| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,737,138 | G→A | 100% | G286G (GGC→GGT) | lepB ← | leader peptidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,737,138 | 0 | G | A | 76.3% | 19.7 / 2.7 | 21 | G286G (GGC→GGT) | lepB | leader peptidase |
| Reads supporting (aligned to +/- strand): ref base G (4/1); new base A (16/0); total (20/1) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.38e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.75e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CAGATAGCCGTTGCCCGACCGACCAGATTCGCTTCCGGCACAAAGCCCCAGTAACGGCTGTCCGCGCTGTTGTCGCGGTTGTCGCCCATCATGAAGTATTGT > minE/1737094‑1737195 | cAGATAGCCGTTGCCCGACCGACCAGATTCGCTTCCGGCACAAAGCCCCAGTAACGGCTGTCCGCGCtg > 1:3389693/1‑69 (MQ=255) tAGCCGTTGCCCGACCGACCAGATTCGCTTCCGGCACAAAGCCCCAGTAACGGCTGTCCGCGCtgttgt > 1:3140436/1‑69 (MQ=255) gcccgcccGACCAGATTCGCTTCCGGCACAAAGCCCCAGTAACGGCTGTCCGCGCTGTTGTCGCGGTTg > 1:181248/1‑69 (MQ=255) gCCCGACCGACCAGATTCGCTTCCGGCACAAAGCCCCAGTAACGGCTGTCCGCGCTGTTGTCGCGGTTg > 1:1124685/1‑69 (MQ=255) ccAGATTCGCTTTAGGCACAAAACACCAGTAACGGCTGTCCGCGCTGTTGTCGCGGTTGTCg > 1:2793220/1‑62 (MQ=255) ccAGATTCGCTTCAGGCACAAAACCCCAGTAACGGCTGTCCGCGCTGTTGTCGCGGTTGTCg > 1:838184/1‑62 (MQ=255) ccAGATTCGCTTCAGGCACAAAACCCCAGTAACGGCTGTCCGCGCTGTTGTCGCGGTTGTCg > 1:1052428/1‑62 (MQ=255) ccAGATTCGCTTCAGGCACAAAACCCCAGTAACGGCTGTCCGCGCTGTTGTCGCGGTTGTCg > 1:534772/1‑62 (MQ=255) ccAGATTCGCTTCAGGCACAAAACCCCAGTAACGGCTGTCCGCGCTGTTGTCGCGGTTGTCg > 1:274114/1‑62 (MQ=255) ccAGATTCGCTTCAGGCACAAAACCCCAGTAACGGCTGTCCGCGCTGTTGTCGCGGTTGTCg > 1:2373427/1‑62 (MQ=255) ccAGATTCGCTTCAGGCACAAAACCCCAGTAACGGCTGTCCGCGCTGTTGTCGCGGTTGTCg > 1:2267918/1‑62 (MQ=255) ccAGATTCGCTTCAGGCACAAAACCCCAGTAACGGCTGTCCGCGCTGTTGTCGCGGTTGTCg > 1:2129589/1‑62 (MQ=255) ccAGATTCGCTTCAGGCACAAAACCCCAGTAACGGCTGTCCGCGCTGTTGTCGCGGTTGTCg > 1:2125530/1‑62 (MQ=255) ccAGATTCGCTTCAGGCACAAAACCCCAGTAACGGCTGTCCGCGCTGTTGTCGCGGTTGTCg > 1:183531/1‑62 (MQ=255) ccAGATTCGCTTCAGGCACAAAACCCCAGTAACGGCTGTCCGCGCTGTTGTCGCGGTTGTCg > 1:1818012/1‑62 (MQ=255) ccAGATTCGCTTCAGGCACAAAACCCCAGTAACGGCTGTCCGCGCTGTTGTCGCGGTTGTCg > 1:1478871/1‑62 (MQ=255) ccAGATTCGCTTCAGGCACAAAACCCCAGTAACGGCTGTCCGCGCTGTTGTCGCGGTTGTCg > 1:147709/1‑62 (MQ=255) ccAGATTCGCTTCAGGCACAAAACCCCAGTAACGGCTGTCCGCGCTGTTGTCGCGGTTGTCg > 1:137361/1‑62 (MQ=255) ccAGATTCGCTTCAGGCACAAAACCCCAGTAACGGCTGTCCGCGCTGTTGTCGCGGTTGTCg > 1:1329756/1‑62 (MQ=255) ccAGATTCGCTTCAGGCACAAAACCCCAGTAACGGCTGTCCGCGCTGTTGTCGCGGTTGTCg > 1:1137245/1‑62 (MQ=255) tCCGGCACAAAGCCCCAGTAACGGCTGTCCGCGCTGTTGTCGCGGTTGTCGCCCATCATGAAGTATTGt < 1:1727573/69‑1 (MQ=255) | CAGATAGCCGTTGCCCGACCGACCAGATTCGCTTCCGGCACAAAGCCCCAGTAACGGCTGTCCGCGCTGTTGTCGCGGTTGTCGCCCATCATGAAGTATTGT > minE/1737094‑1737195 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |