| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,716,655 | G→A | 19.0% | T369T (ACG→ACA) | yphH → | predicted DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,716,655 | 0 | G | A | 19.0% | 70.1 / 7.8 | 47 | T369T (ACG→ACA) | yphH | predicted DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base G (15/23); new base A (6/3); total (21/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.63e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.66e-01 | |||||||||||
CTATTATTCGCCAGACAGGATTTAACCCGTTCGACCGCGACGAAGGACCGAGCGTGAAAGCGACGCAAATTGGCTTTGGGCAATTAAGCCGCGCACAACAGGTGCTGGGAATTGGCTATTTGTATG > minE/1716591‑1716716 | cTATTATTCGCCAGACAGGATTTAACCCGTTCGACCGCGACGAAGGACCGAGCGTGAAAGCGACGCaaa > 1:1005068/1‑69 (MQ=255)cTATTATTCGCCAGACAGGATTTAACCCGTTCGACCGCGACGAAGGACCGAGCGTGAAAGCGACGCaaa > 1:2305958/1‑69 (MQ=255)cTATTATTCGCCAGACAGGATTTAACCCGTTCGACCGCGACGAAGGACCGAGCGTGAAAGCGACGCa < 1:1035571/67‑1 (MQ=255)cTATTATTCGCCAGACAGGATTTAACCCGTTCGACCGCGACGAAGGACCGAGCGTGAAAGCGACGCa < 1:3366181/67‑1 (MQ=255)cTATTATTCGCCAGACAGGATTTAACCCGTTCGACCGCGACGAAGGACCGAGCGTGAAAGCGACGCa < 1:3292040/67‑1 (MQ=255)cTATTATTCGCCAGACAGGATTTAACCCGTTCGACCGCGACGAAGGACCGAGCGTGAAAGCGACGCa < 1:1963072/67‑1 (MQ=255) attattCGCCAGACAGGATTTAACCCGTTCGACCGCGACGAAGGACCGAGCGTGAAAGCGACGCAAAtt > 1:742182/1‑69 (MQ=255) attCGCCAGACAGGATTTAACCCGTTCGACCGCGACGAAGGACCGAGCGTGAAAGCGACGCAAATTGGc > 1:927808/1‑69 (MQ=255) attCGCCAGACAGGATTTAACCCGTTCGACCGCGACGAAGGACCGAGCGTGAAAGCGACGCAAATTGGc > 1:3028243/1‑69 (MQ=255) ttAACCCGTTCGACCGCGACGAAGGACCGAGCGTGAAAGCGACGCAAATTgg > 1:949655/1‑52 (MQ=255) ttAACCCGTTCGACCGCGACGAAGGACCGAGCGTGAAAGCGACACAGATTGGCTTTGGGCAATTAAGcc < 1:70427/69‑1 (MQ=255) ttAACCCGTTCGACCGCGACGAAGGACCGAGCGTGAAAGCGACACAGATTGGCTTTGGGCAATTAAGcc < 1:461002/69‑1 (MQ=255) aCCCGTTCGACCGCGACGAAGGACCGAGCGTGAAAGCGACACAGATTGGCTTTGGGCAATTAAGCcgcg > 1:1011330/1‑69 (MQ=255) aCCCGTTCGACCGCGACGAAGGACCGAGCGTGAAAGCGACACAGATTGGCTTTGGGCAATTAAGCcgcg > 1:2860201/1‑69 (MQ=255) aCCCGTTCGACCGCGACGAAGGACCGAGCGTGAAAGCGACACAGATTGGCTTTGGGCAATTAAGCcgcg > 1:3022366/1‑69 (MQ=255) aCCCGTTCGACCGCGACGAAGGACCGAGCGTGAAAGCGACACAGATTGGCTTTGGGCAATTAAGCcgcg > 1:2318740/1‑69 (MQ=255) aCCCGTTCGACCGCGACGAAGGACCGAGCGTGAAAGCGACACAGATTGGCTTTGGGCAATTAAGCcgcg > 1:1968118/1‑69 (MQ=255) aCCCGTTCGACCGCGACGAAGGACCGAGCGTGAAAGCGACACAGATTGGCTTTGGGCAATTAAGCcgcg > 1:3228343/1‑69 (MQ=255) cgacgaAGGACCGAGCGTGAAAGCGACGCAAATTGGCTTTGGGCAATTAAGCCGCGCACAACAGGTGCt < 1:557834/69‑1 (MQ=255) cgacgaAGGACCGAGCGTGAAAGCGACGCAAATTGGCTTTGGGCAATTAAGCCGCGCACAACAGGTGCt < 1:1024784/69‑1 (MQ=255) cgacgaAGGACCGAGCGTGAAAGCGACGCAAATTGGCTTTGGGCAATTAAGCCGCGCACAACAGGTGCt < 1:2759698/69‑1 (MQ=255) ggACCGAGCGTGAAAGCGACGCAAATTGGCTTTGGGCAATTAAGCCGCGCACAACAGGTGCTGGGAAtt > 1:1970750/1‑69 (MQ=255) ggACCGAGCGTGAAAGCGACGCAAATTGGCTTTGGGCAATTAAGCCGCGCACAACAGGTGCTGGGAAtt > 1:3025459/1‑69 (MQ=255) ggACCGAGCGTGAAAGCGACGCAAATTGGCTTTGGGCAATTAAGCCGCGCACAACAGGTGCTGGGAAtt > 1:2872116/1‑69 (MQ=255) gTGAAAGCGACGCAAATTGGCTTTGGGCAATTAAGCCGCGCACAACAGGTGCTGGGAATTGGCTATTTg < 1:975594/69‑1 (MQ=255) tGAAAGCGACGCAAATTGGCTTTGGGCAATTAAGCCGCGCACAACAGGTGCTGGGAATTGGCTATTTGt > 1:2461080/1‑69 (MQ=255) tGAAAGCGACGCAAATTGGCTTTGGGCAATTAAGCCGCGCACAACAGGTGCTGGGAATTGGCTATTTGt > 1:1048635/1‑69 (MQ=255) tGAAAGCGACGCAAATTGGCTTTGGGCAATTAAGCCGCGCACAACAGGTGCTGGGAATTGGCTATTTGt < 1:1129224/69‑1 (MQ=255) tGAAAGCGACGCAAATTGGCTTTGGGCAATTAAGCCGCGCACAACAGGTGCTGGGAATTGGCTATTTGt < 1:1394329/69‑1 (MQ=255) tGAAAGCGACGCAAATTGGCTTTGGGCAATTAAGCCGCGCACAACAGGTGCTGGGAATTGGCTATTTGt < 1:152352/69‑1 (MQ=255) tGAAAGCGACGCAAATTGGCTTTGGGCAATTAAGCCGCGCACAACAGGTGCTGGGAATTGGCTATTTGt < 1:653537/69‑1 (MQ=255) tGAAAGCGACGCAAATTGGCTTTGGGCAATTAAGCCGCGCACAACAGGTGCTGGGAATTGGCTATTTGt < 1:1597186/69‑1 (MQ=255) tGAAAGCGACGCAAATTGGCTTTGGGCAATTAAGCCGCGCACAACAGGTGCTGGGAATTGGCTATTTGt < 1:1831808/69‑1 (MQ=255) tGAAAGCGACGCAAATTGGCTTTGGGCAATTAAGCCGCGCACAACAGGTGCTGGGAATTGGCTATTTGt > 1:1831907/1‑69 (MQ=255) tGAAAGCGACGCAAATTGGCTTTGGGCAATTAAGCCGCGCACAACAGGTGCTGGGAATTGGCTATTTGt > 1:1872070/1‑69 (MQ=255) tGAAAGCGACGCAAATTGGCTTTGGGCAATTAAGCCGCGCACAACAGGTGCTGGGAATTGGCTATTTGt < 1:3160293/69‑1 (MQ=255) tGAAAGCGACGCAAATTGGCTTTGGGCAATTAAGCCGCGCACAACAGGTGCTGGGAATTGGCTATTTGt < 1:2263308/69‑1 (MQ=255) tGAAAGCGACGCAAATTGGCTTTGGGCAATTAAGCCGCGCACAACAGGTGCTGGGAATTGGCTATTTGt > 1:2423284/1‑69 (MQ=255) tGAAAGCGACGCAAATTGGCTTTGGGCAATTAAGCCGCGCACAACAGGTGCTGGGAATTGGCTATTTGt < 1:2961396/69‑1 (MQ=255) tGAAAGCGACGCAAATTGGCTTTGGGCAATTAAGCCGCGCACAACAGGTGCTGGGAATTGGCTATTTGt > 1:2495168/1‑69 (MQ=255) tGAAAGCGACACAGATTGGCTTTGGGCAATTAAGcc < 1:2409667/36‑1 (MQ=37) gAAAGCGACGCAAATTGGGTTTGGGCAATTAAGCCGCGCACAACAGGTGCTGGGAATTGGCTATTTGt < 1:1570509/68‑1 (MQ=255) gAAAGCGACGCAAATTGGCTTTGGGCAATTAAGCCGCGCACAACAGGTGCTGGGAATTGGCTATTTGt < 1:1698604/68‑1 (MQ=255) gAAAGCGACGCAAATTGGCTTTGGGCAATTAAGCCGCGCACAACAGGTGCTGGGAATTGGCTATTTGt < 1:521420/68‑1 (MQ=255) gAAAGCGACGCAAATTGGCTTTGGGCAATTAAGCCGCGCACAACAGGTGCTGGGAATTGGCTATTTGt < 1:847202/68‑1 (MQ=255) aGCGACGCAAATTGGCTTTGGGCAATTAAGCCGCGCACAACAGGTGCTGGGAATTGGCTATTTGTATg < 1:696449/68‑1 (MQ=255) aGCGACGCAAATTGGCTTTGGGCAATTAAGCCGCGCACAACAGGTGCTGGGAATTGGCTATTTGTATg < 1:951730/68‑1 (MQ=255) | CTATTATTCGCCAGACAGGATTTAACCCGTTCGACCGCGACGAAGGACCGAGCGTGAAAGCGACGCAAATTGGCTTTGGGCAATTAAGCCGCGCACAACAGGTGCTGGGAATTGGCTATTTGTATG > minE/1716591‑1716716 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |