| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 130,906 | T→A | 22.2% | D362E (GAT→GAA) | yadE → | predicted polysaccharide deacetylase lipoprotein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 130,906 | 0 | T | A | 22.2% | 121.4 / 29.9 | 72 | D362E (GAT→GAA) | yadE | predicted polysaccharide deacetylase lipoprotein |
| Reads supporting (aligned to +/- strand): ref base T (25/31); new base A (9/7); total (34/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.71e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATCTTTCGTATCCGTTTGGCGGATTTAATGACAACGCCGTGAAGGCAGCAAACGATGCCGGATTTCACCTGGCGGTGACAACCATGAAAGGCAAAGTAAAACCGGGGGATAATCCGTTGT > minE/130851‑130970 | aTCTTTCTTATCCATTTGGCGGATTTAATGACAAAGCCGTGAAGTCAGCAAAAGAAGCCg > 1:829830/1‑60 (MQ=255)aTCTTTCTTATCCATTTGGCGGATTTAATGACAAAGCCGTGAAGGCAGCAAAAGAAGCCg > 1:1043852/1‑60 (MQ=255)aTCTTTCTTATCCATTTGGCGGATTTAATGACAAAGCCGTGAAGGCAGCAAAAGAAGCCg > 1:911449/1‑60 (MQ=255)aTCTTTCTTATCCATTTGGCGGATTTAATGACAAAGCCGTGAAGGCAGCAAAAGAAGCCg > 1:1175008/1‑60 (MQ=255)aTCTTTCTTATCCATTTGGCGGATTTAATGACAAAGCCGTGAAGGCAGCAAAAGAAGCCg > 1:525006/1‑60 (MQ=255)aTCTTTCTTATCCATTTGGCGGATTTAATGACAAAGCCGTGAAGGCAGCAAAAGAAGCCg > 1:2595545/1‑60 (MQ=255)aTCTTTCTTATCCATTTGGCGGATTTAATGACAAAGCCGTGAAGGCAGCAAAAGAAGCCg > 1:1986299/1‑60 (MQ=255)aTCTTTCTTATCCATTTGGCGGATTTAATGACAAAGCCGTGAAGGCAGCAAAAGAAGCCg > 1:2593862/1‑60 (MQ=255)aTCTTTCTTATCCATTTGGCGGATTTAATGACAAAGCCGTGAAGGCAGCAAAAGAAGCCg > 1:2852159/1‑60 (MQ=255)aTCTTTCGTATCCGTTTGGCGGATTTAATGACAACGCCGTGAAGGCAGCAAACGAt < 1:2384363/56‑1 (MQ=255) tCTTTCGTATCCGTTTGGCGGATTTAATGACAACGCCGTGAAGGCAGCAAACGATGCCGGATTTCACCt > 1:2314445/1‑69 (MQ=255) tttCGTATCCGTTTGGCGGATTTAATGACAACGCCGTGAAGGCAGCAAACGATGCCGGATTTCACCTgg > 1:1964159/1‑69 (MQ=255) tATCCGTTTTGCGGATTTAATGACAACGCCGTGAAGGCAGCAAACGATGCCGGATTTCACCTGGCGGt < 1:2611743/68‑1 (MQ=255) tATCCGTTTGGCGGATTTAATGACAACGCCGTGAAGGCAGCAAACGATGCCGGATTTCACCTGGCGGt < 1:1858505/68‑1 (MQ=255) tATCCGTTTGGCGGATTTAATGACAACGCCGTGAAGGCAGCAAACGATGCCGGATTTCACCTGGCGGt < 1:2936829/68‑1 (MQ=255) tATCCGTTTGGCGGATTTAATGACAACGCCGTGAAGGCAGCAAACGATGCCGGATTTCACCTGGCGGt < 1:1978126/68‑1 (MQ=255) tATCCGTTTGGCGGATTTAATGACAACGCCGTGAAGGCAGCAAACGATGCCGGATTTCACCTGGCGGt < 1:1984221/68‑1 (MQ=255) tATCCGTTTGGCGGATTTAATGACAACGCCGTGAAGGCAGCAAACGATGCCGGATTTCACCTGGCGGt < 1:2718413/68‑1 (MQ=255) tATCCGTTTGGCGGATTTAATGACAACGCCGTGAAGGCAGCAAACGATGCCGGATTTCACCTGGCGGt < 1:2088836/68‑1 (MQ=255) tATCCGTTTGGCGGATTTAATGACAACGCCGTGAAGGCAGCAAACGATGCCGGATTTCACCTGGCGGt < 1:1631644/68‑1 (MQ=255) tATCCGTTTGGCGGATTTAATGACAACGCCGTGAAGGCAGCAAACGATGCCGGATTTCACCTGGCGGt < 1:1562204/68‑1 (MQ=255) tATCCGTTTGGCGGATTTAATGACAACGCCGTGAAGGCAGCAAACGATGCCGGATTTCACCTGGCGGt < 1:2882198/68‑1 (MQ=255) tATCCGTTTGGCGGATTTAATGACAACGCCGTGAAGGCAGCAAACGATGCCGGATTTCACCTGGCGGt < 1:1468762/68‑1 (MQ=255) tATCCGTTTGGCGGATTTAATGACAACGCCGTGAAGGCAGCAAACGATGCCGGATTTCACCTGGCGGt < 1:3171169/68‑1 (MQ=255) tATCCGTTTGGCGGATTTAATGACAACGCCGTGAAGGCAGCAAACGATGCCGGATTTCACCTGGCGGt < 1:3259570/68‑1 (MQ=255) tATCCGTTTGGCGGATTTAATGACAACGCCGTGAAGGCAGCAAACGATGCCGGATTTCACCTGGCGGt < 1:235339/68‑1 (MQ=255) tATCCGTTTGGCGGATTTAATGACAACGCCGTGAAGGCAGCAAACGATGCCGGATTTCACCTGGCGGt < 1:2270258/68‑1 (MQ=255) tCCGTTTGGCGGATTTAATGACAACGCCGTGAAGTCAGCAAACGATGCCGGATTTCACCTGGCGGTGAc > 1:157054/1‑69 (MQ=255) tCCGTTTGGCGGATTTAATGACAACGCCGTGAAGGCAGCAAACGATGCCGGATTTCACCTGGCGGTGAc > 1:950616/1‑69 (MQ=255) tCCGTTTGGCGGATTTAATGACAACGCCGTGAAGGCAGCAAACGATGCCGGATTTCACCTGGCGGTGAc > 1:30992/1‑69 (MQ=255) tCCGTTTGGCGGATTTAATGACAACGCCGTGAAGGCAGCAAACGATGCCGGATTTCACCTGGCGGTGAc > 1:3375636/1‑69 (MQ=255) tCCGTTTGGCGGATTTAATGACAACGCCGTGAAGGCAGCAAACGATGCCGGATTTCACCTGGCGGTGAc > 1:764800/1‑69 (MQ=255) tCCGTTTGGCGGATTTAATGACAACGCCGTGAAGGCAGCAAACGATGCCGGATTTCACCTGGCGGTGAc > 1:791787/1‑69 (MQ=255) tCCGTTTGGCGGATTTAATGACAACGCCGTGAAGGCAGCAAACGATGCCGGATTTCACCTGGCGGTGAc > 1:92593/1‑69 (MQ=255) tCCGTTTGGCGGATTTAATGACAACGCCGTGAAGGCAGCAAACGATGCCGGATTTCACCTGGCGGTGAc > 1:2249760/1‑69 (MQ=255) tCCGTTTGGCGGATTTAATGACAACGCCGTGAAGGCAGCAAACGATGCCGGATTTCACCTGGCGGTGAc > 1:1030383/1‑69 (MQ=255) tCCGTTTGGCGGATTTAATGACAACGCCGTGAAGGCAGCAAACGATGCCGGATTTCACCTGGCGGTGAc > 1:1143291/1‑69 (MQ=255) tCCGTTTGGCGGATTTAATGACAACGCCGTGAAGGCAGCAAACGATGCCGGATTTCACCTGGCGGTGAc > 1:1279945/1‑69 (MQ=255) tCCGTTTGGCGGATTTAATGACAACGCCGTGAAGGCAGCAAACGATGCCGGATTTCACCTGGCGGTGAc > 1:2180152/1‑69 (MQ=255) cGTTTGGCGGATTTAATGACAACGCCGTGAAGGCAGCAAACGATGCCGGATTTCACCTGGCGGTGACaa < 1:1019291/69‑1 (MQ=255) cGTTTGGCGGATTTAATGACAACGCCGTGAAGGCAGCAAACGATGCCGGATTTCACCTGGCGGTGACaa < 1:2205757/69‑1 (MQ=255) cGTTTGGCGGATTTAATGACAACGCCGTGAAGGCAGCAAACGATGCCGGATTTCACCTGGCGGTGACaa < 1:2521120/69‑1 (MQ=255) cGTTTGGCGGATTTAATGACAACGCCGTGAAGGCAGCAAACGAAGCCGGATTTCACCTGGCGGCGACaa < 1:2245321/69‑1 (MQ=255) tttGGCGGATTTAATGACAACGCCGTGAAGGCAGCAAACGATGCCGGATTTCACCTGGCGGTGACAAc < 1:1382620/68‑1 (MQ=255) tttGGCGGATTTAATGACAACGCCGTGAAGGCAGCAAACGATGCCGGATTTCACCTGGCGGTGACAAc < 1:2780842/68‑1 (MQ=255) tttGGCGGATTTAATGACAACGCCGTGAAGGCAGCAAACGATGCCGGATTTCACCTGGCGGTGACAAc < 1:706514/68‑1 (MQ=255) tttGGCGGATTTAATGACAACGCCGTGAAGGCAGCAAACGATGCCGGATTTCACCTGGCGGTGACAAc < 1:124807/68‑1 (MQ=255) tttGGCGGATTTAATGACAACGCCGTGAAGGCAGCAAACGATGCCGGATTTCACCTGGCGGTGACAAc < 1:2248090/68‑1 (MQ=255) tttAATGACAACGCCGTGAAGGCAGCAAACGATGCCGGATTTCACCTGGCGGTGACAACCATGAAAgg > 1:762595/1‑68 (MQ=255) tttAATGACAACGCCGTGAAGGCAGCAAACGATGCCGGATTTCACCTGGCGGTGACAACCATGAAAgg > 1:2636153/1‑68 (MQ=255) tAATGACAAAGCCGTGAAGGCAGCAAAAGAAGCCGGATTTCACCTGGCGGTGAcg < 1:1206525/55‑2 (MQ=255) tAATGACAAAGCCGTGAAGGCAGCAAAAGAAGCCGGATTTCACCTGGCGGTGAcg < 1:1356976/55‑2 (MQ=255) tAATGACAAAGCCGTGAAGGCAGCAAAAGAAGCCGGATTTCACCTGGCGGTGAcg < 1:1556177/55‑2 (MQ=255) tAATGACAAAGCCGTGAAGGCAGCAAAAGAAGCCGGATTTCACCTGGCGGTGAcg < 1:2777358/55‑2 (MQ=255) tAATGACAAAGCCGTGAAGGCAGCAAAAGAAGCCGGATTTCACCTGGCGGTGAcg < 1:2251176/55‑2 (MQ=255) aCAACGCCGTGAAGGCAGCAAACGATGCCGGATTTCACCTGGCGGTGACAACCATGAAAGGCAAAGTaa < 1:1377745/69‑1 (MQ=255) aCAACGCCGTGAAGGCAGCAAACGATGCCGGATTTCACCTGGCGGTGACAACCATGAAAGGCAAAGTaa < 1:1296121/69‑1 (MQ=255) aCAACGCCGTGAAGGCAGCAAACGATGCCGGATTTCACCTGGCGGTGACAACCATGAAAGGCAAAGTaa < 1:424192/69‑1 (MQ=255) aCAACGCCGTGAAGGCAGCAAACGATGCCGGATTTCACCTGGCGGTGACAACCATGAAAGGCAAAGTaa < 1:2601795/69‑1 (MQ=255) cAACGCCGTGAAGGCAGCAAACGATGCCGGATTTCACCTGGCGGTGACAACCATGAAAGGCAAAGTaa < 1:2916779/68‑1 (MQ=255) cAACGCCGTGAAGGCAGCAAACGATGCCGGATTTCACCTGGCGGTGACAACCATGAAAGGCAAAGTaa < 1:1523372/68‑1 (MQ=255) gCCGTGAAGGCAGCAAACGATGCCGGATTTCACCTGGCGGTGACAACCATGAAAGGCAAAGTAAAACCg > 1:1909650/1‑69 (MQ=255) gCCGTGAAGGCAGCAAACGATGCCGGATTTCACCTGGCGGTGACAACCATGAAAGGCAAAGTAAAACCg > 1:1070268/1‑69 (MQ=255) gCCGTGAAGGCAGCAAACGATGCCGGATTTCACCTGGCGGTGACAACCATGAAAGGCAAAGTAAAACCg > 1:451155/1‑69 (MQ=255) gCCGTGAAGGCAGCAAACGATGCCGGATTTCACCTGGCGGTGACAACCATGAAAGGCAAAGTAAAACCg > 1:2405900/1‑69 (MQ=255) gCCGTGAAGGCAGCAAACGATGCCGGATTTCACCTGGCGGTGACAACCATGAAAGGCAAAGTAAAACCg > 1:2152381/1‑69 (MQ=255) gCCGTGAAGGCAGCAAACGATGCCGGATTTCACCTGGCGCTGACAACCATGAAAGGCAAAGTAAAACCg > 1:2073773/1‑69 (MQ=255) gTGAAGGCAGCAAAAGAAGCCGGATTTCACCTGGCGGTGAcg < 1:464422/42‑2 (MQ=38) aaGGCAGCAAACGATGCCGGATTTCACCTGGCGGTGACAACCATGAAAGGCAAAGTAAAACCGGGGGAt > 1:2592975/1‑69 (MQ=255) aaGGCAGCAAACGATGCCGGATTTCACCTGGCGGTGACAACCATGAAAGGCAAAGTAAAACCGGGGGAt > 1:2272404/1‑69 (MQ=255) aaGGCAGCAAACGATGCCGGATTTCACCTGGCGGTGACAACCATGAAAGGCAAAGTAAAACCGGGGGAt > 1:2258520/1‑69 (MQ=255) agcaAACGATGCCGGATTTCACCTGGCGGTGACAAc < 1:2080894/36‑1 (MQ=255) aCGATGCCGGATTTCACCTGGCGGTGACAACCATGAAAGGCAAAGTAAAACCGGGGGATAATCCgttgt < 1:3350164/69‑1 (MQ=255) | ATCTTTCGTATCCGTTTGGCGGATTTAATGACAACGCCGTGAAGGCAGCAAACGATGCCGGATTTCACCTGGCGGTGACAACCATGAAAGGCAAAGTAAAACCGGGGGATAATCCGTTGT > minE/130851‑130970 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |