| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,765,891 | A→G | 59.0% | I323I (ATT→ATC) | clpB ← | protein disaggregation chaperone |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,765,891 | 0 | A | G | 59.0% | 29.2 / 49.9 | 73 | I323I (ATT→ATC) | clpB | protein disaggregation chaperone |
| Reads supporting (aligned to +/- strand): ref base A (12/18); new base G (25/18); total (37/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.57e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.82e-01 | |||||||||||
GTATCTTCAACAGAAGGCTCGGCAACAAACACTTTCTGGAAACGACGTTCCAGCGCAGCATCTTTTTCAATGTACTGGCGATATTCGTCAAGCGTCGTGGCACCTACGCAGTGCAATTCACCACGCGCCAG > minE/1765823‑1765953 | gTATCTTCAACAGAAGGCTCGGCAACAAACACTTTCTGGAAACGACGTTCCAGCGCAGCATCTTTTTCa > 1:491138/1‑69 (MQ=255)gTATCTTCAACAGAAGGCTCGGCAACAAACACTTTCTGGAAACGACGTTCCAGCGCAGCATCTTTTTCa > 1:1175761/1‑69 (MQ=255)gTATCTTCAACAGAAGGCTCGGCAACAAACACTTTCTGGAAACGACGTTCCAGCGCAGCATCTTTTTCa > 1:1668743/1‑69 (MQ=255)gTATCTTCAACAGAAGGCTCGGCAACAAACACTTTCTGGAAACGACGTTCCAGCGCAGCATCTTTTTCa > 1:2204944/1‑69 (MQ=255)gTATCTTCAACAGAAGGCTCGGCAACAAACACTTTCTGGAAACGACGTTCCAGCGCAGCATCTTTTTCa > 1:3168469/1‑69 (MQ=255)gTATCTTCAACAGAAGGCTCGGCAACAAACACTTTCTGGAAACGACGTTCCAGCGCAGCATCTTTTTCa > 1:2799053/1‑69 (MQ=255)gTATCTTCAACAGAAGGCTCGGCAACAAACACTTTCTGGAAACGACGTTCCAGCGCAGCATCTTTTTCa > 1:632514/1‑69 (MQ=255)gTATCTTCAACAGAAGGCTCGGCAACAAACACTTTCTGGAAACGACGTTCCAGCGCAGCATCTTTTTCa > 1:628586/1‑69 (MQ=255) cAACAGAAGGCTCGGCAACAAACACTTTCTGGAAACGACGTTCCAGCGCAGCATCTTTTTCAATGTACt > 1:2108815/1‑69 (MQ=255) cAACAGAAGGCTCGGCAACAAACACTTTCTGGAAACGACGTTCCAGCGCAGCATCTTTTTCAATGTACt > 1:1442056/1‑69 (MQ=255) aCAGAAGGCTCGGCAACAAACACTTTCTGGAAACGACGTTCCAGCTCAGCATCTTTTTCAATGTACTg < 1:3359366/68‑1 (MQ=255) aCAGAAGGCTCGGCAACAAACACTTTCTGGAAACGACGTTCCAGCGCAGCATCTTTTTCAATGTACTg < 1:321078/68‑1 (MQ=255) cAGAAGGCTCGGCAACAAACACTTTCTGGAAACGACGTTCCAGCGCAGCATCTTTTTCAATg > 1:1996182/1‑62 (MQ=255) cAGAAGGCTCGGCAACAAACACTTTCT‑‑AAACGACGTTCCAGCGCAGCATCTTTTTCAATGTACTGGCGa < 1:455214/69‑1 (MQ=255) gAAGGCTCGGCAACAAACACTTTCTGGAAACGACGTTCCAGCGCAGCATCTTTTTCAATGTACTGGGGa < 1:888892/69‑1 (MQ=255) gAAGGCTCGGCAACAAACACTTTCTGGAAACGACGTTCCAGCGCAGCATCTTTTTCAATGTACTGGCGa < 1:3022860/69‑1 (MQ=255) gAAGGCTCGGCAACAAACACTTTCTGGAAACGACGTTCCAGCGCAGCATCTTTTTCAATGTACTGGCGa < 1:2114683/69‑1 (MQ=255) gAAGGCTCGGCAACAAACACTTTCTGGAAACGACGTTCCAGCGCAGCATCTTTTTCAATGTACTGGCGa < 1:2120743/69‑1 (MQ=255) gAAGGCTCGGCAACAAACACTTTCTGGAAACGACGTTCCAGCGCAGCATCTTTTTCAATGTACTGGCGa < 1:720406/69‑1 (MQ=255) gAAGGCTCGGCAACAAACACTTTCTGGAAACGACGTTCCAGCGCAGCATCTTTTTCAATGTACTGGCGa < 1:1425775/69‑1 (MQ=255) gAAGGCTCGGCAACAAACACTTTCTGGAAACGACGTTCCAGCGCAGCATCTTTTTCAATGTACTGGCGa < 1:2747232/69‑1 (MQ=255) gAAGGCTCGGCAACAAACACTTTCTGGAAACGACGTTCCAGCGCAGCATCTTTTTCAATGTACTGGCGa < 1:999893/69‑1 (MQ=255) aaGGCTCGGCAACAAACACTTTCTGGAAACGACGTTCCAGCGCAGCATCTTTTTCAATTTACTGGCGa < 1:2659171/68‑1 (MQ=255) ggCTCGGCAAAAAAAACTTTCTGGAAACGACGTTCCAGCGCAGCATCTTTTTCAATGTACTGGCGa < 1:1883589/66‑1 (MQ=255) caacaaACACTTTCTGGAAACGACGTTCCAGCGCAGCATCTTTTTCAATGTACTg < 1:3002075/55‑1 (MQ=255) tttCTGGAAACGACGTTCCAGCGCAGCATCTTTTTCGATGTACTGGCGATATTCGTCAAGCGTCGTGGc > 1:2828834/1‑69 (MQ=255) tttCTGGAAACGACGTTCCAGCGCAGCATCTTTTTCGATGTACTGGCGATATTCGTCAAGCGTCGTGGc > 1:2476557/1‑69 (MQ=255) tttCTGGAAACGACGTTCCAGCGCAGCATCTTTTTCGATGTACTGGCGATATTCGTCAAGCGTCGTGGc > 1:1032234/1‑69 (MQ=255) tttCTGGAAACGACGTTCCAGCGCAGCATCTTTTTCGATGTACTGGCGATATTCGTCAAGCGTCGTGGc > 1:2466129/1‑69 (MQ=255) tttCTGGAAACGACGTTCCAGCGCAGCATCTTTTTCGATGTACTGGCGATATTCGTCAAGCGTCGTGGc > 1:2922885/1‑69 (MQ=255) tttCTGGAAACGACGTTCCAGCGCAGCATCTTTTTCGATGTACTGGCGATATTCGTCAAGCGTCGTGGc > 1:388981/1‑69 (MQ=255) tttCTGGAAACGACGTTCCAGCGCAGCATCTTTTTCGATGTACTGGCGATATTCGTCAAGCGTCGTGGc > 1:576576/1‑69 (MQ=255) tttCTGGAAACGACGTTCCAGCGCAGCATCTTTTTCGATGTACTGGCGATATTCGTCAAGCGTCGTGGc > 1:685293/1‑69 (MQ=255) tttCTGGAAACGACGTTCCAGCGCAGCATCTTTTTCGATGTACTGGCGATATTCGTCAAGCGTCGTGGc > 1:862296/1‑69 (MQ=255) tttCTGGAAACGACGTTCCAGCGCAGCATCTTTTTCGATGTACTGGCGATATTCGTCAAGCGTCGTGGc > 1:912662/1‑69 (MQ=255) tttCTGGAAACGACGTTCCAGCGCAGCATCTTTTTCGATGTACTGGCGATATTCGTCAAGCGTCGTGGc > 1:92387/1‑69 (MQ=255) tttCTGGAAACGACGTTCCAGCGCAGCATCTTTTTCGATGTACTGGCGATATTCGTCAAGCGTCGTGGc > 1:993639/1‑69 (MQ=255) tttCTGGAAACGACGTTCCAGCGCAGCATCTTTTTCGATGTACTGGCGATATTCGTCAAGCGTCGTGGc > 1:2425863/1‑69 (MQ=255) tttCTGGAAACGACGTTCCAGCGCAGCATCTTTTTCGATGTACTGGCGATATTCGTCAAGCGTCGTGGc > 1:1863590/1‑69 (MQ=255) tttCTGGAAACGACGTTCCAGCGCAGCATCTTTTTCGATGTACTGGCGATATTCGTCAAGCGTCGTGGc > 1:1243475/1‑69 (MQ=255) tttCTGGAAACGACGTTCCAGCGCAGCATCTTTTTCGATGTACTGGCGATATTCGTCAAGCGTCGTGGc > 1:2212380/1‑69 (MQ=255) tttCTGGAAACGACGTTCCAGCGCAGCATCTTTTTCGATGTACTGGCGATATTCGTCAAGCGTCGTGGc > 1:1594288/1‑69 (MQ=255) tttCTGGAAACGACGTTCCAGCGCAGCATCTTTTTCGATGTACTGGCGATATTCGTCAAGCGTCGTGGc > 1:2120056/1‑69 (MQ=255) ggAAACGACGTTCCAGCGCAGCATCTTTTTCGATGTACTGGCGATATTCGTCAAGCGTCGTGGCACCTa > 1:2528369/1‑69 (MQ=255) ggAAACGACGTTCCAGCGCAGCATCTTTTTCGATGTACTGGCGATATTCGTCAAGCGTCGTGGCACCTa > 1:1613703/1‑69 (MQ=255) ggAAACGACGTTCCAGCGCAGCATCTTTTTCGATGTACTGGCGATATTCGTCAAGCGTCGTGGCACCTa > 1:2408291/1‑69 (MQ=255) ggAAACGACGTTCCAGCGCAGCATCTTTTTCGATGTACTGGCGATATTCGTCAAGCGTCGTGGCACCTa > 1:2695928/1‑69 (MQ=255) ggAAACGACGTTCCAGCGCAGCATCTTTTTCGATGTACTGGCGATATTCGTCAAGCGTCGTGGCACCTa > 1:1400259/1‑69 (MQ=255) ggAAACGACGTTCCAGCGCAGCATCTTTTTCGATGTACTGGCGATATTCGTCAAGCGTCGTGGCACCTa > 1:1580662/1‑69 (MQ=255) ggAAACGACGTTCCAGCGCAGCATCTTTTTCGATGTACTGGCGATATTCGTCAAGCGTCGTGGCACCTa > 1:1505018/1‑69 (MQ=255) aaaCTACGTTCCAGCGCAGCATCTTTTTCAATTTACTGGCGata < 1:705861/44‑1 (MQ=38) aaCGACGTTCCAGCGCAGCATCTTTTTCGATGTACTGGCGATATTCGTCAAGCGTCGTGGCACCTa < 1:1017056/66‑1 (MQ=255) aaCGACGTTCCAGCGCAGCATCTTTTTCGATGTACTGGCGATATTCGTCAAGCGTCGTGGCACCTa < 1:1460298/66‑1 (MQ=255) aaCGACGTTCCAGCGCAGCATCTTTTTCGATGTACTGGCGATATTCGTCAAGCGTCGTGGCACCTa < 1:606769/66‑1 (MQ=255) aaCGACGTTCCAGCGCAGCATCTTTTTCGATGTACTGGCGATATTCGTCAAGCGTCGTGGCACCTa < 1:1248055/66‑1 (MQ=255) aaCGACGTTCCAGCGCAGCATCTTTTTCGATGTACTGGCGATATTCGTCAAGCGTCGTGGCACCTa < 1:553581/66‑1 (MQ=255) aaCGACGTTCCAGCGCAGCATCTTTTTCGATGTACTGGCGATATTCGTCAAGCGTCGTGGCACCTa < 1:1710891/66‑1 (MQ=255) aaCGACGTTCCAGCGCAGCATCTTTTTCGATGTACTGGCGATATTCGTCAAGCGTCGTGGCACCTa < 1:244328/66‑1 (MQ=255) aaCGACGTTCCAGCGCAGCATCTTTTTCGATGTACTGGCGATATTCGTCAAGCGTCGTGGCACCTa < 1:454009/66‑1 (MQ=255) aaCGACGTTCCAGCGCAGCATCTTTTTCGATGTACTGGCGATATTCGTCAAGCGTCGTGGCACCTa < 1:1727812/66‑1 (MQ=255) aaCGACGTTCCAGCGCAGCATCTTTTTCGATGTACTGGCGATATTCGTCAAGCGTCGTGGCACCTa < 1:2582888/66‑1 (MQ=255) aaCGACGTTCCAGCGCAGCATCTTTTTCGATGTACTGGCGATATTCGTCAAGCGTCGTGGCACCTa < 1:3264168/66‑1 (MQ=255) aaCGACGTTCCAGCGCAGCATCTTTTTCGATGTACTGGCGATATTCGTCAAGCGTCGTGGCACCTa < 1:2607896/66‑1 (MQ=255) aaCGACGTTCCAGCGCAGCATCTTTTTCGATGTACTGGCGATATTCGTCAAGCGTCGTGGCACCTa < 1:2396708/66‑1 (MQ=255) aaCGACGTTCCAGCGCAGCATCTTTTTCGATGTACTGGCGATATTCGTCAAGCGTCGTGGCACCTa < 1:2922546/66‑1 (MQ=255) aaCGACGTTCCAGCGCAGCATCTTTTTCGATGTACTGGCGATATTCGTCAAGCGTCGTGGCACCTa < 1:2122057/66‑1 (MQ=255) aaCGACGTTCCAGCGCAGCATCTTTTTCGATGTACTGGCGATATTCGTCAAGCGTCGTGGCACCTa < 1:307156/66‑1 (MQ=255) aaCGACGTTCCAGCGCAGCATCTTTTTCGATGTACTGGCGATATTCGTCAAGCGTCGTGGCACCTa < 1:302607/66‑1 (MQ=255) aaCGACGTTCCAGCGCAGCATCTTTTTCGATGTACTGGCGATATTCGTCAAGCGTCGTGGCACCTa < 1:1999802/66‑1 (MQ=255) ccAGCGCAGCATCTTTTTCAATGTACTGGCGATATTCGTCAAGCGTCGTGGCACCTACGCAGTGCAAtt < 1:2488877/69‑1 (MQ=255) ccAGCGCAGCATCTTTTTCAATGTACTGGCGATATTCGTCAAGCGTCGTGGCACCTACGCAGTGCAAtt < 1:1126299/69‑1 (MQ=255) ccAGCGCAGCATCTTTTTCAATGTACTGGCGATATTCGTCAAGCGTCGTGGCACCTACGCAGTGCAAtt < 1:3385402/69‑1 (MQ=255) tttttCAATGTACTGGCGATATTCGTCAAGCGTCGTGGCACCTACGCAGTGCAATTCACCACGCGCGAg > 1:705220/1‑69 (MQ=255) tttttCAATGTACTGGCGATATTCGTCAAGCGTCGTGGCACCTACGCAGTGCAATTCACCACGCGCCAg > 1:192786/1‑69 (MQ=255) tttttCAATGTACTGGCGATATTCGTCAAGCGTCGTGGCACCTACGCAGTGCAATTCACCACGCGCCAg > 1:3187280/1‑69 (MQ=255) tttttCAATGTACTGGCGATATTCGTCAAGCGTCGTGGCACCTACGCAGTGCAATTCACCACGCGCCAg > 1:2171259/1‑69 (MQ=255) tttttCAATGTACTGGCGATATTCGTCAAGCGTCGTGGCACCTACGCAGTGCAATTCACCACGCGCCAg > 1:1680097/1‑69 (MQ=255) tttttCAATGTACTGGCGATATTCGTCAAGCGTCGTGGCACCTACGCAGTGCAATTCACCACGCGCCAg > 1:1263835/1‑69 (MQ=255) tttttCAATGTACTGGCGATATTCGTCAAGCGTCGTGGCACCTACGCAGTGCAATTCACCACGCGCCAg > 1:3191829/1‑69 (MQ=255) tttttCAATGTACTGGCGATATTCGTCAAGCGTCGTGGCACCTACGCAGTGCAATTCACCACGCGCCAg > 1:3312689/1‑69 (MQ=255) tttttCAATGTACTGGCGATATTCGTCAAGCGTCGTGGCACCTACGCAGTGCAATTCACCACGCGCCAg > 1:1759748/1‑69 (MQ=255) | GTATCTTCAACAGAAGGCTCGGCAACAAACACTTTCTGGAAACGACGTTCCAGCGCAGCATCTTTTTCAATGTACTGGCGATATTCGTCAAGCGTCGTGGCACCTACGCAGTGCAATTCACCACGCGCCAG > minE/1765823‑1765953 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |