| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,756,432 | T→C | 53.8% | N340N (AAT→AAC) | pssA → | phosphatidylserine synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,756,432 | 0 | T | C | 53.8% | 37.2 / 157.9 | 186 | N340N (AAT→AAC) | pssA | phosphatidylserine synthase |
| Reads supporting (aligned to +/- strand): ref base T (14/72); new base C (28/72); total (42/144) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.80e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 186 total aligned reads displayed.
TTGCCTTATCTCTATGAGATCAATCTGCGTCGTTTCCTGAGCCGTTTGCAGTATTACGTCAATACTGACCAGCTAGTGGTTCGGTTATGGAAAGATGACGACAACACCTATCACCTGAAAGGGATGTGG > minE/1756370‑1756498 | ttGCCTTATCTCTATGAGATCAATCTGCGTCGTTTCCTGAGTCGTTTACAGTATTACGTCAACACTGAc < 1:1008322/69‑1 (MQ=255)ttGCCTTATCTCTATGAGATCAATCTGCGTCGTTTCCTGAGTCGTTTACAGTATTACGTCAACACTGAc < 1:2309237/69‑1 (MQ=255)ttGCCTTATCTCTATGAGATCAATCTGCGTCGTTTCCTGAGTCGTTTACAGTATTACGTCAACACTGAc < 1:2692617/69‑1 (MQ=255)ttGCCTTATCTCTATGAGATCAATCTGCGTCGTTTCCTGAGTCGTTTACAGTATTACGTCAACACTGAc < 1:3271593/69‑1 (MQ=255)ttGCCTTATCTCTATGAGATCAATCTGCGTCGTTTCCTGAGTCGTTTACAGTATTACGTCAACACTGAc < 1:388911/69‑1 (MQ=255)ttGCCTTATCTCTATGAGATCAATCTGCGTCGTTTCCTGAGTCGTTTACAGTATTACGTCAACACTGAc < 1:144724/69‑1 (MQ=255)ttGCCTTATCTCTATGAGATCAATCTGCGTCGTTTCCTGAGTCGTTTACAGTATTACGTCAACACTGAc < 1:687378/69‑1 (MQ=255)ttGCCTTATCTCTATGAGATCAATCTGCGTCGTTTCCTGAGTCGTTTACAGTATTACGTCAACACTGAc < 1:524726/69‑1 (MQ=255) gCCTTATCTCTATGAGATCAATCTGCGTCGTTTCCTGAGCCGTTTGCAGTATTACGTCAATACTGACCa > 1:359234/1‑69 (MQ=255) gCCTTATCTCTATGAGATCAATCTGCGTCGTTTCCTGAGCCGTTTGCAGTATTACGTCAATACTGACCa > 1:2852016/1‑69 (MQ=255) ttATCTCTATGAGATCAATCTGCGTCGTTTCCTGAGCCGTTTGCAGTATTACGTCAATACTGACCAGc < 1:1455917/68‑1 (MQ=255) gagaTCAATCTGCGTCGTTTCCTGAGTCGTTTACAGTATTACGTCAACACTGACCAGTTAGTAGTTCg < 1:976787/68‑1 (MQ=255) gagaTCAATCTGCGTCGTTTCCTGAGTCGTTTACAGTATTACGTCAACACTGACCAGTTAGTAGTTCg < 1:3073973/68‑1 (MQ=255) gagaTCAATCTGCGTCGTTTCCTGAGTCGTTTACAGTATTACGTCAACACTGACCAGTTAGTAGTTCg < 1:1507944/68‑1 (MQ=255) gagaTCAATCTGCGTCGTTTCCTGAGTCGTTTACAGTATTACGTCAACACTGACCAGTTAGTAGTTCg < 1:423666/68‑1 (MQ=255) gagaTCAATCTGCGTCGTTTCCTGAGTCGTTTACAGTATTACGTCAACACTGACCAGTTAGTAGTTCg < 1:420768/68‑1 (MQ=255) gagaTCAATCTGCGTCGTTTCCTGAGTCGTTTACAGTATTACGTCAACACTGACCAGTTAGTAGTTCg < 1:2149545/68‑1 (MQ=255) cTGCGTCGTTTCCTGAGCCGTTTGCAGTATTACGTCAATACTGACCAGCTAGTGATTCGGTTATGGaaa < 1:2520135/69‑1 (MQ=255) tGCGTCGTTTCCTGAGTCGTTTACAGTATTACGTCAACACTGACCAGTTAGTAGTTCg < 1:1399586/58‑1 (MQ=255) tGCGTCGTTTCCTGAGCCGTTTGCAGTATTACGTCAATACTg < 1:2295622/42‑1 (MQ=255) tGCGTCGTTTCCTGAGCCGTTTGCAGTATTACGTCAATACTg < 1:2838370/42‑1 (MQ=255) tGCGTCGTTTCCTGAGCCGTTTGCAGTATTACGTCAATACTg < 1:88015/42‑1 (MQ=255) tGCGTCGTTTCCTGAGCCGTTTGCAGTATTACGTCAATACTg < 1:1145715/42‑1 (MQ=255) tGCGTCGTTTCCTGAGCCGTTTGCAGTATTACGTCAATACTg < 1:2210671/42‑1 (MQ=255) tGCGTCGTTTCCTGAGCCGTTTGCAGTATTACGTCAATACTg < 1:479980/42‑1 (MQ=255) gtTTACTGAGCCGTTTGCAGTATTACGTCAATACTGACCAGCTAGTGGTTCg > 1:2149458/1‑52 (MQ=255) ttCCTGAGCCGTTTGCAGTATTACGTCAATACTGACCAGCTAGTGGTTCGGTTATGGAAAGATgacgac < 1:2089991/69‑1 (MQ=255) ttCCTGAGCCGTTTGCAGTATTACGTCAATACTGACCAGCTAGTGGTTCGGTTATGGAAAGATgacgac < 1:851499/69‑1 (MQ=255) gAGCCGTTTGCAGTATTACGTCAATACTGACCAGCTAGTGGTTCGGTTATGGAAAGATGACGacaaca < 1:2202632/68‑1 (MQ=255) gAGCCGTTTGCAGTATTACGTCAATACTGACCAGCTAGTGGTTCGGTTATGGAAAGATGACGacaaca < 1:1249782/68‑1 (MQ=255) gAGCCGTTTGCAGTATTACGTCAATACTGACCAGCTAGTGGTTCGGTTATGGAAAGATGACGacaaca < 1:1805468/68‑1 (MQ=255) gAGCCGTTTGCAGTATTACGTCAATACTGACCAGCTAGTGGTTCGGTTATGGAAAGATGACGacaaca < 1:3355795/68‑1 (MQ=255) gAGCCGTTTGCAGTATTACGTCAATACTGACCAGCTAGTGGTTCGGTTATGGAAAGATGACGacaaca < 1:1152528/68‑1 (MQ=255) gAGCCGTTTGCAGTATTACGTCAATACTGACCAGCTAGTGGTTCGGTTATGGAAAGATGACGacaaca < 1:450893/68‑1 (MQ=255) gAGCCGTTTGCAGTATTACGTCAATACTGACCAGCTAGTGGTTCGGTTATGGAAAGATGACGacaaca < 1:589929/68‑1 (MQ=255) gTTTGCAGTATTACGTCAATACTGACCAGCTAGTGGTTCGGTTATGGAAAGATGACGACAACACCTATc < 1:3237586/69‑1 (MQ=255) gTTTGCAGTATTACGTCAATACTGACCAGCTAGTGGTTCGGTTATGGAAAGATGACGACAACACCTATc < 1:73759/69‑1 (MQ=255) tacAGTATTACGTCAACACTGACCAGTTAGTAGTTCGGTTATGGAAAGATGACGACAACACCt < 1:2938663/61‑1 (MQ=255) tacAGTATTACGTCAACACTGACCAGTTAGTAGTTCGGTTATGGAAAGATGACGACAACACCt < 1:3010465/61‑1 (MQ=255) tacAGTATTACGTCAACACTGACCAGTTAGTAGTTCGGTTATGGAAAGATGACGACAACACCt < 1:2736018/61‑1 (MQ=255) tacAGTATTACGTCAACACTGACCAGTTAGTAGTTCGGTTATGGAAAGATGACGACAACACCt < 1:3214553/61‑1 (MQ=255) tacAGTATTACGTCAACACTGACCAGTTAGTAGTTCGGTTATGGAAAGATGACGACAACACCt < 1:1011682/61‑1 (MQ=255) tacAGTATTACGTCAACACTGACCAGTTAGTAGTTCGGTTATGGAAAGATGACGACAACACCt < 1:1958922/61‑1 (MQ=255) tacAGTATTACGTCAACACTGACCAGTTAGTAGTTCGGTTATGGAAAGATGACGACAACACCt < 1:199133/61‑1 (MQ=255) tacAGTATTACGTCAACACTGACCAGTTAGTAGTTCGGTTATGGAAAGATGACGACAACACCt < 1:2050686/61‑1 (MQ=255) tGCAGTATTACGTCAATACTGACCAGCTAGTGGTTCGGTTATGGAAAGATGACGACAACACCTATCAc > 1:637118/1‑68 (MQ=255) tGCAGTATTACGTCAATACTGACCAGCTAGTGGTTCGGTTATGGAAAGATGACGACAACACCTATCAc > 1:2248073/1‑68 (MQ=255) acAGTATTACGTCAACACTGACCAGTTAGTAGTTCGGTTATGGAAAGATGACGACAACACCt < 1:430604/61‑1 (MQ=255) cAGTATTACGTCAACACTGACCAGTTAGTAGTTCGGTTATGGAAAGATGACGACAACACCTATCACCt < 1:2951638/68‑1 (MQ=255) cAGTATTACGTCAACACTGACCAGTTAGTAGTTCGGTTATGGAAAGATGACGACAACACCTATCACCt < 1:2881512/68‑1 (MQ=255) cAGTATTACGTCAACACTGACCAGTTAGTAGTTCGGTTATGGAAAGATGACGACAACACCTATCACCt < 1:1802203/68‑1 (MQ=255) cAGTATTACGTCAACACTGACCAGTTAGTAGTTCGGTTATGGAAAGATGACGACAACACCTATCACCt < 1:155502/68‑1 (MQ=255) cAGTATTACGTCAACACTGACCAGTTAGTAGTTCGGTTATGGAAAGATGACGACAACACCTATCACCt < 1:2649063/68‑1 (MQ=255) cAGTATTACGTCAACACTGACCAGTTAGTAGTTCGGTTATGGAAAGATGACGACAACACCTATCACCt < 1:608397/68‑1 (MQ=255) cAGTATTACGTCAACACTGACCAGTTAGTAGTTCGGTTATGGAAAGATGACGACAACACCTATCACCt < 1:2504962/68‑1 (MQ=255) cAGTATTACGTCAACACTGACCAGTTAGTAGTTCGGTTATGGAAAGATGACGACAACACCTATCACCt < 1:685084/68‑1 (MQ=255) cAGTATTACGTCAACACTGACCAGTTAGTAGTTCGGTTATGGAAAGATGACGACAACACCTATCACCt < 1:2357395/68‑1 (MQ=255) cAGTATTACGTCAACACTGACCAGTTAGTAGTTCGGTTATGGAAAGATGACGACAACACCTATCACCt < 1:938789/68‑1 (MQ=255) cAGTATTACGTCAACACTGACCAGTTAGTAGTTCGGTTATGGAAAGATGACGACAACACCTATCACCt < 1:2328344/68‑1 (MQ=255) cAGTATTACGTCAACACTGACCAGTTAGTAGTTCGGTTATGGAAAGATGACGACAACACCTATCACCt < 1:2276683/68‑1 (MQ=255) aGTATTACGTCAACACTGACCAGTTAGTAGTTCGGTTATGGAAAGATGACGacaa > 1:1130465/1‑55 (MQ=255) aGTATTACGTCAACACTGACCAGTTAGTAGTTCGGTTATGGAAAGATGACGacaa > 1:1595799/1‑55 (MQ=255) aGTATTACGTCAACACTGACCAGTTAGTAGTTCGGTTATGGAAAGATGACGacaa > 1:394084/1‑55 (MQ=255) aGTATTACGTCAACACTGACCAGTTAGTAGTTCGGTTATGGAAAGATGACGacaa > 1:1718658/1‑55 (MQ=255) aGTATTACGTCAACACTGACCAGTTAGTAGTTCGGTTATGGAAAGATGACGacaa > 1:177498/1‑55 (MQ=255) aGTATTACGTCAACACTGACCAGTTAGTAGTTCGGTTATGGAAAGATGACGacaa > 1:3136334/1‑55 (MQ=255) aGTATTACGTCAACACTGACCAGTTAGTAGTTCGGTTATGGAAAGATGACGacaa > 1:1869901/1‑55 (MQ=255) aGTATTACGTCAACACTGACCAGTTAGTAGTTCGGTTATGGAAAGATGACGacaa > 1:2262929/1‑55 (MQ=255) aGTATTACGTCAACACTGACCAGTTAGTAGTTCGGTTATGGAAAGATGACGacaa > 1:2751207/1‑55 (MQ=255) aGTATTACGTCAACACTGACCAGTTAGTAGTTCGGTTATGGAAAGATGACGacaa > 1:246826/1‑55 (MQ=255) aGTATTACGTCAACACTGACCAGTTAGTAGTTCGGTTATGGAAAGATGACGacaa > 1:2609418/1‑55 (MQ=255) aGTATTACGTCAACACTGACCAGTTAGTAGTTCGGTTATGGAAAGATGACGacaa > 1:2386005/1‑55 (MQ=255) aGTATTACGTCAACACTGACCAGTTAGTAGTTCGGTTATGGAAAGATGACGacaa > 1:2657044/1‑55 (MQ=255) aGTATTACGTCAACACTGACCAGTTAGTAGTTCGGTTATGGAAAGATGACGacaa > 1:2712524/1‑55 (MQ=255) aGTATTACGTCAACACTGACCAGTTAGTAGTTCGGTTATGGAAAGATGACGacaa > 1:2916145/1‑55 (MQ=255) aGTATTACGTCAACACTGACCAGTTAGTAGTTCGGTTATGGAAAGATGACGacaa > 1:2986943/1‑55 (MQ=255) ttACGTCAATACTGACCAGCTATTGGTTCGGTTATGGAAAGATGACGACAACACCTATCACCTGAAAgg < 1:1189713/69‑1 (MQ=255) ttACGTCAATACTGACCAGCTAGTGGTTCGGTTATGGAAAGATGACGACAACACCTATCACCTGAAAgg < 1:2790983/69‑1 (MQ=255) ttACGTCAATACTGACCAGCTAGTGGTTCGGTTATGGAAAGATGACGACAACACCTATCACCTGAAAgg < 1:1079087/69‑1 (MQ=255) ttACGTCAATACTGACCAGCTAGTGGTTCGGTTATGGAAAGATGACGACAACACCTATCACCTGAAAgg < 1:2345761/69‑1 (MQ=255) aCGTCAATACTGACCAGCTAGTGGTTCGGTTATGGAAAGATGACGACAACACCTATCACCTGAAAGGGa < 1:2812838/69‑1 (MQ=255) aCGTCAATACTGACCAGCTAGTGGTTCGGTTATGGAAAGATGACGACAACACCTATCACCTGAAAGGGa > 1:1568515/1‑69 (MQ=255) aCGTCAATACTGACCAGCTAGTGGTTCGGTTATGGAAAGATGACGACAACACCTATCACCTGAAAGGGa < 1:924465/69‑1 (MQ=255) aCGTCAATACTGACCAGCTAGTGGTTCGGTTATGGAAAGATGACGACAACACCTATCACCTGAAAGGGa < 1:460740/69‑1 (MQ=255) aCGTCAATACTGACCAGCTAGTGGTTCGGTTATGGAAAGATGACGACAACACCTATCACCTGAAAGGGa < 1:147300/69‑1 (MQ=255) aCGTCAATACTGACCAGCTAGTGGTTCGGTTATGGAAAGATGACGACAACACCTATCACCTGAAAGGGa < 1:1359223/69‑1 (MQ=255) gTCAACACTGACCAGTTAGTAGTTCGGTTATGGAAAGATGACGACAACACCTATCACCt < 1:1657480/59‑1 (MQ=255) cAATACTGACCAGCTAGTGGTTCGGTTATGGAAAGATGACGACAACACCTATCACCTGAAAGGGAtgtg < 1:767440/69‑1 (MQ=255) cAATACTGACCAGCTAGTGGTTCGGTTATGGAAAGATGACGACAACACCTATCACCTGAAAGGGAtgtg < 1:1184458/69‑1 (MQ=255) cAATACTGACCAGCTAGTGGTTCGGTTATGGAAAGATGACGACAACACCTATCACCTGAAAGGGAtgtg < 1:1897055/69‑1 (MQ=255) cAATACTGACCAGCTAGTGGTTCGGTTATGGAAAGATGACGACAACACCTATCACCTGAAAGGGAtgtg < 1:2746133/69‑1 (MQ=255) cAATACTGACCAGCTAGTGGTTCGGTTATGGAAAGATGACGACAACACCTATCACCTGAAAGGGAtgtg < 1:3307606/69‑1 (MQ=255) cAATACTGACCAGCTAGTGGTTCGGTTATGGAAAGATGACGACAACACCTATCACCTGAAAGGGAtgtg < 1:1928534/69‑1 (MQ=255) aaTACTGACCAGCTAGTGGTTCGGTTATGGAAAGATGACGACAACACCTATCACCTGAAAGGGATGTgg < 1:1251077/69‑1 (MQ=255) aaTACTGACCAGCTAGTGGTTCGGTTATGGAAAGATGACGACAACACCTATCACCTGAAAGGGATGTgg < 1:1735889/69‑1 (MQ=255) aaTACTGACCAGCTAGTGGTTCGGTTATGGAAAGATGACGACAACACCTATCACCTGAAAGGGATGTgg < 1:2354522/69‑1 (MQ=255) aaTACTGACCAGCTAGTGGTTCGGTTATGGAAAGATGACGACAACACCTATCACCTGAAAGGGATGTgg < 1:943667/69‑1 (MQ=255) aaTACTGACCAGCTAGTGGTTCGGTTATGGAAAGATGACGACAACACCTATCACCTGAAAGGGATGTgg < 1:1066702/69‑1 (MQ=255) aTACTGACCAGCTAGTGGTTCGGTTATGGAAAGATGACGACAACACCTATCACCTGAAAGGGAtgtg < 1:1967003/67‑1 (MQ=255) aTACTGACCAGCTAGTGGTTCGGTTATGGAAAGATGACGACAACACCTATCACCTGAAAGGGATGTgg < 1:1827047/68‑1 (MQ=255) | TTGCCTTATCTCTATGAGATCAATCTGCGTCGTTTCCTGAGCCGTTTGCAGTATTACGTCAATACTGACCAGCTAGTGGTTCGGTTATGGAAAGATGACGACAACACCTATCACCTGAAAGGGATGTGG > minE/1756370‑1756498 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |