| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,712,951 | T→A | 37.7% | G827G (GGA→GGT) | yphG ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,712,951 | 0 | T | A | 37.7% | 42.2 / 51.8 | 61 | G827G (GGA→GGT) | yphG | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (9/29); new base A (7/16); total (16/45) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.65e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 | |||||||||||
GTCGTCGCGTTTCAGCGCAATTTCCAGATTATTTTCCAGACGCGCCAGTCGTTTCTCCGGTGTGGCTCCACTTAACTTATCCAGCAAATCCCGTTCGAAAAGCAGACGTGCATCCTGCGGCGCAAGCTGATAAG > minE/1712885‑1713018 | gtcgtcGCGTTTCAGCGCAATTTCCAGATTATTTTCCAGACGCGCCAGTCGTTTCTCCGGTGTGGCTcc > 1:669367/1‑69 (MQ=255)gtcgtcGCGTTTCAGCGCAATTTCCAGATTATTTTCCAGACGCGCCAGTCGTTTCTCCGGTGTGGCTcc > 1:320036/1‑69 (MQ=255)gtcgtcGCGTTTCAGCGCAATTTCCAGATTATTTTCCAGACGCGCCAGTCGTTTCTCCGGTGTGGCTcc > 1:2299692/1‑69 (MQ=255)gtcgtcGCGTTTCAGCGCAATTTCCAGATTATTTTCCAGACGCGCCAGTCGTTTCTCCGGTGTGGCTc < 1:1708336/68‑1 (MQ=255) tcgtcgCGTTTCAGCGCAATTTCCAGATTATTTTCCAGACGCGCCAGTCGTTTCTCCGGTGTGGCTCCa > 1:2149060/1‑69 (MQ=255) tcgtcgCGTTTCAGCGCAATTTCCAGATTATTTTCCAGACGCGCCAGTCGTTTCTCCGGTGTGGCTCCa > 1:1471480/1‑69 (MQ=255) tcgtcgCGTTTCAGCGCAATTTCCAGATTATTTTCCAGACGCGCCAGTCGTTTCTCCGGTGTGGCTCCa > 1:146579/1‑69 (MQ=255) cgcgTTTCAGCGCAATTTCCAGATTATTTTCCAGACGCGCCAGTCGTTTCTCCGGTGTGGCTCCactta < 1:1115313/69‑1 (MQ=255) cgcgTTTCAGCGCAATTTCCAGATTATTTTCCAGACGCGCCAGTCGTTTCTCCGGTGTGGCTCCactta < 1:1969889/69‑1 (MQ=255) cgcgTTTCAGCGCAATTTCCAGATTATTTTCCAGACGCGCCAGTCGTTTCTCCGGTGTGGCTCCactta < 1:1448495/69‑1 (MQ=255) aTTTCCAGATTATTTTCCAGACGCGCCAGTCGTTTCTCCGGTGTGCCACCGCTTAACTTATCCAGCaaa > 1:1892127/1‑69 (MQ=255) aTTTCCAGATTATTTTCCAGACGCGCCAGTCGTTTCTCCGGTGTGACACCGCTTAAGTTATCCAGCaaa > 1:2523390/1‑69 (MQ=255) aTTTCCAGATTATTTTCCAGACGCGCCAGTCGTTTCTCCGGTGTGACACCGCTTAACTTATCCAGCaaa > 1:793490/1‑69 (MQ=255) aTTTCCAGATTATTTTCCAGACGCGCCAGTCGTTTCTCCGGTGTGACACCGCTTAACTTATCCAGCaaa > 1:2703612/1‑69 (MQ=255) aTTTCCAGATTATTTTCCAGACGCGCCAGTCGTTTCTCCGGTGTGACACCGCTTAACTTATCCAGCaaa > 1:1718069/1‑69 (MQ=255) aTTTCCAGATTATTTTCCAGACGCGCCAGTCGTTTCTCCGGTGTGACACCGCTTAACTTATCCAGCaaa > 1:2774016/1‑69 (MQ=255) aTTTCCAGATTATTTTCCAGACGCGCCAGTCGTTTCTCCGGTGTGACACCGCTTAACTTATCCAGCaaa > 1:2847465/1‑69 (MQ=255) aGATTATTTTCCAGACGCGCCAGTCGTTTCTCCGGTGTGGCTCCACTTAACTTATCCAGCAAATCCCg < 1:3192503/68‑1 (MQ=255) aGATTATTTTCCAGACGCGCCAGTCGTTTCTCCGGTGTGGCTCCACTTAACTTATCCAGCAAATCCCg < 1:963194/68‑1 (MQ=255) aGATTATTTTCCAGACGCGCCAGTCGTTTCTCCGGTGTGGCTCCACTTAACTTATCCAGCAAATCCCg < 1:2553207/68‑1 (MQ=255) aGATTATTTTCCAGACGCGCCAGTCGTTTCTCCGGTGTGGCTCCACTTAACTTATCCAGCAAATCCCg < 1:2295028/68‑1 (MQ=255) aGATTATTTTCCAGACGCGCCAGTCGTTTCTCCGGTGTGGCTCCACTTAACTTATCCAGCAAATCCCg < 1:803095/68‑1 (MQ=255) aGATTATTTTCCAGACGCGCCAGTCGTTTCTCCGGTGTGGCTCCACTTAACTTATCCAGCAAATCCCg < 1:115100/68‑1 (MQ=255) aGATTATTTTCCAGACGCGCCAGTCGTTTCTCCGGTGTGGCTCCACTTAACTTATCCAGCAAATCCCg < 1:174007/68‑1 (MQ=255) gATTATTTTCCAGACGCGCCAGTCGTTTCTCCGGTGTGGCTCCACTTAACTTATCCAGCAAATCCCg < 1:1665831/67‑1 (MQ=255) ttattTTCCAGACGCGCCAGTCGTTTCTCCGGTGTGGCTCCACTTAACTTATCCAGCAAATCCCGtt > 1:2901967/1‑67 (MQ=255) ttattTTCCAGACGCGCCAGTCGTTTCTCCGGTGTGGCTCCACTTAACTTATCCAGCAAATCCCGtt > 1:518270/1‑67 (MQ=255) ttattTTCCAGACGCGCCAGTCGTTTCTCCGGTGTGGCTCCACTTAACTTATCCAGCAAATCCCGTTc > 1:3215107/1‑68 (MQ=255) cAGACGCGCCAGTCGTTTCTCCGGTGTGGCTCCACTTAACTTATCCAGCAAATCCCGTTCGAAAAGCa < 1:946221/68‑1 (MQ=255) cGTTTCTCCGGTGTGGCTCCACTTAACTTATCCAGCAAATCCCGTTCGAAAAGCAGACGTGCa < 1:1402191/63‑1 (MQ=255) cGTTTCTCCGGTGTGGCTCCACTTAACTTATCCAGCAAATCCCGTTCGAAAAGCAGACGTGCa < 1:115434/63‑1 (MQ=255) tcCGGTGTGACACCGCTTAACTTATCCAGCAAATCCCGTTCGa < 1:3137012/43‑1 (MQ=37) tcCGGTGTGACACCGCTTAACTTATCCAGCAAATCCCGTTCGa < 1:3025847/43‑1 (MQ=37) tcCGGTGTGACACCGCTTAACTTATCCAGCAAATCCCGTTCGa < 1:46697/43‑1 (MQ=37) tcCGGTGTGACACCGCTTAACTTATCCAGCAAATCCCGTTCGa < 1:65507/43‑1 (MQ=37) tcCGGTGTGACACCGCTTAACTTATCCAGCAAATCCCGTTCGa < 1:2605768/43‑1 (MQ=37) tcCGGTGTGACACCGCTTAACTTATCCAGCAAATCCCGTTCGa < 1:10219/43‑1 (MQ=37) tcCGGTGTGACACCGCTTAACTTATCCAGCAAATCCCGTTCGa < 1:2504299/43‑1 (MQ=37) tcCGGTGTGACACCGCTTAACTTATCCAGCAAATCCCGTTCGa < 1:144351/43‑1 (MQ=37) tcCGGTGTGACACCGCTTAACTTATCCAGCAAATCCCGTTCGa < 1:1504680/43‑1 (MQ=37) tcCGGTGTGACACCGCTTAACTTATCCAGCAAATCCCGTTCGa < 1:1566458/43‑1 (MQ=37) tcCGGTGTGACACCGCTTAACTTATCCAGCAAATCCCGTTCGa < 1:1403923/43‑1 (MQ=37) tcCGGTGTGACACCGCTTAACTTATCCAGCAAATCCCGTTCGa < 1:1747697/43‑1 (MQ=37) tcCGGTGTGACACCGCTTAACTTATCCAGCAAATCCCGTTCGa < 1:1773934/43‑1 (MQ=37) tcCGGTGTGACACCGCTTAACTTATCCAGCAAATCCCGTTCGa < 1:2461721/43‑1 (MQ=37) tcCGGGGTGACACCGCTTAACTTATCCAGCAAATCCCGTTCGa < 1:2254755/43‑1 (MQ=25) ccGGTGTGACACCGCTTAACTTATCCAGCAAATCCCGTTCGa < 1:2943809/42‑1 (MQ=37) gtgtGGCTCCACTTAACTTATCCAGCAAATCCCGTTCGAAAAGCAGACGTGCATCCTGCGGCGCAAGCt < 1:1990357/69‑1 (MQ=255) gtgtGGCTCCACTTAACTTATCCAGCAAATCCCGTTCGAAAAGCAGACGTGCATCCTGCGGCGCAAGCt < 1:900110/69‑1 (MQ=255) gtgtGGCTCCACTTAACTTATCCAGCAAATCCCGTTCGAAAAGCAGACGTGCATCCTGCGGCGCAAGCt < 1:1650561/69‑1 (MQ=255) gtgtGGCTCCACTTAACTTATCCAGCAAATCCCGTTCGAAAAGCAGACGTGCATCCTGCGGCGCAAGCt < 1:1719918/69‑1 (MQ=255) gtgtGGCTCCACTTAACTTATCCAGCAAATCCCGTTCGAAAAGCAGACGTGCATCCTGCGGCGCAAGCt < 1:1994317/69‑1 (MQ=255) gtgtGGCTCCACTTAACTTATCCAGCAAATCCCGTTCGAAAAGCAGACGTGCATCCTGCGGCGCAAGCt < 1:1362976/69‑1 (MQ=255) gtgtGGCTCCACTTAACTTATCCAGCAAATCCCGTTCGAAAAGCAGACGTGCATCCTGCGGCGCAAGCt < 1:2914256/69‑1 (MQ=255) gtgtGGCTCCACTTAACTTATCCAGCAAATCCCGTTCGAAAAGCAGACGTGCATCCTGCGGCGCAAGCt < 1:2197512/69‑1 (MQ=255) gtgtGGCTCCACTTAACTTATCCAGCAAATCCCGTTCGAAAAGCAGACGTGCATCCTGCGGCGCAAGCt < 1:2321122/69‑1 (MQ=255) cTCCACTTAACTTATCCAGCAAATCCCGTTCGAAAAGCAGACGTGCATCCTGCGGCGCAAGCTg < 1:31099/64‑1 (MQ=255) cTCCACTTAACTTATCCAGCAAATCCCGTTCGAAAAGCAGACGTGCATCCTGCGGCGCAAGCTg < 1:3028723/64‑1 (MQ=255) cTCCACTTAACTTATCCAGCAAATCCCGTTCGAAAAGCAGACGTGCATCCTGCGGCGCAAGCTg < 1:2316908/64‑1 (MQ=255) cTCCACTTAACTTATCCAGCAAATCCCGTTCGAAAAGCAGACGTGCATCCTGCGGCGCAAGCTg < 1:960488/64‑1 (MQ=255) cTCCACTTAACTTATCCAGCAAATCCCGTTCGAAAAGCAGACGTGCATCCTGCGGCGCAAGCTg < 1:2459763/64‑1 (MQ=255) tCCACTTAACTTATCCAGCAAATCCCGTTCGAAAAGCAGACGTGCATCCTGCGGCGCAAGCTGATAAg < 1:1876674/68‑1 (MQ=255) tCCACTTAACTTATCCAGCAAATCCCGTTCGAAAAGCAGACGTGCATCCTGCGGCGCAAGCTGATAAg < 1:1581959/68‑1 (MQ=255) | GTCGTCGCGTTTCAGCGCAATTTCCAGATTATTTTCCAGACGCGCCAGTCGTTTCTCCGGTGTGGCTCCACTTAACTTATCCAGCAAATCCCGTTCGAAAAGCAGACGTGCATCCTGCGGCGCAAGCTGATAAG > minE/1712885‑1713018 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |