Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,712,954 A→G 42.6% S826S (AGT→AGC yphG ← conserved hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,712,9540AG42.6% 12.5 / 39.6 54S826S (AGT→AGCyphGconserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (3/28);  new base G (7/16);  total (10/44)
Fisher's exact test for biased strand distribution p-value = 7.79e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.24e-01

TCGTCGCGTTTCAGCGCAATTTCCAGATTATTTTCCAGACGCGCCAGTCGTTTCTCCGGTGTGGCTCCACTTAACTTATCCAGCAAATCCCGTTCGAAAAGCAGACGTGCATCCTGCGGCGCAAGCTGATAAG  >  minE/1712886‑1713018
                                                                    |                                                                
tcgtcgCGTTTCAGCGCAATTTCCAGATTATTTTCCAGACGCGCCAGTCGTTTCTCCGGTGTGGCTCCa                                                                  >  1:2149060/1‑69 (MQ=255)
tcgtcgCGTTTCAGCGCAATTTCCAGATTATTTTCCAGACGCGCCAGTCGTTTCTCCGGTGTGGCTCCa                                                                  >  1:1471480/1‑69 (MQ=255)
tcgtcgCGTTTCAGCGCAATTTCCAGATTATTTTCCAGACGCGCCAGTCGTTTCTCCGGTGTGGCTCCa                                                                  >  1:146579/1‑69 (MQ=255)
    cgcgTTTCAGCGCAATTTCCAGATTATTTTCCAGACGCGCCAGTCGTTTCTCCGGTGTGGCTCCactta                                                              <  1:1115313/69‑1 (MQ=255)
    cgcgTTTCAGCGCAATTTCCAGATTATTTTCCAGACGCGCCAGTCGTTTCTCCGGTGTGGCTCCactta                                                              <  1:1969889/69‑1 (MQ=255)
    cgcgTTTCAGCGCAATTTCCAGATTATTTTCCAGACGCGCCAGTCGTTTCTCCGGTGTGGCTCCactta                                                              <  1:1448495/69‑1 (MQ=255)
                  aTTTCCAGATTATTTTCCAGACGCGCCAGTCGTTTCTCCGGTGTGCCACCGCTTAACTTATCCAGCaaa                                                >  1:1892127/1‑69 (MQ=255)
                  aTTTCCAGATTATTTTCCAGACGCGCCAGTCGTTTCTCCGGTGTGACACCGCTTAAGTTATCCAGCaaa                                                >  1:2523390/1‑69 (MQ=255)
                  aTTTCCAGATTATTTTCCAGACGCGCCAGTCGTTTCTCCGGTGTGACACCGCTTAACTTATCCAGCaaa                                                >  1:2774016/1‑69 (MQ=255)
                  aTTTCCAGATTATTTTCCAGACGCGCCAGTCGTTTCTCCGGTGTGACACCGCTTAACTTATCCAGCaaa                                                >  1:2703612/1‑69 (MQ=255)
                  aTTTCCAGATTATTTTCCAGACGCGCCAGTCGTTTCTCCGGTGTGACACCGCTTAACTTATCCAGCaaa                                                >  1:2847465/1‑69 (MQ=255)
                  aTTTCCAGATTATTTTCCAGACGCGCCAGTCGTTTCTCCGGTGTGACACCGCTTAACTTATCCAGCaaa                                                >  1:793490/1‑69 (MQ=255)
                  aTTTCCAGATTATTTTCCAGACGCGCCAGTCGTTTCTCCGGTGTGACACCGCTTAACTTATCCAGCaaa                                                >  1:1718069/1‑69 (MQ=255)
                        aGATTATTTTCCAGACGCGCCAGTCGTTTCTCCGGTGTGGCTCCACTTAACTTATCCAGCAAATCCCg                                           <  1:3192503/68‑1 (MQ=255)
                        aGATTATTTTCCAGACGCGCCAGTCGTTTCTCCGGTGTGGCTCCACTTAACTTATCCAGCAAATCCCg                                           <  1:2553207/68‑1 (MQ=255)
                        aGATTATTTTCCAGACGCGCCAGTCGTTTCTCCGGTGTGGCTCCACTTAACTTATCCAGCAAATCCCg                                           <  1:2295028/68‑1 (MQ=255)
                        aGATTATTTTCCAGACGCGCCAGTCGTTTCTCCGGTGTGGCTCCACTTAACTTATCCAGCAAATCCCg                                           <  1:803095/68‑1 (MQ=255)
                        aGATTATTTTCCAGACGCGCCAGTCGTTTCTCCGGTGTGGCTCCACTTAACTTATCCAGCAAATCCCg                                           <  1:963194/68‑1 (MQ=255)
                        aGATTATTTTCCAGACGCGCCAGTCGTTTCTCCGGTGTGGCTCCACTTAACTTATCCAGCAAATCCCg                                           <  1:174007/68‑1 (MQ=255)
                        aGATTATTTTCCAGACGCGCCAGTCGTTTCTCCGGTGTGGCTCCACTTAACTTATCCAGCAAATCCCg                                           <  1:115100/68‑1 (MQ=255)
                         gATTATTTTCCAGACGCGCCAGTCGTTTCTCCGGTGTGGCTCCACTTAACTTATCCAGCAAATCCCg                                           <  1:1665831/67‑1 (MQ=255)
                           ttattTTCCAGACGCGCCAGTCGTTTCTCCGGTGTGGCTCCACTTAACTTATCCAGCAAATCCCGtt                                         >  1:518270/1‑67 (MQ=255)
                           ttattTTCCAGACGCGCCAGTCGTTTCTCCGGTGTGGCTCCACTTAACTTATCCAGCAAATCCCGtt                                         >  1:2901967/1‑67 (MQ=255)
                           ttattTTCCAGACGCGCCAGTCGTTTCTCCGGTGTGGCTCCACTTAACTTATCCAGCAAATCCCGTTc                                        >  1:3215107/1‑68 (MQ=255)
                                   cAGACGCGCCAGTCGTTTCTCCGGTGTGGCTCCACTTAACTTATCCAGCAAATCCCGTTCGAAAAGCa                                <  1:946221/68‑1 (MQ=255)
                                                cGTTTCTCCGGTGTGGCTCCACTTAACTTATCCAGCAAATCCCGTTCGAAAAGCAGACGTGCa                        <  1:115434/63‑1 (MQ=255)
                                                cGTTTCTCCGGTGTGGCTCCACTTAACTTATCCAGCAAATCCCGTTCGAAAAGCAGACGTGCa                        <  1:1402191/63‑1 (MQ=255)
                                                      tcCGGTGTGACACCGCTTAACTTATCCAGCAAATCCCGTTCGa                                      <  1:46697/43‑1 (MQ=37)
                                                      tcCGGTGTGACACCGCTTAACTTATCCAGCAAATCCCGTTCGa                                      <  1:1504680/43‑1 (MQ=37)
                                                      tcCGGTGTGACACCGCTTAACTTATCCAGCAAATCCCGTTCGa                                      <  1:2605768/43‑1 (MQ=37)
                                                      tcCGGTGTGACACCGCTTAACTTATCCAGCAAATCCCGTTCGa                                      <  1:1403923/43‑1 (MQ=37)
                                                      tcCGGTGTGACACCGCTTAACTTATCCAGCAAATCCCGTTCGa                                      <  1:3025847/43‑1 (MQ=37)
                                                      tcCGGTGTGACACCGCTTAACTTATCCAGCAAATCCCGTTCGa                                      <  1:3137012/43‑1 (MQ=37)
                                                      tcCGGTGTGACACCGCTTAACTTATCCAGCAAATCCCGTTCGa                                      <  1:144351/43‑1 (MQ=37)
                                                      tcCGGTGTGACACCGCTTAACTTATCCAGCAAATCCCGTTCGa                                      <  1:65507/43‑1 (MQ=37)
                                                      tcCGGTGTGACACCGCTTAACTTATCCAGCAAATCCCGTTCGa                                      <  1:10219/43‑1 (MQ=37)
                                                      tcCGGTGTGACACCGCTTAACTTATCCAGCAAATCCCGTTCGa                                      <  1:2504299/43‑1 (MQ=37)
                                                      tcCGGTGTGACACCGCTTAACTTATCCAGCAAATCCCGTTCGa                                      <  1:1747697/43‑1 (MQ=37)
                                                      tcCGGTGTGACACCGCTTAACTTATCCAGCAAATCCCGTTCGa                                      <  1:1773934/43‑1 (MQ=37)
                                                      tcCGGTGTGACACCGCTTAACTTATCCAGCAAATCCCGTTCGa                                      <  1:1566458/43‑1 (MQ=37)
                                                      tcCGGTGTGACACCGCTTAACTTATCCAGCAAATCCCGTTCGa                                      <  1:2461721/43‑1 (MQ=37)
                                                      tcCGGGGTGACACCGCTTAACTTATCCAGCAAATCCCGTTCGa                                      <  1:2254755/43‑1 (MQ=25)
                                                       ccGGTGTGACACCGCTTAACTTATCCAGCAAATCCCGTTCGa                                      <  1:2943809/42‑1 (MQ=37)
                                                          gtgtGGCTCCACTTAACTTATCCAGCAAATCCCGTTCGAAAAGCAGACGTGCATCCTGCGGCGCAAGCt        <  1:1362976/69‑1 (MQ=255)
                                                          gtgtGGCTCCACTTAACTTATCCAGCAAATCCCGTTCGAAAAGCAGACGTGCATCCTGCGGCGCAAGCt        <  1:900110/69‑1 (MQ=255)
                                                          gtgtGGCTCCACTTAACTTATCCAGCAAATCCCGTTCGAAAAGCAGACGTGCATCCTGCGGCGCAAGCt        <  1:1719918/69‑1 (MQ=255)
                                                          gtgtGGCTCCACTTAACTTATCCAGCAAATCCCGTTCGAAAAGCAGACGTGCATCCTGCGGCGCAAGCt        <  1:1990357/69‑1 (MQ=255)
                                                          gtgtGGCTCCACTTAACTTATCCAGCAAATCCCGTTCGAAAAGCAGACGTGCATCCTGCGGCGCAAGCt        <  1:1994317/69‑1 (MQ=255)
                                                          gtgtGGCTCCACTTAACTTATCCAGCAAATCCCGTTCGAAAAGCAGACGTGCATCCTGCGGCGCAAGCt        <  1:1650561/69‑1 (MQ=255)
                                                          gtgtGGCTCCACTTAACTTATCCAGCAAATCCCGTTCGAAAAGCAGACGTGCATCCTGCGGCGCAAGCt        <  1:2197512/69‑1 (MQ=255)
                                                          gtgtGGCTCCACTTAACTTATCCAGCAAATCCCGTTCGAAAAGCAGACGTGCATCCTGCGGCGCAAGCt        <  1:2914256/69‑1 (MQ=255)
                                                          gtgtGGCTCCACTTAACTTATCCAGCAAATCCCGTTCGAAAAGCAGACGTGCATCCTGCGGCGCAAGCt        <  1:2321122/69‑1 (MQ=255)
                                                                cTCCACTTAACTTATCCAGCAAATCCCGTTCGAAAAGCAGACGTGCATCCTGCGGCGCAAGCTg       <  1:31099/64‑1 (MQ=255)
                                                                cTCCACTTAACTTATCCAGCAAATCCCGTTCGAAAAGCAGACGTGCATCCTGCGGCGCAAGCTg       <  1:3028723/64‑1 (MQ=255)
                                                                cTCCACTTAACTTATCCAGCAAATCCCGTTCGAAAAGCAGACGTGCATCCTGCGGCGCAAGCTg       <  1:2316908/64‑1 (MQ=255)
                                                                cTCCACTTAACTTATCCAGCAAATCCCGTTCGAAAAGCAGACGTGCATCCTGCGGCGCAAGCTg       <  1:960488/64‑1 (MQ=255)
                                                                cTCCACTTAACTTATCCAGCAAATCCCGTTCGAAAAGCAGACGTGCATCCTGCGGCGCAAGCTg       <  1:2459763/64‑1 (MQ=255)
                                                                 tCCACTTAACTTATCCAGCAAATCCCGTTCGAAAAGCAGACGTGCATCCTGCGGCGCAAGCTGATAAg  <  1:1581959/68‑1 (MQ=255)
                                                                 tCCACTTAACTTATCCAGCAAATCCCGTTCGAAAAGCAGACGTGCATCCTGCGGCGCAAGCTGATAAg  <  1:1876674/68‑1 (MQ=255)
                                                                  ccACTTAACTTATCCAGCAAATCCCGTTCGAAAAGCAGACGTGCATCCTGCGGCGCAAGCTGATAAg  <  1:1738186/67‑1 (MQ=255)
                                                                    |                                                                
TCGTCGCGTTTCAGCGCAATTTCCAGATTATTTTCCAGACGCGCCAGTCGTTTCTCCGGTGTGGCTCCACTTAACTTATCCAGCAAATCCCGTTCGAAAAGCAGACGTGCATCCTGCGGCGCAAGCTGATAAG  >  minE/1712886‑1713018

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: