| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 131,549 | G→A | 39.1% | intergenic (‑115/‑159) | panD ← / → yadD | aspartate 1‑decarboxylase/predicted transposase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 131,549 | 0 | G | A | 39.1% | 48.4 / 65.6 | 97 | intergenic (‑115/‑159) | panD/yadD | aspartate 1‑decarboxylase/predicted transposase |
| Reads supporting (aligned to +/- strand): ref base G (43/16); new base A (10/28); total (53/44) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.85e-06 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.09e-01 | |||||||||||
CTCACGCAGTATTGCCCGATTTTTGTTTAGTGTCTACTCATCTGACGGCATTTGCGTCAGCAGTTTGCGTACCGCGCCGAAGCGTGCCATGTATCGCCTGTAACGAATTGATATTTCTCTG > minE/131494‑131614 | ctcACGCAGTATTGCCCGATTTTTGTTTAGTGTCTACTCATCTGACGGCATTTGCGTCAGCAGTTTGCg > 1:2397208/1‑69 (MQ=255)ctcACGCAGTATTGCCCGATTTTTGTTTAGTGTCTACTCATCTGACGGCATTTGCGTCAGCAGTTTGCg > 1:783610/1‑69 (MQ=255) aTTGCCCGATTTTTGTTTAGTGTCTACTCATCTGACGGCATTTGCGTCAGCAGTTTGCGTACCgcgc > 1:1037344/1‑67 (MQ=255) aTTGCCCGATTTTTGTTTAGTGTCTACTCATCTGACGGCATTTGCGTCAGCAGTTTGCGTACCgcgc > 1:370325/1‑67 (MQ=255) aTTGCCCGATTTTTGTTTAGTGTCTACTCATCTGACGGCATTTGCGTCAGCAGTTTGCGTACCgcgc > 1:2898884/1‑67 (MQ=255) ttGCCCGATTTTTGTTTAGTGTCTACTCATCTGACGGCATTTGCGTCAGCAGTTTGCGTACCgcgcag > 1:780126/1‑66 (MQ=255) ttGCCCGATTTTTGTTTAGTGTCTACTCATCTGACGGCATTTGCGTCAGCAGTTTGCGTACCGCGCCGa < 1:778377/69‑1 (MQ=255) ttGCCCGATTTTTGTTTAGTGTCTACTCATCTGACGGCATTTGCGTCAGCAGTTTGCGTACCGCGCCGa > 1:3035697/1‑69 (MQ=255) gCCCGATTTTTGTTTAGTGTCTACTCATCTGACGGCATTTGCGTcagc > 1:1074793/1‑48 (MQ=255) gCCCGATTTTTGTTTAGTGTCTACTCATCTGACGGCATTTGCGTcag > 1:1994895/1‑47 (MQ=255) gCCCGATTTTTGTTTAGTGTCTACTCATCTGACGGCATTTGCGTCAGCAGTTTGCGTACCGCGCCGAAg > 1:2759544/1‑69 (MQ=255) gCCCGATTTTTGTTTAGTGTCTACTCATCTGACGGCATTTGCGTCAGCAGTTTGCGTACCGCGCCGAAg > 1:2736903/1‑69 (MQ=255) gCCCGATTTTTGTTTAGTGTCTACTCATCTGACGGCATTTGCGTCAGCAGTTTGCGTACCGCGCCGAAg > 1:3359716/1‑69 (MQ=255) gCCCGATTTTTGTTTAGTGTCTACTCATCTGACGGCATTTGCGTCAGCAGTTTGCGTACCGCGCCGAAg > 1:33618/1‑69 (MQ=255) gCCCGATTTTTGTTTAGTGTCTACTCATCTGACGGCATTTGCGTCAGCAGTTTGCGTACCGCGCCGAAg > 1:3783/1‑69 (MQ=255) gCCCGATTTTTGTTTAGTGTCTACTCATCTGACGGCATTTGCGTCAGCAGTTTGCGTACCGCGCCGAAg > 1:45107/1‑69 (MQ=255) gCCCGATTTTTGTTTAGTGTCTACTCATCTGACGGCATTTGCGTCAGCAGTTTGCGTACCGCGCCGAAg > 1:178199/1‑69 (MQ=255) gCCCGATTTTTGTTTAGTGTCTACTCATCTGACGGCATTTGCGTCAGCAGTTTGCGTACCGCGCCGAAg > 1:1777627/1‑69 (MQ=255) gCCCGATTTTTGTTTAGTGTCTACTCATCTGACGGCATTTGCGTCAGCAGTTTGCGTACCGCGCCGAAg > 1:1775587/1‑69 (MQ=255) gCCCGATTTTTGTTTAGTGTCTACTCATCTGACGGCATTTGCGTCAGCAGTTTGCGTACCGCGCCGAAg > 1:1158453/1‑69 (MQ=255) gCCCGATTTTTGTTTAGTGTCTACTCATCTGACGGCATTTGCGTCAGCAGTTTGCGTACCGCGCCGAAg > 1:1323462/1‑69 (MQ=255) gCCCGATTTTTGTTTAGTGTCTACTCATCTGACGGCATTTGCGTCAGCAGTTTGCGTACCGCGCCGAAg > 1:1576563/1‑69 (MQ=255) gCCCGATTTTTGTTTAGTGTCTACTCATCTGACGGCATTTGCGTCAGCAGTTTGCGTACCGCGCCGAAg > 1:1429078/1‑69 (MQ=255) ttttGTTTAGTGTCTACTCATCTGACGGCATTTGCGTCAGCAGTTTGCGTACCGCGCCGAAGCGTGCCa > 1:2768083/1‑69 (MQ=255) ttttGTTTAGTGTCTACTCATCTGACGGCATTTGCGTCAGCAGTTTGCGTACCGCGCCGAAGCGTGCCa > 1:444675/1‑69 (MQ=255) ttttGTTTAGTGTCTACTCATCTGACGGCATTTGCGTCAGCAGTTTGCGTACCGCGCCGAAGCGTGCCa > 1:2047778/1‑69 (MQ=255) tttGTTTAGTGTCTACTCATCTGACGGCATTTGCGTCAGCAGTTTGCGTACCGCGCCGAAGCGTGCCa > 1:524273/1‑68 (MQ=255) tttGTTTAGTGTCTACTCATCTGACGGCATTTGCGTCAGCAGTTTGCGTACCGCGCCGAAGCGTGCCa > 1:2465573/1‑68 (MQ=255) tttGTTTAGTGTCTACTCATCTGACGGCATTTGCGTCAGCAGTTTGCGTACCGCGCCGAAGCGTGCCa > 1:3348021/1‑68 (MQ=255) gtCTACTTATCTGACGGCATTTGCAGCAGCAGTTTGCGTACCgtgc < 1:275548/46‑1 (MQ=255) gtCTACTCATCTGACGGCATTTTCAGCAGCAGTTTGCGTACCgtgc < 1:1656906/46‑1 (MQ=255) gtCTACTCATCTGACGGCATTTGCAGCAGCGGTTTGCGTACCgtgc < 1:866466/46‑1 (MQ=255) gtCTACTCATCTGACGGCATTTGCAGCAGCAGTTTGCGTACCgtgc < 1:703044/46‑1 (MQ=255) gtCTACTCATCTGACGGCATTTGCAGCAGCAGTTTGCGTACCgtgc < 1:3362438/46‑1 (MQ=255) gtCTACTCATCTGACGGCATTTGCAGCAGCAGTTTGCGTACCgtgc < 1:344682/46‑1 (MQ=255) gtCTACTCATCTGACGGCATTTGCAGCAGCAGTTTGCGTACCgtgc < 1:3194649/46‑1 (MQ=255) gtCTACTCATCTGACGGCATTTGCAGCAGCAGTTTGCGTACCgtgc < 1:3083744/46‑1 (MQ=255) gtCTACTCATCTGACGGCATTTGCAGCAGCAGTTTGCGTACCgtgc < 1:686271/46‑1 (MQ=255) gtCTACTCATCTGACGGCATTTGCAGCAGCAGTTTGCGTACCgtgc < 1:622698/46‑1 (MQ=255) gtCTACTCATCTGACGGCATTTGCAGCAGCAGTTTGCGTACCgtgc < 1:837954/46‑1 (MQ=255) gtCTACTCATCTGACGGCATTTGCAGCAGCAGTTTGCGTACCgtgc < 1:3026693/46‑1 (MQ=255) gtCTACTCATCTGACGGCATTTGCAGCAGCAGTTTGCGTACCgtgc < 1:2838400/46‑1 (MQ=255) gtCTACTCATCTGACGGCATTTGCAGCAGCAGTTTGCGTACCgtgc < 1:445520/46‑1 (MQ=255) gtCTACTCATCTGACGGCATTTGCAGCAGCAGTTTGCGTACCgtgc < 1:1064725/46‑1 (MQ=255) gtCTACTCATCTGACGGCATTTGCAGCAGCAGTTTGCGTACCgtgc < 1:2661399/46‑1 (MQ=255) gtCTACTCATCTGACGGCATTTGCAGCAGCAGTTTGCGTACCgtgc < 1:2084287/46‑1 (MQ=255) gtCTACTCATCTGACGGCATTTGCAGCAGCAGTTTGCGTACCgtgc < 1:201612/46‑1 (MQ=255) gtCTACTCATCTGACGGCATTTGCAGCAGCAGTTTGCGTACCgtgc < 1:116292/46‑1 (MQ=255) gtCTACTCATCTGACGGCATTTGCAGCAGCAGTTTGCGTACCgtgc < 1:1221646/46‑1 (MQ=255) gtCTACTCATCTGACGGCATTTGCAGCAGCAGTTTGCGTACCgtgc < 1:1364732/46‑1 (MQ=255) gtCTACTCATCTGACGGCATTTGCAGCAGCAGTTTGCGTACCgtgc < 1:2470409/46‑1 (MQ=255) gtCTACTCATCTGACGGCATTTGCAGCAGCAGTTTGCGTACCgtgc < 1:1640075/46‑1 (MQ=255) gtCTACTCATCTGACGGCATTTGCAGCAGCAGTTTGCGTACCgtgc < 1:250711/46‑1 (MQ=255) gtCTACTCATCTGACGGCATTTGCAGCAGCAGTTTGCGTACCgtgc < 1:1784661/46‑1 (MQ=255) gtCTACTCATCTGACGGCATTTGCAGCAGCAGTTTGCGTACCgtgc < 1:1959318/46‑1 (MQ=255) gtCTACTCATCTGACGGCATTTGCAGCAGCAGATTGCGTACCgtgc < 1:220163/46‑1 (MQ=255) tCTACTCATCTGACGGCATTTGCGTCAGCAGTTTGCGTACCGCGCCGAAGCGTg > 1:1833775/1‑54 (MQ=255) tCTACTCATCTGACGGCATTTGCGTCAGCAGTTTGCGTACCGCGCCGAAGCGTGCCATGTATCGCCTGt < 1:1859696/69‑1 (MQ=255) tCTACTCATCTGACGGCATTTGCGTCAGCAGTTTGCGTACCGCGCCGAAGCGTGCCATGTATCGCCTGt < 1:1670661/69‑1 (MQ=255) tCTACTCATCTGACGGCATTTGCGTCAGCAGTTTGCGTACCGCGCCGAAGCGTGCCATGTATCGCCTGt < 1:1568279/69‑1 (MQ=255) tCTACTCATCTGACGGCATTTGCGTCAGCAGTTTGCGTACCGCGCCGAAGCGTGCCATGTATCGCCTGt < 1:982743/69‑1 (MQ=255) tCTACTCATCTGACGGCATTTGCGTCAGCAGTTTGCGTACCGCGCCGAAGCGTGCCATGTATCGCCTGt < 1:990227/69‑1 (MQ=255) tCTACTCATCTGACGGCATTTGCGTCAGCAGTTTGCGTACCGCGCCGAAGCGTGCCATGTATCGCCTGt < 1:2713780/69‑1 (MQ=255) tCTACTCATCTGACGGCATTTGCGTCAGCAGTTTGCGTACCGCGCCGAAGCGTGCCATGTATCGCCTGt < 1:3241950/69‑1 (MQ=255) tCTACTCATCTGACGGCATTTGCGTCAGCAGTTTGCGTACCGCGCCGAAGCGTGCCATGTATCGCCTGt < 1:22596/69‑1 (MQ=255) tCTACTCATCTGACGGCATTTGCGTCAGCAGTTTGCGTACCGCGCCGAAGCGTGCCATGTATCGCCTGt < 1:296461/69‑1 (MQ=255) tCTACTCATCTGACGGCATTTGCGTCAGCAGTTTGCGTACCGCGCCGAAGCGTGCCATGTATCGCCTGt < 1:2984348/69‑1 (MQ=255) tCTACTCATCTGACGGCATTTGCAGCAGCAGTTTGCGTACCgtgc < 1:2024857/45‑1 (MQ=255) tACTCATCTGACGGCATTTGCGTCAGCAGTTTGCGTa > 1:1206401/1‑37 (MQ=255) tACTCATCTGACGGCATTTGCGTCAGCAGTTTGCGTACCGCGCCGAAGCGTGCCATGTATCGCCTGTa > 1:2508605/1‑68 (MQ=255) tACTCATCTGACGGCATTTGCGTCAGCAGTTTGCGTACCGCGCCGAAGCGTGCCATGTATCGCCTGTa > 1:864082/1‑68 (MQ=255) tACTCATCTGACGGCATTTGCGTCAGCAGTTTGCGTACCGCGCCGAAGCGTGCCATGTATCGCCTGTa > 1:1646097/1‑68 (MQ=255) tACTCATCTGACGGCATTTGCGTCAGCAGTTTGCGTACCGCGCCGAAGCGTGCCATGTATCGCCTGTa > 1:2906359/1‑68 (MQ=255) tACTCATCTGACGGCATTTGCGTCAGCAGTTTGCGTACCGCGCCGAAGCGTGCCATGTATCGCCTGTa > 1:1821515/1‑68 (MQ=255) tACTCATCTGACGGCATTTGCGTCAGCAGTTTGCGTACCGCGCCGAAGCGTGCCATGTATCGCCTGTa > 1:2341992/1‑68 (MQ=255) tACTCATCTGACGGCATTTGCGTCAGCAGTTTGCGTACCGCGCCGAAGCGTGCCATGTATCGCCTGTa > 1:29998/1‑68 (MQ=255) cATCTGACGGCATTTGCGTCAGCAGTTTGCGTACCGCGCCGAAGCGTGCCATGTATCGCCTGTAACg < 1:2878152/67‑1 (MQ=255) cTGACGGCATTTGCGTCAGCAGTTTGCGTACCGCGCCGAAGCGTGCCATGTATCGCCTGt < 1:749328/60‑1 (MQ=255) cGGCATTTGCAGCAGCAGTTTGCGTACCGTGCCGAAGCGTGCCATGTAACGCCTGTAACCAATTGaaa > 1:2989319/1‑66 (MQ=255) cGGCATTTGCAGCAGCAGTTTGCGTACCGTGCCGAAGCGTGCCATGTAACGCCTGTAACCAATTGaaa > 1:2590849/1‑66 (MQ=255) cGGCATTTGCAGCAGCAGTTTGCGTACCGTGCCGAAGCGTGCCATGTAACGCCTGTAACCAATTGaaa > 1:972473/1‑66 (MQ=255) cGGCATTTGCAGCAGCAGTTTGCGTACCGTGCCGAAGCGTGCCATGTAACGCCTGTAACCAATTGaaa > 1:841889/1‑66 (MQ=255) cGGCATTTGCAGCAGCAGTTTGCGTACCGTGCCGAAGCGTGCCATGTAACGCCTGTAACCAATTGaaa > 1:805763/1‑66 (MQ=255) cGGCATTTGCAGCAGCAGTTTGCGTACCGTGCCGAAGCGTGCCATGTAACGCCTGTAACCAATTGaaa > 1:2538318/1‑66 (MQ=255) cGGCATTTGCAGCAGCAGTTTGCGTACCGTGCCGAAGCGTGCCATGTAACGCCTGTAACCAATTGaaa > 1:760794/1‑66 (MQ=255) cGGCATTTGCAGCAGCAGTTTGCGTACCGTGCCGAAGCGTGCCATGTAACGCCTGTAACCAATTGaaa > 1:636171/1‑66 (MQ=255) cGGCATTTGCAGCAGCAGTTTGCGTACCGTGCCGAAGCGTGCCATGTAACGCCTGTAACCAATTGaaa > 1:1865756/1‑66 (MQ=255) cGGCATTTGCAGCAGCAGTTTGCGTACCGTGCCGAAGCGTGCCATGTAACGCCTGTAACCAATTGaaa > 1:377912/1‑66 (MQ=255) ggaaTTTGCGTCAGCAGTTTGCGTACCGCGCCGAAGCGTGCCATGTATCGCCTGTAACGAATTGATAtt > 1:3299804/4‑69 (MQ=255) ggCATTTGCGTCAGCAGTTTGCGTACCGCGCCGAAGCGTGCCATGTATCGCCTGTAACGAATTGATAtt > 1:140838/1‑69 (MQ=255) ggCATTTGCGTCAGCAGTTTGCGTACCGCGCCGAAGCGTGCCATGTATCGCCTGTAACGAATTGATAtt > 1:733408/1‑69 (MQ=255) ggCATTTGCGTCAGCAGTTTGCGTACCGCGCCGAAGCGTGCCATGTATCGCCTGTAACGAATTGATAtt > 1:2546379/1‑69 (MQ=255) ggCATTTGCGTCAGCAGTTTGCGTACCGCGCCGAAGCGTGCCATGTATCGCCTGTAACGAATTGATAtt > 1:2574907/1‑69 (MQ=255) ggCATTTGCGTCAGCAGTTTGCGTACCGCGCCGAAGCGTGCCATGTATCGCCTGTAACGAATTGATAtt > 1:523636/1‑69 (MQ=255) tGCGTCAGCAGTTTGCGTACCGCGCCGAAGCGTGCCATGTATCGCCTGTAACGAATTGATATTTCTCTg < 1:854984/69‑1 (MQ=255) tGCGTCAGCAGTTTGCGTACCGCGCCGAAGCGTGCCATGTATCGCCTGTAACGAATTGATATTTCTCTg < 1:613334/69‑1 (MQ=255) tGCGTCAGCAGTTTGCGTACCGCGCCGAAGCGTGCCATGTATCGCCTGTAACGAATTGATATTTCTCTg < 1:2549188/69‑1 (MQ=255) | CTCACGCAGTATTGCCCGATTTTTGTTTAGTGTCTACTCATCTGACGGCATTTGCGTCAGCAGTTTGCGTACCGCGCCGAAGCGTGCCATGTATCGCCTGTAACGAATTGATATTTCTCTG > minE/131494‑131614 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |