| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,727,498 | A→G | 30.8% | T244T (ACT→ACC) | purL ← | phosphoribosylformyl‑glycineamide synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,727,498 | 0 | A | G | 30.8% | 83.4 / 45.5 | 94 | T244T (ACT→ACC) | purL | phosphoribosylformyl‑glycineamide synthetase |
| Reads supporting (aligned to +/- strand): ref base A (40/25); new base G (11/18); total (51/43) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.43e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01 | |||||||||||
CCTTCCATTACGGCGGCGTTATCTTTATAAGCAGAGAGAACGTGATCTGGCGTGGTTTCGAAAGTATTTTTGATCATCTTGAACAGCGATTTCGGCTGCTGTTCACCATCGATAACCCAGTCGGC > minE/1727436‑1727560 | ccTTCCATTACGGCGGCGTTATCTTTATAAGCAGAGAGAACGTGATCTGGCGTGGTTTCGAAAGTAttt > 1:293882/1‑69 (MQ=255) tCCATTACGGCGGCGTTATCTTTATAAGCAGAGAGAACGTGATCTGGCGTGGTTTCGAAAGTATTTTTg < 1:648307/69‑1 (MQ=255) tCCATTACGGCGGCGTTATCTTTATAAGCAGAGAGAACGTGATCTGGCGTGGTTTCGAAAGTATTTTTg < 1:3202163/69‑1 (MQ=255) tCCATTACGGCGGCGTTATCTTTATAAGCAGAGAGAACGTGATCTGGCGTGGTTTCGAAAGTATTTTTg < 1:2426482/69‑1 (MQ=255) ttACGGCGGCGTTATCTTTATAAGCAGAGAGAACGTGATCTGGCGTGGTTTCGAAAGTATTTTTGatca < 1:1861824/69‑1 (MQ=255) ttACGGCGGCGTTATCTTTATAAGCAGAGAGAACGTGATCTGGCGTGGTTTCGAAAGTATTTTTGatca < 1:1816331/69‑1 (MQ=255) ttACGGCGGCGTTATCTTTATAAGCAGAGAGAACGTGATCTGGCGTGGTTTCGAAAGTATTTTTGatca < 1:1988204/69‑1 (MQ=255) ttACGGCGGCGTTATCTTTATAAGCAGAGAGAACGTGATCTGGCGTGGTTTCGAAAGTATTTTTGatca < 1:2089111/69‑1 (MQ=255) ttACGGCGGCGTTATCTTTATAAGCAGAGAGAACGTGATCTGGCGTGGTTTCGAAAGTATTTTTGatca < 1:2094103/69‑1 (MQ=255) ttACGGCGGCGTTATCTTTATAAGCAGAGAGAACGTGATCTGGCGTGGTTTCGAAAGTATTTTTGatca < 1:791225/69‑1 (MQ=255) ttACGGCGGCGTTATCTTTATAAGCAGAGAGAACGTGATCTGGCGTGGTTTCGAAAGTATTTTTGatca < 1:2639849/69‑1 (MQ=255) ttACGGCGGCGTTATCTTTAAAAGCAGAGAGAACGTGATCTGGCGTGGTTTCGAAAGTATTTTTGatca < 1:324657/69‑1 (MQ=255) aCGGCGGCGTTATCTTTATAAGCAGAGAGAACGTGATCTGGCGTGGTTTCGAAAGTATTTTTGatcatc > 1:847802/1‑69 (MQ=255) aCGGCGGCGTTATCTTTATAAGCAGAGAGAACGTGATCTGGCGTGGTTTCGAAAGTATTTTTGatcatc > 1:1994026/1‑69 (MQ=255) aCGGCGGCGTTATCTTTATAAGCAGAGAGAACGTGATCTGGCGTGGTTTCGAAAGTATTTTTGatcatc > 1:1966230/1‑69 (MQ=255) aCGGCGGCGTTATCTTTATAAGCAGAGAGAACGTGATCTGGCGTGGTTTCGAAAGTATTTTTGatcatc > 1:593140/1‑69 (MQ=255) aCGGCGGCGTTATCTTTATAAGCAGAGAGAACGTGATCTGGCGTGGTTTCGAAAGTATTTTTGatcatc > 1:2103482/1‑69 (MQ=255) aCGGCGGCGTTATCTTTATAAGCAGAGAGAACGTGATCTGGCGTGGTTTCGAAAGTATTTTTGatcatc > 1:1730375/1‑69 (MQ=255) aCGGCGGCGTTATCTTTATAAGCAGAGAGAACGTGATCTGGCGTGGTTTCGAAAGTATTTTTGatcatc > 1:866837/1‑69 (MQ=255) aCGGCGGCGTTATCTTTATAAGCAGAGAGAACGTGATCTGGCGTGGTTTCGAAAGTATTTTTGatcat > 1:2877035/1‑68 (MQ=255) aCGGCGGCGTTATCTTTATAAGCAGAGAGAACGTGATCTGGCGTGGTTTCGAAAGTATTTTTGatcat > 1:3104646/1‑68 (MQ=255) aCGGCGGCGTTATCTTTATAAGCAGAGAGAACGTGATCTGGCGTGGTTTCGAAAGTATTTTTGatcat > 1:799949/1‑68 (MQ=255) aCGGCGGCGTTATCTTTATAAGCAGAGAGAACGTGATCTGGCGTGGTTTCGAAAGTATTTTTGatc > 1:2464182/1‑66 (MQ=255) aCGGCGGCGTTATCTTTATAAGCAGAGAGAACGTGATCTGGCGTGGTTTCGAAAGTATTTTTGatc > 1:265102/1‑66 (MQ=255) aCGGCGGCGTTATCTTTATAAGCAGAGAGAACGTGATCTGGCGTGGTTTCGAAAGTATTTTTGatc > 1:2921309/1‑66 (MQ=255) aCGGCGGCGTTATCTTTATAAGCAGAGAGAACGTGATCTGGCGTGGTTTCGAAAGTATTTTTGatc > 1:2081112/1‑66 (MQ=255) aCGGCGGCGTTATCTTTATAAGCAGAGAGAACGTGATCTGGCGTGGTTTCGAAAGTATTTTTGataat > 1:3041343/1‑68 (MQ=255) aCGGCGGCGTTATCTTTATAAGCAGAGAGAACGTGATCTGGCGTGGTTTCGAAAGTATTTTTGat > 1:2184566/1‑65 (MQ=255) aCGGCGGCGTTATCTTTATAAGCAGAGAGAACGTGATCTGGCGTGGTTTCGAAAGTATTTTTGat > 1:1357066/1‑65 (MQ=255) aCGGCGGCGTTATCTTTATAAGCAGAGAGAACGTGATCTGGCGTGGTTTCGAAAGTAATTTTGata > 1:2607980/1‑65 (MQ=255) gcggcgTTATCTTTATAAGCAGAGAGAACGTGATCTGGCGTGGTTTCGAAAGTATTTTTGATCATCTTg < 1:2573931/69‑1 (MQ=255) gcggcgTTATCTTTATAAGCAGAGAGAACGTGATCTGGCGTGGTTTCGAAAGTATTTTTGATCATCTTg < 1:739945/69‑1 (MQ=255) gcggcgTTATCTTTATAAGCAGAGAGAACGTGATCTGGCGTGGTTTCGAAAGTATTTTTGATCATCTTg < 1:425142/69‑1 (MQ=255) gcggcgTTATCTTTATAAGCAGAGAGAACGTGATCTGGCGTGGTTTCGAAAGTATTTTTGATCATCTTg < 1:909033/69‑1 (MQ=255) gcggcgTTATCTTTATAAGCAGAGAGAACGTGATCTGGCGTGGTTTCGAAAGTATTTTTGATCATCTTg < 1:1315488/69‑1 (MQ=255) gcggcgTTATCTTTATAAGCAGAGAGAACGTGATCTGGCGTGGTTTCGAAAGTATTTTTGATCATCTTg < 1:665539/69‑1 (MQ=255) gcggcgTTATCTTTATAAGCAGAGAGAACGTGATCTGGCGTGGTTTCGAAAGTATTTTTGATCATCTTg < 1:3157482/69‑1 (MQ=255) gcggcgTTATCTTTATAAGCAGAGAGAACGTGATCTGGCGTGGTTTCGAAAGTATTTTTGATCATCTTg < 1:1995283/69‑1 (MQ=255) gcggcgTTATCTTTATAAGCAGAGAGAACGTGATCTGGCGTGGTTTCGAAAGTATTTTTGATCATCTTg > 1:1848697/1‑69 (MQ=255) cgTTATCTTTATCAGCAGAGAGAACGTGATCTGGCGTGGTTTCGAAAGTATTTTTGatca < 1:2357112/60‑1 (MQ=255) aTCTTTATAAGCAGAGAGAACGTGATCTGGCGTGGTTTCGAAAGTATTTTTGatc > 1:550915/1‑55 (MQ=255) aTCTTTATAAGCAGAGAGAACGTGATCTGGCGTGGTTTCGAAAGTATTTTTGATCATCTTGAACAGCg > 1:1459250/1‑68 (MQ=255) aTCTTTATAAGCAGAGAGAACGTGATCTGGCGTGGTTTCGAAAGTATTTTTGATCATCTTGAACAGCGa > 1:2886570/1‑69 (MQ=255) aTCTTTATAAGCAGAGAGAACGTGATCTGGCGTGGTTTCGAAAGTATTTTTGATCATCTTGAACAGCGa > 1:125265/1‑69 (MQ=255) aTCTTTATAAGCAGAGAGAACGTGATCTGGCGTGGTTTCGAAAGTATTTTTGATCATCTTGAACAGCGa > 1:2887157/1‑69 (MQ=255) aTCTTTATAAGCAGAGAGAACGTGATCTGGCGTGGTTTCGAAAGTATTTTTGATCATCTTGAACAGCGa > 1:446968/1‑69 (MQ=255) aTCTTTATAAGCAGAGAGAACGTGATCTGGCGTGGTTTCGAAAGTATTTTTGATCATCTTGAACAGCGa > 1:3279848/1‑69 (MQ=255) aTCTTTATAAGCAGAGAGAACGTGATCTGGCGTGGTTTCGAAAGTATTTTTGATCATCTTGAACAGCGa > 1:3311711/1‑69 (MQ=255) aTCTTTATAAGCAGAGAGAACGTGATCTGGCGTGGTTTCGAAAGTATTTTTGATCATCTTGAACAGCGa > 1:2295700/1‑69 (MQ=255) aTCTTTATAAGCAGAGAGAACGTGATCTGGCGTGGTTTCGAAAGTATTTTTGATCATCTTGAACAGCGa > 1:2034295/1‑69 (MQ=255) aTCTTTATAAGCAGAGAGAACGTGATCTGGCGTGGTTTCGAAAGTATTTTTGATCATCTTGAACAGCGa > 1:1637001/1‑69 (MQ=255) aTCTTTATAAGCAGAGAGAACGTGATCTGGCGTGGTTTCGAAAGTATTTTTGATCATCTTGAACAGCGa > 1:848091/1‑69 (MQ=255) cAGAGAGAACGTGATCTGGCGTGGTTTCGAAAGTATTTTTGATCATCTTGAACAGCGATTTCGgctgc < 1:2557372/68‑1 (MQ=255) aCGTGGTCTGGCGTGGTTTCGAAGGTGTTTTTGat > 1:90758/1‑35 (MQ=25) aCGTGGTCTGGCGTGGTTTCGAAGGTGTTTTTGATCATCTTGAACAGCGATTTCGGCTGCTGTTCAccg > 1:3142353/1‑68 (MQ=255) aCGTGGTCTGGCGTGGTTTCGAAGGTGTTTTTGATCATCTTGAACAGCGATTTCGGCTGCTGTTCAccg > 1:44021/1‑68 (MQ=255) aCGTGGTCTGGCGTGGTTTCGAAGGTGTTTTTGATCATCTTGAACAGCGATTTCGGCTGCTGTTCAccg > 1:1168218/1‑68 (MQ=255) aCGTGGTCTGGCGTGGTTTCGAAGGTGTTTTTGATCATCTTGAACAGCGATTTCGGCTGCTGTTCAccg > 1:2951969/1‑68 (MQ=255) aCGTGGTCTGGCGTGGTTTCGAAGGTGTTTTTGATCATCTTGAACAGCGATTTCGGCTGCTGTTCAccg > 1:2606877/1‑68 (MQ=255) aCGTGGTCTGGCGTGGTTTCGAAGGTGTTTTTGATCATCTTGAACAGCGATTTCGGCTGCTGTTCAccg > 1:774261/1‑68 (MQ=255) aCGTGGTCTGGCGTGGTTTCGAAGGTGTTTTTGATCATCTTGAACAGCGATTTCGGCTGCTGTTCAccg > 1:2174095/1‑68 (MQ=255) aCGTGGTCTGGCGTGGTTTCGAAGGTGTTTTTGATCATCTTGAACAGCGATTTCGGCTGCTGTTCAccg > 1:2070427/1‑68 (MQ=255) aCGTGGTCTGGCGTGGTTTCGAAGGTGTTTTTGATCATCTTGAACAGCGATTTCGGCTGCTGTTCAccg > 1:903088/1‑68 (MQ=255) aCGTGGTCTGGCGTGGTTTCGAAGGTGTTTTTGATCATCTTGAACAGCGATTTCGGCTGCTGTTCAccg > 1:1103342/1‑68 (MQ=255) aTCTGGCGTGGTTTCGAAAGTATTTTTGATCATCTTGAACAGCGATTTCGGCTGCTGTTCACCATCGAt < 1:556997/69‑1 (MQ=255) aTCTGGCGTGGTTTCGAAAGTATTTTTGATCATCTTGAACAGCGATTTCGGCTGCTGTTCACCATCGAt < 1:2741503/69‑1 (MQ=255) aTCTGGCGTGGTTTCGAAAGTATTTTTGATCATCTTGAACAGCGATTTCGGCTGCTGTTCACCATCGAt < 1:1630729/69‑1 (MQ=255) gTGGTTTCGAAGGTGTTTTTGATCATCTTGAACAGCGATTTCGGCTGCTGTTCACCGTCGATAAtcc < 1:3338136/67‑1 (MQ=255) gTGGTTTCGAAGGTGTTTTTGATCATCTTGAACAGCGATTTCGGCTGCTGTTCACCGTCGATAAtcc < 1:913536/67‑1 (MQ=255) gTGGTTTCGAAGGTGTTTTTGATCATCTTGAACAGCGATTTCGGCTGCTGTTCACCGTCGATAAtcc < 1:330577/67‑1 (MQ=255) gTGGTTTCGAAGGTGTTTTTGATCATCTTGAACAGCGATTTCGGCTGCTGTTCACCGTCGATAAtcc < 1:276468/67‑1 (MQ=255) gTGGTTTCGAAGGTGTTTTTGATCATCTTGAACAGCGATTTCGGCTGCTGTTCACCGTCGATAAtcc < 1:1288209/67‑1 (MQ=255) gTGGTTTCGAAGGTGTTTTTGATCATCTTGAACAGCGATTTCGGCTGCTGTTCACCGTCGATAAtcc < 1:1365691/67‑1 (MQ=255) gTGGTTTCGAAGGTGTTTTTGATCATCTTGAACAGCGATTTCGGCTGCTGTTCACCGTCGATAAtcc < 1:1514339/67‑1 (MQ=255) gTGGTTTCGAAGGTGTTTTTGATCATCTTGAACAGCGATTTCGGCTGCTGTTCACCGTCGATAAtcc < 1:1569854/67‑1 (MQ=255) gTGGTTTCGAAGGTGTTTTTGATCATCTTGAACAGCGATTTCGGCTGCTGTTCACCGTCGATAAtcc < 1:1785623/67‑1 (MQ=255) gTGGTTTCGAAGGTGTTTTTGATCATCTTGAACAGCGATTTCGGCTGCTGTTCACCGTCGATAAtcc < 1:2065498/67‑1 (MQ=255) gTGGTTTCGAAGGTGTTTTTGATCATCTTGAACAGCGATTTCGGCTGCTGTTCACCGTCGATAAtcc < 1:2490660/67‑1 (MQ=255) gTGGTTTCGAAGGTGTTTTTGATCATCTTGAACAGCGATTTCGGCTGCTGTTCACCGTCGATAAtcc < 1:2819495/67‑1 (MQ=255) gTGGTTTCGAAGGTGTTTTTGATCATCTTGAACAGCGATTTCGGCTGCTGTTCACCGTCGATAAtcc < 1:2832330/67‑1 (MQ=255) gTGGTTTCGAAGGTGTTTTTGATCATCTTGAACAGCGATTTCGGCTGCTGTTCACCGTCGATAAtcc < 1:2886599/67‑1 (MQ=255) gTGGTTTCGAAGGTGTTTTTGATCATCTTGAACAGCGATTTCGGCTGCTGTTCACCGTCGATAAtcc < 1:2936407/67‑1 (MQ=255) gTGGTTTCGAAGGTGTTTTTGATCATCTTGAACAGCGATTTCGGCTGCTGTTCACCGTCGATAAtcc < 1:3018224/67‑1 (MQ=255) gTGGTTTCGAAGGTGTTTTTGATCATCTTGAACAGCGATTTCGGCTGCTGTTCACCGTCGATAAtcc < 1:3122211/67‑1 (MQ=255) gTGGTTTCGAAGGTGTTTTTGATCATCTTGAACAGCGATTTCGGCTGCTGTTCACCGTCGATAAtcc < 1:3271764/67‑1 (MQ=255) ttCGAAAGTATTTTTGATCATCTTGAACAGCGATTTCGgctgc < 1:2561360/43‑1 (MQ=255) tCGAAAGTATTTTTGATCATCTTGAACAGCGATTTCGGCTGCTGTTCACCATCg > 1:567220/1‑54 (MQ=255) tCGAAAGTATTTTTGATCATCTTGAACAGCGATTTCGGCTGCTGTTCACCATCg > 1:3286868/1‑54 (MQ=255) tCGAAAGTATTTTTGATCATCTTGAACAGCGATTTCGGCTGCTGTTCACCATCg > 1:2504459/1‑54 (MQ=255) tCGAAAGTATTTTTGATCATCTTGAACAGCGATTTCGGCTGCTGTTCACCATCg > 1:2141451/1‑54 (MQ=255) tCGAAAGTATTTTTGATCATCTTGAACAGCGATTTCGGCTGCTGTTCACCATCg > 1:3341856/1‑54 (MQ=255) tCGAAAGTATTTTTGATCATCTTGAACAGCGATTTCGGCTGCTGTTCACCATCg > 1:1297068/1‑54 (MQ=255) tCGAAAGTATTTTTGATCATCTTGAACAGCGATTTCGGCTGCTGTTCACCATCGATAACCCAGTCGGc > 1:2903214/1‑68 (MQ=255) tCGAAAGTATTTTTGATCATCTTGAACAGCGATTTCGGCTGCTGTTCACCATCGATAACCCAGTCGGc > 1:3052325/1‑68 (MQ=255) | CCTTCCATTACGGCGGCGTTATCTTTATAAGCAGAGAGAACGTGATCTGGCGTGGTTTCGAAAGTATTTTTGATCATCTTGAACAGCGATTTCGGCTGCTGTTCACCATCGATAACCCAGTCGGC > minE/1727436‑1727560 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |