| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,717,117 | G→A | 37.7% | T359T (ACC→ACT) | glyA ← | serine hydroxymethyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,717,117 | 0 | G | A | 37.7% | 28.6 / 30.6 | 53 | T359T (ACC→ACT) | glyA | serine hydroxymethyltransferase |
| Reads supporting (aligned to +/- strand): ref base G (18/15); new base A (10/10); total (28/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.83e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.14e-01 | |||||||||||
TTAAAGCCGCGACGGGTAATCGCCGGAGTACCTACACGAATACCGGAGGTCACAAACGGGCTCTTCGGATCGTTCGGTACGCTGTTTTTGTTGACGGTGATGTTAGCACGG > minE/1717070‑1717180 | ttAAAGCCGCGACGGGTAATCGCCGGAGTACCTACACGAATACCGGAGGTCACAAACGGGCTCTTCgg < 1:2613004/68‑1 (MQ=255)ttAAAGCCGCGACGGGTAATCGCCGGAGTACCTACACGAATACCGGAGGTCACAAACGGGCTCTTCgg < 1:2929939/68‑1 (MQ=255)ttAAAGCCGCGACGGGTAATCGCCGGAGTACCTACACGAATACCGGAGGTCACAAACGGGCTCTTCgg < 1:1140520/68‑1 (MQ=255)ttAAAGCCGCGACGGGTAATCGCCGGAGTACCTACACGAATACCGGAGGTCACAAACGGGCTCTTCgg < 1:3161816/68‑1 (MQ=255)ttAAAGCCGCGACGGGTAATCGCCGGAGTACCTACACGAATACCGGAGGTCACAAACGGGCTCTTCgg < 1:281143/68‑1 (MQ=255)ttAAAGCCGCGACGGGTAATCGCCGGAGTACCTACACGAATACCGGAGGTCACAAACGGGCTCTTCgg < 1:1692195/68‑1 (MQ=255)ttAAAGCCGCGACGGGTAATCGCCGGAGTACCTACACGAATACCGGAGGTCACAAACGGGCTCTTCgg < 1:1688211/68‑1 (MQ=255) cGCCGGAGTACCTACACGAATACCGGAGGTCACAAACGGGCTCTTGGGATCGTTCGGTACGCTGtttt < 1:333503/68‑1 (MQ=255) cGCCGGAGTACCTACACGAATACCGGAGGTCACAAACGGGCTCTTCGGATCGTTCGGTACGCTGtttt < 1:959624/68‑1 (MQ=255) cGCCGGAGTACCTACACGAATACCGGAGGTCACAAACGGGCTCTTCGGATCGTTCGGTACGCTGtttt > 1:1397013/1‑68 (MQ=255) cGCCGGAGTACCTACACGAATACCGGAGGTCACAAACGGGCTCTTCGGATCGTTCGGTACGCTGtttt < 1:2892605/68‑1 (MQ=255) cGCCGGAGTACCTACACGAATACCGGAGGTCACAAACGGGCTCTTCGGATCGTTCGGTACGCTGtttt > 1:2805111/1‑68 (MQ=255) cGCCGGAGTACCTACACGAATACCGGAGGTCACAAACGGGCTCTTCGGATCGTTCGGTACGCTGtttt < 1:3262877/68‑1 (MQ=255) cGCCGGAGTACCTACACGAATACCGGAGGTCACAAACGGGCTCTTCGGATCGTTCGGTACGCTGtttt > 1:959988/1‑68 (MQ=255) cGCCGGAGTACCTACACGAATACCGGAGGTCACAAACGGGCTCTTCGGATCGTTCGGTACGCTGtttt > 1:2598444/1‑68 (MQ=255) cGCCGGAGTACCTACACGAATACCGGAGGTCACAAACGGGCTCTTCGGATCGTTCGGTACGCTGtttt < 1:2462427/68‑1 (MQ=255) cGCCGGAGTACCTACACGAATACCGGAGGTCACAAACGGGCTCTTCGGATCGTTCGGTACGCTGtttt > 1:2379304/1‑68 (MQ=255) cGCCGGAGTACCTACACGAATACCGGAAGTCACAAACGGGCTCTTCGGATCGTTCGGTACGCTGtttt < 1:245268/68‑1 (MQ=255) cGCCGGAGTACCTACACGAATACCGGAAGTCACAAACGGGCTCTTCGGATCGTTCGGTACGCTGtttt < 1:303344/68‑1 (MQ=255) cGCCGGAGTACCTACACGAATACCGGAAGTCACAAACGGGCTCTTCGGATCGTTCGGTACGCTGtttt < 1:1253629/68‑1 (MQ=255) cGCCGGAGTACCTACACGAATACCGGAAGTCACAAACGGGCTCTTCGGATCGTTCGGTACGCTGtttt < 1:3419197/68‑1 (MQ=255) cGCCGGAGTACCTACACGAATACCGGAAGTCACAAACGGGCTCTTCGGATCGTTCGGTACGCTGtttt < 1:463377/68‑1 (MQ=255) cGCCGGAGTACCTACACGAATACCGGAAGTCACAAACGGGCTCTTCGGATCGTTCGGTACGCTGtttt < 1:3390310/68‑1 (MQ=255) cGCCGGAGTACCTACACGAATACCGGAAGTCACAAACGGGCTCTTCGGATCGTTCGGTACGCTGtttt < 1:942012/68‑1 (MQ=255) cGCCGGAGTACCTACACGAATACCGGAAGTCACAAACGGGCTCTTCGGATCGTTCGGTACGCTGtttt < 1:904327/68‑1 (MQ=255) cGCCGGAGTACCTACACGAACACCGGAGGTCACAAACGGGCTCTTCGGATCGTTCGGTACGCTGtttt > 1:1367039/1‑68 (MQ=255) agCCGGAGTACCTACACGAATACCGGAAGTCACAAACGGGCTCTTCGGATCGTTCGGTACGCTGtttt < 1:3302296/67‑1 (MQ=255) gCCGGAGTACCTACACGAATACCGGAAGGCACAAACGGGCTCTTCGGATCGTTCGGTACGCTGtttt < 1:2665029/67‑1 (MQ=255) tACCTACACGAATACCGGAGGTCACAAACGGGCTCTTCGGATCGTTCGGTACGCTGtttt < 1:2326399/60‑1 (MQ=255) ccTACACGAATACCGGAGGTCACAAACGGGCTCTTCGGATCGTTCGGt > 1:642231/1‑48 (MQ=255) ccTACACGAATACCGGAGGTCACAAACGGGCTCTTCGGATCGTTCGGTACGCTGTTTTTGTTGACGGTg > 1:3225685/1‑69 (MQ=255) ccTACACGAATACCGGAGGTCACAAACGGGCTCTTCGGATCGTTCGGTACGCTGTTTTTGTTGACGGTg > 1:1008408/1‑69 (MQ=255) cGAATACCGGAGGTCACAAACGGGCTCTTCGGATCGTTCGGTACGCTGTTTTTGTTGACGGt > 1:3414892/1‑62 (MQ=255) gAATACCGGAGGTCTCAAACGGGCTCTTCGGATCGTTCGGTACGCTGtttt < 1:278500/51‑1 (MQ=255) ccGGAGGTCACAAACGGGCTCTTCGGATCGTTCGGTACGCTGtttt < 1:1099304/46‑1 (MQ=255) ccGGAGGTCACAAACGGGCTCTTCGGATCGTTCGGTACGCTGTTTTTGTTGACGGTGATGTTAGCACgg > 1:3193094/1‑69 (MQ=255) ccGGAGGTCACAAACGGGCTCTTCGGATCGTTCGGTACGCTGTTTTTGTTGACGGTGATGTTAGCACgg > 1:2694814/1‑69 (MQ=255) ccGGAGGTCACAAACGGGCTCTTCGGATCGTTCGGTACGCTGTTTTTGTTGACGGTGATGTTAGCACgg > 1:1856404/1‑69 (MQ=255) ccGGAGGTCACAAACGGGCTCTTCGGATCGTTCGGTACGCTGTTTTTGTTGACGGTGATGTTAGCACgg > 1:2628992/1‑69 (MQ=255) ccGGAGGTCACAAACGGGCTCTTCGGATCGTTCGGTACGCTGTTTTTGTTGACGGTGATGTTAGCACgg > 1:2598089/1‑69 (MQ=255) ccGGAGGTCACAAACGGGCTCTTCGGATCGTTCGGTACGCTGTTTTTGTTGACGGTGATGTTAGCACgg > 1:2421611/1‑69 (MQ=255) ccGGAGGTCACAAACGGGCTCTTCGGATCGTTCGGTACGCTGTTTTTGTTGACGGTGATGTTAGCACgg > 1:2171560/1‑69 (MQ=255) ccGGAGGTCACAAACGGGCTCTTCGGATCGTTCGGTACGCTGTTTTTGTTGACGGTGATGATAGCACgg > 1:2451306/1‑69 (MQ=255) cGGAAGTCACAAACGGGCTCTTCGGATCGTTCGGTACGCTGTTTTTGTTCACGGTGATGTTAGCACgg > 1:2298169/1‑68 (MQ=255) cGGAAGTCACAAACGGGCTCTTCGGATCGTTCGGTACGCTGTTTTTGTTCACGGTGATGTTAGCACgg > 1:1016895/1‑68 (MQ=255) cGGAAGTCACAAACGGGCTCTTCGGATCGTTCGGTACGCTGTTTTTGTTCACGGTGATGTTAGCACgg > 1:155033/1‑68 (MQ=255) cGGAAGTCACAAACGGGCTCTTCGGATCGTTCGGTACGCTGTTTTTGTTCACGGTGATGTTAGCACgg > 1:1965979/1‑68 (MQ=255) cGGAAGTCACAAACGGGCTCTTCGGATCGTTCGGTACGCTGTTTTTGTTCACGGTGATGTTAGCACgg > 1:536300/1‑68 (MQ=255) cGGAAGTCACAAACGGGCTCTTCGGATCGTTCGGTACGCTGTTTTTGTTCACGGTGATGTTAGCACgg > 1:2256369/1‑68 (MQ=255) cGGAAGTCACAAACGGGCTCTTCGGATCGTTCGGTACGCTGTTTTTGTTCACGGTGATGTTAGCACgg > 1:3014683/1‑68 (MQ=255) cGGAAGTCACAAACGGGCTCTTCGGATCGTTCGGTACGCTGTTTTTGTTCACGGTGATGTTAGCACgg > 1:2705520/1‑68 (MQ=255) cGGAAGTCACAAACGGGCTCTTCGGATCGTTCGGTACGCTGTTTTTGTTCACGGTGATGTTAGCACgg > 1:2862560/1‑68 (MQ=255) cGGAAGTCACAAACGGGCTCTTCGGATCGTTCGGTACGCTGTTTTTGTTCACGGTGATGTTAGCACgg > 1:2911322/1‑68 (MQ=255) ggTCACTAACGGGCTCTTCGTATCGTTCGGTACGCTGtttt < 1:2199617/41‑1 (MQ=255) | TTAAAGCCGCGACGGGTAATCGCCGGAGTACCTACACGAATACCGGAGGTCACAAACGGGCTCTTCGGATCGTTCGGTACGCTGTTTTTGTTGACGGTGATGTTAGCACGG > minE/1717070‑1717180 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |