| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,709,926 | C→T | 58.0% | V374V (GTG→GTA) | yphE ← | fused predicted sugar transporter subunits and ATP‑binding components of ABC superfamily |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,709,926 | 0 | C | T | 58.0% | 12.1 / 35.2 | 50 | V374V (GTG→GTA) | yphE | fused predicted sugar transporter subunits and ATP‑binding components of ABC superfamily |
| Reads supporting (aligned to +/- strand): ref base C (10/11); new base T (4/25); total (14/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.20e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.91e-01 | |||||||||||
GGCGGCCTTGACCGTCATCCGCTGCATCACCTCTTCGGTCAGGCGGCGGATGGTGGACCATTGCAGCACACCGTTGGCGCTGATTTTTTGCCGATTGGTCAGCACTGTATTTTCGTCAACGCCCAACCAG > minE/1709860‑1709989 | ggcggcCTTGACCGTCATCCGCTGCATCACCTCTTCGGTCAGGCGGCGGATGGTGGACCATTGCAGcac > 1:2918925/1‑69 (MQ=255)ggcggcCTTGACCGTCATCCGCTGCATCACCTCTTCGGTCAGGCGGCGGATGGTGGACCATTGCAGcac > 1:2100635/1‑69 (MQ=255) cGCTGCATCACCTCTTCGGTCAGGCGGCGGATGGTGGACCATTGCAGCACACCGTTGGCGCTGAttttt > 1:357541/1‑69 (MQ=255) cGCTGCATCACCTCTTCGGTCAGGCGGCGGATGGTGGACCATTGCAGCACACCGTTGGCGCTGAttttt > 1:51427/1‑69 (MQ=255) tCAGGCGGCGGATGGTGGACCATTGCAGCACACCGTTGGCGCTGATTTTTTGCCGATTGGTCAGCACTg < 1:3426528/69‑1 (MQ=255) tCAGGCGGCGGATGGTGGACCATTGCAGCACACCGTTGGCGCTGATTTTTTGCCGATTGGTCAGCACTg < 1:686193/69‑1 (MQ=255) tCAGGCGGCGGATGGTGGACCATTGCAGCACACCGTTGGCGCTGATTTTTTGCCGATTGGTCAGCACTg < 1:178102/69‑1 (MQ=255) gcAGGCGGCGGATGGTGGACCATTGCAGCACACCGTTGGCGCTGATTTTTTGCCGATTGGTCAGCACTg < 1:2742563/68‑1 (MQ=255) gcggATGGTGGACCATTGCAGTACACCGTTGGCGCTGATTTTTTGCCGATTGGTCAGCTCTGTATTTTc < 1:2746740/69‑1 (MQ=255) gcggATGGTGGACCATTGCAGTACACCGTTGGCGCTGATTTTTTGCCGATTGGTCAGCACTGTATTTTc < 1:546850/69‑1 (MQ=255) gcggATGGTGGACCATTGCAGTACACCGTTGGCGCTGATTTTTTGCCGATTGGTCAGCACTGTATTTTc < 1:569935/69‑1 (MQ=255) gcggATGGTGGACCATTGCAGTACACCGTTGGCGCTGATTTTTTGCCGATTGGTCAGCACTGTATTTTc < 1:387618/69‑1 (MQ=255) gcggATGGTGGACCATTGCAGTACACCGTTGGCGCTGATTTTTTGCCGATTGGTCAGCACTGTATTTTc < 1:667649/69‑1 (MQ=255) gcggATGGTGGACCATTGCAGTACACCGTTGGCGCTGATTTTTTGCCGATTGGTCAGCACTGTATTTTc < 1:68513/69‑1 (MQ=255) gcggATGGTGGACCATTGCAGTACACCGTTGGCGCTGATTTTTTGCCGATTGGTCAGCACTGTATTTTc < 1:848997/69‑1 (MQ=255) gcggATGGTGGACCATTGCAGTACACCGTTGGCGCTGATTTTTTGCCGATTGGTCAGCACTGTATTTTc < 1:3402144/69‑1 (MQ=255) gcggATGGTGGACCATTGCAGTACACCGTTGGCGCTGATTTTTTGCCGATTGGTCAGCACTGTATTTTc < 1:3360576/69‑1 (MQ=255) gcggATGGTGGACCATTGCAGTACACCGTTGGCGCTGATTTTTTGCCGATTGGTCAGCACTGTATTTTc < 1:3282732/69‑1 (MQ=255) gcggATGGTGGACCATTGCAGTACACCGTTGGCGCTGATTTTTTGCCGATTGGTCAGCACTGTATTTTc < 1:857140/69‑1 (MQ=255) gcggATGGTGGACCATTGCAGTACACCGTTGGCGCTGATTTTTTGCCGATTGGTCAGCACTGTATTTTc < 1:3127525/69‑1 (MQ=255) gcggATGGTGGACCATTGCAGTACACCGTTGGCGCTGATTTTTTGCCGATTGGTCAGCACTGTATTTTc < 1:906712/69‑1 (MQ=255) gcggATGGTGGACCATTGCAGTACACCGTTGGCGCTGATTTTTTGCCGATTGGTCAGCACTGTATTTTc < 1:106787/69‑1 (MQ=255) gcggATGGTGGACCATTGCAGTACACCGTTGGCGCTGATTTTTTGCCGATTGGTCAGCACTGTATTTTc < 1:1547169/69‑1 (MQ=255) gcggATGGTGGACCATTGCAGTACACCGTTGGCGCTGATTTTTTGCCGATTGGTCAGCACTGTATTTTc < 1:1403462/69‑1 (MQ=255) gcggATGGTGGACCATTGCAGTACACCGTTGGCGCTGATTTTTTGCCGATTGGTCAGCACTGTATTTTc < 1:1567765/69‑1 (MQ=255) gcggATGGTGGACCATTGCAGTACACCGTTGGCGCTGATTTTTTGCCGATTGGTCAGCACTGTATTTTc < 1:1026898/69‑1 (MQ=255) gcggATGGTGGACCATTGCAGTACACCGTTGGCGCTGATTTTTTGCCGATTGGTCAGCACTGTATTTTc < 1:1617201/69‑1 (MQ=255) gcggATGGTGGACCATTGCAGTACACCGTTGGCGCTGATTTTTTGCCGATTGGTCAGCACTGTATTTTc < 1:1738129/69‑1 (MQ=255) gcggATGGTGGACCATTGCAGTACACCGTTGGCGCTGATTTTTTGCCGATTGGTCAGCACTGTATTTTc < 1:215548/69‑1 (MQ=255) gcggATGGTGGACCATTGCAGTACACCGTTGGCGCTGATTTTTTGCCGATTGGTCAGCACTGTATTTTc < 1:2622356/69‑1 (MQ=255) gcggATGGTGGACCATTGCAGTACACCGTTGGCGCTGATTTTTTGCCGATTGGTCAGCACTGTATTTTc < 1:1221838/69‑1 (MQ=255) gcggATGGTGGACCATTGCAGTACACCGTTGGCGCTGATTTTTTGCCGATTGGTCAGCACTGTATTTTc < 1:1890989/69‑1 (MQ=255) gcggATGGTGGACCATTGCAGTACACCGTTGGCGCTGATTTTTTGCCGATTGGTCAGCACTGTATTTTc < 1:1802857/69‑1 (MQ=255) ggATGGTGGACCATTGCAGTACACCGTTGGCGCTGATTTTTTGCCGATTGGTCAGCACTGTATTTTc > 1:2491560/1‑67 (MQ=255) ggATGGTGGACCATTGCAGTACACCGTTGGCGCTGATTTTTTGCCGATTGGTCAGCACTGTATTTTCg > 1:2817895/1‑68 (MQ=255) ggATGGTGGACCATTGCAGTACACCGTTGGCGCTGATTTTTTGCCGATTGGTCAGCACTGTATTTTCg > 1:3237295/1‑68 (MQ=255) ggATGGTGGACAATTGCAGTACACCGTTGGCGCTGATTTTTTGCCGATTGGTCAGCACTTTATTTTCg > 1:2626921/1‑68 (MQ=255) tggtggACCATTGCAGCACACCGTTGGCGCTGATTTTTTGCCGATTGGTCAGCACTGTATTTTCGTCaa < 1:1137529/69‑1 (MQ=255) tggtggACCATTGCAGCACACCGTTGGCGCTGATTTTTTGCCGATTGGTCAGCACTGTATTTTCGTCaa < 1:3075553/69‑1 (MQ=255) tggtggACCATTGCAGCACACCGTTGGCGCTGATTTTTTGCCGATTGGTCAGCACTGTATTTTCGTCaa < 1:222351/69‑1 (MQ=255) ggACCATTGCAGCACACCGTTGGCGCTGATTTTTTGCCGATTGGTCAGCACTGTATTTTCGTCAACGc < 1:542815/68‑1 (MQ=255) ggACCATTGCAGCACACCGTTGGCGCTGATTTTTTGCCGATTGGTCAGCACTGTATTTTCGTCAACGc < 1:2343315/68‑1 (MQ=255) ggACCATTGCAGCACACCGTTGGCGCTGATTTTTTGCCGATTGGTCAGCACTGTATTTTCGTCAACGc < 1:2643188/68‑1 (MQ=255) gACCATTGCAGCACACCGTTGGCGCTGATTTTTTGCCGATTGGTCAGCACTGTATTTTCGTCAACGc < 1:1777174/67‑1 (MQ=255) tGCAGCACACCGTTGGCGCTGATTTTTTGCCGATTGGTCAGCACTGTATTTTCGTCAACGCCCAACCa > 1:611949/1‑68 (MQ=255) tGCAGCACACCGTTGGCGCTGATTTTTTGCCGATTGGTCAGCACTGTATTTTCGTCAACGCCCAACCa > 1:1430354/1‑68 (MQ=255) tGCAGCACACCGTTGGCGCTGATTTTTTGCCGATTGGTCAGCACTGTATTTTCGTCAACGCCCAACCa > 1:551409/1‑68 (MQ=255) tGCAGCACACCGTTGGCGCTGATTTTTTGCCGATTGGTCAGCACTGTATTTTCGTCAACGCCCAACCa > 1:1961006/1‑68 (MQ=255) tGCAGCACACCGTTGGCGCTGATTTTTTGCCGATTGGTCAGCACTGTATTTTCGTCAACGCCCAACCa > 1:1180070/1‑68 (MQ=255) tGCAGCACACCGTTGGCGCTGATTTTTTGCCGATTGGTCAGCACTGTATTTTCGTCAACGCCCAACCa > 1:2035143/1‑68 (MQ=255) tGCAGCACACCGTTGGCGCTGATTTTTTGCCGATTGGTCAGCACTGTATTTTCGTCAACGCCCAACCa > 1:2915095/1‑68 (MQ=255) tGCAGCACACCGTTGGCGCTGATTTTTTGCCGATTGGTCAGCACTGTATTTTCGTCAACGCCCAACCa > 1:970839/1‑68 (MQ=255) gcagcaCACCGTTGGCGCTGATTTTTTGCCGATTGGTCAGCACTGTATTTTCGTCAACGCCCAACCAg > 1:875074/1‑68 (MQ=255) | GGCGGCCTTGACCGTCATCCGCTGCATCACCTCTTCGGTCAGGCGGCGGATGGTGGACCATTGCAGCACACCGTTGGCGCTGATTTTTTGCCGATTGGTCAGCACTGTATTTTCGTCAACGCCCAACCAG > minE/1709860‑1709989 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |