| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,747,578 | G→C | 38.6% | G143G (GGC→GGG) | yfiE ← | predicted DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,747,578 | 0 | G | C | 38.6% | 89.7 / 124.5 | 132 | G143G (GGC→GGG) | yfiE | predicted DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base G (35/46); new base C (47/4); total (82/50) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.33e-09 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.77e-01 | |||||||||||
Only 100 of 132 total aligned reads displayed.
CACCAGTGATTGTTCACCCAACTCTCGTCGATTCAGAGCATCATCATTCCCTACACGATAAAAGACGCCAACATCGGCCTCATCATTCAGTAGCGCATCACGGATCACGTAACAGTTCAGCG > minE/1747512‑1747633 | caccagTGATTGTTCACCCAACTCTCGTCGATTCAGAGCATCATCATTCCCTACACGATAAAAGACGcc < 1:2576043/69‑1 (MQ=255) accagTGATTGTTCACCCAACTCTCGTCGATTCAGAGCATCATCATTCCCTACACGATAAAAGACGcc < 1:487249/68‑1 (MQ=255) tGTTCACCCAACTCTCGTCGATTCAGAGCATCATCATTCCCTACACGATAAAAGACGCCAACATc > 1:2464342/1‑65 (MQ=255) tGTTCACCCAACTCTCGTCGATTCAGAGCATCATCATTCCCTACACGATAAAAGACGCCAACATCGGc > 1:1031023/1‑68 (MQ=255) tGTTCACCCAACTCTCGTCGATTCAGAGCATCATCATTCCCTACACGATAAAAGACGCCAACATCGGc > 1:2434838/1‑68 (MQ=255) tGTTCACCCAACTCTCGTCGATTCAGAGCATCATCATTCCCTACACGATAAAAGACGCCAACATCGGc < 1:1650934/68‑1 (MQ=255) tGTTCACCCAACTCTCGTCGATTCAGAGCATCATCATTCCCTACACGATAAAAGACGCCAACATCGGc > 1:1473130/1‑68 (MQ=255) aCTCTCGTCGGTTCAGAGCATCATCATTCCCTACGCGATAAAAAACCCCGACATCGGCCTCATCATTc > 1:2242494/1‑68 (MQ=255) aCTCTCGTCGGTTCAGAGCATCATCATTCCCTACGCGATAAAAAACCCCGACATCGGCCTCATCATTc > 1:2316768/1‑68 (MQ=255) aCTCTCGTCGGTTCAGAGCATCATCATTCCCTACGCGATAAAAAACCCCGACATCGGCCTCATCATTc > 1:1530822/1‑68 (MQ=255) aCTCTCGTCGGTTCAGAGCATCATCATTCCCTACGCGATAAAAAACCCCGACATCGGCCTCATCATTc > 1:2852429/1‑68 (MQ=255) aCTCTCGTCGGTTCAGAGCATCATCATTCCCTACGCGATAAAAAACCCCGACATCGGCCTCATCATTc > 1:1325284/1‑68 (MQ=255) aCTCTCGTCGGTTCAGAGCATCATCATTCCCTACGCGATAAAAAACCCCGACATCGGCCTCATCATTc > 1:1174855/1‑68 (MQ=255) aCTCTCGTCGGTTCAGAGCATCATCATTCCCTACGCGATAAAAAACCCCGACATCGGCCTCATCATTc > 1:853608/1‑68 (MQ=255) cgATTCAGAGCATCATCATTCCCTACACGATAAAAGACGCCAAc < 1:1255567/44‑1 (MQ=255) cgATTCAGAGCATCATCATTCCCTACACGATAAAAGACGCCAAc < 1:12927/44‑1 (MQ=255) cgATTCAGAGCATCATCATTCCCTACACGATAAAAGACGCCAAc < 1:3370230/44‑1 (MQ=255) cgATTCAGAGCATCATCATTCCCTACACGATAAAAGACGCCAAc < 1:1032070/44‑1 (MQ=255) cgATTCAGAGCATCATCATTCCCTACACGATAAAAGACGCCAAc < 1:1061014/44‑1 (MQ=255) cgATTCAGAGCATCATCATTCCCTACACGATAAAAGACGCCAAc < 1:2847627/44‑1 (MQ=255) ggttCAGAGCATCATCATTCCCTACGCGATAAAAAACCCCGACATCGGCCTcatcat > 1:2501389/3‑57 (MQ=255) ggttCAGAGCATCATCATTCCCTACGCGATAAAAAACCCCGACATCGGCCTcatcat > 1:2533883/3‑57 (MQ=255) ggttCAGAGCATCATCATTCCCTACGCGATAAAAAACCCCGACATCGGCCTcatcat > 1:1047416/3‑57 (MQ=255) ggttCAGAGCATCATCATTCCCTACGCGATAAAAAACCCCGACATCGGCCTcatcat > 1:263065/3‑57 (MQ=255) ggttCAGAGCATCATCATTCCCTACGCGATAAAAAACCCCGACATCGGCCTcatcat > 1:1918798/3‑57 (MQ=255) ggttCAGAGCATCATCATTCCCTACGCGATAAAAAACCCCGACATCGGCCTcatcat > 1:1194694/3‑57 (MQ=255) ggttCAGAGCATCATCATTCCCTACGCGATAAAAAACCCCGACATCGGCCTcatcat > 1:2971919/3‑57 (MQ=255) ggttCAGAGCATCATCATTCCCTACGCGATAAAAAACCCCGACATCGGCCTcatcat > 1:3058707/3‑57 (MQ=255) ggttCAGAGCATCATCATTCCCTACGCGATAAAAAACCCCGACATCGGCCTcatcat > 1:696015/3‑57 (MQ=255) ggttCAGAGCATCATCATTCCCTACGCGATAAAAAACCCCGACATCGGCCTcatcat > 1:1252244/3‑57 (MQ=255) gATTCAGAGCATCATCATTCCCTACACGATAAAAGACGCCAAc < 1:1874277/43‑1 (MQ=255) agagCATCATCATTCCCTACGCGATAAAAAACCCCGACATCGGCCTcatcat > 1:2400941/1‑52 (MQ=255) agagCATCATCATTCCCTACGCGATAAAAAACCCCGACATCGGCCTcatcat > 1:2468690/1‑52 (MQ=255) agagCATCATCATTCCCTACGCGATAAAAAACCCCGACATCGGCCTcatcat > 1:962791/1‑52 (MQ=255) agagCATCATCATTCCCTACGCGATAAAAAACCCCGACATCGGCCTcatcat > 1:924507/1‑52 (MQ=255) agagCATCATCATTCCCTACGCGATAAAAAACCCCGACATCGGCCTcatcat > 1:2590550/1‑52 (MQ=255) agagCATCATCATTCCCTACGCGATAAAAAACCCCGACATCGGCCTcatcat > 1:2617192/1‑52 (MQ=255) agagCATCATCATTCCCTACGCGATAAAAAACCCCGACATCGGCCTcatcat > 1:264616/1‑52 (MQ=255) agagCATCATCATTCCCTACGCGATAAAAAACCCCGACATCGGCCTcatcat > 1:845413/1‑52 (MQ=255) agagCATCATCATTCCCTACGCGATAAAAAACCCCGACATCGGCCTcatcat > 1:3322008/1‑52 (MQ=255) agagCATCATCATTCCCTACGCGATAAAAAACCCCGACATCGGCCTcatcat > 1:494096/1‑52 (MQ=255) agagCATCATCATTCCCTACGCGATAAAAAACCCCGACATCGGCCTcatcat > 1:1186125/1‑52 (MQ=255) agagCATCATCATTCCCTACGCGATAAAAAACCCCGACATCGGCCTcatcat > 1:1889685/1‑52 (MQ=255) agagCATCATCATTCCCTACGCGATAAAAAACCCCGACATCGGCCTcatcat > 1:1632550/1‑52 (MQ=255) agagCATCATCATTCCCTACGCGATAAAAAACCCCGACATCGGCCTcatcat > 1:162675/1‑52 (MQ=255) agagCATCATCATTCCCTACGCGATAAAAAACCCCGACATCGGCCTcatcat > 1:1916431/1‑52 (MQ=255) agagCATCATCATTCCCTACGCGATAAAAAACCCCGACATCGGCCTcatcat > 1:1468259/1‑52 (MQ=255) agagCATCATCATTCCCTACGCGATAAAAAACCCCGACATCGGCCTcatcat > 1:1971415/1‑52 (MQ=255) agagCATCATCATTCCCTACGCGATAAAAAACCCCGACATCGGCCTcatcat > 1:1204518/1‑52 (MQ=255) atcatTCCCTACACGATAAAAGACGCCAACATCgg > 1:1221281/1‑35 (MQ=255) atcatTCCCTACACGATAAAAGACGCCAACATCgg > 1:1829776/1‑35 (MQ=255) atcatTCCCTACACGATAAAAGACGCCAACATCGGc > 1:1341014/1‑36 (MQ=255) atcatTCCCTACACGATAAAAGACGCCAACATCGGc > 1:1279142/1‑36 (MQ=255) atcatTCCCTACACGATAAAAGACGCCAACATCGGc > 1:560404/1‑36 (MQ=255) atcatTCCCTACACGATAAAAGACGCCAACATCGGc > 1:2789786/1‑36 (MQ=255) atcatTCCCTACACGATAAAAGACGCCAACATCGGc > 1:838796/1‑36 (MQ=255) atcatTCCCTACACGATAAAAGACGCCAACATCGGc > 1:1012644/1‑36 (MQ=255) atcatTCCCTACACGATAAAAGACGCCAACATCGGc > 1:1784535/1‑36 (MQ=255) atcatTCCCTACACGATAAAAGACGCCAACATCGGc > 1:2549701/1‑36 (MQ=255) atcatTCCCTACACGATAAAAGACGCCAACATCGGc > 1:2504400/1‑36 (MQ=255) atcatTCCCTACACGATAAAAGACGCCAACATCGGc > 1:2005425/1‑36 (MQ=255) atcatTCCCTACACGATAAAAGACGCCAACATCGGc > 1:2309948/1‑36 (MQ=255) atcatTCCCTACACGATAAAAGACGCCAACATCGGc > 1:196852/1‑36 (MQ=255) atcatTCCCTACACGATAAAAGACGCCAACATCGGCCTCATCATTCAGTAGCGCATCACGGATCACGt > 1:349778/1‑68 (MQ=255) atcatTCCCTACACGATAAAAGACGCCAACATCGGCCTCATCATTCAGTAGCGCATCACGGATCACGt > 1:206685/1‑68 (MQ=255) atcatTCCCTACACGATAAAAGACGCCAACATCGGCCTCATCATTCAGTAGCGCATCACGGATCACGt > 1:3028960/1‑68 (MQ=255) atcatTCCCTACACGATAAAAGACGCCAACATCGGCCTCATCATTCAGTAGCGCATCACGGATCACGt > 1:1599006/1‑68 (MQ=255) atcatTCCCAACACGATAAAAGACGCCAACATCGGc > 1:2518021/1‑36 (MQ=255) tCCCTACACGATAAAAGACGCCAACATCGGCCTCATCATTCAGTAGCTCATCACGGATCACGTAACAGt < 1:1322905/69‑1 (MQ=255) tCCCTACACGATAAAAGACGCCAACATCGGCCTCATCATTCAGTAGCGCATCACGGATCACGTAACAGt < 1:618044/69‑1 (MQ=255) tCCCTACACGATAAAAGACGCCAACATCGGCCTCATCATTCAGTAGCGCATCACGGATCACGTAACAGt < 1:593864/69‑1 (MQ=255) tCCCTACACGATAAAAGACGCCAACATCGGCCTCATCATTCAGTAGCGCATCACGGATCACGTAACAGt < 1:2614056/69‑1 (MQ=255) tCCCTACACGATAAAAGACGCCAACATCGGCCTCATCATTCAGTAGCGCATCACGGATCACGTAACAGt < 1:440246/69‑1 (MQ=255) cccTACACGATAAAAGACGCCAACATCGGCCTCATCATTCAGTAGCGCATCACGGATCACGTAACAg > 1:2073328/1‑67 (MQ=255) cccTACACGATAAAAGACGCCAACATCGGCCTCATCATTCAGTAGCGCATCACGGATCACGTAACAg > 1:1977118/1‑67 (MQ=255) cccTACACGATAAAAGACGCCAACATCGGCCTCATCATTCAGTAGCGCATCACGGATCACGTAACAg > 1:3145984/1‑67 (MQ=255) cccTACACGATAAAAGACGCCAACATCGGCCTCATCATTCAGTAGCGCATCACGGATCACGTAACAg > 1:1753878/1‑67 (MQ=255) cccTACACGATAAAAGACGCCAACATCGGCCTCATCATTCAGTAGCGCATCACGGATCACGTAACAg > 1:3320560/1‑67 (MQ=255) cTACACGATAAAAGACGCCAACATCGGCCTCATCATTCAGTAGCGCATCACGGATCACGTAACAGTTc < 1:1907632/68‑1 (MQ=255) cTACACGATAAAAGACGCCAACATCGGCCTCATCATTCAGTAGCGCATCACGGATCACGTAACAGTTc < 1:809665/68‑1 (MQ=255) cTACACGATAAAAGACGCCAACATCGGCCTCATCATTCAGTAGCGCATCACGGATCACGTAACAGTTc < 1:1292749/68‑1 (MQ=255) cTACACGATAAAAGACGCCAACATCGGCCTCATCATTCAGTAGCGCATCACGGATCACGTAACAGTTc < 1:329419/68‑1 (MQ=255) cTACACGATAAAAGACGCCAACATCGGCCTCATCATTCAGTAGCGCATCACGGATCACGTAACAGTTc < 1:1087284/68‑1 (MQ=255) cTACACGATAAAAGACGCCAACATCGGCCTCATCATTCAGTAGCGCATCACGGATCACGTAACAGTTc < 1:907444/68‑1 (MQ=255) cacGATAAAAGACGCCAACATCGGCCTCATCATTCAGTAGCGCATCACgg < 1:131671/50‑1 (MQ=255) cacGATAAAAGACGCCAACATCGGCCTCATCATTCAGTAGCGCATCACgg < 1:1335650/50‑1 (MQ=255) cacGATAAAAGACGCCAACATCGGCCTCATCATTCAGTAGCGCATCACgg < 1:2489879/50‑1 (MQ=255) cacGATAAAAGACGCCAACATCGGCCTCATCATTCAGTAGCGCATCACgg < 1:2941136/50‑1 (MQ=255) cacGATAAAAGACGCCAACATCGGCCTCATCATTCAGTAGCGCATCACgg < 1:2662130/50‑1 (MQ=255) cacGATAAAAGACGCCAACATCGGCCTCATCATTCAGTAGCGCATCACgg < 1:782026/50‑1 (MQ=255) cacGATAAAAGACGCCAACATCGGCCTCATCATTCAGTAGCGCATCACGGATCACGTAACAGTTCAGCg > 1:3406233/1‑69 (MQ=255) cacGATAAAAGACGCCAACATCGGCCTCATCATTCAGTAGCGCATCACGGATCACGTAACAGTTCAGCg > 1:1343203/1‑69 (MQ=255) cacGATAAAAGACGCCAACATCGGCCTCATCATTAAGTAGCGCATCACGGATCACGTAACAGTTCAGCg > 1:2572134/1‑69 (MQ=255) acGATAAAAGACGCCAACATCGGCCTCATCATTCAGTAGCGCATCACgg < 1:2100123/49‑1 (MQ=255) cGATAAAAGACGCCAACATCGGCCTCATCATTCAGTAGCGCATCACGGATCACGTAACAGTTCAGCg < 1:2606464/67‑1 (MQ=255) cGATAAAAGACGCCAACATCGGCCTCATCATTCAGTAGCGCATCACGGATCACGTAACAGTTCAGCg < 1:2004890/67‑1 (MQ=255) aaaaaaCCCCGACATCGGCCTCATCATTCAGTAGCGCATCACGGATCACGTAACAGTTCAGc < 1:2097731/62‑1 (MQ=255) aaaaaaCCCCGACATCGGCCTCATCATTCAGTAGCGCATCACGGATCACGTAACAGTTCAGc < 1:3381080/62‑1 (MQ=255) aaaaaaCCCCGACATCGGCCTCATCATTCAGTAGCGCATCACGGATCACGTAACAGTTCAGc < 1:3063946/62‑1 (MQ=255) aaaaGACGCCAACATCGGCCTCATCATTCAGTAGCGCATCACGGATCACGTAACAGTTCAGCg < 1:1892280/63‑1 (MQ=255) | CACCAGTGATTGTTCACCCAACTCTCGTCGATTCAGAGCATCATCATTCCCTACACGATAAAAGACGCCAACATCGGCCTCATCATTCAGTAGCGCATCACGGATCACGTAACAGTTCAGCG > minE/1747512‑1747633 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |