Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | minE | 1,714,265 | A→G | 53.0% | G389G (GGT→GGC) | yphG ← | conserved hypothetical protein |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | minE | 1,714,265 | 0 | A | G | 53.0% | 8.5 / 70.1 | 83 | G389G (GGT→GGC) | yphG | conserved hypothetical protein |
Reads supporting (aligned to +/- strand): ref base A (17/22); new base G (38/6); total (55/28) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 5.53e-05 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.11e-01 |
GATGGCGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGCCCCCACTC > minE/1714211‑1714327 | gATGGCGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGAt > 1:3083748/1‑69 (MQ=255) gATGGCGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGAt > 1:2635151/1‑69 (MQ=255) gATGGCGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGAt > 1:2688924/1‑69 (MQ=255) gATGGCGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGAt > 1:698738/1‑69 (MQ=255) gATGGCGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGAt > 1:1761525/1‑69 (MQ=255) gATGGCGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGAt > 1:1388155/1‑69 (MQ=255) tGGCGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGATc > 1:2094588/1‑68 (MQ=255) gTCGCTGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTGCCg > 1:1813407/1‑51 (MQ=255) gTCGCTGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTGCCGATTTCGCGGATCGCCAgg > 1:2608216/1‑69 (MQ=255) gTCGCTGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTGCCGATTTCGCGGATCGCCAgg > 1:282259/1‑69 (MQ=255) gTCGCTGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTGCCGATTTCGCGGATCGCCAgg > 1:2631951/1‑69 (MQ=255) gTCGCTGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTGCCGATTTCGCGGATCGCCAgg > 1:167189/1‑69 (MQ=255) gTCGCTGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTGCCGATTTCGCGGATCGCCAgg > 1:2950377/1‑69 (MQ=255) gTCGCTGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTGCCGATTTCGCGGATCGCCAgg > 1:925380/1‑69 (MQ=255) gTCGCTGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTGCCGATTTCGCGGATCGCCAgg > 1:194584/1‑69 (MQ=255) gTCGCTGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTGCCGATTTCGCGGATCGCCAgg > 1:3167418/1‑69 (MQ=255) gTCGCTGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTGCCGATTTCGCGGATCGCCAgg > 1:2748582/1‑69 (MQ=255) gTCGCTGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTGCCGATTTCGCGGATCGCCAgg > 1:325336/1‑69 (MQ=255) gTCGCTGGCATCAGTGCCAAGGCATCATCAAGTAACGCGTAGCATTTGCCGATTTCGCGGATCGCCAgg > 1:291503/1‑69 (MQ=255) aTCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCg > 1:871734/1‑69 (MQ=255) aGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTc > 1:2495027/1‑69 (MQ=255) aGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTc > 1:2330607/1‑69 (MQ=255) aGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTc > 1:2195038/1‑69 (MQ=255) aGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTc > 1:75102/1‑69 (MQ=255) aCGGCATGATCAAGTAACGCGTTGCATTTGCCGATTTCGCGGATCGCCAGGCGGTATCCGTTCa > 1:144312/1‑64 (MQ=255) aCGGCATCATCAAGTAACGCGTTGCATTTGCCGATTTc > 1:2710799/1‑38 (MQ=255) aCGGCATCATCAAGTAACGCGTTGCATTTGCCGATTTCGCgg > 1:2173363/1‑42 (MQ=255) aCGGCATCATCAAGTAACGCGTTGCATTTGCCGATTTCGCGGATCGCCAGGCGGt > 1:1111006/1‑55 (MQ=255) aCGGCATCATCAAGTAACGCGTTGCATTTGCCGATTTCGCGGATCGCCAGGCGGTATCCGTTCa > 1:2324686/1‑64 (MQ=255) aCGGCATCATCAAGTAACGCGTTGCATTTGCCGATTTCGCGGATCGCCAGGCGGTATCCGTTCa > 1:2753187/1‑64 (MQ=255) aCGGCATCATCAAGTAACGCGTTGCATTTGCCGATTTCGCGGATCGCCAGGCGGTATCCGTTCa > 1:3429649/1‑64 (MQ=255) aCGGCATCATCAAGTAACGCGTTGCATTTGCCGATTTCGCGGATCGCCAGGCGGTATCCGTTCa > 1:2777530/1‑64 (MQ=255) aCGGCATCATCAAGTAACGCGTTGCATTTGCCGATTTCGCGGATCGCCAGGCGGTATCCGTTCa > 1:311187/1‑64 (MQ=255) aCGGCATCATCAAGTAACGCGTTGCATTTGCCGATTTCGCGGATCGCCAGGCGGTATCCGTTCa > 1:2861030/1‑64 (MQ=255) aCGGCATCATCAAGTAACGCGTTGCATTTGCCGATTTCGCGGATCGCCAGGCGGTATCCGTTCa > 1:2542195/1‑64 (MQ=255) aCGGCATCATCAAGTAACGCGTTGCATTTGCCGATTTCGCGGATCGCCAGGCGGTATCCGTTCa > 1:2534468/1‑64 (MQ=255) aCGGCATCATCAAGTAACGCGTTGCATTTGCCGATTTCGCGGATCGCCAGGCGGTATCCGTTCa > 1:2886389/1‑64 (MQ=255) aCGGCATCATCAAGTAACGCGTTGCATTTGCCGATTTCGCGGATCGCCAGGCGGTATCCGTTCa > 1:555129/1‑64 (MQ=255) aCGGCATCATCAAGTAACGCGTTGCATTTGCCGATTTCGCGGATCGCCAGGCGGTATCCGTTCa > 1:1121546/1‑64 (MQ=255) aCGGCATCATCAAGTAACGCGTTGCATTTGCCGATTTCGCGGATCGCCAGGCGGTATCCGTTCa > 1:1123664/1‑64 (MQ=255) aCGGCATCATCAAGTAACGCGTTGCATTTGCCGATTTCGCGGATCGCCAGGCGGTATCCGTTCa > 1:1209598/1‑64 (MQ=255) aCGGCATCATCAAGTAACGCGTTGCATTTGCCGATTTCGCGGATCGCCAGGCGGTATCCGTTCa > 1:769877/1‑64 (MQ=255) aCGGCATCATCAAGTAACGCGTTGCATTTGCCGATTTCGCGGATCGCCAGGCGGTATCCGTTCa > 1:2171583/1‑64 (MQ=255) aCGGCATCATCAAGTAACGCGTTGCATTTGCCGATTTCGCGGATCGCCAGGCGGTATCCGTTCa > 1:209427/1‑64 (MQ=255) aCGGCATCATCAAGTAACGCGTTGCATTTGCCGATTTCGCGGATCGCCAGGCGGTATCCGTTCa > 1:1432519/1‑64 (MQ=255) aCGGCATCATCAAGTAACGCGTTGCATTTGCCGATTTCGCGGATCGCCAGGCGGTATCCGTTCa > 1:1543212/1‑64 (MQ=255) aCGGCATCATCAAGTAACGCGTTGCATTTGCCGATTTCGCGGATCGCCAGGCGGTATCCGTTCa > 1:1789886/1‑64 (MQ=255) aCGGCATCATCAAGTAACGCGTTGCATTTGACGATTTCGCGGATc > 1:1325133/1‑45 (MQ=255) aCGGCATCATCAAGTAACGCGCTGCATTTGCCGATTTCGCGGATCGCCAGGCGGTATCCGTTCa > 1:2138640/1‑64 (MQ=255) aCGGCATCATCAAGTAAAGCGTTGCATTTGCCGATTTCGCGGATCGCCAGGCGGTATCCGTTCa > 1:2342894/1‑64 (MQ=255) gCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGgaga < 1:1076197/68‑1 (MQ=255) gCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGgaga < 1:2988342/68‑1 (MQ=255) gCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGgaga < 1:1992002/68‑1 (MQ=255) gCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGgaga < 1:2070819/68‑1 (MQ=255) gCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGgaga < 1:2347792/68‑1 (MQ=255) gCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGgaga < 1:3434902/68‑1 (MQ=255) gCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGgaga < 1:395512/68‑1 (MQ=255) catcatCAAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGgagaga < 1:362030/69‑1 (MQ=255) tcatcaAGTAACGCGTTGCATTTGCCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATg < 1:556697/69‑1 (MQ=255) tcatcaAGTAACGCGTTGCATTTGCCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATg < 1:2600821/69‑1 (MQ=255) tcatcaAGTAACGCGTTGCATTTGCCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATg < 1:487635/69‑1 (MQ=255) tcatcaAGTAACGCGTTGCATTTGCCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATg < 1:2367651/69‑1 (MQ=255) tcatcaAGTAACGCGTTGCATTTGCCGATTTCGCGGATCGCCAGGCGGTATCCGGTCAACGGAGAGATg < 1:778048/69‑1 (MQ=255) tcatcaAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATg < 1:977492/69‑1 (MQ=255) tcatcaAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATg < 1:212674/69‑1 (MQ=255) tcatcaAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATg < 1:637760/69‑1 (MQ=255) tcatcaAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATg < 1:2309152/69‑1 (MQ=255) tcatcaAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATg < 1:2542941/69‑1 (MQ=255) tcatcaAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATg < 1:2723261/69‑1 (MQ=255) tcatcaAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATg < 1:3310334/69‑1 (MQ=255) tcatcaAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATg < 1:2744849/69‑1 (MQ=255) catcaAGTAACGCGTTGCATTTGCCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGAtt < 1:1477923/68‑2 (MQ=255) caAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGtt > 1:805895/1‑52 (MQ=255) caAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGct < 1:2581680/68‑2 (MQ=255) caAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCa > 1:2751586/1‑68 (MQ=255) caAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCa > 1:1161102/1‑68 (MQ=255) aaGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATa < 1:1055164/69‑1 (MQ=255) aaGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATa < 1:455284/69‑1 (MQ=255) aaGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATa < 1:2422122/69‑1 (MQ=255) aaGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATa < 1:1317778/69‑1 (MQ=255) aaGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATa < 1:3032398/69‑1 (MQ=255) gCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGCCCCCACtg > 1:3040398/1‑68 (MQ=255) gCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGCCCCCACTc > 1:1195094/1‑69 (MQ=255) | GATGGCGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGCCCCCACTC > minE/1714211‑1714327 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |