| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,767,900 | T→C | 30.4% | A266A (GCA→GCG) | rluD ← | 23S rRNA pseudouridine synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,767,900 | 0 | T | C | 30.4% | 96.2 / 50.6 | 105 | A266A (GCA→GCG) | rluD | 23S rRNA pseudouridine synthase |
| Reads supporting (aligned to +/- strand): ref base T (47/26); new base C (26/6); total (73/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.08e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 105 total aligned reads displayed.
ATAAAGACGCAGCATGGTTGCATGTAGCGCCTGGCGGTCAAACTTACGCAGCGTGGAGATAAATGCTTCCGAAGCACCTTTTGGCGGACGCGGACGGCCACCATAAACCGGATCGCCCACCAGCGGATGA > minE/1767837‑1767966 | aTAAAGACGCAGCATGGTTGCATGTAGCGCCTGGCGGTCAAACTTACGCAGCGTGGAGATAAATGCtt > 1:3181985/1‑68 (MQ=255)aTAAAGACGCAGCATGGTTGCATGTAGCGCCTGGCGGTCAAACTTACGCAGCGTGGAGATAAATGCtt > 1:2731356/1‑68 (MQ=255) aCGCAGCATGGTTGCATGTAGCGCCTGGCGGTCAAACTTACGCAGCGTGGAGATAAATGCTTCCGAAGc < 1:385550/69‑1 (MQ=255) tGGTTGCATGTAGCGCCTGGCGGTCAAACTTACGCAGCGTGGAGATAAATGCTTCCGAAGCACCTTTTg > 1:3367383/1‑69 (MQ=255) tGGTTGCATGTAGCGCCTGGCGGTCAAACTTACGCAGCGTGGAGATAAATGCTTCCGAAGCACCTTTTg > 1:1875849/1‑69 (MQ=255) tGGTTGCATGTAGCGCCTGGCGGTCAAACTTACGCAGCGTGGAGATAAATGCTTCCGAAGCACCTTTTg > 1:205919/1‑69 (MQ=255) tGGTTGCATGTAGCGCCTGGCGGTCAAACTTACGCAGCGTGGAGATAAATGCTTCCGAAGCACCTTTTg > 1:2408783/1‑69 (MQ=255) tGGTTGCATGTAGCGCCTGGCGGTCAAACTTACGCAGCGTGGAGATAAATGCTTCCGAAGCACCTTTTg > 1:1585760/1‑69 (MQ=255) tGGTTGCATGTAGCGCCTGGCGGTCAAACTTACGCAGCGTGGAGATAAATGCTTCCGAAGCACCTTTTg > 1:326579/1‑69 (MQ=255) tGGTTGCATGTAGCGCCTGGCGGTCAAACTTACGCAGCGTGGAGATAAATGCTTCCGAAGCACCTTTTg > 1:1234761/1‑69 (MQ=255) tGGTTGCATGTAGCGCCTGGCGGTCAAACTTACGCAGCGTGGAGATAAATGCTTCCGAAGCACCTTTTg > 1:1169664/1‑69 (MQ=255) tGCATGTAGCGCCTGGCGGTCAAACTTACGCAGCGTGGAGATAAATGCTTCCGAAGCACCTTTTGgcgg > 1:335392/1‑69 (MQ=255) tAGCGCCTGGCGGTCAAACTTACGCAGCGTGGAGATAAATGCTTCCGAAGCACCTTTTGgcggacgcgg > 1:2137089/1‑69 (MQ=255) tAGCGCCTGGCGGTCAAACTTACGCAGCGTGGAGATAAATGCTTCCGAAGCACCTTTTGgcggacgcgg > 1:1021003/1‑69 (MQ=255) tAGCGCCTGGCGGTCAAACTTACGCAGCGTGGAGATAAATGCTTCCGAAGCACCTTTTGgcggacgcgg > 1:2751178/1‑69 (MQ=255) tAGCGCCTGGCGGTCAAACTTACGCAGCGTGGAGATAAATGCTTCCGAAGCACCTTTTGgcggacgcgg > 1:2518702/1‑69 (MQ=255) tAGCGCCTGGCGGTCAAACTTACGCAGCGTGGAGATAAATGCTTCCGAAGCACCTTTTGgcggacgcgg > 1:3152469/1‑69 (MQ=255) tAGCGCCTGGCGGTCAAACTTACGCAGCGTGGAGATAAATGCTTCCGAAGCACCTTTTGgcggacgcgg > 1:2162216/1‑69 (MQ=255) tAGCGCCTGGCGGTCAAACTTACGCAGCGTGGAGATAAATGCTTCCGAAGCACCTTTTGgcggacgcgg > 1:2158391/1‑69 (MQ=255) tAGCGCCTGGCGGTCAAACTTACGCAGCGTGGAGATAAATGCTTCCGAAGCACCTTTTGgcggacgcgg > 1:210110/1‑69 (MQ=255) tAGCGCCTGGCGGTCAAACTTACGCAGCGTGGAGATAAATGCTTCCGAAGCACCTTTTGgcggacgcgg > 1:3142971/1‑69 (MQ=255) tAGCGCCTGGCGGTCAAACTTACGCAGCGTGGAGATAAATGCTTCCGAAGCACCTTTTGgcggacgcgg > 1:1857741/1‑69 (MQ=255) tAGCGCCTGGCGGTCAAACTTACGCAGCGTGGAGATAAATGCTTCCGAAGCACCTTTTGgcggacgcgg > 1:901622/1‑69 (MQ=255) tAGCGCCTGGCGGTCAAACTTACGCAGCGTGGAGATAAATGCTTCCGAAGCACCTTTTGgcggacgcgg > 1:1175286/1‑69 (MQ=255) tAGCGCCTGGCGGTCAAACTTACGCAGCGTGGAGATAAATGCTTCCGAAGCACCTTTTGgcggacgcgg > 1:1809948/1‑69 (MQ=255) tAGCGCCTGGCGGTCAAACTTACGCAGCGTGGAGATAAATGCTTCCGAAGCACCTTTTGgcggacgcgg > 1:1200332/1‑69 (MQ=255) aGCGCCTGGCGGTCAAACTTACGCAGCGTGGAGATAAATGCTTCCGAAGCACCTTTTGgcggacgcgga > 1:2509351/1‑69 (MQ=255) aGCGCCTGGCGGTCAAACTTACGCAGCGTGGAGATAAATGCTTCCGAAGCACCTTTTGgcggacgcgga > 1:2922011/1‑69 (MQ=255) aGCGCCTGGCGGTCAAACTTACGCAGCGTGGAGATAAATGCTTCCGAAGCACCTTTTGgcggacgcgg > 1:626311/1‑68 (MQ=255) cAAACTTACGCAGCGTGGAGATAAATGCTTCCGAAGCACCTTTTGGCGGCCGCGGACGGCCACCATaaa > 1:1949561/1‑69 (MQ=255) cAAACATACGCAGCGTGGAGATAAATGCTTCCGAAGCACCTTTTGgcggacg > 1:2134087/1‑52 (MQ=255) aaaCTTACGCAGCGTGGAGATAAACGCTTCCGAAGCACCttt > 1:872583/1‑42 (MQ=255) aaaCTTACGCAGCGTGGAGATAAACGCTTCCGAAGCACCTTTTGgcggacgcgg > 1:875279/1‑54 (MQ=255) aaaCTTACGCAGCGTGGAGATAAACGCTTCCGAAGCACCTTTTGgcggacgcg > 1:2057640/1‑53 (MQ=255) aaaCTTACGCAGCGTGGAGATAAACGCTTCCGAAGCACCTTTTGgcggacgcg > 1:2612951/1‑53 (MQ=255) aaaCTTACGCAGCGTGGAGATAAACGCTTCCGAAGCACCTTTTGgcggacgcg > 1:2321250/1‑53 (MQ=255) aaaCTTACGCAGCGTGGAGATAAACGCTTCCGAAGCACCTTTTGgcggacgcg > 1:2205784/1‑53 (MQ=255) aaaCTTACGCAGCGTGGAGATAAACGCTTCCGAAGCACCTTTTGgcggacgcg > 1:1411284/1‑53 (MQ=255) aaaCTTACGCAGCGTGGAGATAAACGCTTCCGAAGCACCTTTTGgcggacgcg > 1:2165461/1‑53 (MQ=255) aaaCTTACGCAGCGTGGAGATAAACGCTTCCGAAGCACCTTTTGgcggacgcg > 1:1251030/1‑53 (MQ=255) aaaCTTACGCAGCGTGGAGATAAACGCTTCCGAAGCACCTTTTGgcggacgcg > 1:178447/1‑53 (MQ=255) aaaCTTACGCAGCGTGGAGATAAACGCTTCCGAAGCACCTTTTGgcggacgcg > 1:3159212/1‑53 (MQ=255) aaaCTTACGCAGCGTGGAGATAAACGCTTCCGAAGCACCTTTTGgcggacgcg > 1:518272/1‑53 (MQ=255) aaaCTTACGCAGCGTGGAGATAAACGCTTCCGAAGCACCTTTTGgcggacgcg > 1:2103319/1‑53 (MQ=255) aaaCTTACGCAGCGTGGAGATAAACGCTTCCGAAGCACCTTTTGgcggacgcg > 1:1826473/1‑53 (MQ=255) aaaCTTACGCAGCGTGGAGATAAACGCTTCCGAAGCACCTTTTGgcggacgc > 1:2288670/1‑52 (MQ=255) aaaCTTACGCAGCGTGGAGATAAACGCTTCCGAAGCACCTTTTGGCGGACGCGGACGGCCACCATAAAc > 1:2724932/1‑69 (MQ=255) aaaCTTACGCAGCGTGGAGATAAACGCTTCCGAAGCACCTTTTGGCGGACGCGGACGGCCACCATAAAc > 1:1582851/1‑69 (MQ=255) aaaCTTACGCAGCGTGGAGATAAACGCTTCCGAAGCACCTTTTGGCGGACGCGGACGGCCACCATAAAc > 1:3136847/1‑69 (MQ=255) aaaCTTACGCAGCGTGGAGATAAACGCTTCCGAAGCACCTTTTGGCGGACGCGGACGGCCACCATAAAc > 1:2342165/1‑69 (MQ=255) aaaCTTACGCAGCGTGGAGATAAACGCTTCCGAAGCACCTTTTGGCGGACGCGGACGGCCACCATAAAc > 1:389419/1‑69 (MQ=255) aaaCTTACGCAGCGTGGAGATAAACGCTTCCGAAGCACCTTTTGGCGGACGCGGACGGCCACCATAAAc > 1:2179587/1‑69 (MQ=255) aaaCTTACGCAGCGTGGAGATAAACGCTTCCGAAGCACCTTTTGGCGGACGCGGACGGCCACCATAAAc > 1:586398/1‑69 (MQ=255) aaaCTTACGCAGCGTGGAGATAAACGCTTCCGAAGCACCTTTTGGCGGACGCGGACGGCCACCATAAAc > 1:2081271/1‑69 (MQ=255) aaaCTTACGCAGCGTGGAGATAAACGCTTCCGAAGCACCTTTTGGCGGACGCGGACGGCCACCATAAAc > 1:568832/1‑69 (MQ=255) aaaCTTACGCAGCGTGGAGATAAACGCTTCCGAAGCACCTTTTGGCGGACGCGGACGGCCACCATAAAc > 1:58266/1‑69 (MQ=255) cTTACGCAGCGTGGAGATAAATGCTTCCGAAGCACCTTTTGGCGGACGCGGACGGCCACCATAAACCgg < 1:1571675/69‑1 (MQ=255) cTTACGCAGCGTGGAGATAAATGCTTCCGAAGCACCTTTTGGCGGACGCGGACGGCCACCATAAACCgg < 1:2484161/69‑1 (MQ=255) cTTACGCAGCGTGGAGATAAATGCTTCCGAAGCACCTTTTGGCGGACGCGGACGGCCACCATAAACCgg < 1:1492856/69‑1 (MQ=255) cTTACGCAGCGTGGAGATAAATGCTTCCGAAGCACCTTTTGGCGGACGCGGACGGCCACCATAAACCgg < 1:952107/69‑1 (MQ=255) cTTACGCAGCGTGGAGATAAATGCTTCCGAAGCACCTTTTGGCGGACGCGGACGGCCACCATAAACCgg > 1:417694/1‑69 (MQ=255) gCAGCGTGGAGATAAATGCTTCCGAAGCACCTTTTGGCGGACGCGGACGGCCACCATAAACCGGATCg < 1:350781/68‑1 (MQ=255) gCAGCGTGGAGATAAATGCTTCCGAAGCACCTTTTGGCGGACGCGGACGGCCACCATAAACCGGATCg < 1:368257/68‑1 (MQ=255) gCAGCGTGGAGATAAATGCTTCCGAAGCACCTTTTGGCGGACGCGGACGGCCACCATAAACCGGATCg < 1:2549569/68‑1 (MQ=255) gCAGCGTGGAGATAAATGCTTCCGAAGCACCTTTTGGCGGACGCGGACGGCCACCATAAACCGGATCg < 1:2049331/68‑1 (MQ=255) gCAGCGTGGAGATAAATGCTTCCGAAGCACCTTTTGGCGGACGCGGACGGCCACCATAAACCGGATCg < 1:2142536/68‑1 (MQ=255) gCAGCGTGGAGATAAATGCTTCCGAAGCACCTTTTGGCGGACGCGGACGGCCACCATAAACCGGATCg < 1:2498170/68‑1 (MQ=255) gCAGCGTGGAGATAAATGCTTCCGAAGCACCTTTTGGCGGACGCGGACGGCCACCATAAACCGGATCg < 1:2626500/68‑1 (MQ=255) cAGCGTGGAGATAAATGCTTCCGAAGCACCTTTTGGCGGACGCGGACGGCCACCATAAACCGGATCg < 1:2029870/67‑1 (MQ=255) cAGCGTGGAGATAAACGCTTCCGAAGCACCTTTTGGTGGACGCGGACGGCCACCATAAACCGGATCGcc < 1:312718/69‑1 (MQ=255) cAGCGTGGAGATAAACGCTTCCGAAGCACCTTTTGGTGGACGCGGACGGCCACCATAAACCGGATCGcc < 1:896948/69‑1 (MQ=255) cAGCGTGGAGATAAACGCTTCCGAAGCACCTTTTGGTGGACGCGGACGGCCACCATAAACCGGATCGcc < 1:2931052/69‑1 (MQ=255) cAGCGTGGAGATAAACGCTTCCGAAGCACCTTTTGGTGGACGCAGACGGCCACCATAAACCGGATCGcc < 1:2797685/69‑1 (MQ=255) aGCGTGGAGATAAACGCTTCCGAAGCACCTTTTGGTGGACGCGGACGGCCACCATAAACCGGATCGcc < 1:884003/68‑1 (MQ=255) aGCGTGGAGATAAACGCTTCCGAAGCACCTTTTGGTGGACGCGGACGGCCACCATAAACCGGATCGcc < 1:1775610/68‑1 (MQ=255) gagaTAAATGCTTCCGAAGCACCTTTTGGCGGACGCGGACGGCCACCATAAACCgg > 1:3150713/1‑56 (MQ=255) gagaTAAATGCTTCCGAAGCACCTTTTGGCGGACGCGGACGGCCACCATAAACCgg > 1:224301/1‑56 (MQ=255) aaaTGCTTCCGAAGCACCTTTTGGCGGACGCGGACGGCCACCATAAACCGGATCGc > 1:1596428/1‑56 (MQ=255) aaaTGCTTCCGAAGCACCTTTTGGCGGACGCGGACGGCCACCATAAACCGGATCGc > 1:928803/1‑56 (MQ=255) aaaTGCTTCCGAAGCACCTTTTGGCGGACGCGGACGGCCACCATAAACCGGATCGc > 1:1253057/1‑56 (MQ=255) aaaTGCTTCCGAAGCACCTTTTGGCGGACGCGGACGGCCACCATAAACCGGATCGc > 1:3111967/1‑56 (MQ=255) aaaTGCTTCCGAAGCACCTTTTGGCGGACGCGGACGGCCACCATAAACCGGATCGCCCACCAGCGGATg < 1:1649592/69‑1 (MQ=255) aaaTGCTTCCGAAGCACCTTTTGGCGGACGCGGACGGCCACCATAAACCGGATCGCCCACCAGCGGATg > 1:741887/1‑69 (MQ=255) aaaTGCTTCCGAAGCACCTTTTGGCGGACGCGGACGGCCACCATAAACCGGATCGCCCACCAGCGGATg > 1:634153/1‑69 (MQ=255) aaaTGCTTCCGAAGCACCTTTTGGCGGACGCGGACGGCCACCATAAACCGGATCGCCCACCAGCGGATg > 1:1658661/1‑69 (MQ=255) aaaTGCTTCCGAAGCACCTTTTGGCGGACGCGGACGGCCACCATAAACCGGATCGCCCACCAGCGGATg < 1:95658/69‑1 (MQ=255) aaaTGCTTCCGAAGCACCTTTTGGCGGACGCGGACGGCCACCATAAACCGGATCGCCCACCAGCGGATg > 1:1894020/1‑69 (MQ=255) aaaTGCTTCCGAAGCACCTTTTGGCGGACGCGGACGGCCACCATAAACCGGATCGCCCACCAGCGGATg < 1:470617/69‑1 (MQ=255) aaaTGCTTCCGAAGCACCTTTTGGCGGACGCGGACGGCCACCATAAACCGGATCGCCCACCAGCGGATg > 1:2566089/1‑69 (MQ=255) aaaTGCTTCCGAAGCACCTTTTGGCGGACGCGGACGGCCACCATAAACCGGATCGCCCACCAGCGGATg > 1:2940314/1‑69 (MQ=255) aaaTGCTTCCGAAGCACCTTTTGGCGGACGCGGACGGCCACAATAAACCGGATCGCCCACCAGCGGATg < 1:3151328/69‑1 (MQ=255) aaaTGCTTCCGAAGCACCTTTTGGCGGACACGGACGGCCACCATAAACCGGATCGCCCACCAGCGGATg > 1:1452172/1‑69 (MQ=255) aaTGCTTCCGAAGCACCTTTTGGCGGACGCGGACGGCCACCATAAACCGGATCGCCCACCAGCGGATg < 1:2669353/68‑1 (MQ=255) aaTGCTTCCGAAGCACCTTTTGGCGGACGCGGACGGCCACCATAAACCGGATCGCCCACCAGCGGATg < 1:2980562/68‑1 (MQ=255) aaTGCTTCCGAAGCACCTTTTGGCGGACGCGGACGGCCACCATAAACCGGATCGCCCACCAGCGGATGa < 1:2263429/69‑1 (MQ=255) aaTGCTTCCGAAGCACCTTTTGGCGGACGCGGACGGCCACCATAAACCGGATCGCCCACCAGCGGATGa < 1:249417/69‑1 (MQ=255) aaTGCTTCCGAAGCACCTTTTGGCGGACGCGGACGGCCACCATAAACCGGATCGCCCACCAGCGGATGa < 1:3346905/69‑1 (MQ=255) aaTGCTTCCGAAGCACCTTTTGGCGGACGCGGACGGCCACCATAAACCGGATCGCCCACCAGCGGATGa < 1:2784975/69‑1 (MQ=255) aaTGCTTCCGAAGCACCTTTTGGCGGACGCGGACGGCCACCATAAACCGGATCGCCCACCAGCGGATGa < 1:941972/69‑1 (MQ=255) aTGCTTCCGAAGCACCTTTTGGCGGACGCGGACGGCCACCATAAACCGGATCGCCCACCAGCGGATGa < 1:907053/68‑1 (MQ=255) | ATAAAGACGCAGCATGGTTGCATGTAGCGCCTGGCGGTCAAACTTACGCAGCGTGGAGATAAATGCTTCCGAAGCACCTTTTGGCGGACGCGGACGGCCACCATAAACCGGATCGCCCACCAGCGGATGA > minE/1767837‑1767966 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |