Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,737,138 G→A 100% G286G (GGC→GGT lepB ← leader peptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,737,1380GA76.3% 19.7 / 2.7 21G286G (GGC→GGTlepBleader peptidase
Reads supporting (aligned to +/- strand):  ref base G (4/1);  new base A (16/0);  total (20/1)
Fisher's exact test for biased strand distribution p-value = 2.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.75e-01
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CAGATAGCCGTTGCCCGACCGACCAGATTCGCTTCCGGCACAAAGCCCCAGTAACGGCTGTCCGCGCTGTTGTCGCGGTTGTCGCCCATCATGAAGTATTGT  >  minE/1737094‑1737195
                                            |                                                         
cAGATAGCCGTTGCCCGACCGACCAGATTCGCTTCCGGCACAAAGCCCCAGTAACGGCTGTCCGCGCtg                                   >  1:3389693/1‑69 (MQ=255)
    tAGCCGTTGCCCGACCGACCAGATTCGCTTCCGGCACAAAGCCCCAGTAACGGCTGTCCGCGCtgttgt                               >  1:3140436/1‑69 (MQ=255)
            gcccgcccGACCAGATTCGCTTCCGGCACAAAGCCCCAGTAACGGCTGTCCGCGCTGTTGTCGCGGTTg                       >  1:181248/1‑69 (MQ=255)
            gCCCGACCGACCAGATTCGCTTCCGGCACAAAGCCCCAGTAACGGCTGTCCGCGCTGTTGTCGCGGTTg                       >  1:1124685/1‑69 (MQ=255)
                      ccAGATTCGCTTTAGGCACAAAACACCAGTAACGGCTGTCCGCGCTGTTGTCGCGGTTGTCg                    >  1:2793220/1‑62 (MQ=255)
                      ccAGATTCGCTTCAGGCACAAAACCCCAGTAACGGCTGTCCGCGCTGTTGTCGCGGTTGTCg                    >  1:838184/1‑62 (MQ=255)
                      ccAGATTCGCTTCAGGCACAAAACCCCAGTAACGGCTGTCCGCGCTGTTGTCGCGGTTGTCg                    >  1:1052428/1‑62 (MQ=255)
                      ccAGATTCGCTTCAGGCACAAAACCCCAGTAACGGCTGTCCGCGCTGTTGTCGCGGTTGTCg                    >  1:534772/1‑62 (MQ=255)
                      ccAGATTCGCTTCAGGCACAAAACCCCAGTAACGGCTGTCCGCGCTGTTGTCGCGGTTGTCg                    >  1:274114/1‑62 (MQ=255)
                      ccAGATTCGCTTCAGGCACAAAACCCCAGTAACGGCTGTCCGCGCTGTTGTCGCGGTTGTCg                    >  1:2373427/1‑62 (MQ=255)
                      ccAGATTCGCTTCAGGCACAAAACCCCAGTAACGGCTGTCCGCGCTGTTGTCGCGGTTGTCg                    >  1:2267918/1‑62 (MQ=255)
                      ccAGATTCGCTTCAGGCACAAAACCCCAGTAACGGCTGTCCGCGCTGTTGTCGCGGTTGTCg                    >  1:2129589/1‑62 (MQ=255)
                      ccAGATTCGCTTCAGGCACAAAACCCCAGTAACGGCTGTCCGCGCTGTTGTCGCGGTTGTCg                    >  1:2125530/1‑62 (MQ=255)
                      ccAGATTCGCTTCAGGCACAAAACCCCAGTAACGGCTGTCCGCGCTGTTGTCGCGGTTGTCg                    >  1:183531/1‑62 (MQ=255)
                      ccAGATTCGCTTCAGGCACAAAACCCCAGTAACGGCTGTCCGCGCTGTTGTCGCGGTTGTCg                    >  1:1818012/1‑62 (MQ=255)
                      ccAGATTCGCTTCAGGCACAAAACCCCAGTAACGGCTGTCCGCGCTGTTGTCGCGGTTGTCg                    >  1:1478871/1‑62 (MQ=255)
                      ccAGATTCGCTTCAGGCACAAAACCCCAGTAACGGCTGTCCGCGCTGTTGTCGCGGTTGTCg                    >  1:147709/1‑62 (MQ=255)
                      ccAGATTCGCTTCAGGCACAAAACCCCAGTAACGGCTGTCCGCGCTGTTGTCGCGGTTGTCg                    >  1:137361/1‑62 (MQ=255)
                      ccAGATTCGCTTCAGGCACAAAACCCCAGTAACGGCTGTCCGCGCTGTTGTCGCGGTTGTCg                    >  1:1329756/1‑62 (MQ=255)
                      ccAGATTCGCTTCAGGCACAAAACCCCAGTAACGGCTGTCCGCGCTGTTGTCGCGGTTGTCg                    >  1:1137245/1‑62 (MQ=255)
                                 tCCGGCACAAAGCCCCAGTAACGGCTGTCCGCGCTGTTGTCGCGGTTGTCGCCCATCATGAAGTATTGt  <  1:1727573/69‑1 (MQ=255)
                                            |                                                         
CAGATAGCCGTTGCCCGACCGACCAGATTCGCTTCCGGCACAAAGCCCCAGTAACGGCTGTCCGCGCTGTTGTCGCGGTTGTCGCCCATCATGAAGTATTGT  >  minE/1737094‑1737195

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: