| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,733,340 | C→G | 51.4% | R115R (CGG→CGC) | acpS ← | holo‑[acyl‑carrier‑protein] synthase 1 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,733,340 | 0 | C | G | 51.4% | 4.7 / 128.8 | 105 | R115R (CGG→CGC) | acpS | holo‑[acyl‑carrier‑protein] synthase 1 |
| Reads supporting (aligned to +/- strand): ref base C (4/47); new base G (16/38); total (20/85) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.75e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.42e-01 | |||||||||||
Only 100 of 105 total aligned reads displayed.
CTTTTTAAAGAGAACGGTATTATTTTTAACTTTCAATAATTACCGTGGCACAAGCATAGTGCCGCTCATCTGCCAGCGTTACATGCATATTTGCAACGCCCAGCTTTTCCGCCAGTTTT > minE/1733279‑1733397 | cTTTTTAAAGAGAACGGTATTATTTTTAACTTTCAATAATTACCGTGGCACAAGCATAGTGCCGCTCa < 1:383168/68‑1 (MQ=255) aaCGGTATTATTTTTAACTTTCAATAATTACCGTGGCACAAGCATAGTGCCGCTCATCTGCCAGCGTTa < 1:2739574/69‑1 (MQ=255) aaCGGTATTATTTTTAACTTTCAATAATTACCGTGGCACAAGCATAGTGCCGCTCATCTGCCAGCGTTa < 1:183141/69‑1 (MQ=255) aaCGGTATTATTTTTAACTTTCAATAATTACCGTGGCACAAGCATAGTGCCGCTCATCTGCCAGCGTTa < 1:3077747/69‑1 (MQ=255) aaCGGTATTATTTTTAACTTTCAATAATTACCGTGGCACAAGCATAGTGCCGCTCATCTGCCAGCGTTa < 1:3375610/69‑1 (MQ=255) aaCGGTATTATTTTTAACTTTCAATAATTACCGTGGCACAAGCATAGTGCCGCTCATCTGCCAGCGTTa < 1:3392354/69‑1 (MQ=255) aaCGGTATTATTTTTAACTTTCAATAATTACCGTGGCACAAGCATAGTGCCGCTCATCTGCCAGCGTTa < 1:1836856/69‑1 (MQ=255) aaCGGTATTATTTTTAACTTTCAATAATTACCGTGGCACAAGCATAGTGCCGCTCATCTGCCAGCGTTa < 1:186581/69‑1 (MQ=255) aaCGGTATTATTTTTAACTTTCAATAATTACCGTGGCACAAGCATAGTGCCGCTCATCTGCCAGCGTTa < 1:581150/69‑1 (MQ=255) aaCGGTATTATTTTTAACTTTCAATAATTACCGTGGCACAAGCATAGTGCCGCTCATCTGCCAGCGTTa < 1:2502330/69‑1 (MQ=255) aaCGGTATTATTTTTAACTTTCAATAATTACCGTGGCACAAGCATAGTGCCGCTCATCTGCCAGCGTTa < 1:955122/69‑1 (MQ=255) aaCGGTATTATTTTTAACTTTCAATAATTACCGTGGCACAAGCATAGTGCCGCTCATCTGCCAGCGTTa < 1:2503015/69‑1 (MQ=255) aaCGGTATTATTTTTAACTTTCAATAATTACCGTGGCACAAGCATAGTGCCGCTCATCTGCCAGCGTTa < 1:1914485/69‑1 (MQ=255) tttCAATAATTACCGTGGCACAAGCATAGTGGCGCTCATCGGCAAGCGTTACATGCATAtttac > 1:2564580/1‑62 (MQ=255) tttCAATAATTACCGTGGCACAAGCATAGTGGCGCTCATCGGCAAGCGTTACATGCATAtttac > 1:1935375/1‑62 (MQ=255) tttCAATAATTACCGTGGCACAAGCATAGTGGCGCTCATCGGCAAGCGTTACATGCATAtttac > 1:1770605/1‑62 (MQ=255) tttCAATAATTACCGTGGCACAAGCATAGTGGCGCTCATCGGCAAGCGTTACATGCATAtttac > 1:1959882/1‑62 (MQ=255) tttCAATAATTACCGTGGCACAAGCATAGTGGCGCTCATCGGCAAGCGTTACATGCATAtttac > 1:2414742/1‑62 (MQ=255) tttCAATAATTACCGTGGCACAAGCATAGTGGCGCTCATCGGCAAGCGTTACATGCATAtttac > 1:2034138/1‑62 (MQ=255) tttCAATAATTACCGTGGCACAAGCATAGTGGCGCTCATCGGCAAGCGTTACATGCATAtttac > 1:2409116/1‑62 (MQ=255) tttCAATAATTACCGTGGCACAAGCATAGTGGCGCTCATCGGCAAGCGTTACATGCATAtttac > 1:2803347/1‑62 (MQ=255) tttCAATAATTACCGTGGCACAAGCATAGTGGCGCTCATCGGCAAGCGTTACATGCATAtttac > 1:299627/1‑62 (MQ=255) tttCAATAATTACCGTGGCACAAGCATAGTGGCGCTCATCGGCAAGCGTTACATGCATAtttac > 1:3000148/1‑62 (MQ=255) tttCAATAATTACCGTGGCACAAGCATAGTGGCGCTCATCGGCAAGCGTTACATGCATAtttac > 1:519981/1‑62 (MQ=255) tttCAATAATTACCGTGGCACAAGCATAGTGGCGCTCATCGGCAAGCGTTACATGCATAtttac > 1:652451/1‑62 (MQ=255) tttCAATAATTACCGTGGCACAAGCATAGTGGCGCTCATCGGCAAGCGTTACATGCATAtttac > 1:2343344/1‑62 (MQ=255) tttCAATAATTACCGTGGCACAAGCATAGTGGCGCTCATCGGCAAGCGTTACATGCATAttta > 1:227768/1‑62 (MQ=255) tttCAATAATTACCGTGGCACAAGCATAGTGGCGCTCATCGGCAAGCGTTACATGCATAttt > 1:2123585/1‑62 (MQ=255) attCAATAATTACCGTGGCACAAGCAAAGTGGCGCTCATc > 1:485691/2‑40 (MQ=38) cAATAATTACCGTGGCACAAGCATAGTGGCGCTCATCGGCAAGCGtt < 1:2637476/47‑1 (MQ=255) cAATAATTACCGTGGCACAAGCATAGTGGCGCTCATCGGCAAGCGtt < 1:2527485/47‑1 (MQ=255) cAATAATTACCGTGGCACAAGCATAGTGGCGCTCATCGGCAAGCGtt < 1:2589171/47‑1 (MQ=255) cAATAATTACCGTGGCACAAGCATAGTGGCGCTCATCGGCAAGCGtt < 1:2599704/47‑1 (MQ=255) cAATAATTACCGTGGCACAAGCATAGTGGCGCTCATCGGCAAGCGtt < 1:992170/47‑1 (MQ=255) cAATAATTACCGTGGCACAAGCATAGTGGCGCTCATCGGCAAGCGtt < 1:2765217/47‑1 (MQ=255) cAATAATTACCGTGGCACAAGCATAGTGGCGCTCATCGGCAAGCGtt < 1:2947157/47‑1 (MQ=255) cAATAATTACCGTGGCACAAGCATAGTGGCGCTCATCGGCAAGCGtt < 1:432670/47‑1 (MQ=255) cAATAATTACCGTGGCACAAGCATAGTGGCGCTCATCGGCAAGCGtt < 1:955704/47‑1 (MQ=255) cAATAATTACCGTGGCACAAGCATAGTGGCGCTCATCGGCAAGCGtt < 1:104413/47‑1 (MQ=255) cAATAATTACCGTGGCACAAGCATAGTGGCGCTCATCGGCAAGCGtt < 1:1516356/47‑1 (MQ=255) cAATAATTACCGTGGCACAAGCATAGTGGCGCTCATCGGCAAGCGtt < 1:1919381/47‑1 (MQ=255) cAATAATTACCGTGGCACAAGCATAGTGGCGCTCATCGGCAAGCGtt < 1:1914264/47‑1 (MQ=255) cAATAATTACCGTGGCACAAGCATAGTGGCGCTCATCGGCAAGCGtt < 1:1429188/47‑1 (MQ=255) cAATAATTACCGTGGCACAAGCATAGTGGCGCTCATCGGCAAGCGtt < 1:127581/47‑1 (MQ=255) cAATAATTACCGTGGCACAAGCATAGTGGCGCTCATCGGCAAGCGtt < 1:1423180/47‑1 (MQ=255) cAATAATTACCGTGGCACAAGCATAGTGGCGCTCATCGGCAAGCGtt < 1:1405053/47‑1 (MQ=255) atTACCGTGGCACAAGCATAGTGCCGCTCATCTGCCAGCGTTACATGCATATTTGCAACGCCCATCttt < 1:1334074/69‑1 (MQ=255) atTACCGTGGCACAAGCATAGTGCCGCTCATCTGCCAGCGTTACATGCATATTTGCAACGCCCAGCttt > 1:1775263/1‑69 (MQ=255) atTACCGTGGCACAAGCATAGTGCCGCTCATCTGCCAGCGTTACATGCATATTTGCAACGCCCAGCttt < 1:2960117/69‑1 (MQ=255) atTACCGTGGCACAAGCATAGTGCCGCTCATCTGCCAGCGTTACATGCATATTTGCAACGCCCAGCttt < 1:3001328/69‑1 (MQ=255) atTACCGTGGCACAAGCATAGTGCCGCTCATCTGCCAGCGTTACATGCATATTTGCAACGCCCAGCttt < 1:1556746/69‑1 (MQ=255) atTACCGTGGCACAAGCATAGTGCCGCTCATCTGCCAGCGTTACATGCATATTTGCAACGCCCAGCttt > 1:767341/1‑69 (MQ=255) atTACCGTGGCACAAGCATAGTGCCGCTCATCTGCCAGCGTTACATGCATATTTGCAACGCCCAGCttt > 1:3362929/1‑69 (MQ=255) atTACCGTGGCACAAGCATAGTGCCGCTCATCTGCCAGCGTTACATGCATATTTGCAACGCCCAGCttt < 1:2529774/69‑1 (MQ=255) atTACCGTGGCACAAGCATAGTGCCGCTCATCTGCCAGCGTTACATGCATATTTGCAACGCCCAGCttt > 1:1974119/1‑69 (MQ=255) tACCGTGGCACAAGCATAGTGGCGCTCATCGGCAAGCGtt < 1:1295937/40‑1 (MQ=255) aCCGTGGCACAAGCATAGTGGCGCTCATCGGCAAGCGTTACATGCATAtt < 1:2228361/50‑1 (MQ=255) aCCGTGGCACAAGCATAGTGGCGCTCATCGGCAAGCGTTACATGCATAtt < 1:135822/50‑1 (MQ=255) aCCGTGGCACAAGCATAGTGGCGCTCATCGGCAAGCGTTACATGCATAtt < 1:3112681/50‑1 (MQ=255) aCCGTGGCACAAGCATAGTGGCGCTCATCGGCAAGCGTTACATGCATAtt < 1:2190403/50‑1 (MQ=255) aCCGTGGCACAAGCATAGTGGCGCTCATCGGCAAGCGTTACATGCATAtt < 1:483272/50‑1 (MQ=255) aCCGTGGCACAAGCATAGTGGCGCTCATCGGCAAGCGTTACATGCATAtt < 1:512300/50‑1 (MQ=255) aCCGTGGCACAAGCATAGTGGCGCTCATCGGCAAGCGTTACATGCATAtt < 1:1512523/50‑1 (MQ=255) aCCGTGGCACAAGCATAGTGGCGCTCATCGGCAAGCGTTACATGCATAtt < 1:61447/50‑1 (MQ=255) aCCGTGGCACAAGCATAGTGGCGCTCATCGGCAAGCGTTACATGCATAtt < 1:797786/50‑1 (MQ=255) aCCGTGGCACAAGCATAGTGGCGCTCATCGGCAAGCGTTACATGCATAtt < 1:2168846/50‑1 (MQ=255) aCCGTGGCACAAGCATAGTGGCGCTCATCGGCAAGCGTTACATGCATAtt < 1:2852366/50‑1 (MQ=255) aCCGTGGCACAAGCATAGTGGCGCTCATCGGCAAGCGTTACATGCATAtt < 1:2801132/50‑1 (MQ=255) aCCGTGGCACAAGCATAGTGGCGCTCATCGGCAAGCGTTACATGCATAtt < 1:278762/50‑1 (MQ=255) aCCGTGGCACAAGCATAGTGGCGCTCATCGGCAAGCGTTACATGCATAtt < 1:1561228/50‑1 (MQ=255) aCCGTGGCACAAGCATAGTGGCGCTCATCGGCAAGCGTTACATGCATAtt < 1:2443157/50‑1 (MQ=255) aCCGTGGCACAAGCATAGTGGCGCTCATCGGCAAGCGTTACATGCATAtt < 1:2666711/50‑1 (MQ=255) cacaAGCATAGTGGCGCTCATCGGCAAGCGTTACATGCATAtt < 1:1682455/43‑1 (MQ=255) caAGCATAGTGGCGCTCATCGGCAAGCGTTACATGCATAtt < 1:69694/41‑1 (MQ=255) caAGCATAGTGCCGCTCATCTGCCAGCGTTACATGCATATTTGCAACGCGCAGCTTTTCCGCCAGtttt < 1:2184818/69‑1 (MQ=255) caAGCATAGTGCCGCTCATCTGCCAGCGTTACATGCATATTTGCAACGCCCAGCTTTTCCGCCAGtttt < 1:915246/69‑1 (MQ=255) caAGCATAGTGCCGCTCATCTGCCAGCGTTACATGCATATTTGCAACGCCCAGCTTTTCCGCCAGtttt < 1:78544/69‑1 (MQ=255) caAGCATAGTGCCGCTCATCTGCCAGCGTTACATGCATATTTGCAACGCCCAGCTTTTCCGCCAGtttt < 1:948485/69‑1 (MQ=255) caAGCATAGTGCCGCTCATCTGCCAGCGTTACATGCATATTTGCAACGCCCAGCTTTTCCGCCAGtttt < 1:1151644/69‑1 (MQ=255) caAGCATAGTGCCGCTCATCTGCCAGCGTTACATGCATATTTGCAACGCCCAGCTTTTCCGCCAGtttt < 1:722924/69‑1 (MQ=255) caAGCATAGTGCCGCTCATCTGCCAGCGTTACATGCATATTTGCAACGCCCAGCTTTTCCGCCAGtttt < 1:987817/69‑1 (MQ=255) caAGCATAGTGCCGCTCATCTGCCAGCGTTACATGCATATTTGCAACGCCCAGCTTTTCCGCCAGtttt < 1:891180/69‑1 (MQ=255) caAGCATAGTGCCGCTCATCTGCCAGCGTTACATGCATATTTGCAACGCCCAGCTTTTCCGCCAGtttt < 1:65053/69‑1 (MQ=255) caAGCATAGTGCCGCTCATCTGCCAGCGTTACATGCATATTTGCAACGCCCAGCTTTTCCGCCAGtttt < 1:3086536/69‑1 (MQ=255) caAGCATAGTGCCGCTCATCTGCCAGCGTTACATGCATATTTGCAACGCCCAGCTTTTCCGCCAGtttt < 1:130094/69‑1 (MQ=255) caAGCATAGTGCCGCTCATCTGCCAGCGTTACATGCATATTTGCAACGCCCAGCTTTTCCGCCAGtttt < 1:3358938/69‑1 (MQ=255) caAGCATAGTGCCGCTCATCTGCCAGCGTTACATGCATATTTGCAACGCCCAGCTTTTCCGCCAGtttt < 1:1503313/69‑1 (MQ=255) caAGCATAGTGCCGCTCATCTGCCAGCGTTACATGCATATTTGCAACGCCCAGCTTTTCCGCCAGtttt < 1:289454/69‑1 (MQ=255) caAGCATAGTGCCGCTCATCTGCCAGCGTTACATGCATATTTGCAACGCCCAGCTTTTCCGCCAGtttt < 1:1882134/69‑1 (MQ=255) caAGCATAGTGCCGCTCATCTGCCAGCGTTACATGCATATTTGCAACGCCCAGCTTTTCCGCCAGtttt < 1:2012848/69‑1 (MQ=255) caAGCATAGTGCCGCTCATCTGCCAGCGTTACATGCATATTTGCAACGCCCAGCTTTTACGCCAGtttt < 1:2938797/69‑1 (MQ=255) caAGCATAGTGCCGCTCATCAGCCAGCGTTACATGCATATTTGCAACGCCCAGCTTTTCCGCCAGtttt < 1:1909314/69‑1 (MQ=255) aaGCATAGTGCCGCTCATCTGCCAGCGTTACATGCATATTTGCAACGCCCAGCTTTTCCGCCAGtttt < 1:602728/68‑1 (MQ=255) aaGCATAGTGCCGCTCATCTGCCAGCGTTACATGCATATTTGCAACGCCCAGCTTTTCCGCCAGtttt < 1:360456/68‑1 (MQ=255) gTGCCGCTCATCTGCCAGCGTTACATGCATATTTGCAACGCCCAGCTTTTCCGCCAGtttt < 1:1307764/61‑1 (MQ=255) gTGCCGCTCATCTGCCAGCGTTACATGCATATTTGCAACGCCCAGCTTTTCCGCCAGtttt < 1:1521286/61‑1 (MQ=255) gTGCCGCTCATCTGCCAGCGTTACATGCATATTTGCAACGCCCAGCTTTTCCGCCAGtttt < 1:834499/61‑1 (MQ=255) gTGCCGCTCATCTGCCAGCGTTACATGCATATTTGCAACGCCCAGCTTTTCCGCCAGtttt < 1:1598005/61‑1 (MQ=255) gTGCCGCTCATCTGCCAGCGTTACATGCATATTTGCAACGCCCAGCTTTTCCGCCAGtttt < 1:1734824/61‑1 (MQ=255) gTGCCGCTCATCTGCCAGCGTTACATGCATATTTGCAACGCCCAGCTTTTCCGCCAGtttt < 1:1126589/61‑1 (MQ=255) | CTTTTTAAAGAGAACGGTATTATTTTTAACTTTCAATAATTACCGTGGCACAAGCATAGTGCCGCTCATCTGCCAGCGTTACATGCATATTTGCAACGCCCAGCTTTTCCGCCAGTTTT > minE/1733279‑1733397 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |