTCGGTGGTCGCTAAATAGCCGTGATCCATCACACCTAAc < 1:3146532/58‑1 (MQ=255) gATTATCTGCGGCATACCTTCGGTGGGCGCTAAATAGCCGTGATCCATCACTCCTAAc < 1:1177076/58‑1 (MQ=255) aTCTGCGGCAGACCTTCGGTGGTTGCTAAATAGCCGt > 1:860137/1‑37 (MQ=255) aTCTGCGGCAGACCTTCGGTGGTTGCTAAATAGCCGTg > 1:2265367/1‑38 (MQ=255) aTCTGCGGCAGACCTTCGGTGGTTGCTAAATAGCCGTGCTCCATCACCCCTAACTGTTTTGCCATc > 1:2577761/1‑66 (MQ=255) aTCTGCGGCAGACCTTCGGTGGTTGCTAAATAGCCGTGATCCATCACCCCTAACTGTTTTGCCATc > 1:3017251/1‑66 (MQ=255) aTCTGCGGCAGACCTTCGGTGGTTGCTAAATAGCCGTGATCCATCACCCCTAACTGTTTTGCCATc > 1:1427105/1‑66 (MQ=255) aTCTGCGGCAGACCTTCGGTGGTTGCTAAATAGCCGTGATCCATCACCCCTAACTGTTTTGCCATc > 1:1603709/1‑66 (MQ=255) aTCTGCGGCAGACCTTCGGTGGTTGCTAAATAGCCGTGATCCATCACCCCTAACTGTTTTGCCATc > 1:3349101/1‑66 (MQ=255) cTGCGGCAGACCTTCGGTGGTTGCTAAATAGCCGTGATCCATCACCCCTAACTGTTTTGCCATCGCCAg > 1:970228/1‑69 (MQ=255) tGCGGCAGACCTTCGGTGGTTGTTAAATAGCCGTGATCCATCACCCCTAACTGTTTTGCCATCGCCAGa > 1:1283441/1‑69 (MQ=255) tGCGGCAGACCTTCGGTGGTTGCTAAATAGCCGTGATCCATCACCCCTAACTGTTTTGCCATCGCCAGa > 1:2611673/1‑69 (MQ=255) tGCGGCAGACCTTCGGTGGTTGCTAAATAGCCGTGATCCATCACCCCTAACTGTTTTGCCATCGCCAGa > 1:1319399/1‑69 (MQ=255) tGCGGCAGACCTTCGGTGGTTGCTAAATAGCCGTGATCCATCACCCCTAACTGTTTTGCCATCGCCAGa > 1:2904292/1‑69 (MQ=255) tGCGGCAGACCTTCGGTGGTTGCTAAATAGCCGTGATCCATCACCCCTAACTGTTTTGCCATCGCCAGa > 1:824396/1‑69 (MQ=255) tGCGGCAGACCTTCGGTGGTTGCTAAATAGCCGTGATCCATCACCCCTAACTGTTTTGCCATCGCCAGa > 1:2905571/1‑69 (MQ=255) tGCGGCAGACCTTCGGTGGTTGCTAAATAGCCGTGATCCATCACCCCTAACTGTTTTGCCATCGCCAGa > 1:1076030/1‑69 (MQ=255) | TTACCGGAACAATCGAGCGCAACATCCGCGCCACCGTGGGTGAGTTCGGCGATAATCTGCGGCAGACCTTCGGTGGTTGCTAAATAGCCGTGATCCATCACCCCTAACTGTTTTGCCATCGCCAGA > minE/1707690‑1707815 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |