| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,764,751 | T→C | 36.8% | Q703Q (CAA→CAG) | clpB ← | protein disaggregation chaperone |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,764,751 | 0 | T | C | 36.8% | 60.0 / 65.1 | 103 | Q703Q (CAA→CAG) | clpB | protein disaggregation chaperone |
| Reads supporting (aligned to +/- strand): ref base T (26/39); new base C (19/19); total (45/58) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.11e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01 | |||||||||||
Only 100 of 103 total aligned reads displayed.
AATCAGATCGGAACCGAGGTTAGAGGTCATAATGACGACCGTATTACGGAAGTCGACCGTTCTCCCTTGCCCGTCAGTCAGACGCCCATCATCCAGTACCTGCAACAGAATGTTGAAGACATCCGGATGC > minE/1764685‑1764814 | aaTCAGATCGGAACCGAGGTTAGAGGTCATAATGACGAGCGTATTACGGAAGTCGACCGTTCTCCCCTg > 1:3330826/1‑69 (MQ=255)aaTCAGATCGGAACCGAGGTTAGAGGTCATAATGACGACCGTATTACGGAAGTCGACCGTTCTCCCCTg > 1:1768569/1‑69 (MQ=255)aaTCAGATCGGAACCGAGGTTAGAGGTCATAATGACGACCGTATTACGGAAGTCGACCGTTCTCCCCTg > 1:1873723/1‑69 (MQ=255)aaTCAGATCGGAACCGAGGTTAGAGGTCATAATGACGACCGTATTACGGAAGTCGACCGTTCTCCCCTg > 1:1230083/1‑69 (MQ=255)aaTCAGATCGGAACCGAGGTTAGAGGTCATAATGACGACCGTATTACGGAAGTCGACCGTTCTCCCCTg > 1:642004/1‑69 (MQ=255)aaTCAGATCGGAACCGAGGTTAGAGGTCATAATGACGACCGTATTACGGAAGTCGACCGTTCTCCCCTg > 1:1287903/1‑69 (MQ=255)aaTCAGATCGGAACCGAGGTTAGAGGTCATAATGACGACCGTATTACGGAAGTCGACCGTTCTCCCCTg > 1:639535/1‑69 (MQ=255)aaTCAGATCGGAACCGAGGTTAGAGGTCATAATGACGACCGTATTACGGAAGTCGACCGTTCTCCCCTg > 1:1900928/1‑69 (MQ=255)aaTCAGATCGGAACCGAGGTTAGAGGTCATAATGACGACCGTATTACGGAAGTCGACCGTTCTCCCCTg > 1:521228/1‑69 (MQ=255)aaTCAGATCGGAACCGAGGTTAGAGGTCATAATGACGACCGTATTACGGAAGTCGACCGTTCTCCCCTg > 1:242102/1‑69 (MQ=255) cAGATCGGAACCGAGGTTAGAGGGCATAATGACGACCGTATTACGGAAGTCGACCGTTCTCCCTTGccc < 1:732670/69‑1 (MQ=255) ggAACCGAGGTTAGAGGTCATATTGACGACCGTATTACGGAAGTCGACCGTTCTCCCTTGCCCgtcagt < 1:3032180/69‑1 (MQ=255) ggAACCGAGGTTAGAGGTCATAATGACGACCGTATTACGGAAGTCGACCGTTCTCCCTTGCCCgtcagt < 1:2755987/69‑1 (MQ=255) ggAACCGAGGTTAGAGGTCATAATGACGACCGTATTACGGAAGTCGACCGTTCTCCCTTGCCCgtcagt < 1:2860003/69‑1 (MQ=255) ggAACCGAGGTTAGAGGTCATAATGACGACCGTATTACGGAAGTCGACCGTTCTCCCTTGCCCgtcagt < 1:1833719/69‑1 (MQ=255) ggAACCGAGGTTAGAGGTCATAATGACGACCGTATTACGGAAGTCGACCGTTCTCCCTTGCCCgtcagt < 1:2882613/69‑1 (MQ=255) ggAACCGAGGTTAGAGGTCATAATGACGACCGTATTACGGAAGTCGACCGTTCTCCCTTGCCCgtcagt < 1:1338523/69‑1 (MQ=255) ggAACCGAGGTTAGAGGTCATAATGACGACCGTATTACGGAAGTCGACCGTTCTCCCTTGCCCgtcagt < 1:818162/69‑1 (MQ=255) ggAACCGAGGTTAGAGGTCATAATGACGACCGTATTACGGAAGTCGACCGTTCTCCCTTGCCCgtcagt < 1:894508/69‑1 (MQ=255) gAACCGAGGTTAGAGGTCATAATGACGACCGTATTACGGAAGTCGACCGTTCTCCCTTGCCCgtcagt < 1:2531738/68‑1 (MQ=255) gAACCGAGGTTAGAGGTCATAATGACGACCGTATTACGGAAGTCGACCGTTCTCCCTTGCCCgtcagt < 1:1871178/68‑1 (MQ=255) aGGTTAGAGGCCATAATGACGACCGTATTACGGAAGTCGACCGTTCTCCCTTGccc < 1:2330515/56‑1 (MQ=255) tAGAGGTCATAATGACGACCGTATTACGGAAGTCGACCGTTCTCCCTTGCCCgtcagt < 1:3391491/58‑1 (MQ=255) agGTCATAATGACGACCGTATTACGGAAGTCGACCGTTCTCCCCTGCCTGTCAGTCAGACGTCcatca < 1:435254/68‑1 (MQ=255) agGTCATAATGACGACCGTATTACGGAAGTCGACCGTTCTCCCCTGCCCGTCAGTCAGACGCCcatca < 1:2101153/68‑1 (MQ=255) agGTCATAATGACGACCGTATTACGGAAGTCGACCGTTCTCCCCTGCCCGTCAGTCAGACGCCcatca < 1:47496/68‑1 (MQ=255) agGTCATAATGACGACCGTATTACGGAAGTCGACCGTTCTCCCCTGCCCGTCAGTCAGACGCCcatca < 1:2930316/68‑1 (MQ=255) agGTCATAATGACGACCGTATTACGGAAGTCGACCGTTCTCCCCTGCCCGTCAGTCAGACGCCcatca < 1:2276886/68‑1 (MQ=255) agGTCATAATGACGACCGTATTACGGAAGTCGACCGTTCTCCCCTGCCCGTCAGTCAGACGCCcatca < 1:2838373/68‑1 (MQ=255) agGTCATAATGACGACCGTATTACGGAAGTCGACCGTTCTCCCCTGCCCGTCAGTCAGACGCCcatca < 1:1705289/68‑1 (MQ=255) agGTCATAATGACGACCGTATTACGGAAGTCGACCGTTCTCCCCTGCCCGTCAGTCAGACGCCcatca < 1:1521604/68‑1 (MQ=255) agGTCATAATGACGACCGTATTACGGAAGTCGACCGTTCTCCCCTGCCCGTCAGTCAGACGCCcatca < 1:576779/68‑1 (MQ=255) agGTCATAATGACGACCGTATTACGGAAGTCGACCGTTCTCCCCTGCCCGTCAGTCAGACGCCcatca < 1:697021/68‑1 (MQ=255) agGTCATAATGACGACCGTATTACGGAAGTCCACCGTTCTCCCCTGCCCGTCAGTCAGACGCCcatca < 1:3344030/68‑1 (MQ=255) gTCATAATGACGACCGTATTACGGAAGTCGACCGTTCTCCCTTGCCCGTCAGTCAGACGCCCATCATcc > 1:954370/1‑69 (MQ=255) cATAATGACGACCGTATTACGGAAGTCGACCGTTCTCCCTTGCCCGTCAGTCAGACGCCCATCATCCAg > 1:3407841/1‑69 (MQ=255) cATAATGACGACCGTATTACGGAAGTCGACCGTTCTCCCTTGCCCGTCAGTCAGACGCCCATCATCCAg > 1:2404301/1‑69 (MQ=255) cATAATGACGACCGTATTACGGAAGTCGACCGTTCTCCCTTGCCCGTCAGTCAGACGCCCATCATCCAg > 1:3052121/1‑69 (MQ=255) cATAATGACGACCGTATTACGGAAGTCGACCGTTCTCCCTTGCCCGTCAGTCAGACGCCCATCATCCAg > 1:350839/1‑69 (MQ=255) cATAATGACGACCGTATTACGGAAGTCGACCGTTCTCCCTTGCCCGTCAGTCAGACGCCCATCATCCAg > 1:1504525/1‑69 (MQ=255) cATAATGACGACCGTATTACGGAAGTCGACCGTTCTCCCTTGCCCGTCAGTCAGACGCCCATCATCCAg > 1:136143/1‑69 (MQ=255) aTAATGACGACCGTATTACGGAAGTCGACCGTTCTCCCTTTCCCGTCAGTCAGACGCCCATCATCCAGt < 1:763247/69‑1 (MQ=255) aTAATGACGACCGTATTACGGAAGTCGACCGTTCTCCCTTGCCCGTCAGTCAGACGCCcat > 1:1850837/1‑61 (MQ=255) aTAATGACGACCGTATTACGGAAGTCGACCGTTCTCCCTTGCCCGTCAGTCAGACGCCCATCATCCAGt > 1:3396843/1‑69 (MQ=255) aTAATGACGACCGTATTACGGAAGTCGACCGTTCTCCCTTGCCCGTCAGTCAGACGCCCATCATCCAGt > 1:2698944/1‑69 (MQ=255) aTAATGACGACCGTATTACGGAAGTCGACCGTTCTCCCTTGCCCGTCAGTCAGACGCCCATCATCCAGt > 1:2768438/1‑69 (MQ=255) aTAATGACGACCGTATTACGGAAGTCGACCGTTCTCCCTTGCCCGTCAGTCAGACGCCCATCATCCAGt > 1:549909/1‑69 (MQ=255) aTAATGACGACCGTATTACGGAAGTCGACCGTTCTCCCTTGCCCGTCAGTCAGACGCCCATCATCCAGt > 1:1049287/1‑69 (MQ=255) aTAATGACGACCGTATTACGGAAGTCGACCGTTCTCCCTTGCCCGTCAGTCAGACGCCCATCATCCAGt > 1:2695130/1‑69 (MQ=255) aTAATGACGACCGTATTACGGAAGTCGACCGTTCTCCCTTGCCCGTCAGTCAGACGCCCATCATCCAGt > 1:1186590/1‑69 (MQ=255) aTAATGACGACCGTATTACGGAAGTCGACCGTTCTCCCTTGCCCGTCAGTCAGACGCCCATCATCCAGt > 1:1149784/1‑69 (MQ=255) aTGACGACCGTATTACGGAAGTCGACCGTTCTCCCTTGCCCGTCAGTCAGACGCCCatcatc < 1:1106861/62‑1 (MQ=255) aTGACGACCGTATTACGGAAGTCGACCGTTCTCCCTTGCCCGTCAGTCAGACGCCCatcatc < 1:827797/62‑1 (MQ=255) aTGACGACCGTATTACGGAAGTCGACCGTTCTCCCTTGCCCGTCAGTCAGACGCCCATCATCCAGTAcc > 1:577275/1‑69 (MQ=255) aTGACGACCGTATTACGGAAGTCGACCGTTCTCCCTTGCCCGTCAGTCAGACGCCCATCATCCAGTAcc > 1:729739/1‑69 (MQ=255) aTGACGACCGTATTACGGAAGTCGACCGTTCTCCCTTGCCCGTCAGTCAGACGCCCATCATCCAGTAcc > 1:2881306/1‑69 (MQ=255) aTGACGACCGTATTACGGAAGTCGACCGTTCTCCCTTGCCCGTCAGTCAGACGCCCATCATCCAGTAcc > 1:2507599/1‑69 (MQ=255) aTGACGACCGTATTACGGAAGTCGACCGTTCTCCCTTGCCCGTCAGTCAGACGCCCATCATCCAGTAc > 1:2017055/1‑68 (MQ=255) gacgacCGTATTACGGAAGTCGACCGTTCTCCCTTGccc < 1:1841362/39‑1 (MQ=255) gacgacCGTATTACGGAAGTCGACCGTTCTCCCCTGCCCGTCAGTCAGACGCCcatca < 1:2077071/58‑1 (MQ=255) ccGTATTACGGAAGTCGACCGTTCTCCCCTGCCCGTCAGTCAGACGCCCATCATCCAGTACCTGCaa > 1:167726/1‑67 (MQ=255) ccGTATTACGGAAGTCGACCGTTCTCCCCTGCCCGTCAGTCAGACGCCCATCATCCAGTACCTGCAAc > 1:3083894/1‑68 (MQ=255) ccGTATTACGGAAGTCGACCGTTCTCCCCTGCCCGTCAGTCAGACGCCCATCATCCAGTACCTGCAAc > 1:2341997/1‑68 (MQ=255) ccGTATTACGGAAGTCGACCGTTCTCCCCTGCCCGTCAGTCAGACGCCCATCATCCAGTACCTGCAAc > 1:2833000/1‑68 (MQ=255) ccGTATTACGGAAGTCGACCGTTCTCCCCTGCCCGTCAGTCAGACGCCCATCATCCAGTACCTGCAAc > 1:669815/1‑68 (MQ=255) ccGTATTACGGAAGTCGACCGTTCTCCCCTGCCCGTCAGTCAGACGCCCATCATCCAGTACCTGCAAc > 1:3247186/1‑68 (MQ=255) ccGTATTACGGAAGTCGACCGTTCTCCCCTGCCCGTCAGTCAGACGCCCATCATCCAGTACCTGCAAc > 1:1129093/1‑68 (MQ=255) ccGTATTACGGAAGTCGACCGTTCTCCCCTGCCCGTCAGTCAGACGCCCATCATCCAGTACCTGCAAc > 1:2916283/1‑68 (MQ=255) ccGTATTACGGAAGTCGACCGTTCTCCCCTGCCCGTCAGTCAGACGCCCATCATCCAGTACCTGCAAc > 1:870534/1‑68 (MQ=255) aGTCGAGCGTTCTCCCTTGCCCGTCAGTCAGACGCCCATCATCCAGTACCTGCAACAGAATGTTGAAGa < 1:1608063/69‑1 (MQ=255) aGTCGACCGTTCTCCCTTGCCCGTCAGTCAGACGCGCATCATCCAGTACCTGCAACAGAATGTTGAAGa < 1:1906210/69‑1 (MQ=255) aGTCGACCGTTCTCCCTTGCCCGTCAGTCAGACGCCCATCATCCAGTACCTGCAACAGAATGTTGAAGa < 1:173172/69‑1 (MQ=255) aGTCGACCGTTCTCCCTTGCCCGTCAGTCAGACGCCCATCATCCAGTACCTGCAACAGAATGTTGAAGa < 1:166704/69‑1 (MQ=255) tCGACCGTTCTCCCTTGCCCGTCAGTCAGACGCCCATCATCCAGTACCTGCAACAGAATGTTGAAGACa < 1:2333400/69‑1 (MQ=255) tCGACCGTTCTCCCTTGCCCGTCAGTCAGACGCCCATCATCCAGTACCTGCAACAGAATGTTGAAGACa < 1:13814/69‑1 (MQ=255) tCGACCGTTCTCCCTTGCCCGTCAGTCAGACGCCCATCATCCAGTACCTGCAACAGAATGTTGAAGACa < 1:1733003/69‑1 (MQ=255) tCGACCGTTCTCCCTTGCCCGTCAGTCAGACGCCCATCATCCAGTACCTGCAACAGAATGTTGAAGACa < 1:1967331/69‑1 (MQ=255) tCGACCGTTCTCCCTTGCCCGTCAGTCAGACGCCCATCATCCAGTACCTGCAACAGAATGTTGAAGACa < 1:2955059/69‑1 (MQ=255) tCGACCGTTCTCCCTTGCCCGTCAGTCAGACGCCCATCATCCAGTACCTGCAACAGAATGTTGAAGACa < 1:1113963/69‑1 (MQ=255) tCGACCGTTCTCCCTTGCCCGTCAGTCAGACGCCCATCATCCAGTACCTGCAACAGAATGTTGAAGACa < 1:2906582/69‑1 (MQ=255) cGACCGTTCTCCCTTGCCCGTCAGTCAGACGCCCATCATcc < 1:382943/41‑1 (MQ=255) cGACCGTTCTCCCTTGCCCGTCAGTCAGACGCCCATCATCCAGTACCTGCAACAGAATGTTGAAGACa < 1:1291534/68‑1 (MQ=255) gACCGTTCTCCCTTGCCCGTCAGTCAGACGCCCATCATcc < 1:2105371/40‑1 (MQ=255) gACCGTTCTCCCTTGCCCGTCAGTCAGACGCCCATCATCCAGTACCTGCAACAGAATGTTGAAGACATc < 1:1278194/69‑1 (MQ=255) gACCGTTCTCCCTTGCCCGTCAGTCAGACGCCCATCATCCAGTACCTGCAACAGAATGTTGAAGACATc < 1:515053/69‑1 (MQ=255) aCCGTTCTCCCTTGCCCGTCAGTCAGACGCCCATCATCCAGTACCTGCAACAGAATGTTGAAGACATc < 1:3197488/68‑1 (MQ=255) ccGTTCTCCCTTGCCCGTCAGTCAGACGCCCATCATCCAGTACCTGCAACAGAATGTTGAAGACATCCg < 1:2602946/69‑1 (MQ=255) ccGTTCTCCCTTGCCCGTCAGTCAGACGCCCATCATCCAGTACCTGCAACAGAATGTTGAAGACATCCg < 1:596398/69‑1 (MQ=255) ccGTTCTCCCTTGCCCGTCAGTCAGACGCCCATCATCCAGTACCTGCAACAGAATGTTGAAGACATCCg < 1:3429473/69‑1 (MQ=255) cGTTCTCCCTTGCCCGTCAGTCAGACGCCCATCATCCAGTACCTGCAACAGAATGTTGAAGACATCCg < 1:2268070/68‑1 (MQ=255) cGTTCTCCCCTGCCCGTCAGTCAGACGCCCATCATCCAGTACCTGCAACAGAATGTTGa < 1:554456/59‑1 (MQ=255) cGTTCTCCCCTGCCCGTCAGTCAGACGCCCATCATCCAGTACCTGCAACAGAATGTTGa < 1:3400205/59‑1 (MQ=255) cGTTCTCCCCTGCCCGTCAGTCAGACGCCCATCATCCAGTACCTGCAACAGAATGTTGa < 1:1845283/59‑1 (MQ=255) cGTTCTCCCCTGCCCGTCAGTCAGACGCCCATCATCCAGTACCTGCAACAGAATGTTGa < 1:3025091/59‑1 (MQ=255) cGTTCTCCCCTGCCCGTCAGTCAGACGCCCATCATCCAGTACCTGCAACAGAATGTTGa < 1:2193186/59‑1 (MQ=255) cGTTCTCCCCTGCCCGTCAGTCAGACGCCCATCATCCAGTACCTGCAACAGAATGTTGa < 1:2332534/59‑1 (MQ=255) gTTCTCCCTTGCCCGTCAGTCAGACGCCcatcatg < 1:1095882/35‑2 (MQ=255) ctcCCTTGCCCGTCAGTCAGACGCCCATCATCCAGTACCTGCAACAGAATGTTGAAGACATCCGGATgc > 1:391120/1‑69 (MQ=255) ctcCCTTGCCCGTCAGTCAGACGCCCATCATCCAGTACCTGCAACAGAATGTTGAAGACATCCGGATgc > 1:373495/1‑69 (MQ=255) ctcCCTTGCCCGTCAGTCAGACGCCCATCATCCAGTACCTGCAACAGAATGTTGAAGACATCCGGATgc > 1:2679779/1‑69 (MQ=255) | AATCAGATCGGAACCGAGGTTAGAGGTCATAATGACGACCGTATTACGGAAGTCGACCGTTCTCCCTTGCCCGTCAGTCAGACGCCCATCATCCAGTACCTGCAACAGAATGTTGAAGACATCCGGATGC > minE/1764685‑1764814 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |