| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,758,115 | T→G | 28.0% | G106G (GGA→GGC) | kgtP ← | alpha‑ketoglutarate transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,758,115 | 0 | T | G | 28.0% | 179.9 / 95.7 | 150 | G106G (GGA→GGC) | kgtP | alpha‑ketoglutarate transporter |
| Reads supporting (aligned to +/- strand): ref base T (76/32); new base G (18/24); total (94/56) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.52e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 150 total aligned reads displayed.
AATGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTAT > minE/1758056‑1758177 | aatGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAacac > 1:2383072/1‑69 (MQ=255)aatGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAacac > 1:163157/1‑69 (MQ=255)aatGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAacac > 1:3106573/1‑69 (MQ=255)aatGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAacac > 1:3233876/1‑69 (MQ=255)aatGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAac > 1:908835/1‑67 (MQ=255) atGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGa > 1:1618831/1‑63 (MQ=255) atGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAacac > 1:1033122/1‑68 (MQ=255) atGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAacac > 1:1840072/1‑68 (MQ=255) atGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAacac > 1:2394554/1‑68 (MQ=255) atGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAacac > 1:2395450/1‑68 (MQ=255) atGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAacac > 1:2984031/1‑68 (MQ=255) atGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAaca > 1:2090657/1‑67 (MQ=255) atGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGAAGGCGATAACCAGGGATCCGAAacac > 1:1962223/1‑68 (MQ=255) ccGGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGTGATAACCAGCGATCCGAAACAcatca < 1:808794/69‑1 (MQ=255) ccGGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACAcatca < 1:1390658/69‑1 (MQ=255) ggAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACAcatcat < 1:3052845/68‑1 (MQ=255) ggAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATa < 1:3423622/69‑1 (MQ=255) ggAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATa < 1:3409796/69‑1 (MQ=255) ggAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATa < 1:49933/69‑1 (MQ=255) ggAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATa < 1:605003/69‑1 (MQ=255) ggAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATa < 1:1151722/69‑1 (MQ=255) gAGGCCAGGTACCGATTGTTTCGTAGCCTGGCAAACAGGCGATAACCAGTGAGCCGAAACACATCATa > 1:2810531/1‑68 (MQ=255) gAGCCCAGGTACCGATTGTTTCGTAGCCTGGCAAACAGGCGATAACCAGTGAGCCGAAACACATCATa > 1:2480926/1‑68 (MQ=255) gAGCCCAGGTACCGATTGTTTCGTAGCCTGGCAAACAGGCGATAACCAGTGAGCCGAAACACATCATa > 1:205484/1‑68 (MQ=255) gAGCCCAGGTACCGATTGTTTCGTAGCCTGGCAAACAGGCGATAACCAGTGAGCCGAAACACATCATa > 1:2254229/1‑68 (MQ=255) gAGCCCAGGTACCGATTGTTTCGTAGCCTGGCAAACAGGCGATAACCAGTGAGCCGAAACACATCATa > 1:98346/1‑68 (MQ=255) gAGCCCAGGTACCGATTGTTTCGTAGCCTGGCAAACAGGCGATAACCAGTGAGCCGAAACACATCATa > 1:2339003/1‑68 (MQ=255) gAGCCCAGGTACCGATTGTTTCGTAGCCTGGCAAACAGGCGATAACCAGTGAGCCGAAACACATCATa > 1:1000132/1‑68 (MQ=255) gAGCCCAGGTACCGATTGTTTCGTAGCCTGGCAAACAGGCGATAACCAGTGAGCCGAAACACATCATa > 1:714307/1‑68 (MQ=255) gAGCCCAGGTACCGATTGTTTCGTAGCCTGGCAAACAGGCGATAACCAGTGAGCCGAAACACATCATa > 1:3042130/1‑68 (MQ=255) gAGCCCAGGTACCGATTGTTTCGTAGCCTGGCAAACAGGCGATAACCAGTGAGCCGAAACACATCATa > 1:465003/1‑68 (MQ=255) gAGCCCAGGTACCGATTGTTTCGTAGCCTGGCAAACAGGCGATAACCAGTGAGCCGAAACACATCATa > 1:361066/1‑68 (MQ=255) gAGCCCAGGTACCGATTGTTTCGTAGCCTGGCAAACAGGCGATAACCAGTGAGCCGAAACACATCATa > 1:354405/1‑68 (MQ=255) gAGCCCAGGTACCGATTGTTTCGTAGCCTGGCAAACAGGCGATAACCAGTGAGCCGAAACACATCATa > 1:1545851/1‑68 (MQ=255) gAGCCCAGGTACCGATTGTTTCGTAGCCTGGCAAACAGGCGATAACCAGTGAGCCGAAACACATCATa > 1:1543418/1‑68 (MQ=255) gAGCCCAGGTACCGATTGTTTCGTAGCCTGGCAAACAGGCGATAACCAGTGAGCCGAAACACATCATa > 1:1340398/1‑68 (MQ=255) gAGCCCAGGTACCGATTGTTTCGTAGCCTGGCAAACAGGCGATAACCAGTGAGCCGAAACACATCATa > 1:172238/1‑68 (MQ=255) gAGCCCAGGTACCGATTGTTTCGTAGCCTGGCAAACAGGCGATAACCAGTGAGCCGAAACACATCATa > 1:1801961/1‑68 (MQ=255) gAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACAcatcat < 1:2059023/67‑1 (MQ=255) gCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCg < 1:2922221/54‑1 (MQ=255) gCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCg < 1:296161/54‑1 (MQ=255) gCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCg < 1:2533292/54‑1 (MQ=255) gCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCg < 1:2451412/54‑1 (MQ=255) gCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCg < 1:1503160/54‑1 (MQ=255) gCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCg < 1:1471157/54‑1 (MQ=255) gCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCg < 1:2070324/54‑1 (MQ=255) gCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCg < 1:1955310/54‑1 (MQ=255) aCCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATac > 1:1812239/1‑59 (MQ=255) aCCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGAt > 1:3344640/1‑67 (MQ=255) aCCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGAt > 1:381261/1‑67 (MQ=255) aCCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGAt > 1:1516202/1‑67 (MQ=255) aCCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGAt > 1:1214427/1‑67 (MQ=255) aCCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGAt > 1:1143021/1‑67 (MQ=255) aCCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGAt > 1:3006391/1‑67 (MQ=255) aCCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGAt > 1:1060043/1‑67 (MQ=255) aCCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGAt > 1:1610561/1‑67 (MQ=255) aCCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGAt > 1:2589359/1‑67 (MQ=255) ccTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACAcat > 1:3000637/1‑53 (MQ=255) ccTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAAc > 1:848054/1‑69 (MQ=255) aGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCa > 1:9438/1‑68 (MQ=255) aGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCa > 1:3257516/1‑68 (MQ=255) gTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATc > 1:2888167/1‑69 (MQ=255) gTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATc > 1:2831569/1‑69 (MQ=255) gTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATc > 1:2970663/1‑69 (MQ=255) gTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATc > 1:2240262/1‑69 (MQ=255) cTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATc > 1:2325413/1‑59 (MQ=255) cTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATc > 1:1502366/1‑59 (MQ=255) cTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATc > 1:1516996/1‑59 (MQ=255) cTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATc > 1:1135543/1‑59 (MQ=255) gcaggcGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGCGACCATg > 1:2487784/1‑69 (MQ=255) gcaggcGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGCGACCATg > 1:555529/1‑69 (MQ=255) gcaggcGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGCGACCATg > 1:1627797/1‑69 (MQ=255) gcaggcGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGCGACCATg > 1:2883244/1‑69 (MQ=255) gcGATAACCAGTGAGCCGAAACGCATCATACACACCGATa < 1:773832/40‑1 (MQ=25) gcGATAACCAGTGAGCCGAAACACATCATACACACCGATa < 1:3419414/40‑1 (MQ=37) gcGATAACCAGTGAGCCGAAACACATCATACACACCGATa < 1:861234/40‑1 (MQ=37) gcGATAACCAGTGAGCCGAAACACATCATACACACCGATa < 1:433618/40‑1 (MQ=37) gcGATAACCAGTGAGCCGAAACACATCATACACACCGATa < 1:98185/40‑1 (MQ=37) gcGATAACCAGTGAGCCGAAACACATCATACACACCGATa < 1:36931/40‑1 (MQ=37) gcGATAACCAGTGAGCCGAAACACATCATACACACCGATa < 1:1390191/40‑1 (MQ=37) gcGATAACCAGTGAGCCGAAACACATCATACACACCGATa < 1:1697799/40‑1 (MQ=37) gcGATAACCAGTGAGCCGAAACACATCATACACACCGATa < 1:3029291/40‑1 (MQ=37) gcGATAACCAGTGAGCCGAAACACATCATACACACCGATa < 1:3183467/40‑1 (MQ=37) gcGATAACCAGTGAGCCGAAACACATCATACACACCGATa < 1:2445207/40‑1 (MQ=37) cGATAAACAGTGAGCCGAAACACATCATACACACCGATa < 1:1123503/39‑1 (MQ=37) aTAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGCGACCATGTTTATCg < 1:1846454/69‑1 (MQ=255) aaCCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGCTACCATGTTTATCGGc > 1:576959/1‑69 (MQ=255) aaCCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGCGACCATGTTTATCGGc > 1:3284193/1‑69 (MQ=255) cAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTAt > 1:195369/1‑69 (MQ=255) cAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTAt > 1:1979196/1‑69 (MQ=255) cAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTAt > 1:2931814/1‑69 (MQ=255) cAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTAt > 1:2774746/1‑69 (MQ=255) cAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTAt > 1:2995611/1‑69 (MQ=255) cAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTAt > 1:749027/1‑69 (MQ=255) cAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTAt > 1:1484355/1‑69 (MQ=255) cAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTAt > 1:3330150/1‑69 (MQ=255) cAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTAt > 1:1340602/1‑69 (MQ=255) cAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTAt > 1:401237/1‑69 (MQ=255) cAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTAt > 1:2304681/1‑69 (MQ=255) cAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTAt > 1:284292/1‑69 (MQ=255) | AATGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTAT > minE/1758056‑1758177 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |