| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,756,447 | G→A | 44.4% | V345V (GTG→GTA) | pssA → | phosphatidylserine synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,756,447 | 0 | G | A | 44.4% | 53.1 / 153.9 | 189 | V345V (GTG→GTA) | pssA | phosphatidylserine synthase |
| Reads supporting (aligned to +/- strand): ref base G (38/67); new base A (28/56); total (66/123) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.59e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.11e-01 | |||||||||||
Only 100 of 197 total aligned reads displayed.
GAGATCAATCTGCGTCGTTTCCTGAGCCGTTTGCAGTATTACGTCAATACTGACCAGCTAGTGGTTCGGTTATGGAAAGATGACGACAACACCTATCACCTGAAAGGGATGTGGGTTGATGATAAGTGGAT > minE/1756385‑1756515 | gagaTCAATCTGCGTCGTTTCCTGAGTCGTTTACAGTATTACGTCAACACTGACCAGTTAGTAGTTct < 1:939776/68‑2 (MQ=255)gagaTCAATCTGCGTCGTTTCCTGAGTCGTTTACAGTATTACGTCAACACTGACCAGTTAGTAGTTCg < 1:2188690/68‑1 (MQ=255)gagaTCAATCTGCGTCGTTTCCTGAGTCGTTTACAGTATTACGTCAACACTGACCAGTTAGTAGTTCg < 1:2260874/68‑1 (MQ=255)gagaTCAATCTGCGTCGTTTCCTGAGTCGTTTACAGTATTACGTCAACACTGACCAGTTAGTAGTTCg < 1:113575/68‑1 (MQ=255)gagaTCAATCTGCGTCGTTTCCTGAGTCGTTTACAGTATTACGTCAACACTGACCAGTTAGTAGTTCg < 1:2368325/68‑1 (MQ=255)gagaTCAATCTGCGTCGTTTCCTGAGTCGTTTACAGTATTACGTCAACACTGACCAGTTAGTAGTTCg < 1:2619505/68‑1 (MQ=255)gagaTCAATCTGCGTCGTTTCCTGAGTCGTTTACAGTATTACGTCAACACTGACCAGTTAGTAGTTCg < 1:3007318/68‑1 (MQ=255)gagaTCAATCTGCGTCGTTTCCTGAGTCGTTTACAGTATTACGTCAACACTGACCAGTTAGTAGTTCg < 1:3073973/68‑1 (MQ=255)gagaTCAATCTGCGTCGTTTCCTGAGTCGTTTACAGTATTACGTCAACACTGACCAGTTAGTAGTTCg < 1:420768/68‑1 (MQ=255)gagaTAAATCTGCGTCGTTTCCTGAGTCGTTTACAGTATTACGTCAACACTGACCAGTTAGTAGTTCg < 1:2275045/68‑1 (MQ=255) cTGCGTCGTTTCCTGAGCCGTTTGCAGTATTACGTCAATACTGACCAGCTAGTGGTTCGGTTATGGaaa < 1:600332/69‑1 (MQ=255) cTGCGTCGTTTCCTGAGCCGTTTGCAGTATTACGTCAATACTGACCAGCTAGTGATTCGGTTATGGaaa < 1:2520135/69‑1 (MQ=255) tGCGTCGTTTCCTGAGTCGTTTACAGTATTACGTCAACACTGACCAGTTAGTAGTTCg < 1:1399586/58‑1 (MQ=255) tGCGTCGTTTCCTGAGCCGTTTGCAGTATTACGTCAATACTGACCAGCTAGTGGTTCGGTTATGGaaa < 1:2357163/68‑1 (MQ=255) gtTTCCTGAGCCGTTTGCAGTATTACGTCAATACTGACCAGCTAGTGGTTCGGTTATGGAAAGATgac > 1:918884/1‑68 (MQ=255) gtTTACTGAGCCGTTTGCAGTATTACGTCAATACTGACCAGCTAGTGGTTCg > 1:2149458/1‑52 (MQ=255) ttCCTGAGCCGTTTGCAGTATTACGTCAATACTGACCAGCTAGTGGTTCGGTTATGGAAAGATgacgac < 1:851499/69‑1 (MQ=255) ttCCTGAGCCGTTTGCAGTATTACGTCAATACTGACCAGCTAGTGGTTCGGTTATGGAAAGATgacgac < 1:2089991/69‑1 (MQ=255) ttCCTGAGCCGTTTGCAGTATTACGTCAATACTGACCAGCTAGTGGTTCGGTTATGGAAAGATgacgac < 1:812227/69‑1 (MQ=255) gAGCCGTTTGCAGTATTACGTCAATACTGACCAGCTAGTGGTTCGGTTATGGAAAGATGACGacaaca < 1:1152528/68‑1 (MQ=255) gAGCCGTTTGCAGTATTACGTCAATACTGACCAGCTAGTGGTTCGGTTATGGAAAGATGACGacaaca < 1:1941929/68‑1 (MQ=255) gAGCCGTTTGCAGTATTACGTCAATACTGACCAGCTAGTGGTTCGGTTATGGAAAGATGACGacaaca < 1:1895564/68‑1 (MQ=255) gAGCCGTTTGCAGTATTACGTCAATACTGACCAGCTAGTGGTTCGGTTATGGAAAGATGACGacaaca < 1:3355795/68‑1 (MQ=255) gAGCCGTTTGCAGTATTACGTCAATACTGACCAGCTAGTGGTTCGGTTATGGAAAGATGACGacaaca < 1:1249782/68‑1 (MQ=255) gAGCCGTTTGCAGTATTACGTCAATACTGACCAGCTAGTGGTTCGGTTATGGAAAGATGACGacaaca < 1:1805468/68‑1 (MQ=255) gTTTGCAGTATTACGTCAATACTGACCAGCTAGTGGTTCGGTTATGGAAAGATGACGACAACACCTATc < 1:3237586/69‑1 (MQ=255) gTTTGCAGTATTACGTCAATACTGACCAGCTAGTGGTTCGGTTATGGAAAGATGACGACAACACCTATc < 1:2818523/69‑1 (MQ=255) tacAGTATTACGTCAACACTGACCAGTTAGTAGTTCGGTTATGGAAAGATGACGACAACACCt < 1:64711/61‑1 (MQ=255) tacAGTATTACGTCAACACTGACCAGTTAGTAGTTCGGTTATGGAAAGATGACGACAACACCt < 1:617887/61‑1 (MQ=255) tacAGTATTACGTCAACACTGACCAGTTAGTAGTTCGGTTATGGAAAGATGACGACAACACCt < 1:2282048/61‑1 (MQ=255) tacAGTATTACGTCAACACTGACCAGTTAGTAGTTCGGTTATGGAAAGATGACGACAACACCt < 1:3214553/61‑1 (MQ=255) tacAGTATTACGTCAACACTGACCAGTTAGTAGTTCGGTTATGGAAAGATGACGACAACACCt < 1:199133/61‑1 (MQ=255) tacAGTATTACGTCAACACTGACCAGTTAGTAGTTCGGTTATGGAAAGATGACGACAACACCt < 1:1011682/61‑1 (MQ=255) tGCAGTATTACGTCAATACTGACCAGCTAGTGGTTCGGTTATGGAAAGATGACGACAACACCTATCAc > 1:2703158/1‑68 (MQ=255) tGCAGTATTACGTCAATACTGACCAGCTAGTGGTTCGGTTATGGAAAGATGACGACAACACCTATCAc > 1:2940679/1‑68 (MQ=255) acAGTATTACGTCAACACTGACCAGTTAGTAGTTCGGTTATGGAAAGATGACGACAACACCt < 1:430604/61‑1 (MQ=255) cAGTATTACGTCAACACTGACCAGTTAGTAGTTCGGTTATGGAAAGATGACGACAACACCTATCACCt < 1:1042082/68‑1 (MQ=255) cAGTATTACGTCAACACTGACCAGTTAGTAGTTCGGTTATGGAAAGATGACGACAACACCTATCACCt < 1:2328344/68‑1 (MQ=255) cAGTATTACGTCAACACTGACCAGTTAGTAGTTCGGTTATGGAAAGATGACGACAACACCTATCACCt < 1:685084/68‑1 (MQ=255) cAGTATTACGTCAACACTGACCAGTTAGTAGTTCGGTTATGGAAAGATGACGACAACACCTATCACCt < 1:1981704/68‑1 (MQ=255) cAGTATTACGTCAACACTGACCAGTTAGTAGTTCGGTTATGGAAAGATGACGACAACACCTATCACCt < 1:155502/68‑1 (MQ=255) cAGTATTACGTCAACACTGACCAGTTAGTAGTTCGGTTATGGAAAGATGACGACAACACCTATCACCt < 1:144967/68‑1 (MQ=255) cAGTATTACGTCAACACTGACCAGTTAGTAGTTCGGTTATGGAAAGATGACGACAACACCTATCACCt < 1:1802203/68‑1 (MQ=255) aGTATTACGTCAACACTGACCAGTTAGTAGTTCGGTTATGGAAAGATgac > 1:2799500/1‑50 (MQ=255) aGTATTACGTCAACACTGACCAGTTAGTAGTTCGGTTATGGAAAGATGACGacaa > 1:1595799/1‑55 (MQ=255) aGTATTACGTCAACACTGACCAGTTAGTAGTTCGGTTATGGAAAGATGACGacaa > 1:2916145/1‑55 (MQ=255) aGTATTACGTCAACACTGACCAGTTAGTAGTTCGGTTATGGAAAGATGACGacaa > 1:3136334/1‑55 (MQ=255) aGTATTACGTCAACACTGACCAGTTAGTAGTTCGGTTATGGAAAGATGACGacaa > 1:2751207/1‑55 (MQ=255) aGTATTACGTCAACACTGACCAGTTAGTAGTTCGGTTATGGAAAGATGACGacaa > 1:1489310/1‑55 (MQ=255) aGTATTACGTCAACACTGACCAGTTAGTAGTTCGGTTATGGAAAGATGACGacaa > 1:26503/1‑55 (MQ=255) aGTATTACGTCAACACTGACCAGTTAGTAGTTCGGTTATGGAAAGATGACGacaa > 1:177498/1‑55 (MQ=255) aGTATTACGTCAACACTGACCAGTTAGTAGTTCGGTTATGGAAAGATGACGacaa > 1:2386005/1‑55 (MQ=255) aGTATTACGTCAACACTGACCAGTTAGTAGTTCGGTTATGGAAAGATGACGacaa > 1:558198/1‑55 (MQ=255) aGTATTACGTCAACACTGACCAGTTAGTAGTTCGGTTATGGAAAGATGACGacaa > 1:2350388/1‑55 (MQ=255) aGTATTACGTCAACACTGACCAGTTAGTAGTTCGGTTATGGAAAGATGACGacaa > 1:1274363/1‑55 (MQ=255) aGTATTACGTCAACACTGACCAGTTAGTAGTTCGGTTATGGAAAGATGACGacaa > 1:1127024/1‑55 (MQ=255) aGTATTACGTCAACACTGACCAGTTAGTAGTTCGGTTATGGAAAGATGACGacaa > 1:94965/1‑55 (MQ=255) aGTATTACGTCAACACTGACCAGTTAGTAGTTCGGTTATGGAAAGATGACGACAACACCTATCACCt < 1:2926369/67‑1 (MQ=255) aGTATTACGACAACACTGACCAGTTAGTAGTTCGGTTATGGAAAGATGACGacaa > 1:2856131/1‑55 (MQ=255) ttACGTCAATACTGACCAGCTATTGGTTCGGTTATGGAAAGATGACGACAACACCTATCACCTGATAgg < 1:41267/69‑1 (MQ=255) ttACGTCAATACTGACCAGCTAGTGGTTCGGTTATGGAAAGATGACGACAACACCTATCCCCTGAAAgg < 1:2209399/69‑1 (MQ=255) ttACGTCAATACTGACCAGCTAGTGGTTCGGTTATGGAAAGATGACGACAACACCTATCACCTGAAAgg > 1:452965/1‑69 (MQ=255) ttACGTCAATACTGACCAGCTAGTGGTTCGGTTATGGAAAGATGACGACAACACCTATCACCTGAAAgg < 1:2345761/69‑1 (MQ=255) ttACGTCAATACTGACCAGCTAGTGGTTCGGTTATGGAAAGATGACGACAACACCTATCACCTGAAAgg < 1:1079087/69‑1 (MQ=255) tACGTCAATACTGACCAGCTAGTGGTTCGGTTATGGAAAGATGACGACAACACCTATCACCTGAAAgg < 1:271994/68‑1 (MQ=255) aCGTCAATACTGACCAGCTAGTGGTTCGGTTATGGAAAGATGACGACAACACCTATCACCTGAAAGGGa < 1:2964861/69‑1 (MQ=255) aCGTCAATACTGACCAGCTAGTGGTTCGGTTATGGAAAGATGACGACAACACCTATCACCTGAAAGGGa < 1:932620/69‑1 (MQ=255) aCGTCAATACTGACCAGCTAGTGGTTCGGTTATGGAAAGATGACGACAACACCTATCACCTGAAAGGGa > 1:1568515/1‑69 (MQ=255) aCGTCAATACTGACCAGCTAGTGGTTCGGTTATGGAAAGATGACGACAACACCTATCACCTGAAAGGGa < 1:1359223/69‑1 (MQ=255) aCGTCAATACTGACCAGCTAGTGGTTCGGTTATGGAAAGATGACGACAACACCTATCACCTGAAAGGGa < 1:1965700/69‑1 (MQ=255) aCGTCAATACTGACCAGCTAGTGGTTCGGTTATGGAAAGATGACGACAACACCTATCACCTGAAAGGGa < 1:1851993/69‑1 (MQ=255) gTCAACACTGACCAGTTAGTAGTTCGGTTATGGAAAGATGACGACAACACCTATCACCt < 1:1657480/59‑1 (MQ=255) cAATACTGACCAGCTAGTGGTTCGGTTATGGAAAGATGACGACAACACCTATCACCTGAAAGGGAtgtg < 1:2237799/69‑1 (MQ=255) cAATACTGACCAGCTAGTGGTTCGGTTATGGAAAGATGACGACAACACCTATCACCTGAAAGGGAtgtg < 1:2746133/69‑1 (MQ=255) cAATACTGACCAGCTAGTGGTTCGGTTATGGAAAGATGACGACAACACCTATCACCTGAAAGGGAtgtg < 1:3307606/69‑1 (MQ=255) cAATACTGACCAGCTAGTGGTTCGGTTATGGAAAGATGACGACAACACCTATCACCTGAAAGGGAtgtg < 1:1184458/69‑1 (MQ=255) cAATACTGACCAGCTAGTGGTTCGGTTATGGAAAGATGACGACAACACCTATCACCTGAAAGGGAtgtg < 1:767440/69‑1 (MQ=255) cAATACTGACCAGCTAGTGGTTCGGTTATGGAAAGATGACGACAACACCTATCACCTGAAAGGGAtgtg < 1:1190439/69‑1 (MQ=255) aaTACTGACCAGCTAGTGGTTCGGTTATGGAAAGATGACGACAACACCTATCACCTGAAAGGGATGTgg < 1:1066702/69‑1 (MQ=255) aaTACTGACCAGCTAGTGGTTCGGTTATGGAAAGATGACGACAACACCTATCACCTGAAAGGGATGTgg < 1:1714798/69‑1 (MQ=255) aaTACTGACCAGCTAGTGGTTCGGTTATGGAAAGATGACGACAACACCTATCACCTGAAAGGGATGTgg < 1:1735889/69‑1 (MQ=255) aaTACTGACCAGCTAGTGGTTCGGTTATGGAAAGATGACGACAACACCTATCACCTGAAAGGGATGTgg < 1:385948/69‑1 (MQ=255) aaTACTGACCAGCTAGTGGTTCGGTTATGGAAAGATGACGACAACACCTATCACCTGAAAGGGATGTgg < 1:1251077/69‑1 (MQ=255) aaTACTGACCAGCTAGTGGTCCGGTTATGGAAAGATGACGACAACACCTATCACCTGAAAGGGATGTgg < 1:203566/69‑1 (MQ=255) aCTGACCAGCTAGTGGTTCGGTTATGGAAAGATGACGACAACACCTATCACCTGAAAGGGAt > 1:2054592/1‑62 (MQ=255) aCTGACCAGCTAGTGGTTCGGTTATGGAAAGATGACGACAACACCTATCACCTGAAAGGGATGTGGGtt > 1:121998/1‑69 (MQ=255) aCTGACCAGCTAGTGGTTCGGTTATGGAAAGATGACGACAACACCTATCACCTGAAAGGGATGTGGGtt > 1:2597088/1‑69 (MQ=255) gACCAGCTAGTGGTTCGGTTATGGAAAGATGACGACAACACCTATCACCTGAAAGGGATGTgg < 1:549653/63‑1 (MQ=255) aCCAGCTAGTGGTTCGGTTATGGAAAGATGACGACAACACCTATCACCTGAAAGGGATGTGGGTtgat > 1:425286/1‑68 (MQ=255) aCCAGCTAGTGGTTCGGTTATGGAAAGATGACGACAACACCTATCACCTGAAAGGGATGTGGGTtgat > 1:631186/1‑68 (MQ=255) aCCAGCTAGTGGTTCGGTTATGGAAAGATGACGACAACACCTATCACCTGAAAGGGATGTGGGTtgat > 1:2576016/1‑68 (MQ=255) aCCAGCTAGTGGTTCGGTTATGGAAAGATGACGACAACACCTATCACCTGAAAGGGATGTGGGTtgat > 1:1748140/1‑68 (MQ=255) aCCAGCTAGTGGTTCGGTTATGGAAAGATGACGACAACACCTATCACCTGAAAGGGATGTGGGTtgat > 1:3169465/1‑68 (MQ=255) aCCAGCTAGTGGTTCGGTTATGGAAAGATGACGACAACACCTATCACCTGAAAGGGATGTGGGTtgat > 1:2114507/1‑68 (MQ=255) aGCTAGTGGTTCGGTTATGGAAAGATGACGACAACACCTATCACCTGAAAGGGATGTGGGTTGATGATa < 1:1925737/69‑1 (MQ=255) aGTGGTTCGGTTATGGAAAGATGACGACAACACCTATCACCTGAAAGGGATGTGGGTTGATGATAAGTg < 1:3345179/69‑1 (MQ=255) ggTTCGGTTATGGAAAGATGACGACAACACCTATCACCTGAAAGGGATGTGGGTTGATGATa > 1:248907/1‑62 (MQ=255) ggTTCGGTTATGGAAAGATGACGACAACACCTATCACCTGAAAGGGATGTGGGTTGATGATAAGTGGAt > 1:1810127/1‑69 (MQ=255) ggTTCGGTTATGGAAAGATGACGACAACACCTATCACCTGAAAGGGATGTGGGTTGATGATAAGTGGAt > 1:266097/1‑69 (MQ=255) ggTTCGGTTATGGAAAGATGACGACAACACCTATCACCTGAAAGGGATGTGGGTTGATGATAAGTGGAt > 1:3032896/1‑69 (MQ=255) | GAGATCAATCTGCGTCGTTTCCTGAGCCGTTTGCAGTATTACGTCAATACTGACCAGCTAGTGGTTCGGTTATGGAAAGATGACGACAACACCTATCACCTGAAAGGGATGTGGGTTGATGATAAGTGGAT > minE/1756385‑1756515 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |