| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,728,993 | A→C | 45.5% | E123D (GAA→GAC) | yfhD → | predicted transglycosylase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,728,993 | 0 | A | C | 45.5% | 3.8 / 115.0 | 99 | E123D (GAA→GAC) | yfhD | predicted transglycosylase |
| Reads supporting (aligned to +/- strand): ref base A (26/28); new base C (29/16); total (55/44) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.55e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.35e-01 | |||||||||||
Only 100 of 101 total aligned reads displayed.
GACGGCGGAGCAACTCACCGTTGCACCGGGTCATGTGGTGGTTAACGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAATGGAA > minE/1728927‑1729056 | gACGGCGGAGCAACTCACCGTTGCACCGGGTCATGTGGTGGTTAACGATCTCCAGACCCTGAAAGAAAc < 1:1041104/69‑1 (MQ=255)gACGGCGGAGCAACTCACCGTTGCACCGGGTCATGTGGTGGTTAACGATCTCCAGACCCTGAAAGAAAc < 1:981578/69‑1 (MQ=255)gACGGCGGAGCAACTCACCGTTGCACCGGGTCATGTGGTGGTTAACGATCTCCAGACCCTGAAAGAAAc < 1:2409088/69‑1 (MQ=255)gACGGCGGAGCAACTCACCGTTGCACCGGGTCATGTGGTGGTTAACGATCTCCAGACCCTGAAAGAAAc < 1:2392170/69‑1 (MQ=255) ggcggAGCAACTCACCGTTGCACCGGGTCATGTGGTGGTTAACGATCTCCAGACCCTGAAAGAAACaa < 1:1244189/68‑1 (MQ=255) ggcggAGCAACTCACCGTTGCACCGGGTCATGTGGTGGTTAACGATCTCCAGACCCTGAAAGAAACaa < 1:3245531/68‑1 (MQ=255) ggcggAGCAACTCACCGTTGCACCGGGTCATGTGGTGGTTAACGATCTCCAGACCCTGAAAGAAACaa < 1:3431322/68‑1 (MQ=255) ggcggAGCAACTCACCGTTGCACCGGGTCATGTGGTGGTTAACGATCTCCAGACCCTGAAAGAAACaa < 1:3419647/68‑1 (MQ=255) ggcggAGCAACTCACCGTTGCACCGGGTCATGTGGTGGTTAACGATCTCCAGACCCTGAAAGAAACaa < 1:1573586/68‑1 (MQ=255) cggAGCAACTCACCGTTGCACCGGGTCACGTGGTGGTTAACGATCTCCAGACCCTGAAAGACACaaa > 1:1446914/1‑67 (MQ=255) cggAGCAACTCACCGTTGCACCGGGTCACGTGGTGGTTAACGATCTCCAGACCCTGAAAGACACAAAAt > 1:1948824/1‑69 (MQ=255) cggAGCAACTCACCGTTGCACCGGGTCACGTGGTGGTTAACGATCTCCAGACCCTGAAAGACACAAAAt > 1:1208336/1‑69 (MQ=255) cggAGCAACTCACCGTTGCACCGGGTCACGTGGTGGTTAACGATCTCCAGACCCTGAAAGACACAAAAt > 1:2114776/1‑69 (MQ=255) cggAGCAACTCACCGTTGCACCGGGTCACGTGGTGGTTAACGATCTCCAGACCCTGAAAGACACAAAAt > 1:232981/1‑69 (MQ=255) cggAGCAACTCACCGTTGCACCGGGTCACGTGGTGGTTAACGATCTCCAGACCCTGAAAGACACAAAAt > 1:1043451/1‑69 (MQ=255) cggAGCAACTCACCGTTGCACCGGGTCACGTGGTGGTTAACGATCTCCAGACCCTGAAAGACACAAAAt > 1:2630881/1‑69 (MQ=255) cggAGCAACTCACCGTTGCACCGGGTCACGTGGTGGTTAACGATCTCCAGACCCTGAAAGACACAAAAt > 1:264996/1‑69 (MQ=255) cggAGCAACTCACCGTTGCACCGGGTCACGTGGTGGTTAACGATCTCCAGACCCTGAAAGACACAAAAt > 1:2889515/1‑69 (MQ=255) cggAGCAACTCACCGTTGCACCGGGTCACGTGGTGGTTAACGATCTCCAGACCCTGAAAGACACAAAAt > 1:3199746/1‑69 (MQ=255) cggAGCAACTCACCGTTGCACCGGGTCACGTGGTGGTTAACGATCTCCAGACCCTGAAAGACACAAAAt > 1:334345/1‑69 (MQ=255) cggAGCAACTCACCGTTGCACCGGGTCACGTGGTGGTTAACGATCTCCAGACCCTGAAAGACACAAAAt > 1:3434446/1‑69 (MQ=255) cggAGCAACTCACCGTTGCACCGGGTCACGTGGTGGTTAACGATCTCCAGACCCTGAAAGACACAAAAt > 1:364676/1‑69 (MQ=255) cggAGCAACTCACCGTTGCACCGGGTCACGTGGTGGTTAACGATCTCCAGACCCTGAAAGACACAAAAt > 1:42572/1‑69 (MQ=255) cggAGCAACTCACCGTTGCACCGGGTCACGTGGTGGTTAACGATCTCCAGACCCTGAAAGACACAAAAt > 1:492085/1‑69 (MQ=255) cggAGCAACTCACCGTTGCACCGGGTCACGTGGTGGTTAACGATCTCCAGACCCTGAAAGACACAAAAt > 1:666564/1‑69 (MQ=255) cggAGCAACTCACCGTTGCACCGGGTCACGTGGTGGTTAACGATCTCCAGACCCTGAAAGACACAAAAt > 1:669087/1‑69 (MQ=255) cggAGCAACTCACCGTTGCACCGGGTCACGTGGTGGTTAACGATCTCCAGACCCTGAAAGACACAAAAt > 1:81847/1‑69 (MQ=255) cggAGCAACTCACCGTTGCACCGGGTCACGTGGTGGTTAACGATCTCCAGACCCTGAAAGACACAAAAt > 1:867099/1‑69 (MQ=255) cggAGCAACTCACCGTTGCACCGGGTCACGTGGTGGTTAACGATCTCCAGACCCTGAAAGACACAAAAt > 1:871179/1‑69 (MQ=255) cggAGCAACTCACCGTTGCACCGGGTCACGTGGTGGTTAACGATCTCCAGACCCTGAAAGACACAAAAt > 1:1301866/1‑69 (MQ=255) cggAGCAACTCACCGTTGCACCGGGTCACGTGGTGGTTAACGATCTCCAGACCCTGAAAGACACAAAAt > 1:1596186/1‑69 (MQ=255) cggAGCAACTCACCGTTGCACCGGGTCACGTGGTGGTTAACGATCTCCAGACCCTGAAAGACACAAAAt > 1:18404/1‑69 (MQ=255) cggAGCAACTCACCGTTGCACCGGGTCACGTGGTGGTTAACGATCTCCAGACCCTGAAAGACA‑AAAAtt > 1:1963717/1‑69 (MQ=255) gCAACTCACCGTTGCACCTGGTCACGTGGTGGTTAACGATCTCCAGACCCTGAAAGACACaaaa < 1:3094962/64‑1 (MQ=255) gCAACTCACCGTTGCACCTGGTCACGTGGTGGTTAACGATCTCCAGACCCTGAAAGACACaaaa < 1:1326560/64‑1 (MQ=255) gCAACTCACCGTTGCACCGGGTCACGTGGTGGTTAACGATCTCCAGACCCTGAAAGACACaaaa < 1:900496/64‑1 (MQ=255) gCAACTCACCGTTGCACCGGGTCACGTGGTGGTTAACGATCTCCAGACCCTGAAAGACACaaaa < 1:1387091/64‑1 (MQ=255) gCAACTCACCGTTGCACCGGGTCACGTGGTGGTTAACGATCTCCAGACCCTGAAAGACACaaaa < 1:374186/64‑1 (MQ=255) gCAACTCACCGTTGCACCGGGTCACGTGGTGGTTAACGATCTCCAGACCCTGAAAGACACaaaa < 1:337295/64‑1 (MQ=255) gCAACTCACCGTTGCACCGGGTCACGTGGTGGTTAACGATCTCCAGACCCTGAAAGACACaaaa < 1:1917948/64‑1 (MQ=255) gCAACTCACCGTTGCACCGGGTCACGTGGTGGTTAACGATCTCCAGACCCTGAAAGACACaaaa < 1:1086872/64‑1 (MQ=255) gCAACTCACCGTTGCACCGGGTCACGTGGTGGTTAACGATCTCCAGACCCTGAAAGACACaaaa < 1:2394630/64‑1 (MQ=255) gCAACTCACCGTTGCACCGGGTCACGTGGTGGTTAACGATCTCCAGACCCTGAAAGACACaaaa < 1:1823246/64‑1 (MQ=255) gCAACTCACCGTTGCACCGGGTCACGTGGTGGTTAACGATCTCCAGACCCTGAAAGACACaaaa < 1:1356233/64‑1 (MQ=255) gCAACTCACCGTTGCACCGGGTCACGTGGTGGTTAACGATCTCCAGACCCTGAAAGACACaaaa < 1:1253586/64‑1 (MQ=255) gCAACTCACCGTTGCACCGGGTCACGTGGTGGTTAACGATCTCCAGACCCTGAAAGACACaaaa < 1:601818/64‑1 (MQ=255) gCAACTCACCGTTGCACCGGGTCACGTGGTGGTTAACGATCTCCAGACCCTGAAAGACACaaaa < 1:2496212/64‑1 (MQ=255) gCAACTCACCGTTGCACAGGGTCACGTGGTGGTTAACGATCTCCAGACCCTGAAAGACACaaaa < 1:569311/64‑1 (MQ=255) cAACTCACCGTTGCACCGGGTCACGTGGTGGTTAACGATCTCCAGACCCTGAAAGACACaaaa < 1:3257377/63‑1 (MQ=255) ttGCACCGGGTCACGTGGTGGTTAACGATCTCCAGACCCTGAAAGACACAAAATTCCCGGAATTAAGc > 1:2407148/1‑68 (MQ=255) ttGCACCGGGTCACGTGGTGGTTAACGATCTCCAGACCCTGAAAGACACAAAATTCCCGGAATTAAGc > 1:785514/1‑68 (MQ=255) ttGCACCGGGTCACGTGGTGGTTAACGATCTCCAGACCCTGAAAGACACAAAATTCCCGGAATTAAGc > 1:301046/1‑68 (MQ=255) ttGCACCGGGTCACGTGGTGGTTAACGATCTCCAGACCCTGAAAGACACAAAATTCCCGGAATTAAGc > 1:2236635/1‑68 (MQ=255) ttGCACCGGGTCACGTGGTGGTTAACGATCTCCAGACCCTGAAAGACACAAAATTCCCGGAATTAAGc > 1:133171/1‑68 (MQ=255) gCACCGGGTCATGTGGTGGTTAACGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTgg < 1:884501/69‑1 (MQ=255) aTGTGGTGGTTAACGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAgac > 1:1516671/1‑68 (MQ=255) aTGTGGTGGTTAACGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAgac > 1:2158353/1‑68 (MQ=255) gtTAACGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACaaaaaa > 1:1577203/1‑69 (MQ=255) gtTAACGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACaaaaaa > 1:2727530/1‑69 (MQ=255) gtTAACGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACaaaaaa > 1:3054452/1‑69 (MQ=255) gtTAACGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACaaaaaa > 1:1805051/1‑69 (MQ=255) gtTAACGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACaaaaaa > 1:827329/1‑69 (MQ=255) gtTAACGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACaaaaa > 1:2896833/1‑68 (MQ=255) gtTAACGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACaaaaa > 1:2858661/1‑68 (MQ=255) gtTAACGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACaa > 1:1251548/1‑65 (MQ=255) gtTAACGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACa > 1:837390/1‑64 (MQ=255) tAACGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAgg > 1:1012179/1‑69 (MQ=255) tAACGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAgg > 1:683558/1‑69 (MQ=255) tAACGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAgg > 1:2062370/1‑69 (MQ=255) tAACGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAgg > 1:2231065/1‑69 (MQ=255) tAACGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAgg > 1:534030/1‑69 (MQ=255) tAACGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAgg > 1:2424718/1‑69 (MQ=255) tAACGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAgg > 1:3243801/1‑69 (MQ=255) tAACGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAg > 1:2428646/1‑68 (MQ=255) tAACGATCTCCAGACCCTGAAAGAAACAAAATTCACGGAATTAAGCTGGAAGGTAGACGACAAAAAAgg > 1:2260723/1‑69 (MQ=255) tAACGATCTACAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAgg > 1:190973/1‑69 (MQ=255) aaCGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTTGAAGGTAGACGACaa < 1:1851262/62‑1 (MQ=255) aaCGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACaa < 1:85059/62‑1 (MQ=255) aaCGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACaa < 1:3070512/62‑1 (MQ=255) aaCGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACaa < 1:229287/62‑1 (MQ=255) aaCGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACaa < 1:2534335/62‑1 (MQ=255) aaCGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACaa < 1:1447335/62‑1 (MQ=255) aaCGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACaa < 1:2567546/62‑1 (MQ=255) aaCGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACaa < 1:2117119/62‑1 (MQ=255) aaCGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACaa < 1:2916472/62‑1 (MQ=255) aaCGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACaa < 1:2852374/62‑1 (MQ=255) aaCGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACaa < 1:462457/62‑1 (MQ=255) aaCGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGc < 1:2901974/69‑1 (MQ=255) aaCGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGc < 1:183419/69‑1 (MQ=255) aaCGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGc < 1:712963/69‑1 (MQ=255) aaCGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGc < 1:32362/69‑1 (MQ=255) aCGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGc < 1:13949/68‑1 (MQ=255) ctcCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTATACGACAAAAAAGGCTCTGc > 1:1897091/1‑68 (MQ=255) ctcCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGc > 1:749388/1‑68 (MQ=255) ctcCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGc > 1:1144494/1‑68 (MQ=255) ctcCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGc > 1:194250/1‑68 (MQ=255) ctcCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGc > 1:2959488/1‑68 (MQ=255) gACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCgg < 1:1353283/65‑1 (MQ=255) agaaaCAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAATGGaa < 1:2458641/67‑1 (MQ=255) agaaaCAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAATGGaa < 1:609454/67‑1 (MQ=255) | GACGGCGGAGCAACTCACCGTTGCACCGGGTCATGTGGTGGTTAACGATCTCCAGACCCTGAAAGAAACAAAATTCCCGGAATTAAGCTGGAAGGTAGACGACAAAAAAGGCTCTGCGGAATTAATGGAA > minE/1728927‑1729056 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |