| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,725,308 | G→A | 45.4% | F974F (TTC→TTT) | purL ← | phosphoribosylformyl‑glycineamide synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,725,308 | 0 | G | A | 45.4% | 11.6 / 51.3 | 66 | F974F (TTC→TTT) | purL | phosphoribosylformyl‑glycineamide synthetase |
| Reads supporting (aligned to +/- strand): ref base G (22/14); new base A (10/20); total (32/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.91e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.20e-01 | |||||||||||
GCAGGCGCTGCATCTGCCAGGTAGTTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACAAAACGGTCACCGGAAACCGCCTGCCCTACATA > minE/1725244‑1725370 | gCAGGCGCTGCATCTGCCAGGTAGTTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTGAATa < 1:3336221/69‑1 (MQ=255) gTTTCTGCCCACCAGACACGCAACGTGGTGCTGCTTTCGCTAAATACAGTCTGCCCGTTgg < 1:950524/61‑1 (MQ=255) gTTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTAAATACAGTCTGCCCGTTgg < 1:1077318/61‑1 (MQ=255) gTTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTAAATACAGTCTGCCCGTTgg < 1:2186005/61‑1 (MQ=255) gTTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTAAATACAGTCTGCCCGTTgg < 1:2182138/61‑1 (MQ=255) gTTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTAAATACAGTCTGCCCGTTgg < 1:800937/61‑1 (MQ=255) gTTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTAAATACAGTCTGCCCGTTgg < 1:755600/61‑1 (MQ=255) gTTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTAAATACAGTCTGCCCGTTgg < 1:725972/61‑1 (MQ=255) gTTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTAAATACAGTCTGCCCGTTgg < 1:2489550/61‑1 (MQ=255) gTTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTAAATACAGTCTGCCCGTTgg < 1:2711690/61‑1 (MQ=255) gTTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTAAATACAGTCTGCCCGTTgg < 1:1702903/61‑1 (MQ=255) gTTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTAAATACAGTCTGCCCGTTgg < 1:1698302/61‑1 (MQ=255) gTTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTAAATACAGTCTGCCCGTTgg < 1:1686813/61‑1 (MQ=255) gTTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTAAATACAGTCTGCCCGTTgg < 1:1530584/61‑1 (MQ=255) gTTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTAAATACAGTCTGCCCGTTgg < 1:1178656/61‑1 (MQ=255) gTTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTAAATACAGTCTGCCCGTTgg < 1:618505/61‑1 (MQ=255) gTTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTAAATACAGTCTGCCCGTTgg < 1:352031/61‑1 (MQ=255) gTTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTAAATACAGTCTGCCCGTTgg < 1:905081/61‑1 (MQ=255) gTTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTAAATACAGTCTGCCCGTTgg < 1:1240615/61‑1 (MQ=255) tttCTGCCCACGAGACACGCAACGTGGTGCGGCTTTCGCTAAATACAGTCTGCCCGTTgg < 1:1271349/60‑1 (MQ=255) tCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTAAATACAGTCTGCCCGTTGGCGGTAATcac > 1:2413421/1‑68 (MQ=255) tCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTAAATACAGTCTGCCCGTTGGCGGTAATcac > 1:2216990/1‑68 (MQ=255) tCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTAAATACAGTCTGCCCGTTGGCGGTAATcac > 1:2255742/1‑68 (MQ=255) tCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTAAATACAGTCTGCCCGTTGGCGGTAATcac > 1:1975857/1‑68 (MQ=255) tCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTAAATACAGTCTGCCCGTTGGCGGTAATcac > 1:458087/1‑68 (MQ=255) tCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTAAATACAGTCTGCCCGTTGGCGGTAATcac > 1:2664189/1‑68 (MQ=255) tCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTAAATACAGTCTGCCCGTTGGCGGTAATcac > 1:3392123/1‑68 (MQ=255) tCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTAAATACAGTCTGCCCGTTGGCGGTAATcac > 1:1434697/1‑68 (MQ=255) tCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTAAATACAGTCTGCCCGTTGGCGGTAATcac > 1:940311/1‑68 (MQ=255) tCTGCCCACCAGACAAGCAACGTGGTGAGGCTTTCGCTAAATACAGTCTGCCCGTTGGCGGTAATcac > 1:2909166/1‑68 (MQ=255) cTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTAAATACAGTCTGCCCGTTgg < 1:791996/57‑1 (MQ=255) cAACGTGGTGCGGCTTTCGCTGAATACAGTCTGGCCGTTGGCGGTAATCACAAAACGGTCACCGGAAAc > 1:3391653/1‑69 (MQ=255) cAACGTGGTGCGGCTTTCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACAAAACGGt > 1:1321517/1‑59 (MQ=255) cAACGTGGTGCGGCTTTCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACAAAACGGTCACCGGaaa > 1:620044/1‑68 (MQ=255) cAACGTGGTGCGGCTTTCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACAAAACGGTCACCGGAAAc > 1:1954678/1‑69 (MQ=255) cAACGTGGTGCGGCTTTCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACAAAACGGTCACCGGAAAc > 1:2420927/1‑69 (MQ=255) cAACGTGGTGCGGCTTTCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACAAAACGGTCACCGGAAAc > 1:2230933/1‑69 (MQ=255) cAACGTGGTGCGGCTTTCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACAAAACGGTCACCGGAAAc > 1:1708444/1‑69 (MQ=255) ggtgCGGCTTTCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACAAAACGGTCACCGGAAACCGCCTg < 1:2590610/69‑1 (MQ=255) gCGGCTTTCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACAAAACGGTCACCGGAAACCGCCTGccc > 1:1735668/1‑69 (MQ=255) gCGGCTTTCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACAAAACGGTCACCGGAAACCGCCTGccc > 1:577046/1‑69 (MQ=255) gCGGCTTTCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACAAAACGGTCACCGGAAACCGCCTGccc > 1:1284111/1‑69 (MQ=255) gCGGCTTTCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACAAAACGGTCACCGGAAACCGCCTGccc > 1:1312969/1‑69 (MQ=255) gCGGCTTTCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACAAAACGGTCACCGGAAACCGCCTGccc > 1:1707812/1‑69 (MQ=255) gCGGCTTTCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACAAAACGGTCACCGGAAACCGCCTGccc > 1:2763208/1‑69 (MQ=255) gCGGCTTTCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACAAAACGGTCACCGGAAACCGCCTGccc > 1:264213/1‑69 (MQ=255) gCGGCTTTCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACAAAACGGTCACCGGAAACCGCCTGccc > 1:2614221/1‑69 (MQ=255) gCTTTCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACAAAACGGTCACCGGAAACCGCCTGCCCTa > 1:1415498/1‑68 (MQ=255) gCTTTCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACAAAACGGTCACCGGAAACCGCCTGCCCTa > 1:2446596/1‑68 (MQ=255) gCTTTCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACAAAACGGTCACCGGAAACCGCCTGCCCTa > 1:1825012/1‑68 (MQ=255) gCTTTCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACAAAACGGTCACCGGAAACCGCCTGCCCTa > 1:1836752/1‑68 (MQ=255) gCTTTCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACAAAACGGTCACCGGAAACCGCCTGCCCTa > 1:1217625/1‑68 (MQ=255) tttCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACAAAACGGTCACCGGAAACCGCCTGCCCTACAt < 1:812005/69‑1 (MQ=255) tttCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACAAAACGGTCACCGGAAACCGCCTGCCCTACAt < 1:1118461/69‑1 (MQ=255) tttCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACAAAACGGTCACCGGAAACCGCCTGCCCTACAt < 1:583151/69‑1 (MQ=255) tttCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACAAAACGGTCACCGGAAACCGCCTGCCCTACAt < 1:3168886/69‑1 (MQ=255) tttCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACAAAACGGTCACCGGAAACCGCCTGCCCTACAt < 1:284872/69‑1 (MQ=255) tttCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACAAAACGGTCACCGGAAACCGCCTGCCCTACAt < 1:2337114/69‑1 (MQ=255) tttCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACAAAACGGTCACCGGAAACCGCCTGCCCTACAt < 1:2084180/69‑1 (MQ=255) tttCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACAAAACGGTCACCGGAAACCGCCTGCCCTACAt < 1:1974691/69‑1 (MQ=255) tttCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACAAAACGGTCACCGGAAACCGCCTGCCCTACAt < 1:1784307/69‑1 (MQ=255) tttCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACAAAACGGTCACCGGAAACCGCCTGCCCTACAt < 1:152130/69‑1 (MQ=255) tttCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACAAAACGGTCACCGGAAACCGCCTGCCCTACAt < 1:1512388/69‑1 (MQ=255) tttCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACAAAACGGTCACCGGAAACCGCCTCCCCTACAt < 1:954872/69‑1 (MQ=255) ttCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACAAAACGGTCACCGGAAACCGCCTGCCCTACATa > 1:2389325/1‑69 (MQ=255) ttCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACAAAACGGTCACCGGAAACCGCCTGCCCTACATa > 1:1372787/1‑69 (MQ=255) | GCAGGCGCTGCATCTGCCAGGTAGTTTCTGCCCACCAGACACGCAACGTGGTGCGGCTTTCGCTGAATACAGTCTGCCCGTTGGCGGTAATCACAAAACGGTCACCGGAAACCGCCTGCCCTACATA > minE/1725244‑1725370 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |