| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,732,555 | A→G | 48.9% | T69T (ACA→ACG) | yfhL → | predicted 4Fe‑4S cluster‑containing protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,732,555 | 0 | A | G | 48.9% | ‑5.2 / 85.3 | 92 | T69T (ACA→ACG) | yfhL | predicted 4Fe‑4S cluster‑containing protein |
| Reads supporting (aligned to +/- strand): ref base A (35/12); new base G (30/15); total (65/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.94e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.23e-01 | |||||||||||
AACCTGCCAGAAGGTGTGCCCGATCCCCAATACTATTGTGAAAGATCCGGCGCATGTCGAGACAGAAGAACAGTTGTGGGATAAATTTGTGCTGATGCACCACGCGGATAAAATTTAATATTCTA > minE/1732492‑1732616 | aaCCTGCCAGAAGGTGTGCCCGATCCCCAATACTATTGTGAAAGATCCGGCGCATGTCGAGACagaag > 1:2100144/1‑68 (MQ=255)aaCCTGCCAGAAGGTGTGCCCGATCCCCAATACTATTGTGAAAGATCCGGCGCATGTCGAGACagaag > 1:2096205/1‑68 (MQ=255) aCCTGCCAGAAGGTGTGCCCGATCCCCAATACTATTGTGAAAGATCCGGCGCATGTCGAGACAgaagaa < 1:3338989/69‑1 (MQ=255) aCCTGCCAGAAGGTGTGCCCGATCCCCAATACTATTGTGAAAGATCCGGCGCATGTCGAGACAgaagaa < 1:1701575/69‑1 (MQ=255) aGAAGGTGTGTCCGATCCCCAATACTATTGTGAAAGATCCGCATCATGTCGAGACGGAAGAACAGtt < 1:3293723/67‑1 (MQ=255) aGAAGGTGTGTCCGATCCCCAATACTATTGTGAAAGATCCGCAACATGTCGAGACGGAAGAACAGtt < 1:2629372/67‑1 (MQ=255) aGAAGGTGTGTCCGATCCCCAATACTATTGTGAAAGATCCGCAACATGTCGAGACGGAAGAACAGtt < 1:2611525/67‑1 (MQ=255) aGAAGGTGTGTCCGATCCCCAATACTATTGTGAAAGATCCGCAACATGTCGAGACGGAAGAACAGtt < 1:746035/67‑1 (MQ=255) aGAAGGTGTGTCCGATCCCCAATACTATTGTGAAAGATCCGCAACATGTCGAGACGGAAGAACAGtt < 1:1828428/67‑1 (MQ=255) aGAAGGTGTGTCCGATCCCCAATACTATTGTGAAAGATCCGCAACATGTCGAGACGGAAGAACAGtt < 1:2410450/67‑1 (MQ=255) aGAAGGTGTGTCCGATCCCCAATACTATTGTGAAAGATCCGCAACATGTCGAGACGGAAGAACAGtt < 1:1919365/67‑1 (MQ=255) aGAAGGTGTGTCCGATCCCCAATACTATTGTGAAAGATCCGCAACATGTCGAGACGGAAGAACAGtt < 1:1952982/67‑1 (MQ=255) aGAAGGTGTGTCCGATCCCCAATACTATTGTGAAAGATCCGCAACATGTCGAGACGGAAGAACAGtt < 1:319714/67‑1 (MQ=255) aGAAGGTGTGTCCGATCCCCAATACTATTGTGAAAGATCCGCAACATGTCGAGACGGAAGAACAGtt < 1:2393860/67‑1 (MQ=255) aGAAGGTGTGTCCGATCCCCAATACTATTGTGAAAGATCCGCAACATGTCGAGACGGAAGAACAGtt < 1:3101962/67‑1 (MQ=255) aGAAGGTGTGTCCGATCCCCAATACTATTGTGAAAGATCCGCAACATGTCGAGACGGAAGAACAGtt < 1:115549/67‑1 (MQ=255) aGAAGGTGTGTCCGATCCCCAATACTATTGTGAAAGATCCGCAACATGTCGAGACGGAAGAACAGtt < 1:1014894/67‑1 (MQ=255) tgtgCCCGATCCCCAATACTATTGTGAAAGATCCGGCGCATGTCGAGACAGAAGAACAGTTGTGGGATa < 1:2264571/69‑1 (MQ=255) tgtgCCCGATCCCCAATACTATTGTGAAAGATCCGGCGCATGTCGAGACAGAAGAACAGTTGTGGGATa < 1:3154449/69‑1 (MQ=255) tgtgCCCGATCCCCAATACTATTGTGAAAGATCCGGCGCATGTCGAGACAGAAGAACAGTTGTGGGATa < 1:122750/69‑1 (MQ=255) gtgCCCGATCCCCAATACTATTGTTAAAGATCCGGCGCATGTCGAGACAGAAGAACAGTTGTGGGATa < 1:896779/68‑1 (MQ=255) cccGATCCCCAATACTATTGTGAAAGATCCGGCGCATGTCGAGACAGAAGAACAGTTGTGGGATAAAtt > 1:1296139/1‑69 (MQ=255) cccGATCCCCAATACTATTGTGAAAGATCCGGCGCATGTCGAGACAGAAGAACAGTTGTGGGATAAAtt > 1:502907/1‑69 (MQ=255) cccGATCCCCAATACTATTGTGAAAGATCCGGCGCATGTCGAGACAGAAGAACAGTTGTGGGATAAAtt > 1:3426671/1‑69 (MQ=255) cccGATCCCCAATACTATTGTGAAAGATCCGGCGCATGTCGAGACAGAAGAACAGTTGTGGGATAAAtt > 1:1190798/1‑69 (MQ=255) cccGATCCCCAATACTATTGTGAAAGATACGGCGCATGTCGAGACAGAAGAACAGTTGTGGGATAAAtt > 1:1733942/1‑69 (MQ=255) cGATCCCCAATACTATTGTGAAAGATCCGGCGCATGTCGAGACAGAAGAACAGTTGTGGGATAAATTtg < 1:1442094/69‑1 (MQ=255) cGATCCCCAATACTATTGTGAAAGATCCGGCGCATGTCGAGACAGAAGAACAGTTGTGGGATAAATTtg < 1:2076326/69‑1 (MQ=255) cGATCCCCAATACTATTGTGAAAGATCCGGCGCATGTCGAGACAGAAGAACAGTTGTGGGATAAATTtg < 1:908217/69‑1 (MQ=255) cGATCCCCAATACTATTGTGAAAGATCCGGCGCATGTCGAGACAGAAGAACAGTTGTGGGATAAATTtg < 1:2578891/69‑1 (MQ=255) cGATCCCCAATACTATTGTGAAAGATCCGGCGCATGTCGAGACAGAAGAACAGTTGTGGGATAAATTtg < 1:2908975/69‑1 (MQ=255) ccccAATACTATTGTGAAAGATCCGGCGCATGTCGAGACAGAAGAACAGTTGTGGGATAAATTTGTGCt > 1:2125032/1‑69 (MQ=255) ccccAATACTATTGTGAAAGATCCGGCGCATGTCGAGACAGAAGAACAGTTGTGGGATAAATTTGTGCt > 1:3234049/1‑69 (MQ=255) ccccAATACTATTGTGAAAGATCCGCAACATGTCGAGACGGAAGAACAGtt < 1:2807411/51‑1 (MQ=255) ccccAATACTATTGTGAAAGATCCGCAACATGTCGAGACGGAAGAACAGtt < 1:933873/51‑1 (MQ=255) cTATTGTGAAAGATCCGGCGCATGTCGAGACAGAAGAACAGTTGTGGGATa > 1:2680642/1‑51 (MQ=255) cTATTGTGAAAGATCCGGCGCATGTCGAGACAGAAGAACAGTTGTGGGATAAATTTGTGCTGATGcacc > 1:403347/1‑69 (MQ=255) cTATTGTGAAAGATCCGGCGCATGTCGAGACAGAAGAACAGTTGTGGGATAAATTTGTGCTGATGcacc > 1:2807220/1‑69 (MQ=255) cTATTGTGAAAGATCCGGCGCATGTCGAGACAGAAGAACAGTTGTGGGATAAATTTGTGCTGATGcacc > 1:1032801/1‑69 (MQ=255) cTATTGTGAAAGATCCGGCGCATGTCGAGACAGAAGAACAGTTGTGGGATAAATTTGTGCTGATGcacc > 1:1344067/1‑69 (MQ=255) cTATTGTGAAAGATCCGGCGCATGTCGAGACAGAAGAACAGTTGTGGGATAAATTTGTGCTGATGcacc > 1:1446156/1‑69 (MQ=255) cTATTGTGAAAGATCCGGCGCATGTCGAGACAGAAGAACAGTTGTGGGATAAATTTGTGCTGATGcacc > 1:1571012/1‑69 (MQ=255) cTATTGTGAAAGATCCGGCGCATGTCGAGACAGAAGAACAGTTGTGGGATAAATTTGTGCTGATGcacc > 1:1192462/1‑69 (MQ=255) cTATTGTGAAAGATCCGGCGCATGTCGAGACAGAAGAACAGTTGTGGGATAAATTTGTGCTGATGcacc > 1:1887400/1‑69 (MQ=255) cTATTGTGAAAGATCCGGCGCATGTCGAGACAGAAGAACAGTTGTGGGATAAATTTGTGCTGATGcac > 1:2206174/1‑68 (MQ=255) gtgAAAGATCCGGCGCATGTCGAGACAGAAGAACAGTTGTGGGATAAATTTGTGCTGATGCACCAcgcg > 1:885371/1‑69 (MQ=255) tCCGGCGCATGTCGAGACAGAAGAACAGTTGTGGGATAAATTTGTGCTGATGCACCACGCGGATaaaa > 1:644290/1‑68 (MQ=255) ccGGCGCATGTCGAGACAGAAGAACAGTTGTGGGATAAATTTGTGCTGATGCACCACGCGGATaaaa > 1:2510821/1‑67 (MQ=255) ccGGCGCATGTCGAGACAGAAGAACAGTTGTGGGATAAATTTGTGCTGATGCACCACGCGGATaaaa > 1:1276818/1‑67 (MQ=255) ccGGCGCATGTCGAGACAGAAGAACAGTTGTGGGATAAATTTGTGCTGATGCACCACGCGGATAAAAt > 1:2526018/1‑68 (MQ=255) ccGGCGCATGTCGAGACAGAAGAACAGTTGTGGGATAAATTTGTGCTGATGCACCACGCGGATAAAAt > 1:3159488/1‑68 (MQ=255) ccGGCGCATGTCGAGACAGAAGAACAGTTGTGGGATAAATTTGTGCTGATGCACCACGCGGATAAAAt > 1:451710/1‑68 (MQ=255) ccGGCGCATGTCGAGACAGAAGAACAGTTGTGGGATAAATTTGTGCTGATGCACCACGCGGATAAAAt > 1:576064/1‑68 (MQ=255) ccGGCGCATGTCGAGACAGAAGAACAGTTGTGGGATAAATTTGTGCTGATGCACCACGCGGATAAAAt > 1:44814/1‑68 (MQ=255) ccGGCGCATGTCGAGACAGAAGAACAGTTGTGGGATAAATTTGTGCTGATGCACCACGCGGATAAAAt > 1:1281035/1‑68 (MQ=255) acATGTCGAGACGGGAGAACAGTTGTGGGATAAAtt > 1:1164294/2‑36 (MQ=37) acATGTCGAGACGGAAGAACAGTTGTGGGATAAAtt > 1:3208065/2‑36 (MQ=38) acATGTCGAGACGGAAGAACAGTTGTGGGATAAAtt > 1:321777/2‑36 (MQ=38) acATGTCGAGACGGAAGAACAGTTGTGGGATAAAtt > 1:407826/2‑36 (MQ=38) acATGTCGAGACGGAAGAACAGTTGTGGGATAAAtt > 1:951033/2‑36 (MQ=38) acATGTCGAGACGGAAGAACAGTTGTGGGATAAAtt > 1:3248577/2‑36 (MQ=38) acATGTCGAGACGGAAGAACAGTTGTGGGATAAAtt > 1:643476/2‑36 (MQ=38) acATGTCGAGACGGAAGAACAGTTGTGGGATAAAtt > 1:3375558/2‑36 (MQ=38) acATGTCGAGACGGAAGAACAGTTGTGGGATAAAtt > 1:522922/2‑36 (MQ=38) acATGTCGAGACGGAAGAACAGTTGTGGGATAAAtt > 1:3421358/2‑36 (MQ=38) acATGTCGAGACGGAAGAACAGTTGTGGGATAAAtt > 1:451966/2‑36 (MQ=38) acATGTCGAGACGGAAGAACAGTTGTGGGATAAAtt > 1:432104/2‑36 (MQ=38) acATGTCGAGACGGAAGAACAGTTGTGGGATAAAtt > 1:3008593/2‑36 (MQ=38) acATGTCGAGACGGAAGAACAGTTGTGGGATAAAtt > 1:1087049/2‑36 (MQ=38) acATGTCGAGACGGAAGAACAGTTGTGGGATAAAtt > 1:1143772/2‑36 (MQ=38) acATGTCGAGACGGAAGAACAGTTGTGGGATAAAtt > 1:1158759/2‑36 (MQ=38) acATGTCGAGACGGAAGAACAGTTGTGGGATAAAtt > 1:1276077/2‑36 (MQ=38) acATGTCGAGACGGAAGAACAGTTGTGGGATAAAtt > 1:1291762/2‑36 (MQ=38) acATGTCGAGACGGAAGAACAGTTGTGGGATAAAtt > 1:1499888/2‑36 (MQ=38) acATGTCGAGACGGAAGAACAGTTGTGGGATAAAtt > 1:1523406/2‑36 (MQ=38) acATGTCGAGACGGAAGAACAGTTGTGGGATAAAtt > 1:1696093/2‑36 (MQ=38) acATGTCGAGACGGAAGAACAGTTGTGGGATAAAtt > 1:2100910/2‑36 (MQ=38) acATGTCGAGACGGAAGAACAGTTGTGGGATAAAtt > 1:2451537/2‑36 (MQ=38) acATGTCGAGACGGAAGAACAGTTGTGGGATAAAtt > 1:2515433/2‑36 (MQ=38) acATGTCGAGACGGAAGAACAGTTGTGGGATAAAtt > 1:3006539/2‑36 (MQ=38) acATGTCGAGACGGAAGAACAGTTGTGGGATAAAtt > 1:2918443/2‑36 (MQ=38) acATGTCGAGACGGAAGAACAGTTGTGGGATAAAtt > 1:2598753/2‑36 (MQ=38) acATGTCGAGACGGAAGAACAGTTGTGGGATAAAtt > 1:2590735/2‑36 (MQ=38) acATGTCGAGACGGAAGAACAGTTGTGGGATAAAtt > 1:2588659/2‑36 (MQ=38) acATGTCGAGACGGAAGAACAGTTGTGGGATAAAtt > 1:2551505/2‑36 (MQ=38) tCGAGACAGAAGAACAGTTGTGGGATAAATTTGTGCTGATGCACCACGCGGATAAAATTTAATATTCTa > 1:1760908/1‑69 (MQ=255) tCGAGACAGAAGAACAGTTGTGGGATAAATTTGTGCTGATGCACCACGCGGATAAAATTTAATATTCTa < 1:2018145/69‑1 (MQ=255) gagaCAGAAGAACAGTTGTGGGATAAATTTGTGCTGATGCACCACGCGGATAAAATTTaa > 1:1797187/1‑60 (MQ=255) gagaCAGAAGAACAGTTGTGGGATAAATTTGTGCTGATGCACCACGCGGATAAAATTTaa > 1:1738903/1‑60 (MQ=255) gagaCAGAAGAACAGTTGTGGGATAAATTTGTGCTGATGCACCACGCGGATAAAATTTaa > 1:19742/1‑60 (MQ=255) gagaCAGAAGAACAGTTGTGGGATAAATTTGTGCTGATGCACCACGCGGATAAAATTTaa > 1:2845749/1‑60 (MQ=255) gagaCAGAAGAACAGTTGTGGGATAAATTTGTGCTGATGCACCACGCGGATAAAATTTaa > 1:2973244/1‑60 (MQ=255) gagaCAGAAGAACAGTTGTGGGATAAATTTGTGCTGATGCACCACGCGGATAAAATTTaa > 1:355922/1‑60 (MQ=255) gagaCAGAAGAACAGTTGTGGGATAAATTTCTGCTGAAGCACCACGCGGAt > 1:1914426/1‑51 (MQ=255) agaagaACAGTTGTGGGATAAATTTGTGCTGATGCACCACGCGGATAAAAttt > 1:1213495/1‑53 (MQ=255) agaagaACAGTTGTGGGATAAATTTGTGCTGATGCACCACGCGGATAAAAttt > 1:3045208/1‑53 (MQ=255) | AACCTGCCAGAAGGTGTGCCCGATCCCCAATACTATTGTGAAAGATCCGGCGCATGTCGAGACAGAAGAACAGTTGTGGGATAAATTTGTGCTGATGCACCACGCGGATAAAATTTAATATTCTA > minE/1732492‑1732616 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |