| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,730,046 | C→T | 36.0% | T177T (ACG→ACA) | tadA ← | tRNA‑specific adenosine deaminase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,730,046 | 0 | C | T | 36.0% | 44.7 / 38.6 | 70 | T177T (ACG→ACA) | tadA | tRNA‑specific adenosine deaminase |
| Reads supporting (aligned to +/- strand): ref base C (11/33); new base T (16/9); total (28/42) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.08e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01 | |||||||||||
TGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACAAAATTAATCCGTCGAGGATTGCGCTTTTTTCTGCGCTTTAATTTCCTGGCGGCGCATGCGAAAGAAGT > minE/1729985‑1730104 | tGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACAAAATTAATCCGTCGAg < 1:900039/68‑1 (MQ=255)tGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACAAAATTAATCCGTCGAg < 1:841922/68‑1 (MQ=255)tGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACAAAATTAATCCGTCGAg < 1:537641/68‑1 (MQ=255)tGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACAAAATTAATCCGTCGAg < 1:1678125/68‑1 (MQ=255)tGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACAAAATTAATCCGTCGAg < 1:2729991/68‑1 (MQ=255) tctGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACAAAATTAATCCGTCGAGGATTGCGCTTTTTTCTg > 1:3336022/1‑69 (MQ=255) tctGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACAAAATTAATCCGTCGAGGATTGCGCTTTTTTCTg > 1:1811530/1‑69 (MQ=255) ctgTTTTCCAGAAAAGGGAGTGAAGAGAAACAAAATTAATCTGTc < 1:3153489/45‑1 (MQ=38) ctgTTTTCCAGAAAAGGGAGTGAAGAGAAACAAAATTAATCTGTc < 1:211994/45‑1 (MQ=38) ctgTTTTCCAGAAAAGGGAGTGAAGAGAAACAAAATTAATCTGTc < 1:2253356/45‑1 (MQ=38) ctgTTTTCCAGAAAAGGGAGTGAAGAGAAACAAAATTAATCTGTc < 1:1930744/45‑1 (MQ=38) ctgTTTTCCAGAAAAGGGAGTGAAGAGAAACAAAATTAATCTGTc < 1:670330/45‑1 (MQ=38) ctgTTTTCCAGAAAAGGGAGTGAAGAGAAACAAAATTAATCTGTc < 1:699510/45‑1 (MQ=38) ctgTTTTCCAGAAAAGGGAGTGAAGAGAAACAAAATTAATCTGTc < 1:857474/45‑1 (MQ=38) ctgTTTTCCAGAAAAGGGAGTGAAGAGAAACAAAATTAATCTGTc < 1:980540/45‑1 (MQ=38) ctgTTTTCCAGAAAAGGGAGTGAAGAGAAACAAAATTAATCTGTc < 1:1051654/45‑1 (MQ=38) gTTTTCCAGGAAAGGGAGTGAAGAGAAACAAAATTAATCCGTCGAGGATTGCGCTTTTTTCTGCGCttt < 1:2679933/69‑1 (MQ=255) ttCCAGGAAAGGGAGTGAAGAGAAACAAAATTAATCCGTCGAGGATTGCGCTTTTTTCTGCGCttt < 1:261681/66‑1 (MQ=255) ttCCAGGAAAGGGAGTGAAGAGAAACAAAATTAATCAGTCGAGGATTGCGCTTTTTTCTGCGCTTTAAt > 1:2436451/1‑69 (MQ=255) ccAGGAAAGGGAGTGAAGAGAAACAAAATTAATCCGTCGAGGATTGCGCTTTTTTCTGCGCTTTAAtt < 1:3232396/68‑1 (MQ=255) ccAGGAAAGGGAGTGAAGAGAAACAAAATTAATCCGTCGAGGATTGCGCTTTTTTCTGCGCTTTAAtt < 1:2832756/68‑1 (MQ=255) ccAGGAAAGGGAGTGAAGAGAAACAAAATTAATCCGTCGAGGATTGCGCTTTTTTCTGCGCTTTAAtt < 1:1025617/68‑1 (MQ=255) ccAGGAAAGGGAGTGAAGAGAAACAAAATTAATCCGTCGAGGATTGCGCTTTTTTCTGCGCTTTAAtt < 1:3342482/68‑1 (MQ=255) gAGTGAAGAGAAACAAAATTAATCCGTCGAGGATTGCGCTTTTTTCTGCGCTTTAATTTCCTGgcggcg < 1:39728/69‑1 (MQ=255) gAGTGAAGAGAAACAAAATTAATCCGTCGAGGATTGCGCTTTTTTCTGCGCTTTAATTTCCTGgcggcg < 1:2414909/69‑1 (MQ=255) gAGTGAAGAGAAACAAAATTAATCCGTCGAGGATTGCGCTTTTTTCTGCGCTTTAATTTCCTGgcggcg < 1:462008/69‑1 (MQ=255) gAGTGAAGAGAAACAAAATTAATCCGTCGAGGATTGCGCTTTTTTCTGCGCTTTAATTTCCTGgcggcg < 1:2455229/69‑1 (MQ=255) gAGTGAAGAGAAACAAAATTAATCCGTCGAGGATTGCGCTTTTTTCTGCGCTTTAATTTCCTGgcggcg < 1:506663/69‑1 (MQ=255) gAGTGAAGAGAAACAAAATTAATCCGTCGAGGATTGCGCTTTTTTCTGCGCTTTAATTTCCTGgcggcg < 1:606085/69‑1 (MQ=255) gAGTGAAGAGAAACAAAATTAATCCGTCGAGGATTGCGCTTTTTTCTGCGCTTTAATTTCCTGgcggcg < 1:1647907/69‑1 (MQ=255) gAGTGAAGAGAAACAAAATTAATCCGTCGAGGATTGCGCTTTTTTCTGCGCTTTAATTTCCTGgcggcg < 1:2737661/69‑1 (MQ=255) gAGTGAAGAGAAACAAAATTAATCCGTCGAGGATTGCGCTTTTTTCTGCGCTTTAATTTCCTGgcggcg < 1:70005/69‑1 (MQ=255) gAGTGAAGAGAAACAAAATTAATCCGTCGAGGATTGCGCTTTTTTCTGCGCTTTAATTTCCTGgcggcg > 1:320792/1‑69 (MQ=255) aGTGAAGAGAAACAAAATTAATCCGTCGAGGATTGCGCTTTTTTCTGCGCTTTAAtt < 1:456379/57‑1 (MQ=255) gTGAAGAGAAACAAAATTAATCCGTCGAGGATTGCGCTTTTTTCTGCGCTTTAATTTCCTGgcggcg > 1:3302745/1‑67 (MQ=255) gTGAAGAGAAACAAAATTAATCCGTCGAGGATTGCGCTTTTTTCTGCGCTTTAATTTCCTGgcggcg > 1:650743/1‑67 (MQ=255) gTGAAGAGAAACAAAATTAATCCGTCGAGGATTGCGCTTTTTTCTGCGCTTTAATTTCCTGgcggcg > 1:2884853/1‑67 (MQ=255) gTGAAGAGAAACAAAATTAATCCGTCGAGGATTGCGCTTTTTTCTGCGCTTTAATTTCCTGgcggcg > 1:1264884/1‑67 (MQ=255) gagaAACAAAATTAATCCGTCGAGGATTGCGCTTTTTTCTGCGCTTTAATTTCCTGGCGGCGCATGCg < 1:1051742/68‑1 (MQ=255) gagaAACAAAATTAATCCGTCGAGGATTGCGCTTTTTTCTGCGCTTTAATTTCCTGGCGGCGCATGCg < 1:2651953/68‑1 (MQ=255) agaAACAAAATTAATCTGTCGAGGATTGCGCTTTTTTCTGCGCTTTAATTTCCTGGCGGCGCATGc > 1:514009/1‑66 (MQ=255) agaAACAAAATTAATCTGTCGAGGATTGCGCTTTTTTCTGCGCTTTAATTTCCTGGCGGCGCATGc > 1:998077/1‑66 (MQ=255) agaAACAAAATTAATCTGTCGAGGATTGCGCTTTTTTCTGCGCTTTAATTTCCTGGCGGCGCATGc > 1:951599/1‑66 (MQ=255) agaAACAAAATTAATCTGTCGAGGATTGCGCTTTTTTCTGCGCTTTAATTTCCTGGCGGCGCATGc > 1:1224532/1‑66 (MQ=255) agaAACAAAATTAATCTGTCGAGGATTGCGCTTTTTTCTGCGCTTTAATTTCCTGGCGGCGCATGc > 1:722956/1‑66 (MQ=255) agaAACAAAATTAATCTGTCGAGGATTGCGCTTTTTTCTGCGCTTTAATTTCCTGGCGGCGCATGc > 1:12949/1‑66 (MQ=255) agaAACAAAATTAATCTGTCGAGGATTGCGCTTTTTTCTGCGCTTTAATTTCCTGGCGGCGCATGc > 1:1692346/1‑66 (MQ=255) agaAACAAAATTAATCTGTCGAGGATTGCGCTTTTTTCTGCGCTTTAATTTCCTGGCGGCGCATGc > 1:524817/1‑66 (MQ=255) agaAACAAAATTAATCTGTCGAGGATTGCGCTTTTTTCTGCGCTTTAATTTCCTGGCGGCGCATGc > 1:3074446/1‑66 (MQ=255) agaAACAAAATTAATCTGTCGAGGATTGCGCTTTTTTCTGCGCTTTAATTTCCTGGCGGCGCATGc > 1:1777042/1‑66 (MQ=255) agaAACAAAATTAATCTGTCGAGGATTGCGCTTTTTTCTGCGCTTTAATTTCCTGGCGGCGCATGc > 1:471490/1‑66 (MQ=255) agaAACAAAATTAATCTGTCGAGGATTGCGCTTTTTTCTGCGCTTTAATTTCCTGGCGGCGCATGc > 1:1955336/1‑66 (MQ=255) agaAACAAAATTAATCTGTCGAGGATTGCGCTTTTTTCTGCGCTTTAATTTCCTGGCGGCGCATGc > 1:1015563/1‑66 (MQ=255) agaAACAAAATTAATCTGTCGAGGATTGCGCTTTTTTCTGCGCTTTAATTTCCTGGCGGCGCATGc > 1:3402374/1‑66 (MQ=255) agaAACAAAATTAATCTGTCGAGGATTGCGCTTTTTTCTGCGCTTTAATTTCCTGGCGGCGCATGc > 1:2274894/1‑66 (MQ=255) agaAACAAAATTAATCTGTCGAGGATTGCGCTTTTTTCTGCGCTTTAATTTCCTGGCGGCGCATGCGa > 1:1938/1‑68 (MQ=255) gaAACAAAATTAATCCGTCGAGGATTGCGCTTTTTTCTGCGCTTTAATTTCCTGGCGGCGCATGCGaa > 1:2006972/1‑68 (MQ=255) gaAACAAAATTAATCCGTCGAGGATTGCGCTTTTTTCTGCGCTTTAATTTCCTGGCGGCGCATGCGaa > 1:534882/1‑68 (MQ=255) gaAACAAAATTAATCCGTCGAGGATTGCGCTTTTTTCTGCGCTTTAATTTCCTGGCGGCGCATGCGaa > 1:2083496/1‑68 (MQ=255) gaAACAAAATTAATCCGTCGAGGATTGCGCTTTTTTCTGCGCTTTAATTTCCTGGCGGCGCATGCGa > 1:3397006/1‑67 (MQ=255) aaaaTTAATCCGTCGAGGATTGCGCTTTTTTCTGCGCTTTAATTTCCTGGCGGCGCATGCGAATGAAGt < 1:1392962/69‑1 (MQ=255) aaaaTTAATCCGTCGAGGATTGCGCTTTTTTCTGCGCTTTAATTTCCTGGCGGCGCATGCGAAAGGAGt < 1:1731192/69‑1 (MQ=255) aaaaTTAATCCGTCGAGGATTGCGCTTTTTTCTGCGCTTTAATTTCCTGGCGGCGCATGCGAAAGAAGt < 1:1501227/69‑1 (MQ=255) aaaaTTAATCCGTCGAGGATTGCGCTTTTTTCTGCGCTTTAATTTCCTGGCGGCGCATGCGAAAGAAGt < 1:322766/69‑1 (MQ=255) aaaaTTAATCCGTCGAGGATTGCGCTTTTTTCTGCGCTTTAATTTCCTGGCGGCGCATGCGAAAGAAGt < 1:1090311/69‑1 (MQ=255) aaaaTTAATCCGTCGAGGATTGCGCTTTTTTCTGCGCTTTAATTTCCTGGCGGCGCATGCGAAAGAAGt < 1:2636531/69‑1 (MQ=255) aaaaTTAATCCGTCGAGGATTGCGCTTTTTTCTGCGCTTTAATTTCCTGGCGGCGCATGCGAAAGAAGt < 1:1643687/69‑1 (MQ=255) aaaaTTAATCCGTCGAGGATTGCGCTTTTTTCTGCGCTTTAATTTCCTGGCGGCGCATGCGAAAGAAGt < 1:613094/69‑1 (MQ=255) aaaaTTAATCCGTCGAGGATTGCGCTTTTTTCTGCGCTTTAATTTCCTGGCGGCGCATGCGAAAGAAGt < 1:3415783/69‑1 (MQ=255) aaaTTAATCCGTCGAGGATTGCGCTTTTTTCTGCGCTTTAATTTCCTGGCGGCGCATGCGAAAGAAGt < 1:3192883/68‑1 (MQ=255) | TGACCCATTCTCCCTCTCTGCTGTTTTCCAGGAAAGGGAGTGAAGAGAAACAAAATTAATCCGTCGAGGATTGCGCTTTTTTCTGCGCTTTAATTTCCTGGCGGCGCATGCGAAAGAAGT > minE/1729985‑1730104 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |