| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,738,392 | C→T | 33.2% | K473K (AAG→AAA) | lepA ← | GTP binding membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,738,392 | 0 | C | T | 33.2% | 55.2 / 34.9 | 69 | K473K (AAG→AAA) | lepA | GTP binding membrane protein |
| Reads supporting (aligned to +/- strand): ref base C (38/8); new base T (3/20); total (41/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.84e-08 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.44e-01 | |||||||||||
AGCGCATCAACACGTTCACCGTTGATTAATACGTCTACACGTACCATGTCGGACGCCTGGAAGCGCTTGAAGTTGTAATCCAGAGACGCATAACCACGCGAGGTAGATTTCAGGCGATCGAAGAAGTCGAGCAC > minE/1738327‑1738460 | aGCGCATCAACACGTTCACCGTTGATTAATACGTCTACACGTACCATGTCGGACGCCTGGAAGCGCTTg < 1:3148109/69‑1 (MQ=255)aGCGCATCAACACGTTCACCGTTGATTAATACGTCTACACGTACCATGTCGGACGCCTGGAAGCGCTTg < 1:3409814/69‑1 (MQ=255)aGCGCATCAACACGTTCACCGTTGATTAATACGTCTACACGTACCATGTCGGACGCCTGGAAGCGCTTg < 1:746266/69‑1 (MQ=255)aGCGCATCAACACGTTCACCGTTGATTAATACGTCTACACGTACCATGTCGGACGCCTGGAAGCGCTTg < 1:3018140/69‑1 (MQ=255) cATCAACACGTTCACCGTTGATTAATACGTCTACACGTACCATGTCGGACGCCTGGAAGCGCTTGAAGt > 1:2319424/1‑69 (MQ=255) cATCAACACGTTCACCGTTGATTAATACGTCTACACGTACCATGTCGGACGCCTGGAAGCGCTTGAAGt > 1:2374842/1‑69 (MQ=255) cATCAACACGTTCACCGTTGATTAATACGTCTACACGTACCATGTCGGACGCCTGGAAGCGCTTGAAGt > 1:3143414/1‑69 (MQ=255) cATCAACACGTTCACCGTTGATTAATACGTCTACACGTACCATGTCGGACGCCTGGAAGCGCTTGAAGt > 1:3229680/1‑69 (MQ=255) cATCAACACGTTCACCGTTGATTAATACGTCTACACGTACCATGTCGGACGCCTGGAAGCGCTTGAAGt > 1:2803100/1‑69 (MQ=255) cATCAACACGTTCACCGTTGATTAATACGTCTACACGTACCATGTCGGACGCCTGGAAGCGCTTGAAGt > 1:600140/1‑69 (MQ=255) cATCAACACGTTCACCGTTGATTAATACGTCTACACGTACCATGTCGGACGCCTGGAAGCGCTAGAAGt > 1:3120888/1‑69 (MQ=255) cACCGTTGATTAATACGTCTACACGTACCATGTCGGACGCCTGGAAGCGCTTGGAGTTGTAATCCaga > 1:2916932/1‑68 (MQ=255) cACCGTTGATTAATACGTCTACACGTACCATGTCGGACGCCTGGAAGCGCTTGAAGTTGTAATCCagag > 1:1813169/1‑69 (MQ=255) cACCGTTGATTAATACGTCTACACGTACCATGTCGGACGCCTGGAAGCGCTTGAAGTTGTAATCCagag > 1:2199581/1‑69 (MQ=255) aCCGTTGATTAATACGTCTACACGTACCATGTCGGACGCCTGGAAGCGTTTGAAGTTGTAATCCAgaga > 1:2509221/1‑69 (MQ=255) aCCGTTGATTAATACGTCTACACGTACCATGTCGGACGCCTGGAAGCGTTTGAAGTTGTAATCCAgaga > 1:3255747/1‑69 (MQ=255) aCCGTTGATTAATACGTCTACACGTACCACGTCGGACGCCTGGAAGCGTTTGAAGTTGTAATCCAgaga > 1:1228804/1‑69 (MQ=255) cGTCTACACGTACCATGTCGGCCGCCTGGAAGCGCTTGAAGTTGTAATCCAGAGACGCATAACCAcgcg > 1:3402960/1‑69 (MQ=255) cacGTACCATGTCGGACGCCTGGAAGCGCTTGAAGTTGTAATCCAGAGACGCATAACCACGCGAGGTAg > 1:2007342/1‑69 (MQ=255) cacGTACCATGTCGGACGCCTGGAAGCGCTTGAAGTTGTAATCCAGAGACGCATAACCACGCGAGGTAg > 1:1675402/1‑69 (MQ=255) cacGTACCATGTCGGACGCCTGGAAGCGCTTGAAGTTGTAATCCAGAGACGCATAACCACGCGAGGTAg > 1:3073136/1‑69 (MQ=255) aCCATGTCGGACGCCTGGAATCGTTTGAAGTTGTAATCCAGAGACGCATAACCACGCGAGGTAGATTTc < 1:2629210/69‑1 (MQ=255) aCCATGTCGGACGCCTGGAATCGTTTGAAGTTGTAATCCAGAGACGCAAAACCACGCGAGGTAGATTTc < 1:585329/69‑1 (MQ=255) aCCATGTCGGACGCCTGGAAGCGTTTGAAGTTGTAATCCAGAGACGCATAACCACGCGAGGTAGATTTc < 1:2063199/69‑1 (MQ=255) aCCATGTCGGACGCCTGGAAGCGTTTGAAGTTGTAATCCAGAGACGCATAACCACGCGAGGTAGATTTc < 1:2516946/69‑1 (MQ=255) aCCATGTCGGACGCCTGGAAGCGTTTGAAGTTGTAATCCAGAGACGCATAACCACGCGAGGTAGATTTc < 1:2776598/69‑1 (MQ=255) aCCATGTCGGACGCCTGGAAGCGTTTGAAGTTGTAATCCAGAGACGCATAACCACGCGAGGTAGATTTc < 1:2919761/69‑1 (MQ=255) aCCATGTCGGACGCCTGGAAGCGTTTGAAGTTGTAATCCAGAGACGCATAACCACGCGAGGTAGATTTc < 1:2944213/69‑1 (MQ=255) aCCATGTCGGACGCCTGGAAGCGTTTGAAGTTGTAATCCAGAGACGCATAACCACGCGAGGTAGATTTc < 1:154736/69‑1 (MQ=255) aCCATGTCGGACGCCTGGAAGCGTTTGAAGTTGTAATCCAGAGACGCATAACCACGCGAGGTAGATTTc < 1:1055105/69‑1 (MQ=255) aCCATGTCGGACGCCTGGAAGCGTTTGAAGTTGTAATCCAGAGACGCATAACCACGCGAGGTAGATTTc < 1:881738/69‑1 (MQ=255) aCCATGTCGGACGCCTGGAAGCGTTTGAAGTTGTAATCCAGAGACGCATAACCACGCGAGGTAGATTTc < 1:830954/69‑1 (MQ=255) aCCATGTCGGACGCCTGGAAGCGTTTGAAGTTGTAATCCAGAGACGCATAACCACGCGAGGTAGATTTc < 1:1333298/69‑1 (MQ=255) aCCATGTCGGACGCCTGGAAGCGTTTGAAGTTGTAATCCAGAGACGCATAACCACGCGAGGTAGATTTc < 1:800160/69‑1 (MQ=255) aCCATGTCGGACGCCTGGAAGCGTTTGAAGTTGTAATCCAGAGACGCATAACCACGCGAGGTAGATTTc < 1:731941/69‑1 (MQ=255) aCCATGTCGGACGCCTGGAAGCGTTTGAAGTTGTAATCCAGAGACGCATAACCACGCGAGGTAGATTTc < 1:1389420/69‑1 (MQ=255) aCCATGTCGGACGCCTGGAAGCGTTTGAAGTTGTAATCCAGAGACGCATAACCACGCGAGGTAGATTTc < 1:1658920/69‑1 (MQ=255) aCCATGTCGGACGCCTGGAAGCGTTTGAAGTTGTAATCCAGAGACGCATAACCACGCGAGGTAGATTTc < 1:529117/69‑1 (MQ=255) aCCATGTCGGACGCCTGGAAGCGTTTGAAGTTGTAATCCAGAGACGCATAACCACGCGAGGTAGATTTc < 1:526102/69‑1 (MQ=255) aCCATGTCGGACGCCTGGAAGCGTTTGAAGTTGTAATCCAGAGACGCATAACCACGCGAGGTAGATTTc < 1:1784528/69‑1 (MQ=255) aCCATGTCGGACGCCTGGAAGCGTTTGAAGTTGTAATCCAGAGACGCATAACCACGCGAGGTAGATTTc < 1:178648/69‑1 (MQ=255) tCGGACGCCTGGAAGCGCTTGAAGTTGTAATCCAGAGACGCATAACCACGCGAGGTAGATTTCAGGCGa > 1:180993/1‑69 (MQ=255) tCGGACGCCTGGAAGCGCTTGAAGTTGTAATCCAGAGACGCATAACCACGCGAGGTAGATTTCAGGCGa > 1:3287570/1‑69 (MQ=255) aCGCCTGGAAGCGCTTGAAGTTGTAATCCAGAGACGCATAACCACGCGAGGTAGATTTCAGGCGAt > 1:366241/1‑66 (MQ=255) aCGCCTGGAAGCGCTTGAAGTTGTAATCCAGAGACGCATAACCACGCGAGGTAGATTTCAGGCGATCg < 1:3197176/68‑1 (MQ=255) aCGCCTGGAAGCGCTTGAAGTTGTAATCCAGAGACGCATAACCACGCGAGGTAGATTTCAGGCGATCg < 1:641384/68‑1 (MQ=255) aCGCCTGGAAGCGCTTGAAGTTGTAATCCAGAGACGCATAACCACGCGAGGTAGATTTCAGGCGATCg < 1:1421934/68‑1 (MQ=255) aCGCCTGGAAGCGCTTGAAGTTGTAATCCAGAGACGCATAACCACGCGAGGTAGATTTCAGGCGATCg < 1:2442223/68‑1 (MQ=255) cGCCTGGAAGCGCTTGAAGTTGTAATCCAGAGACGCATAACCACGCGAGGTAGATTTCAGGCGATCga > 1:3212981/1‑68 (MQ=255) cGCCTGGAAGCGCTTGAAGTTGTAATCCAGAGACGCATAACCACGCGAGGTAGATTTCAGGCGATCga > 1:928303/1‑68 (MQ=255) cGCCTGGAAGCGCTTGAAGTTGTAATCCAGAGACGCATAACCACGCGAGGTAGATTTCAGGCGATCga > 1:3173825/1‑68 (MQ=255) cGCCTGGAAGCGCTTGAAGTTGTAATCCAGAGACGCATAACCACGCGAGGTAGATTTCAGGCGATCga > 1:1009286/1‑68 (MQ=255) cGCCTGGAAGCGCTTGAAGTTGTAATCCAGAGACGCATAACCACGCGAGGTAGATTTCAGGCGATCga > 1:1086184/1‑68 (MQ=255) cGCCTGGAAGCGCTTGAAGTTGTAATCCAGAGACGCATAACCACGCGAGGTAGATTTCAGGCGATCga > 1:1647337/1‑68 (MQ=255) cGCCTGGAAGCGCTTGAAGTTGTAATCCAGAGACGCATAACCACGCGAGGTAGATTTCAGGCGATCga > 1:1729862/1‑68 (MQ=255) cGCCTGGAAGCGCTTGAAGTTGTAATCCAGAGACGCATAACCACGCGAGGTAGATTTCAGGCGATCga > 1:1497403/1‑68 (MQ=255) cGCCTGGAAGCGCTTGAAGTTGTAATCCAGAGACGCATAACCACGCGAGGTAGATTTCAGGCGATCga > 1:1959828/1‑68 (MQ=255) cGCCTGGAAGCGCTTGAAGTTGTAATCCAGAGACGCATAACCACGCGAGGTAGATTTCAGGCGATCga > 1:2134673/1‑68 (MQ=255) cGCCTGGAAGCGCTTGAAGTTGTAATCCAGAGACGCATAACCACGCGAGGTAGATTTCAGGCGATCga > 1:2488569/1‑68 (MQ=255) cGCCTGGAAGCGCTTGAAGTTGTAATCCAGAGACGCATAACCACGCGAGGTAGATTTCAGGCGATCga > 1:1365279/1‑68 (MQ=255) cGCCTGGAAGCGCTTGAAGTTGTAATCCAGAGACGCATAACCACGCGAGGTAGATTTCAGGCGATCga > 1:1092595/1‑68 (MQ=255) cGCCTGGAAGCGCTTGAAGTTGTAATCCAGAGACGCATAACCACGCGAGGTAGATTTCAGGCGATCg > 1:2194455/1‑67 (MQ=255) gAAGCGCTTGAAGTTGTAATCCAGAGACGCATAACCACGCGAGGTAGATTTCAGGCGATCGaagaag > 1:789336/1‑67 (MQ=255) gAAGCGCTTGAAGTTGTAATCCAGAGACGCATAACCACGCGAGGTAGATTTCAGGCGATCGaagaag > 1:2165633/1‑67 (MQ=255) gAAGCGCTTGAAGTTGTAATCCAGAGACGCATAACCACGCGAGGTAGATTTCAGGCGATCGaagaag > 1:2336272/1‑67 (MQ=255) gAAGCGCTTGAAGTTGTAATCCAGAGACGCATAACCACGCGAGGTAGATTTCAGGCGATCGaagaag > 1:1372589/1‑67 (MQ=255) gAAGCGCTTGAAGTTGTAATCCAGAGACGCATAACCACGCGAGGTAGATTTCAGGCGATCGaagaag > 1:448362/1‑67 (MQ=255) gAAGCGCTTGAAGTTGTAATCCAGAGACGCATAACCACGCGAGGTAGATTTCAGGCGATCGaagaag > 1:1637568/1‑67 (MQ=255) aGCGCTTGAAGTTGTAATCCAGAGACGCATAACCACGCGAGGTAGATTTCAGGCGATCGAAGAAGTCg > 1:464608/1‑68 (MQ=255) cTTGATGTTGTAATCCAGAGACGCATAACCACGCGAGGTAGATTTCAGGCGATCGAAGAAGTCGAGcac < 1:138501/69‑1 (MQ=255) cTTGAAGTTGTAATCCAGAGACGCATAACCACGCGAGGTAGATTTCAGGCGATCGAAGAAGTCGAGcac < 1:1780116/69‑1 (MQ=255) cTTGAAGTTGTAATCCAGAGACGCATAACCACGCGAGGTAGATTTCAGGCGATCGAAGAAGTCGAGcac < 1:2630512/69‑1 (MQ=255) cTTGAAGTTGTAATCCAGAGACGCATAACCACGCGAGGTAGATTTCAGGCGATCGAAGAAGTCGAGcac < 1:3435604/69‑1 (MQ=255) cTTGAAGTTGTAATCCAGAGACGCATAACCACGCGAGGTAGATTTCAGGCGATCGAAGAAGTCGAGcac < 1:3345830/69‑1 (MQ=255) cTTGAAGTTGTAATCCAGAGACGCATAACCACGCGAGGTAGATTTCAGGCGATCGAAGAAGTCGAGcac < 1:2513860/69‑1 (MQ=255) cTTGAAGTTGTAATCCAGAGACGCATAACCACGCGAGGTAGATTTCAGGCGATCGAAGAAGTCGAGcac < 1:3207575/69‑1 (MQ=255) cTTGAAGTTGTAATCCAGAGACGCATAACCACGCGAGGTAGATTTCAGGCGATCGAAGAAGTCGAGcac < 1:1088795/69‑1 (MQ=255) cTTGAAGTTGTAATCCAGAGACGCATAACCACGCGAGGTAGATTTCAGGCGATCGAAGAAGTCGAGcac < 1:926070/69‑1 (MQ=255) cTTGAAGTTGTAATCCAGAGACGCATAACCACGCGAGGTAGATTTCAGGCGATCGAAGAAGTCGAGcac < 1:2536204/69‑1 (MQ=255) | AGCGCATCAACACGTTCACCGTTGATTAATACGTCTACACGTACCATGTCGGACGCCTGGAAGCGCTTGAAGTTGTAATCCAGAGACGCATAACCACGCGAGGTAGATTTCAGGCGATCGAAGAAGTCGAGCAC > minE/1738327‑1738460 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |