| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,714,472 | G→C | 32.0% | A320A (GCC→GCG) | yphG ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,714,472 | 0 | G | C | 32.0% | 114.8 / 73.4 | 103 | A320A (GCC→GCG) | yphG | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (35/35); new base C (22/11); total (57/46) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.39e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01 | |||||||||||
Only 100 of 103 total aligned reads displayed.
TACTGCTCAAAACGCTTCTCTTCGTAAGCATCAAGCCAGGTAAAATCGGGCTGGTTATCGGCAAAAATACCGGTCATCAGTTCGATATACGGGCCGTTATTGTCGGTCA > minE/1714413‑1714521 | tACTGCTCAAAACGCTTCTCTTCGTAAGCATCAAGCCAGGTAAAATCGGGCTGTTTATCGGCaaaaa < 1:2214775/67‑1 (MQ=255)tACTGCTCAAAACGCTTCTCTTCGTAAGCATCAAGCCAGGTAAAATCGGGCTGGTTATCGGCaaaaa < 1:791374/67‑1 (MQ=255)tACTGCTCAAAACGCTTCTCTTCGTAAGCATCAAGCCAGGTAAAATCGGGCTGGTTATCGGCaaaaa < 1:1359580/67‑1 (MQ=255) aCTGCTCAAAACGCTTCTCTTCGTAAGCATCAAGCCAGGTAAAATCGGGCTGGTTATCGGCAAAAATAc > 1:958396/1‑69 (MQ=255) aCTGCTCAAAACGCTTCTCTTCGTAAGCATCAAGCCAGGTAAAATCGGGCTGGTTATCGGCAAAAATAc > 1:89668/1‑69 (MQ=255) aCTGCTCAAAACGCTTCTCTTCGTAAGCATCAAGCCAGGTAAAATCGGGCTGGTTATCGGCAAAAATAc > 1:427593/1‑69 (MQ=255) aCTGCTCAAAACGCTTCTCTTCGTAAGCATCAAGCCAGGTAAAATCGGGCTGGTTATCGGCAAAAATAc > 1:114479/1‑69 (MQ=255) aCTGCTCAAAACGCTTCTCTTCGTAAGCATCAAGCCAGGTAAAATCGGGCTGGTTATCGGCAAAAATAc > 1:598713/1‑69 (MQ=255) aCTGCTCAAAACGCTTCTCTTCGTAAGCATCAAGCCAGGTAAAATCGAGCTGGTTATCGGCAAAAATAc > 1:1156137/1‑69 (MQ=255) cAAAACGCTTCTCTTCGTAAGCATCAAGCCAGGTAAAATCGGGCTGGTTATCGGCAAAAATACCGGt < 1:2470035/67‑1 (MQ=255) cAAAACGCTTCTCTTCGTAAGCATCAAGCCAGGTAAAATCGGGCTGGTTATCGGCAAAAATACCGGt < 1:1576970/67‑1 (MQ=255) cAAAACGCTTCTCTTCGTAAGCATCAAGCCAGGTAAAATCGGGCTGGTTATCGGCAAAAATACCGGt < 1:1236682/67‑1 (MQ=255) aCGCTTCTCTTCGTAAGCATCAAGCCAGGTAAAATCGGGCTGGTTATCGGCAAAAATACCGGTCATCAg > 1:1456754/1‑69 (MQ=255) aCGCTTCTCTTCGTAAGCATCAAGCCAGGTAAAATCGGGCTGGTTATCGGCAAAAATACCGGTCATCAg > 1:345126/1‑69 (MQ=255) aCGCTTCTCTTCGTAAGCATCAAGCCAGGTAAAATCGGGCTGGTTATCGGCAAAAATACCGGTCATCAg > 1:664840/1‑69 (MQ=255) aCGCTTCTCTTCGTAAGCATCAAGCCAGGTAAAATCGGGCTGGTTATCGGCAAAAATACCGGTCATCAg > 1:969390/1‑69 (MQ=255) cGCTTCTCTTCATAAGCATCAAGCCAGGTAAAATCGGGCTGATTATCCGCAAAAATGCCGGTCATCAGt < 1:68990/69‑1 (MQ=255) cGCTTCTCTTCATAAGCATCAAGCCAGGTAAAATCGGGCTGATTATCCGCAAAAATGCCGGTCATCAGt < 1:106402/69‑1 (MQ=255) cGCTTCTCTTCATAAGCATCAAGCCAGGTAAAATCGGGCTGATTATCCGCAAAAATGCCGGTCATCAGt < 1:2508572/69‑1 (MQ=255) cGCTTCTCTTCATAAGCATCAAGCCAGGTAAAATCGGGCTGATTATCCGCAAAAATGCCGGTCATCAGt < 1:1000291/69‑1 (MQ=255) cGCTTCTCTTCATAAGCATCAAGCCAGGTAAAATCGGGCTGATTATCCGCAAAAATGCCGGTCATCAGt < 1:3230018/69‑1 (MQ=255) cGCTTCTCTTCATAAGCATCAAGCCAGGTAAAATCGGGCTGATTATCCGCAAAAATGCCGGTCATCAGt < 1:3346326/69‑1 (MQ=255) cGCTTCTCTTCATAAGCATCAAGCCAGGTAAAATCGGGCTGATTATCCGCAAAAATGCCGGTCATCAGt < 1:904669/69‑1 (MQ=255) cGCTTCTCTTCATAAGCATCAAGCCAGGTAAAATCGGGCTGATTATCCGCAAAAATGCCGGTCATCAGt < 1:653889/69‑1 (MQ=255) cGCTTCTCTTCATAAGCATCAAGCCAGGTAAAATCGGGCTGATTATCCGCAAAAATGCCGGTCATCAGt < 1:1389009/69‑1 (MQ=255) gCTTCTCTTCATAAGCATCAAGCCAGGTAAAATCGGGCTGATTATCCGCAAAAATGCCGGTCATCAGt < 1:1628954/68‑1 (MQ=255) tctcTTCGTAAGCATCAAGCCAGGTAAAATCGGGCTGGTTATCGGCAAAAATACCGGTCATCAGTTCGa < 1:30150/69‑1 (MQ=255) tctcTTCGTAAGCATCAAGCCAGGTAAAATCGGGCTGGTTATCGGCAAAAATACCGGTCATCAGTTCGa < 1:2131507/69‑1 (MQ=255) tctcTTCGTAAGCATCAAGCCAGGTAAAATCGGGCTGGTTATCGGCAAAAATACCGGTCATCAGTTCGa < 1:2359782/69‑1 (MQ=255) ctTCGTAAGCATCAAGCCAGGTAAAATCGGGCTGGTTATCGGCAAAAATACCGGTCATCAGTTCGata < 1:813179/68‑1 (MQ=255) ctTCGTAAGCATCAAGCCAGGTAAAATCGGGCTGGTTATCGGCAAAAATACCGGTCATCAGTTCGata < 1:78212/68‑1 (MQ=255) ctTCGTAAGCATCAAGCCAGGTAAAATCGGGCTGGTTATCGGCAAAAATACCGGTCATCAGTTCGata < 1:2948397/68‑1 (MQ=255) ctTCGTAAGCATCAAGCCAGGTAAAATCGGGCTGGTTATCGGCAAAAATACCGGTCATCAGTTCGata < 1:293229/68‑1 (MQ=255) ctTCGTAAGCATCAAGCCAGGTAAAATCGGGCTGGTTATCGGCAAAAATACCGGTCATCAGTTCGata < 1:2829794/68‑1 (MQ=255) ctTCGTAAGCATCAAGCCAGGTAAAATCGGGCTGGTTATCGGCAAAAATACCGGTCATCAGTTCGata < 1:694656/68‑1 (MQ=255) ctTCGTAAGCATCAAGCCAGGTAAAATCGGGCTGGTTATCGGCAAAAATACCGGTCATCAGTTCGata < 1:2655909/68‑1 (MQ=255) ctTCGTAAGCATCAAGCCAGGTAAAATCGGGCTGGTTATCGGCAAAAATACCGGTCATCAGTTCGata < 1:2339830/68‑1 (MQ=255) ctTCGTAAGCATCAAGCCAGGTAAAATCGGGCTGGTTATCGGCAAAAATACCGGTCATCAGTTCGata < 1:1948379/68‑1 (MQ=255) ctTCGTAAGCATCAAGCCAGGTAAAATCGGGCTGGTTATCGGCAAAAATACCGGTCATCAGTTCGata < 1:1110059/68‑1 (MQ=255) ctTCGTAAGCATCAAGCCAGGTAAAATCGGGCTGGTTATCGGCAAAAATACCGGTCATCAGTTCGata < 1:2145972/68‑1 (MQ=255) ctTCGTAAGCATCAAGCCAGGTAAAATCGGGCTGGTTATCGGCAAAAATACCGGTCATCAGTTCGata < 1:2085733/68‑1 (MQ=255) gCATCAAGCCAGGTAAAATCGGGCTGATTATCCGCaaaa > 1:1382836/1‑39 (MQ=38) gCATCAAGCCAGGTAAAATCGGGCTGATTATCCGCaaaa > 1:2986087/1‑39 (MQ=38) gCATCAAGCCAGGTAAAATCGGGCTGATTATCCGCaaaa > 1:2999099/1‑39 (MQ=38) gCATCAAGCCAGGTAAAATCGGGCTGATTATCCGCaaaa > 1:1837242/1‑39 (MQ=38) gCATCAAGCCAGGTAAAATCGGGCTGATTATCCGCaaaa > 1:3106790/1‑39 (MQ=38) gCATCAAGCCAGGTAAAATCGGGCTGATTATCCGCaaaa > 1:1756369/1‑39 (MQ=38) gCATCAAGCCAGGTAAAATCGGGCTGATTATCCGCaaaa > 1:1745223/1‑39 (MQ=38) gCATCAAGCCAGGTAAAATCGGGCTGATTATCCGCaaaa > 1:1710080/1‑39 (MQ=38) gCATCAAGCCAGGTAAAATCGGGCTGATTATCCGCaaaa > 1:538607/1‑39 (MQ=38) gCATCAAGCCAGGTAAAATCGGGCTGATTATCCGCaaaa > 1:1568447/1‑39 (MQ=38) gCATCAAGCCAGGTAAAATCGGGCTGATTATCCGCaaaa > 1:2306156/1‑39 (MQ=38) gCATCAAGCCAGGTAAAATCGGGCTGATTATCCGCaaaa > 1:1226374/1‑39 (MQ=38) gCATCAAGCCAGGTAAAATCGGGCTGATTATCCGCaaaa > 1:702331/1‑39 (MQ=38) gCATCAAGCCAGGTAAAATCGGGCTGATTATCCGCaaaa > 1:1208382/1‑39 (MQ=38) gCATCAAGCCAGGTAAAATCGGGCTGATTATCCGCaaaa > 1:875375/1‑39 (MQ=38) gCATCAAGCCAGGTAAAATCGGGCTGATTATCCGCaaaa > 1:1097920/1‑39 (MQ=38) gCATCAAGCCAGGTAAAATCGGGCTGATTATCCGCaaaa > 1:93545/1‑39 (MQ=38) gCATCAAGCCAGGTAAAATCGGGCTGATTATCCGCaaaa > 1:969029/1‑39 (MQ=38) gCATCAAGCCAGGTAAAATCGGGCTGATTATCCGCaaaa > 1:2895806/1‑39 (MQ=38) gCATCAAGCCAGGTAAAATCGGGCTGATTATCCGCaaaa > 1:2012901/1‑39 (MQ=38) gCATCAAGCCAGGTAAAATCGGGCTGATTATCCGCaaaa > 1:2774205/1‑39 (MQ=38) cAAGCCAGGTAAAATCGGGCTGGTTATCGGCAAAAATACCGGTCATCAGTTCGATATACGGGCCGTTAt > 1:2495712/1‑69 (MQ=255) cAAGCCAGGTAAAATCGGGCTGGTTATCGGCAAAAATACCGGTCATCAGTTCGATATACGGGCCGTTAt > 1:35712/1‑69 (MQ=255) aGGTAAAATCGGGCTGATTATCCGCAAAAATGCCGGTCATCAGt < 1:644028/44‑1 (MQ=38) tAAAATCGGGCTGGTTATCGGCAAAAATACCGGTCATCAGTTCGATATACGGGCCGTTATTGTCGGTc > 1:2778877/1‑68 (MQ=255) tAAAATCGGGCTGGTTATCGGCAAAAATACCGGTCATCAGTTCGATATACGGGCCGTTATTGTCGGTCa < 1:1044904/69‑1 (MQ=255) tAAAATCGGGCTGGTTATCGGCAAAAATACCGGTCATCAGTTCGATATACGGGCCGTTATTGTCGGTCa < 1:2183025/69‑1 (MQ=255) tAAAATCGGGCTGGTTATCGGCAAAAATACCGGTCATCAGTTCGATATACGGGCCGTTATTGTCGGTCa > 1:1629877/1‑69 (MQ=255) tAAAATCGGGCTGGTTATCGGCAAAAATACCGGTCATCAGTTCGATATACGGGCCGTTATTGTCGGTCa < 1:371300/69‑1 (MQ=255) tAAAATCGGGCTGGTTATCGGCAAAAATACCGGTCATCAGTTCGATATACGGGCCGTTATTGTCGGTCa < 1:347830/69‑1 (MQ=255) tAAAATCGGGCTGGTTATCGGCAAAAATACCGGTCATCAGTTCGATATACGGGCCGTTATTGTCGGTCa < 1:2696645/69‑1 (MQ=255) tAAAATCGGGCTGGTTATCGGCAAAAATACCGGTCATCAGTTCGATATACGGGCCGTTATTGTCGGTCa < 1:3378969/69‑1 (MQ=255) tAAAATCGGGCTGGTTATCGGCAAAAATACCGGTCATCAGTTCGATATACGGGCCGTTATTGTCGGTCa < 1:2347840/69‑1 (MQ=255) tAAAATCGGGCTGGTTATCGGCAAAAATACCGGTCATCAGTTCGATATACGGGCCGTTATTGTCGGTCa > 1:3350809/1‑69 (MQ=255) tAAAATCGGGCTGGTTATCGGCAAAAATACCGGTCATCAGTTCGATATACGGGCCGTTATTGTCGGTCa > 1:2195878/1‑69 (MQ=255) aaaaTCGGGCTGGTTATCGGCAAAAATACCGGtcatc > 1:2320531/1‑37 (MQ=255) aaaaTCGGGCTGGTTATCGGCAAAAATACCGGtcatc > 1:82969/1‑37 (MQ=255) aaaaTCGGGCTGGTTATCGGCAAAAATACCGGtcatc > 1:104687/1‑37 (MQ=255) aaaaTCGGGCTGGTTATCGGCAAAAATACCGGtcatc > 1:1143350/1‑37 (MQ=255) aaaaTCGGGCTGGTTATCGGCAAAAATACCGGtcatc > 1:2439403/1‑37 (MQ=255) aaaaTCGGGCTGGTTATCGGCAAAAATACCGGtcatc > 1:738495/1‑37 (MQ=255) aaaaTCGGGCTGGTTATCGGCAAAAATACCGGtcatc > 1:2289827/1‑37 (MQ=255) aaaaTCGGGCTGGTTATCGGCAAAAATACCGGtcatc > 1:2057332/1‑37 (MQ=255) aaaaTCGGGCTGGTTATCGGCAAAAATACCGGtcatc > 1:2526185/1‑37 (MQ=255) aaaaTCGGGCTGGTTATCGGCAAAAATACCGGtcatc > 1:1398686/1‑37 (MQ=255) aaaaTCGGGCTGGTTATCGGCAAAAATACCGGtcatc > 1:14500/1‑37 (MQ=255) aaaaTCGGGCTGGTTATCGGCAAAAATACCGGtcatc > 1:455092/1‑37 (MQ=255) aaaaTCGGGCTGGTTATCGGCAAAAATACCGGtcatc > 1:3341423/1‑37 (MQ=255) aaaaTCGGGCTGGTTATCGGCAAAAATACCGGtcat > 1:3155131/1‑36 (MQ=255) aaaaTCGGGCTGGTTATCGGCAAAAATACCGGTCATCAGTTCGATATACGGGCCGTTATTGTCGGTCa < 1:2477495/68‑1 (MQ=255) aaaaTCGGGCTGGTTATCGGCAAAAATACCGGTCATCAGTTCGATATACGGGCCGTTATTGTCGGTCa > 1:2560445/1‑68 (MQ=255) aaaaTCGGGCTGGTTATCGGCAAAAATACCGGTCATCAGTTCGATATACGGGCCGTTATTGTCGGTCa > 1:565103/1‑68 (MQ=255) aaaaTCGGGCTGGTTATCGGCAAAAATACCGGTCATCAGTTCGATATACGGGCCGTTATTGTCGGTCa > 1:2969252/1‑68 (MQ=255) aaaaTCGGGCTGGTTATCGGCAAAAATACCGGTCATCAGTTCGATATACGGGCCGTTATTGTCGGTCa < 1:3376224/68‑1 (MQ=255) aaaaTCGGGCTGGTTATCGGCAAAAATACCGGTCATCAGTTCGATATACGGGCCGTTATTGTCGGTCa < 1:1074049/68‑1 (MQ=255) aaaaTCGGGCTGGTTATCGGCAAAAATACCGGTCATCAGTTCGATATACGGGCCGTTATTGTCGGTCa > 1:2939356/1‑68 (MQ=255) aaaaTCGGGCTGGTTATCGGCAAAAATACCGGTCATCAGTTCGATATACGGGCCGTTATTGTCGGTCa > 1:2973069/1‑68 (MQ=255) aaaTCGGTCTGGTTATCGGCAAAAATACCGGTCATCAGTTCGata < 1:527497/45‑1 (MQ=255) gCTGGTTATCGGCAAAAATACCGGTCATCAGTTCGa < 1:1871640/36‑1 (MQ=255) | TACTGCTCAAAACGCTTCTCTTCGTAAGCATCAAGCCAGGTAAAATCGGGCTGGTTATCGGCAAAAATACCGGTCATCAGTTCGATATACGGGCCGTTATTGTCGGTCA > minE/1714413‑1714521 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |