| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,747,581 | A→G | 39.2% | V142V (GTT→GTC) | yfiE ← | predicted DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,747,581 | 0 | A | G | 39.2% | 62.6 / 89.6 | 130 | V142V (GTT→GTC) | yfiE | predicted DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base A (35/44); new base G (47/4); total (82/48) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.82e-09 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 | |||||||||||
Only 100 of 130 total aligned reads displayed.
TGTTCACCCAACTCTCGTCGATTCAGAGCATCATCATTCCCTACACGATAAAAGACGCCAACATCGGCCTCATCATTCAGTAGCGCATCACGGATCACGTAACAGTTCAGCG > minE/1747522‑1747633 | tGTTCACCCAACTCTCGTCGATTCAGAGCATCATCATTCCCTACACGATAAAAGACGCCAACATc > 1:2464342/1‑65 (MQ=255)tGTTCACCCAACTCTCGTCGATTCAGAGCATCATCATTCCCTACACGATAAAAGACGCCAACATCGGc > 1:1031023/1‑68 (MQ=255)tGTTCACCCAACTCTCGTCGATTCAGAGCATCATCATTCCCTACACGATAAAAGACGCCAACATCGGc > 1:2434838/1‑68 (MQ=255)tGTTCACCCAACTCTCGTCGATTCAGAGCATCATCATTCCCTACACGATAAAAGACGCCAACATCGGc < 1:19960/68‑1 (MQ=255)tGTTCACCCAACTCTCGTCGATTCAGAGCATCATCATTCCCTACACGATAAAAGACGCCAACATCGGc < 1:1650934/68‑1 (MQ=255)tGTTCACCCAACTCTCGTCGATTCAGAGCATCATCATTCCCTACACGATAAAAGACGCCAACATCGGc < 1:3314568/68‑1 (MQ=255)tGTTCACCCAACTCTCGTCGATTCAGAGCATCATCATTCCCTACACGATAAAAGACGCCAACATCGGc < 1:1274452/68‑1 (MQ=255) aCTCTCGTCGGTTCAGAGCATCATCATTCCCTACGCGATAAAAAACCCCGACATCGGCCTCATCATTc > 1:3049347/1‑68 (MQ=255) aCTCTCGTCGGTTCAGAGCATCATCATTCCCTACGCGATAAAAAACCCCGACATCGGCCTCATCATTc > 1:2242494/1‑68 (MQ=255) aCTCTCGTCGGTTCAGAGCATCATCATTCCCTACGCGATAAAAAACCCCGACATCGGCCTCATCATTc > 1:2516830/1‑68 (MQ=255) aCTCTCGTCGGTTCAGAGCATCATCATTCCCTACGCGATAAAAAACCCCGACATCGGCCTCATCATTc > 1:1721642/1‑68 (MQ=255) aCTCTCGTCGGTTCAGAGCATCATCATTCCCTACGCGATAAAAAACCCCGACATCGGCCTCATCATTc > 1:2852429/1‑68 (MQ=255) aCTCTCGTCGGTTCAGAGCATCATCATTCCCTACGCGATAAAAAACCCCGACATCGGCCTCATCATTc > 1:1530822/1‑68 (MQ=255) aCTCTCGTCGGTTCAGAGCATCATCATTCCCTACGCGATAAAAAACCCCGACATCGGCCTCATCATTc > 1:1413328/1‑68 (MQ=255) aCTCTCGTCGGTTCAGAGCATCATCATTCCCTACGCGATAAAAAACCCCGACATCGGCCTCATCATTc > 1:1325284/1‑68 (MQ=255) aCTCTCGTCGGTTCAGAGCATCATCATTCCCTACGCGATAAAAAACCCCGACATCGGCCTCATCATTc > 1:1295517/1‑68 (MQ=255) aCTCTCGTCGGTTCAGAGCATCATCATTCCCTACGCGATAAAAAACCCCGACATCGGCCTCATCATTc > 1:1174855/1‑68 (MQ=255) aCTCTCGTCGGTTCAGAGCATCATCATTCCCTACGCGATAAAAAACCCCGACATCGGCCTCATCATTc > 1:853608/1‑68 (MQ=255) cgATTCAGAGCATCATCATTCCCTACACGATAAAAGACGCCAAc < 1:3370230/44‑1 (MQ=255) cgATTCAGAGCATCATCATTCCCTACACGATAAAAGACGCCAAc < 1:12927/44‑1 (MQ=255) cgATTCAGAGCATCATCATTCCCTACACGATAAAAGACGCCAAc < 1:1255567/44‑1 (MQ=255) cgATTCAGAGCATCATCATTCCCTACACGATAAAAGACGCCAAc < 1:1032070/44‑1 (MQ=255) cgATTCAGAGCATCATCATTCCCTACACGATAAAAGACGCCAAc < 1:1061014/44‑1 (MQ=255) cgATTCAGAGCATCATCATTCCCTACACGATAAAAGACGCCAAc < 1:2847627/44‑1 (MQ=255) cgATTCAGAGCATCATCATTCCCTACACGATAAAAGACGCCAACATCGGCCTCATCATTCAGTAgcg < 1:640127/67‑1 (MQ=255) ggttCAGAGCATCATCATTCCCTACGCGATAAAAAACCCCGACATCGGCCTcatcat > 1:2126060/3‑57 (MQ=255) ggttCAGAGCATCATCATTCCCTACGCGATAAAAAACCCCGACATCGGCCTcatcat > 1:1047416/3‑57 (MQ=255) ggttCAGAGCATCATCATTCCCTACGCGATAAAAAACCCCGACATCGGCCTcatcat > 1:2501389/3‑57 (MQ=255) ggttCAGAGCATCATCATTCCCTACGCGATAAAAAACCCCGACATCGGCCTcatcat > 1:1918798/3‑57 (MQ=255) ggttCAGAGCATCATCATTCCCTACGCGATAAAAAACCCCGACATCGGCCTcatcat > 1:2533883/3‑57 (MQ=255) ggttCAGAGCATCATCATTCCCTACGCGATAAAAAACCCCGACATCGGCCTcatcat > 1:259098/3‑57 (MQ=255) ggttCAGAGCATCATCATTCCCTACGCGATAAAAAACCCCGACATCGGCCTcatcat > 1:276467/3‑57 (MQ=255) ggttCAGAGCATCATCATTCCCTACGCGATAAAAAACCCCGACATCGGCCTcatcat > 1:1194694/3‑57 (MQ=255) ggttCAGAGCATCATCATTCCCTACGCGATAAAAAACCCCGACATCGGCCTcatcat > 1:1595806/3‑57 (MQ=255) ggttCAGAGCATCATCATTCCCTACGCGATAAAAAACCCCGACATCGGCCTcatcat > 1:696015/3‑57 (MQ=255) ggttCAGAGCATCATCATTCCCTACGCGATAAAAAACCCCGACATCGGCCTcatcat > 1:1252244/3‑57 (MQ=255) ggttCAGAGCATCATCATTCCCTACGCGATAAAAAACCCCGACATCGGCCTcatcat > 1:3058707/3‑57 (MQ=255) gATTCAGAGCATCATCATTCCCTACACGATAAAAGACGCCAAc < 1:1874277/43‑1 (MQ=255) agagCATCATCATTCCCTACGCGATAAAAAACCCCGACATCGGCCTcatcat > 1:264616/1‑52 (MQ=255) agagCATCATCATTCCCTACGCGATAAAAAACCCCGACATCGGCCTcatcat > 1:2617192/1‑52 (MQ=255) agagCATCATCATTCCCTACGCGATAAAAAACCCCGACATCGGCCTcatcat > 1:2590550/1‑52 (MQ=255) agagCATCATCATTCCCTACGCGATAAAAAACCCCGACATCGGCCTcatcat > 1:845413/1‑52 (MQ=255) agagCATCATCATTCCCTACGCGATAAAAAACCCCGACATCGGCCTcatcat > 1:924507/1‑52 (MQ=255) agagCATCATCATTCCCTACGCGATAAAAAACCCCGACATCGGCCTcatcat > 1:962791/1‑52 (MQ=255) agagCATCATCATTCCCTACGCGATAAAAAACCCCGACATCGGCCTcatcat > 1:2400941/1‑52 (MQ=255) agagCATCATCATTCCCTACGCGATAAAAAACCCCGACATCGGCCTcatcat > 1:3322008/1‑52 (MQ=255) agagCATCATCATTCCCTACGCGATAAAAAACCCCGACATCGGCCTcatcat > 1:1468259/1‑52 (MQ=255) agagCATCATCATTCCCTACGCGATAAAAAACCCCGACATCGGCCTcatcat > 1:2071401/1‑52 (MQ=255) agagCATCATCATTCCCTACGCGATAAAAAACCCCGACATCGGCCTcatcat > 1:1186125/1‑52 (MQ=255) agagCATCATCATTCCCTACGCGATAAAAAACCCCGACATCGGCCTcatcat > 1:1632550/1‑52 (MQ=255) agagCATCATCATTCCCTACGCGATAAAAAACCCCGACATCGGCCTcatcat > 1:1204518/1‑52 (MQ=255) agagCATCATCATTCCCTACGCGATAAAAAACCCCGACATCGGCCTcatcat > 1:133237/1‑52 (MQ=255) atcatTCCCTACACGATAAAAGACGCCAACATCgg > 1:1221281/1‑35 (MQ=255) atcatTCCCTACACGATAAAAGACGCCAACATCgg > 1:1829776/1‑35 (MQ=255) atcatTCCCTACACGATAAAAGACGCCAACATCGGc > 1:1012644/1‑36 (MQ=255) atcatTCCCTACACGATAAAAGACGCCAACATCGGc > 1:1341014/1‑36 (MQ=255) atcatTCCCTACACGATAAAAGACGCCAACATCGGc > 1:1784535/1‑36 (MQ=255) atcatTCCCTACACGATAAAAGACGCCAACATCGGc > 1:560404/1‑36 (MQ=255) atcatTCCCTACACGATAAAAGACGCCAACATCGGc > 1:2309948/1‑36 (MQ=255) atcatTCCCTACACGATAAAAGACGCCAACATCGGc > 1:2005425/1‑36 (MQ=255) atcatTCCCTACACGATAAAAGACGCCAACATCGGc > 1:196852/1‑36 (MQ=255) atcatTCCCTACACGATAAAAGACGCCAACATCGGc > 1:838796/1‑36 (MQ=255) atcatTCCCTACACGATAAAAGACGCCAACATCGGCCTCATCATTCAGTAGCGCATCACGGATCACGt > 1:558241/1‑68 (MQ=255) atcatTCCCTACACGATAAAAGACGCCAACATCGGCCTCATCATTCAGTAGCGCATCACGGATCACGt > 1:3028960/1‑68 (MQ=255) atcatTCCCTACACGATAAAAGACGCCAACATCGGCCTCATCATTCAGTAGCGCATCACGGATCACGt > 1:349778/1‑68 (MQ=255) atcatTCCCTACACGATAAAAGACGCCAACATCGGCCTCATCATTCAGTAGCGCATCACGGATCACGt > 1:1599006/1‑68 (MQ=255) atcatTCCCAACACGATAAAAGACGCCAACATCGGc > 1:2518021/1‑36 (MQ=255) tCCCTACACGATAAAAGACGCCAACATCGGCCTCATCATTCAGTAGCTCATCACGGATCACGTAACAGt < 1:1322905/69‑1 (MQ=255) tCCCTACACGATAAAAGACGCCAACATCGGCCTCATCATTCAGTAGCGCATCACGGATCACGTAACAGt < 1:1925207/69‑1 (MQ=255) tCCCTACACGATAAAAGACGCCAACATCGGCCTCATCATTCAGTAGCGCATCACGGATCACGTAACAGt < 1:593864/69‑1 (MQ=255) tCCCTACACGATAAAAGACGCCAACATCGGCCTCATCATTCAGTAGCGCATCACGGATCACGTAACAGt < 1:2629641/69‑1 (MQ=255) tCCCTACACGATAAAAGACGCCAACATCGGCCTCATCATTCAGTAGCGCATCACGGATCACGTAACAGt < 1:2614056/69‑1 (MQ=255) tCCCTACACGATAAAAGACGCCAACATCGGCCTCATCATTCAGTAGCGCATCACGGATCACGTAACAGt < 1:1905425/69‑1 (MQ=255) tCCCTACACGATAAAAGACGCCAACATCGGCCTCATCATTCAGTAGCGCATCACGGATCACGTAACAGt < 1:440246/69‑1 (MQ=255) cccTACACGATAAAAGACGCCAACATCGGCCTCATCATTCAGTAGCGCATCACGGATCACGTAACAg > 1:3145984/1‑67 (MQ=255) cccTACACGATAAAAGACGCCAACATCGGCCTCATCATTCAGTAGCGCATCACGGATCACGTAACAg > 1:2073328/1‑67 (MQ=255) cccTACACGATAAAAGACGCCAACATCGGCCTCATCATTCAGTAGCGCATCACGGATCACGTAACAg > 1:1977118/1‑67 (MQ=255) cccTACACGATAAAAGACGCCAACATCGGCCTCATCATTCAGTAGCGCATCACGGATCACGTAACAGt < 1:425049/68‑1 (MQ=255) cTACACGATAAAAGACGCCAACATCGGCCTCATCATTCAGTAGCGCATCACGGATCACGTAACAGTTc < 1:1087284/68‑1 (MQ=255) cTACACGATAAAAGACGCCAACATCGGCCTCATCATTCAGTAGCGCATCACGGATCACGTAACAGTTc < 1:329419/68‑1 (MQ=255) cTACACGATAAAAGACGCCAACATCGGCCTCATCATTCAGTAGCGCATCACGGATCACGTAACAGTTc < 1:907444/68‑1 (MQ=255) cTACACGATAAAAGACGCCAACATCGGCCTCATCATTCAGTAGCGCATCACGGATCACGTAACAGTTc < 1:2333456/68‑1 (MQ=255) cTACACGATAAAAGACGCCAACATCGGCCTCATCATTCAGTAGCGCATCACGGATCACGTAACAGTTc < 1:809665/68‑1 (MQ=255) cTACACGATAAAAGACGCCAACATCGGCCTCATCATTCAGTAGCGCATCACGGATCACGTAACAGTTc < 1:1292749/68‑1 (MQ=255) cTACACGATAAAAGACGCCAACATCGGCCTCATCATTCAGTAGCGCATCACGGATCACGTAACAGTTc < 1:1907632/68‑1 (MQ=255) cacGATAAAAGACGCCAACATCGGCCTCATCATTCAGTAGCGCATCACgg < 1:2941136/50‑1 (MQ=255) cacGATAAAAGACGCCAACATCGGCCTCATCATTCAGTAGCGCATCACgg < 1:782026/50‑1 (MQ=255) cacGATAAAAGACGCCAACATCGGCCTCATCATTCAGTAGCGCATCACgg < 1:2489879/50‑1 (MQ=255) cacGATAAAAGACGCCAACATCGGCCTCATCATTCAGTAGCGCATCACgg < 1:2550701/50‑1 (MQ=255) cacGATAAAAGACGCCAACATCGGCCTCATCATTCAGTAGCGCATCACgg < 1:2662130/50‑1 (MQ=255) cacGATAAAAGACGCCAACATCGGCCTCATCATTCAGTAGCGCATCACGGATCACGTAACAGTTCAGc > 1:1863546/1‑68 (MQ=255) cacGATAAAAGACGCCAACATCGGCCTCATCATTCAGTAGCGCATCACGGATCACGTAACAGTTCAGCg > 1:1343203/1‑69 (MQ=255) cacGATAAAAGACGCCAACATCGGCCTCATCATTAAGTAGCGCATCACGGATCACGTAACAGTTCAGCg > 1:2572134/1‑69 (MQ=255) cGATAAAAGACGCCAACATCGGCCTCATCATTCAGTAGCGCATCACGGATCACGTAACAGTTCAGCg < 1:2004890/67‑1 (MQ=255) cGATAAAAGACGCCAACATCGGCCTCATCATTCAGTAGCGCATCACGGATCACGTAACAGTTCAGCg < 1:2862864/67‑1 (MQ=255) aaaaaaCCCCGACATCGGCCTCATCATTCAGTAGCGCATCACGGATCACGTAACAGTTCAGc < 1:2476810/62‑1 (MQ=255) aaaaaaCCCCGACATCGGCCTCATCATTCAGTAGCGCATCACGGATCACGTAACAGTTCAGc < 1:3381080/62‑1 (MQ=255) aaaaaaCCCCGACATCGGCCTCATCATTCAGTAGCGCATCACGGATCACGTAACAGTTCAGc < 1:2097731/62‑1 (MQ=255) aaaaGACGCCAACATCGGCCTCATCATTCAGTAGCGCATCACGGATCACGTAACAGTTCAGCg < 1:1892280/63‑1 (MQ=255) gACGCCAACATCGGCCTCATCATTCAGTAGCGCATCACgg < 1:3370295/40‑1 (MQ=255) | TGTTCACCCAACTCTCGTCGATTCAGAGCATCATCATTCCCTACACGATAAAAGACGCCAACATCGGCCTCATCATTCAGTAGCGCATCACGGATCACGTAACAGTTCAGCG > minE/1747522‑1747633 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |