| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,732,510 | C→T | 51.7% | C54C (TGC→TGT) | yfhL → | predicted 4Fe‑4S cluster‑containing protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,732,510 | 0 | C | T | 51.7% | 1.3 / 106.0 | 116 | C54C (TGC→TGT) | yfhL | predicted 4Fe‑4S cluster‑containing protein |
| Reads supporting (aligned to +/- strand): ref base C (11/45); new base T (22/38); total (33/83) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.31e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 121 total aligned reads displayed.
GCGATAAGTGTACCGAATGCGTAGGGCACTACGAGACACCAACCTGCCAGAAGGTGTGCCCGATCCCCAATACTATTGTGAAAGATCCGGCGCATGTCGAGACAGAAGAACAGTTGTGGGATAAATT > minE/1732452‑1732578 | gagATAAGTGTACCGAATGCGTAGGGCACTACGAGACACCAACCTGCCAGAAGGTGTGCCCGATcccc > 1:1424779/3‑68 (MQ=255)gCGATAAGTGTACCGAATGCGTAGGGCACTACGAGACACCAACCTGCCAGAAGGTGTGCCCGATcccc > 1:2432647/1‑68 (MQ=255)gCGATAAGTGTACCGAATGCGTAGGGCACTACGAGACACCAACCTGCCAGAAGGTGTGCCCGATcccc > 1:457114/1‑68 (MQ=255)gCGATAAGTGTACCGAATGCGTAGGGCACTACGAGACACCAACCTGCCAGAAGGTGTGCCCGATcccc > 1:1042531/1‑68 (MQ=255) cGATAAGTGTACCGAATGCGTAGGGTACTACGAGACACCAACCTGCCAGAAGGTGTGCCCGATCCCCaa < 1:1331282/69‑1 (MQ=255) cGATAAGTGTACCGAATGCGTAGGGCACTACGAGACACCAACCTGCCAGAAGGTGTGCCCGATCCCCaa < 1:3097363/69‑1 (MQ=255) cGATAAGTGTACCGAATGCGTAGGGCACTACGAGACACCAACCTGCCAGAAGGTGTGCCCGATCCCCaa < 1:3069406/69‑1 (MQ=255) cGATAAGTGTACCGAATGCGTAGGGCACTACGAGACACCAACCTGCCAGAAGGTGTGCCCGATCCCCaa < 1:2836444/69‑1 (MQ=255) cGATAAGTGTACCGAATGCGTAGGGCACTACGAGACACCAACCTGCCAGAAGGTGTGCCCGATCCCCaa < 1:2724722/69‑1 (MQ=255) cGATAAGTGTACCGAATGCGTAGGGCACTACGAGACACCAACCTGCCAGAAGGTGTGCCCGATCCCCaa < 1:1014846/69‑1 (MQ=255) cGATAAGTGTACCGAATGCGTAGGGCACTACGAGACACCAACCTGCCAGAAGGTGTGCCCGATCCCCaa < 1:2320971/69‑1 (MQ=255) cGATAAGTGTACCGAATGCGTAGGGCACTACGAGACACCAACCTGCCAGAAGGTGTGCCCGATCCCCaa < 1:2286505/69‑1 (MQ=255) cGATAAGTGTACCGAATGCGTAGGGCACTACGAGACACCAACCTGCCAGAAGGTGTGCCCGATCCCCaa < 1:2229719/69‑1 (MQ=255) cGATAAGTGTACCGAATGCGTAGGGCACTACGAGACACCAACCTGCCAGAAGGTGTGCCCGATCCCCaa < 1:2180247/69‑1 (MQ=255) cGATAAGTGTACCGAATGCGTAGGGCACTACGAGACACCAACCTGCCAGAAGGTGTGCCCGATCCCCaa < 1:2165420/69‑1 (MQ=255) cGATAAGTGTACCGAATGCGTAGGGCACTACGAGACACCAACCTGCCAGAAGGTGTGCCCGATCCCCaa < 1:1682945/69‑1 (MQ=255) cGATAAGTGTACCGAATGCGTAGGGCACTACGAGACACCAACCTGCCAGAAGGTGTGCCCGATCCCCaa < 1:3404612/69‑1 (MQ=255) cGATAAGTGTACCGAATGCGTAGGGCACTACGAGACACCAACCTGCCAGAAGGTGTGCCCGATCCCCaa < 1:1042786/69‑1 (MQ=255) cGATAAGTGTACCGAATGCGTAGGGCACTACGAGACACCAACCTGCCAGAAGGTGTGCCCGATCCCCaa < 1:140544/69‑1 (MQ=255) cGATAAGTGTACCGAATGCGTAGGGCACTACGAGACACCAACCTGCCAGAAGGTGTGCCCGATCCCCaa < 1:787710/69‑1 (MQ=255) gATAAGTGTACCGAATGCGTAGGGCACTACGAGACACCAACCTGCCAGAAGGTGTGCCCGATCCCCaa < 1:780989/68‑1 (MQ=255) aGTGTACCGAATGCGTAGGGCACTACGAGACACCAACCTGCCAGAAGGTGTGCCCGATCCCCAATACTa < 1:619965/69‑1 (MQ=255) aGTGTACCGAATGCGTAGGGCACTACGAGACACCAACCTGCCAGAAGGTGTGCCCGATCCCCAATACTa < 1:3235099/69‑1 (MQ=255) aGTGTACCGAATGCGTAGGGCACTACGAGACACCAACCTGCCAGAAGGTGTGCCCGATCCCCAATACTa < 1:2985807/69‑1 (MQ=255) aGTGTACCGAATGCGTAGGGCACTACGAGACACCAACCTGCCAGAAGGTGTGCCCGATCCCCAATACTa < 1:1997262/69‑1 (MQ=255) aGTGTACCGAATGCGTAGGGCACTACGAGACACCAACCTGCCAGAAGGTGTGCCCGATCCCCAATACTa < 1:706779/69‑1 (MQ=255) aGTGTACCGAATGCGTAGGGCACTACGAGACACCAACCTGCCAGAAGGTGTGCCCGATCCCCAATACTa < 1:753005/69‑1 (MQ=255) ccGAATGCGTAGGGCACTACGAGACACCAACCTGCCAGAAGGTGTGCCCGATCCCCAATACTATtgt > 1:2557906/1‑67 (MQ=255) ccGAATGCGTAGGGCACTACGAGACACCAACCTGCCAGAAGGTGTGCCCGATCCCCAATACTATtgt > 1:2115005/1‑67 (MQ=255) tGCGTAGGGCACTACGAGACACCAACCTGCCAGAAGGTGTGTCCGATCCCCAATACTATTGTGAAAGa > 1:1781979/1‑68 (MQ=255) tGCGTAGGGCACTACGAGACACCAACCTGCCAGAAGGTGTGTCCGATCCCCAATACTATTGTGAAAGa > 1:34738/1‑68 (MQ=255) tGCGTAGGGCACTACGAGACACCAACCTGCCAGAAGGTGTGTCCGATCCCCAATACTATTGTGAAAGAt > 1:3052527/1‑69 (MQ=255) tGCGTAGGGCACTACGAGACACCAACCTGCCAGAAGGTGTGTCCGATCCCCAATACTATTGTGAAAGAt > 1:2669218/1‑69 (MQ=255) tGCGTAGGGCACTACGAGACACCAACCTGCCAGAAGGTGTGTCCGATCCCCAATACTATTGTGAAAGAt > 1:2609018/1‑69 (MQ=255) tGCGTAGGGCACTACGAGACACCAACCTGCCAGAAGGTGTGTCCGATCCCCAATACTATTGTGAAAGAt > 1:1149410/1‑69 (MQ=255) tGCGTAGGGCACTACGAGACACCAACCTGCCAGAAGGTGTGTCCGATCCCCAATACTATTGTGAAAGAt > 1:1113076/1‑69 (MQ=255) tGCGTAGGGCACTACGAGACACCAACCTGCCAGAAGGTGTGTCCGATCCCCAATACTATTGTGAAAGAt > 1:2840716/1‑69 (MQ=255) tGCGTAGGGCACTACGAGACACCAACCTGCCAGAAGGTGTGTCCGATCCCCAATACTATTGTGAAAGAt > 1:381207/1‑69 (MQ=255) tGCGTAGGGCACTACGAGACACCAACCTGCCAGAAGGTGTGTCCGATCCCCAATACTATTGTGAAAGAt > 1:285441/1‑69 (MQ=255) tGCGTAGGGCACTACGAGACACCAACCTGCCAGAAGGTGTGTCCGATCCCCAATACTATTGTGAAAGAt > 1:2903667/1‑69 (MQ=255) tGCGTAGGGCACTACGAGACACCAACCTGCCAGAAGGTGTGTCCGATCCCCAATACTATTGTGAAAGAt > 1:1573211/1‑69 (MQ=255) tGCGTAGGGCACTACGAGACACCAACCTGCCAGAAGGTGTGTCCGATCCCCAATACTATTGTGAAAGAt > 1:1236066/1‑69 (MQ=255) tGCGTAGGGCACTACGAGACACCAACCTGCCAGAAGGTGTGTCCGATCCCCAATACTATTGTGAAAGAt > 1:1499753/1‑69 (MQ=255) tGCGTAGGGCACTACGAGACACCAACCTGCCAGAAGGTGTGTCCGATCCCCAATACTATTGTGAAAGAt > 1:2121251/1‑69 (MQ=255) tGCGTAGGGCACTACGAGACACCAACCTGCCAGAAGGTGTGTCCGATCCCCAATACTATTGTGAAAGAt > 1:1990594/1‑69 (MQ=255) tGCGTAGGGCACTACGAGACACCAACCTGCCAGAAGGTGTGTCCGATCCCCAATACGATTGTGAAAGa > 1:2724172/1‑68 (MQ=255) tGCGTAGGGCACTACGAGACA‑CAACCTGCCAGAAGGTGTGTCCGATCCCCAATACTATTGTGAAAGATc > 1:2128009/1‑69 (MQ=255) tAGGGCACTACGAGACACCAACCTGCCAGAAGGTGTGCCCGATCCCCAATACTATTGTGAAAGATCCgg < 1:876990/69‑1 (MQ=255) tAGGGCACTACGAGACACCAACCTGCCAGAAGGTGTGCCCGATCCCCAATACTATTGTGAAAGATCCgg < 1:6412/69‑1 (MQ=255) aGGGCACTACGAGACACCAACCTGCCAGAAGGGGTGCCCGATCCCCAATACTATTGTGAAAGATCCgg < 1:1066995/68‑1 (MQ=255) agaCACCAACCTGCCAGAAGGTGTGTCCGATCCCCAATACTATTGTGAAAGATCCGCAACATGTCga < 1:2525163/67‑1 (MQ=255) agaCACCAACCTGCCAGAAGGTGTGTCCGATCCCCAATACTATTGTGAAAGATCCGCAACATGTCga < 1:3170353/67‑1 (MQ=255) agaCACCAACCTGCCAGAAGGTGTGTCCGATCCCCAATACTATTGTGAAAGATCCGCAACATGTCga < 1:3390002/67‑1 (MQ=255) agaCACCAACCTGCCAGAAGGTGTGTCCGATCCCCAATACTATTGTGAAAGATCCGCAACATGTCga < 1:3338156/67‑1 (MQ=255) agaCACCAACCTGCCAGAAGGTGTGTCCGATCCCCAATACTATTGTGAAAGATCCGCAACATGTCga < 1:272366/67‑1 (MQ=255) agaCACCAACCTGCCAGAAGGTGTGTCCGATCCCCAATACTATTGTGAAAGATCCGCAACATGTCga < 1:2693052/67‑1 (MQ=255) agaCACCAACCTGCCAGAAGGTGTGTCCGATCCCCAATACTATTGTGAAAGATCCGCAACATGTCga < 1:1473501/67‑1 (MQ=255) agaCACCAACCTGCCAGAAGGTGTGTCCGATCCCCAATACTATTGTGAAAGATCCGCAACATGTCga < 1:62986/67‑1 (MQ=255) agaCACCAACCTGCCAGAAGGTGTGTCCGATCCCCAATACTATTGTGAAAGATCCGCAACATGTCga < 1:1827618/67‑1 (MQ=255) agaCACCAACCTGCCAGAAGGTGTGTCCGATCCCCAATACTATTGTGAAAGATCCGCAACATGTCga < 1:646311/67‑1 (MQ=255) agaCACCAACCTGCCAGAAGGTGTGTCCGATCCCCAATACTATTGTGAAAGATCCGCAACATGTCga < 1:1767545/67‑1 (MQ=255) agaCACCAACCTGCCAGAAGGTGTGTCCGATCCCCAATACTATTGTGAAAGATCCGCAACATGTCga < 1:2053522/67‑1 (MQ=255) agaCACCAACCTGCCAGAAGGTGTGTCCGATCCCCAATACTATTGTGAAAGATCCGCAACATGTCga < 1:2090569/67‑1 (MQ=255) agaCACCAACCTGCCAGAAGGTGTGTCCGATCCCCAATACTATTGTGAAAGATCCGCAACATGTCga < 1:218038/67‑1 (MQ=255) agaCACCAACCTGCCAGAAGGTGTGTCCGATCCCCAATACTATTGTGAAAGATCCGCAACATGTCga < 1:2186609/67‑1 (MQ=255) agaCACCAACCTGCCAGAAGGTGTGTCCGATCCCCAATACTATTGTGAAAGATCCGCAACATGTCga < 1:266273/67‑1 (MQ=255) agaCACCAACCTGCCAGAAGGTGTGTCCGATCCCCAATACTATTGTGAAAGATCCGCAACATGTCga < 1:227457/67‑1 (MQ=255) agaCACCAACCTGCCAGAAGGTGTGTCCGATCCCCAATACTATTGTGAAAGATCCGCAACATGTCga < 1:814290/67‑1 (MQ=255) agaCACCAACCTGCCAGAAGGTGTGTCCGATCCCCAATACTATTGTGAAAGATCCGCAACATGTCga < 1:1411510/67‑1 (MQ=255) agaCACCAACCTGCCAGAAGGTGTGTCCGATCCCCAATACTATTGTGAAAGATCCGCAACATGTCga < 1:92277/67‑1 (MQ=255) agaCACCAACCTGCCAGAAGGTGTGTCCGATCCCCAATACTATTGTGAAAGATCCGCAACATGTCga < 1:2441018/67‑1 (MQ=255) agaCACCAACCTGCCAGAAGGTGTGTCCGATCCCCAATACTATTGTGAAAGATCCGCAACATGTCga < 1:2456072/67‑1 (MQ=255) agaCACCAACCTGCCAGAAGGTGTGTCCGATCCCCAATACTATTGTGAAAGATCCGCAACATGTCga < 1:1805933/67‑1 (MQ=255) agaCACCAACCTGCCAGAAGGTGTGTCCGATCCCCAATACTATTGTGAAAGATCCGCAACATGTCga < 1:1152455/67‑1 (MQ=255) cacCAACCTGCCAGAAGGTGTGCCCGATCCCCAATACTATTGTGAAAGATCCGGCGCa < 1:762690/58‑1 (MQ=255) cacCAACCTGCCAGAAGGTGTGCCCGATCCCCAATACTATTGTGAAAGATCCGGCGCa < 1:970849/58‑1 (MQ=255) cacCAACCTGCCAGAAGGTGTGCCCGATCCCCAATACTATTGTGAAAGATCCGGCGCa < 1:417109/58‑1 (MQ=255) cacCAACCTGCCAGAAGGTGTGCCCGATCCCCAATACTATTGTGAAAGATCCGGCGCa < 1:1358539/58‑1 (MQ=255) cacCAACCTGCCAGAAGGTGTGCCCGATCCCCAATACTATTGTGAAAGATCCGGCGCa < 1:3180204/58‑1 (MQ=255) cacCAACCTGCCAGAAGGTGTGCCCGATCCCCAATACTATTGTGAAAGATCCGGCGCa < 1:2844884/58‑1 (MQ=255) aaCCTGCCAGAAGGTGTGCCCGATCCCCAATACTATTGTGAAAGATCCGGCGCATGTCGAGACagaag > 1:2100144/1‑68 (MQ=255) aCCTGCCAGAAGGTGTGCCCGATCCCCAATACTATTGTGAAAGATCCGGCGCATGTCGAGACAgaagaa < 1:3338989/69‑1 (MQ=255) aCCTGCCAGAAGGTGTGCCCGATCCCCAATACTATTGTGAAAGATCCGGCGCATGTCGAGACAgaagaa < 1:1701575/69‑1 (MQ=255) aGAAGGTGTGTCCGATCCCCAATACTATTGTGAAAGATCCGCAACATGTCGAGACGGAAGAACAGtt < 1:2611525/67‑1 (MQ=255) aGAAGGTGTGTCCGATCCCCAATACTATTGTGAAAGATCCGCAACATGTCGAGACGGAAGAACAGtt < 1:1014894/67‑1 (MQ=255) aGAAGGTGTGTCCGATCCCCAATACTATTGTGAAAGATCCGCAACATGTCGAGACGGAAGAACAGtt < 1:115549/67‑1 (MQ=255) aGAAGGTGTGTCCGATCCCCAATACTATTGTGAAAGATCCGCAACATGTCGAGACGGAAGAACAGtt < 1:319714/67‑1 (MQ=255) aGAAGGTGTGTCCGATCCCCAATACTATTGTGAAAGATCCGCAACATGTCGAGACGGAAGAACAGtt < 1:1828428/67‑1 (MQ=255) aGAAGGTGTGTCCGATCCCCAATACTATTGTGAAAGATCCGCAACATGTCGAGACGGAAGAACAGtt < 1:1952982/67‑1 (MQ=255) aGAAGGTGTGTCCGATCCCCAATACTATTGTGAAAGATCCGCAACATGTCGAGACGGAAGAACAGtt < 1:3101962/67‑1 (MQ=255) aGAAGGTGTGTCCGATCCCCAATACTATTGTGAAAGATCCGCAACATGTCGAGACGGAAGAACAGtt < 1:2393860/67‑1 (MQ=255) aGAAGGTGTGTCCGATCCCCAATACTATTGTGAAAGATCCGCAACATGTCGAGACGGAAGAACAGtt < 1:2629372/67‑1 (MQ=255) aaGGTGTGCCCGATCCCCAATACTATTGTGAAAGATCCgg < 1:168728/40‑1 (MQ=255) tgtgCCCGATCCCCAATACTATTGTGAAAGATCCGGCGCATGTCGAGACAGAAGAACAGTTGTGGGATa < 1:2264571/69‑1 (MQ=255) tgtgCCCGATCCCCAATACTATTGTGAAAGATCCGGCGCATGTCGAGACAGAAGAACAGTTGTGGGATa < 1:3154449/69‑1 (MQ=255) tgtgCCCGATCCCCAATACTATTGTGAAAGATCCGGCGCATGTCGAGACAGAAGAACAGTTGTGGGATa < 1:122750/69‑1 (MQ=255) gtgCCCGATCCCCAATACTATTGTTAAAGATCCGGCGCATGTCGAGACAGAAGAACAGTTGTGGGATa < 1:896779/68‑1 (MQ=255) cccGATCCCCAATACTATTGTGAAAGATCCGGCGCATGTCGAGACAGAAGAACAGTTGTGGGATAAAtt > 1:1296139/1‑69 (MQ=255) cccGATCCCCAATACTATTGTGAAAGATCCGGCGCATGTCGAGACAGAAGAACAGTTGTGGGATAAAtt > 1:1190798/1‑69 (MQ=255) cccGATCCCCAATACTATTGTGAAAGATCCGGCGCATGTCGAGACAGAAGAACAGTTGTGGGATAAAtt > 1:502907/1‑69 (MQ=255) | GCGATAAGTGTACCGAATGCGTAGGGCACTACGAGACACCAACCTGCCAGAAGGTGTGCCCGATCCCCAATACTATTGTGAAAGATCCGGCGCATGTCGAGACAGAAGAACAGTTGTGGGATAAATT > minE/1732452‑1732578 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |