| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,714,688 | A→G | 28.3% | P248P (CCT→CCC) | yphG ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,714,688 | 0 | A | G | 28.3% | 104.0 / 44.2 | 102 | P248P (CCT→CCC) | yphG | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (47/26); new base G (18/11); total (65/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.24e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01 | |||||||||||
Only 100 of 102 total aligned reads displayed.
TTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGTCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGTGCC > minE/1714622‑1714750 | ttCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAgg > 1:2732606/1‑69 (MQ=255)ttCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAgg > 1:1739830/1‑69 (MQ=255)ttCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAgg > 1:17197/1‑69 (MQ=255)ttCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAgg > 1:472213/1‑69 (MQ=255)ttCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAgg > 1:1657258/1‑69 (MQ=255)ttCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAgg > 1:232960/1‑69 (MQ=255)ttCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAgg > 1:549886/1‑69 (MQ=255)ttCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAgg > 1:2000970/1‑69 (MQ=255)ttCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAgg > 1:306699/1‑69 (MQ=255)ttCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAgg > 1:1468153/1‑69 (MQ=255)ttCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAgg > 1:1423208/1‑69 (MQ=255)ttCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAgg > 1:1411147/1‑69 (MQ=255)ttCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAgg > 1:61111/1‑69 (MQ=255)ttCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAgg > 1:2855101/1‑69 (MQ=255)ttCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAgg > 1:2471894/1‑69 (MQ=255)ttCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAgg > 1:2502115/1‑69 (MQ=255)ttCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAgg > 1:232225/1‑69 (MQ=255)ttCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAgg > 1:21837/1‑69 (MQ=255)ttCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAgg > 1:846893/1‑69 (MQ=255)ttCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAgg > 1:1178637/1‑69 (MQ=255)ttCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAgg > 1:923765/1‑69 (MQ=255)ttCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAg > 1:2279018/1‑68 (MQ=255) gTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACAtt > 1:3023937/1‑69 (MQ=255) gTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACAtt > 1:3085563/1‑69 (MQ=255) gTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACAtt > 1:2869558/1‑69 (MQ=255) gTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACAtt > 1:2329983/1‑69 (MQ=255) gTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACAtt > 1:3336514/1‑69 (MQ=255) gTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACAtt > 1:1810140/1‑69 (MQ=255) gTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACAtt > 1:532447/1‑69 (MQ=255) gTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACAtt > 1:532941/1‑69 (MQ=255) gTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACAtt > 1:2448936/1‑69 (MQ=255) gTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACAtt > 1:1272888/1‑69 (MQ=255) gTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACAtt > 1:2767456/1‑69 (MQ=255) gTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACAt > 1:2857099/1‑68 (MQ=255) gACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGATGTTGGAACAGGCACAtttt < 1:240498/69‑1 (MQ=255) gACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACAtttt < 1:282101/69‑1 (MQ=255) gACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACAtttt < 1:2788344/69‑1 (MQ=255) gACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACAtttt < 1:2705814/69‑1 (MQ=255) gACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACAtttt < 1:1794236/69‑1 (MQ=255) gACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACAtttt < 1:1106139/69‑1 (MQ=255) acacCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACAtttt < 1:771276/68‑1 (MQ=255) acGCGCCAATAAAATCGTACTGTGATTTTTCAGCCATATATGAGGTTGGAACGGGCACATTTTtata > 1:1977270/1‑67 (MQ=255) acGCGCCAACAAAATCGTACTGTGATTTTTCAGCCATATATGAGGTTGGAACGGGCACATTTTtata > 1:2880301/1‑67 (MQ=255) acGCGCCAACAAAATCGTACTGTGATTTTTCAGCCATATATGAGGTTGGAACGGGCACATTTTtata > 1:3329342/1‑67 (MQ=255) acGCGCCAACAAAATCGTACTGTGATTTTTCAGCCATATATGAGGTTGGAACGGGCACATTTTtata > 1:552673/1‑67 (MQ=255) acGCGCCAACAAAATCGTACTGTGATTTTTCAGCCATATATGAGGTTGGAACGGGCACATTTTtata > 1:566447/1‑67 (MQ=255) acGCGCCAACAAAATCGTACTGTGATTTTTCAGCCATATATGAGGTTGGAACGGGCACATTTTtata > 1:670013/1‑67 (MQ=255) acGCGCCAACAAAATCGTACTGTGATTTTTCAGCCATATATGAGGTTGGAACGGGCACATTTTtata > 1:733161/1‑67 (MQ=255) acGCGCCAACAAAATCGTACTGTGATTTTTCAGCCATATATGAGGTTGGAACGGGCACATTTTtata > 1:740873/1‑67 (MQ=255) acGCGCCAACAAAATCGTACTGTGATTTTTCAGCCATATATGAGGTTGGAACGGGCACATTTTtata > 1:817156/1‑67 (MQ=255) acGCGCCAACAAAATCGTACTGTGATTTTTCAGCCATATATGAGGTTGGAACGGGCACATTTTtata > 1:958759/1‑67 (MQ=255) acGCGCCAACAAAATCGTACTGTGATTTTTCAGCCATATATGAGGTTGGAACGGGCACATTTTtata > 1:1098904/1‑67 (MQ=255) acGCGCCAACAAAATCGTACTGTGATTTTTCAGCCATATATGAGGTTGGAACGGGCACATTTTtata > 1:1476131/1‑67 (MQ=255) acGCGCCAACAAAATCGTACTGTGATTTTTCAGCCATATATGAGGTTGGAACGGGCACATTTTtata > 1:1345578/1‑67 (MQ=255) acGCGCCAACAAAATCGTACTGTGATTTTTCAGCCATATATGAGGTTGGAACGGGCACATTTTtata > 1:2564416/1‑67 (MQ=255) acGCGCCAACAAAATCGTACTGTGATTTTTCAGCCATATATGAGGTTGGAACGGGCACATTTTtata > 1:1324285/1‑67 (MQ=255) acGCGCCAACAAAATCGTACTGTGATTTTTCAGCCATATATGAGGTTGGAACGGGCACATTTTtata > 1:1318878/1‑67 (MQ=255) acGCGCCAACAAAATCGTACTGTGATTTTTCAGCCATATATGAGGTTGGAACGGGCACATTTTtata > 1:231189/1‑67 (MQ=255) acGCGCCAACAAAATCGTACTGTGATTTTTCAGCCATATATGAGGTTGGAACGGGCACATTTTtat > 1:1957994/1‑66 (MQ=255) gcCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACATTTTTATAGCgaga < 1:1078737/69‑1 (MQ=255) gcCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACATTTTTATAGCgaga < 1:1698581/69‑1 (MQ=255) gcCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACATTTTTATAGCgaga < 1:1230460/69‑1 (MQ=255) gcCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACATTTTTATAGCgaga < 1:1109304/69‑1 (MQ=255) gACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACATTTTTATAGCGAGaaa < 1:345687/68‑1 (MQ=255) cGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGTCCAc > 1:2863917/1‑67 (MQ=255) cGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGTCCAc > 1:125187/1‑67 (MQ=255) cGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGTCCAc > 1:678563/1‑67 (MQ=255) cGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGTCCAc > 1:1384394/1‑67 (MQ=255) tcagCCATATATGAGGTTGGAACGGGCACATTTTTATAGCGAGAAATGTCCACTCCGGCGGagtagt < 1:3042099/64‑1 (MQ=255) ccATATATGAGGTTGGAACGGGCACATTTTTATAGCGAGAAATGTCCACTCCGGCGGagtagt < 1:66426/63‑1 (MQ=255) ccATATATGAGGTTGGAACGGGCACATTTTTATAGCGAGAAATGTCCACTCCGGCGGagtagt < 1:920458/63‑1 (MQ=255) ccATATATGAGGTTGGAACGGGCACATTTTTATAGCGAGAAATGTCCACTCCGGCGGagtagt < 1:1581778/63‑1 (MQ=255) ccATATATGAGGTTGGAACGGGCACATTTTTATAGCGAGAAATGTCCACTCCGGCGGagtagt < 1:516420/63‑1 (MQ=255) ccATATATGAGGTTGGAACGGGCACATTTTTATAGCGAGAAATGTCCACTCCGGCGGagtagt < 1:2681415/63‑1 (MQ=255) ccATATATGAGGTTGGAACGGGCACATTTTTATAGCGAGAAATGTCCACTCCGGCGGagtagt < 1:1702667/63‑1 (MQ=255) ccATATATGAGGTTGGAACGGGCACATTTTTATAGCGAGAAATGTCCACTCCGGCGGagtagt < 1:2692363/63‑1 (MQ=255) ccATATATGAGGTTGGAACGGGCACATTTTTATAGCGAGAAATGTCCACTCCGGCGGagtagt < 1:1896394/63‑1 (MQ=255) ccATATATGAGGTTGGAACGGGCACATTTTTATAGCGAGAAATGTCCACTCCGGCGGagtagt < 1:2736864/63‑1 (MQ=255) ccATATATGAGGTTGGAACGGGCACATTTTTATAGCGAGAAATGTCCACTCCGGCGGagtagt < 1:2361693/63‑1 (MQ=255) tatGAGGTTGGAAGAGGCACATTTTTATAGCGAGAAATGTCCa > 1:3348468/1‑43 (MQ=255) tatGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGTcc > 1:1963864/1‑42 (MQ=255) tatGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGTCCa > 1:308375/1‑43 (MQ=255) tatGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGTCCa > 1:2101059/1‑43 (MQ=255) tatGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGTCCa > 1:3235097/1‑43 (MQ=255) tatGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGTCCa < 1:3379119/43‑1 (MQ=255) tatGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGTCCa > 1:538439/1‑43 (MQ=255) tatGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGTCCa > 1:3245463/1‑43 (MQ=255) tGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGTCCACTCCAGCGGAGTAGTCCACTTTgtagta > 1:1518239/1‑69 (MQ=255) gTTGGAACAGGCACATTTTTATAGCGAGAAATGTCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGt < 1:1371564/68‑1 (MQ=255) gTTGGAACAGGCACATTTTTATAGCGAGAAATGTCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGt < 1:2096271/68‑1 (MQ=255) gTTGGAACAGGCACATTTTTATAGCGAGAAATGTCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGt < 1:2141621/68‑1 (MQ=255) gTTGGAACAGGCACATTTTTATAGCGAGAAATGTCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGt < 1:3236641/68‑1 (MQ=255) gTTGGAACAGGCACATTTTTATAGCGAGAAATGTCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGt < 1:24624/68‑1 (MQ=255) gTTGGAACAGGCACATTTTTATAGCGAGAAATGTCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGt < 1:26063/68‑1 (MQ=255) gTTGGAACAGGCACATTTTTATAGCGAGAAATGTCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGt < 1:3136364/68‑1 (MQ=255) gTTGGAACAAGCACATTTTTATAGCGAGAAATGTCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGt < 1:844816/68‑1 (MQ=255) tGGAACAGGCACATTTTTATAGCGATAAATGTCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGt < 1:2594455/66‑1 (MQ=255) tGGAACAGGCACATTTTTATAGCGAGAAATGTCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGTGcc < 1:1628469/69‑1 (MQ=255) tGGAACAGGCACATTTTTATAGCGAGAAATGTCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGTGcc < 1:3366550/69‑1 (MQ=255) tGGAACAGGCACATTTTTATAGCGAGAAACGTCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGTGcc < 1:773672/69‑1 (MQ=255) | TTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGTCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGTGCC > minE/1714622‑1714750 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |