| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,768,173 | C→T | 65.9% | A175A (GCG→GCA) | rluD ← | 23S rRNA pseudouridine synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,768,173 | 0 | C | T | 65.9% | 60.0 / 47.3 | 85 | A175A (GCG→GCA) | rluD | 23S rRNA pseudouridine synthase |
| Reads supporting (aligned to +/- strand): ref base C (14/15); new base T (25/31); total (39/46) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.20e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01 | |||||||||||
TGGGTACGTTTGGTCGGGTGGCGACTGATTGGCTCGTCCACCGTGCCACCTGCGGTCATATGACCAATCGCCACCGCTTCATACTCACGAGTAATTTCACGCCGTTGCAAAGATTCGACTAAACGCGT > minE/1768105‑1768232 | tGGGTACGTTTGGTCGGGTGGCGACTGATTGGCTCGTCCACCGTGCCACCTGCGGTCATATGACCAATc > 1:1287771/1‑69 (MQ=255)tGGGTACGTTTGGTCGGGTGGCGACTGATTGGCTCGTCCACCGTGCCACCTGCGGTCATATGACCAATc > 1:629923/1‑69 (MQ=255)tGGGTACGTTTGGTCGGGTGGCGACTGATTGGCTCGTCCACCGTGCCACCTGCGGTCATATGACCAATc > 1:61062/1‑69 (MQ=255) ggTACGTTTGGTCGGGTGGCGACTGATTGGCTCGTCCACCGTGCCACCTGCGGTCATATGACCAATCGc < 1:819695/69‑1 (MQ=255) ggTACGTTTGGTCGGGTGGCGACTGATTGGCTCGTCCACCGTGCCACCTGCGGTCATATGACCAATCGc < 1:23200/69‑1 (MQ=255) tACGTTTGGTCGGGTGGCGATTGATTGGCTCGTCCACCGTGCCACCTGCGGTCATATGACCAATCGCCa > 1:2790702/1‑69 (MQ=255) tACGTTTGGTCGGGTGGCGACTGATTGGCTCGTCCACCGTGCCACCTGCGGTCATATGCCCAATCGCCa > 1:1847066/1‑69 (MQ=255) tACGTTTGGTCGGGTGGCGACTGATTGGCTCGTCCACCGTGCCACCTGCGGTCATATGACCAATCGCCa > 1:2144404/1‑69 (MQ=255) tACGTTTGGTCGGGTGGCGACTGATTGGCTCGTCCACCGTGCCACCTGCGGTCATATGACCAATCGCCa > 1:1979440/1‑69 (MQ=255) gCCACCTGCGGTCATATGACCAATCGCCACCGCTTCATACTCACGAGTAATTTCACGCCGTTGCAAAGa < 1:2498227/69‑1 (MQ=255) gCCACCTGCGGTCATATGACCAATCGCCACCGCTTCATACTCACGAGTAATTTCACGCCGTTGCAAAGa < 1:2834303/69‑1 (MQ=255) gCCACCTGCGGTCATATGACCAATCGCCACCGCTTCATACTCACGAGTAATTTCACGCCGTTGCAAAGa < 1:3259631/69‑1 (MQ=255) gCCACCTGCGGTCATATGACCAATCGCCACCGCTTCATACTCACGAGTAATTTCACGCCGTTGCAAAGa < 1:1003238/69‑1 (MQ=255) ccACCTGCGGTCATATGACCAATCGCCACCGCTTCATACTCACGAGTAATTTCACGCCGTTGCAAAGa < 1:1227179/68‑1 (MQ=255) ccagCGGTCATATGACCAATTGCCACCGCTTCATACTCACGAGTAATTTCACGCCGTTGCAAAGCTTc > 1:3306862/4‑68 (MQ=255) ccagCGGTCATATGACCAATTGCCACCGCTTCATACTCACGAGTAATTTCACGCCGTTGCAAAGATTc > 1:3379440/4‑68 (MQ=255) ccagCGGTCATATGACCAATTGCCACCGCTTCATACTCACGAGTAATTTCACGCCGTTGCAAAGATTc > 1:3424988/4‑68 (MQ=255) ccagCGGTCATATGACCAATTGCCACCGCTTCATACTCACGAGTAATTTCACGCCGTTGCAAAGATTc > 1:301875/4‑68 (MQ=255) ccagCGGTCATATGACCAATTGCCACCGCTTCATACTCACGAGTAATTTCACGCCGTTGCAAAGATTc > 1:2243943/4‑68 (MQ=255) ccagCGGTCATATGACCAATTGCCACCGCTTCATACTCACGAGTAATTTCACGCCGTTGCAAAGATTc > 1:115186/4‑68 (MQ=255) ccagCGGTCATATGACCAATTGCCACCGCTTCATACTCACGAGTAATTTCACGCCGTTGCAAAGATTc > 1:793645/4‑68 (MQ=255) ccagCGGTCATATGACCAATTGCCACCGCTTCATACTCACGAGTAATTTCACGCCGTTGCAAAGATTCg > 1:2389606/4‑69 (MQ=255) ccagCGGTCATATGACCAATTGCCACCGCTTCATACTCACGAGTAATTTCACGCCGTTGCAAAGATTCg > 1:2274237/4‑69 (MQ=255) ccagCGGTCATATGACCAATTGCCACCGCTTCATACTCACGAGTAATTTCACGCCGTTGCAAAGATTCg > 1:2342248/4‑69 (MQ=255) ccagCGGTCATATGACCAATTGCCACCGCTTCATACTCACGAGTAATTTCACGCCGTTGCAAAGATTCg > 1:2231495/4‑69 (MQ=255) ccagCGGTCATATGACCAATTGCCACCGCTTCATACTCACGAGTAATTTCACGCCGTTGCAAAGATTCg > 1:2482267/4‑69 (MQ=255) ccagCGGTCATATGACCAATTGCCACCGCTTCATACTCACGAGTAATTTCACGCCGTTGCAAAGATTCg > 1:2487376/4‑69 (MQ=255) ccagCGGTCATATGACCAATTGCCACCGCTTCATACTCACGAGTAATTTCACGCCGTTGCAAAGATTCg > 1:2695884/4‑69 (MQ=255) ccagCGGTCATATGACCAATTGCCACCGCTTCATACTCACGAGTAATTTCACGCCGTTGCAAAGATTCg > 1:2943924/4‑69 (MQ=255) ccagCGGTCATATGACCAATTGCCACCGCTTCATACTCACGAGTAATTTCACGCCGTTGCAAAGATTCg > 1:3191238/4‑69 (MQ=255) ccagCGGTCATATGACCAATTGCCACCGCTTCATACTCACGAGTAATTTCACGCCGTTGCAAAGATTCg > 1:3329477/4‑69 (MQ=255) ccagCGGTCATATGACCAATTGCCACCGCTTCATACTCACGAGTAATTTCACGCCGTTGCAAAGATTCg > 1:653778/4‑69 (MQ=255) ccagCGGTCATATGACCAATTGCCACCGCTTCATACTCACGAGTAATTTCACGCCGTTGCAAAGATTCg > 1:781877/4‑69 (MQ=255) ccagCGGTCATATGACCAATTGCCACCGCTTCATACTCACGAGTAATTTCACGCCGTTGCAAAGATTCg > 1:971338/4‑69 (MQ=255) ccagCGGTCATATGACCAATTGCCACCGCTTCATACTCACGAGTAATTTCACGCCGTTGCAAAGATTCg > 1:1107087/4‑69 (MQ=255) ccagCGGTCATATGACCAATTGCCACCGCTTCATACTCACGAGTAATTTCACGCCGTTGCAAAGATTCg > 1:1474964/4‑69 (MQ=255) ccagCGGTCATATGACCAATTGCCACCGCTTCATACTCACGAGTAATTTCACGCCGTTGCAAAGATTCg > 1:1311547/4‑69 (MQ=255) ccagCGGTCATATGACCAATTGCCACCGCTTCATACTCACGAGTAATTTCACGCCGTTGCAAAGATTCg > 1:2076159/4‑69 (MQ=255) ccagCGGTCATATGACCAATTGCCACCGCTTCATACTCACGAGTAATTTCAAGCCGTTGCAAAGATTc > 1:147322/4‑68 (MQ=255) ccTGCGGTCATATGACCAATCGCCACCGCTTCATACTCACGAGTAATTTCACGCCGTTGCAAAGATTCg < 1:2140358/69‑1 (MQ=255) ccTGCGGTCATATGACCAATCGCCACCGCTTCATACTCACGAGTAATTTCACGCCGTTGCAAAGATTCg < 1:2090995/69‑1 (MQ=255) ccTGCGGTCATATGACCAATCGCCACCGCTTCATACTCACGAGTAATTTCACGCCGTTGCAAAGATTCg < 1:1893371/69‑1 (MQ=255) ccTGCGGTCATATGACCAATCGCCACCGCTTCATACTCACGAGTAATTTCACGCCGTTGCAAAGATTCg < 1:1886796/69‑1 (MQ=255) ccTGCGGTCATATGACCAATCGCCACCGCTTCATACTCACGAGTAATTTCACGCCGTTGCAAAGATTCg < 1:1215121/69‑1 (MQ=255) ccTGCGGTCATATGACCAATCGCCACCGCTTCATACTCACGAGTAATTTCACGCCGTTGCAAAGATTCg < 1:6156/69‑1 (MQ=255) ccTGCGGTCATATGACCAATCGCCACCGCTTCATACTCACGAGTAATTTCACGCCGTTGCAAAGATTCg < 1:2587551/69‑1 (MQ=255) ccTGCGGTCATATGACCAATCGCCACCGCTTCATACTCACGAGTAATTTCACGCCGTTGCAAAGATTCg < 1:1354248/69‑1 (MQ=255) cATATGACCAATCGCCACCGCTTCATACTCACGAGTAATTTCACGCCGTTGCAAAGATTCGACTAAAcg > 1:1531778/1‑69 (MQ=255) cATATGACCAATCGCCACCGCTTCATACTCACGAGTAATTTCACGCCGTTGCAAAGATTCGACTAAAcg > 1:39991/1‑69 (MQ=255) atatGACCAATCGCCACCGCTTCATACTCACGAGTAATTTCACGCCGTTGCAAAGATTCGACTAAAcgc > 1:2938156/1‑69 (MQ=255) atatGACCAATCGCCACCGCTTCATACTCACGAGTAATTTCACGCCGTTGCAAAGATTCGACTAAAcgc > 1:1511316/1‑69 (MQ=255) atatGACCAATCGCCACCGCTTCATACTCACGAGTAATTTCACGCCGTTGCAAAGATTCGACTAAAcgc > 1:1436044/1‑69 (MQ=255) atatGACCAATCGCCACCGCTTCATACTCACGAGTAATTTCACGCCGTTGCAAAGATTCGACTAAAcgc > 1:2341522/1‑69 (MQ=255) atGACCAATTGCCACCGCTTCATACTCACGAGTAATTTCACGCCGTTGCAAAGATTCGACTAAACTCGt < 1:1149753/69‑1 (MQ=255) atGACCAATTGCCACCGCTTCATACTCACGAGTAATTTCACGCCGTTGCAAAGATTCGACTAAACGCGt < 1:473708/69‑1 (MQ=255) atGACCAATTGCCACCGCTTCATACTCACGAGTAATTTCACGCCGTTGCAAAGATTCGACTAAACGCGt < 1:2217585/69‑1 (MQ=255) atGACCAATTGCCACCGCTTCATACTCACGAGTAATTTCACGCCGTTGCAAAGATTCGACTAAACGCGt < 1:1142126/69‑1 (MQ=255) atGACCAATTGCCACCGCTTCATACTCACGAGTAATTTCACGCCGTTGCAAAGATTCGACTAAACGCGt < 1:3158584/69‑1 (MQ=255) atGACCAATTGCCACCGCTTCATACTCACGAGTAATTTCACGCCGTTGCAAAGATTCGACTAAACGCGt < 1:1720649/69‑1 (MQ=255) atGACCAATTGCCACCGCTTCATACTCACGAGTAATTTCACGCCGTTGCAAAGATTCGACTAAACGCGt < 1:2900512/69‑1 (MQ=255) atGACCAATTGCCACCGCTTCATACTCACGAGTAATTTCACGCCGTTGCAAAGATTCGACTAAACGCGt < 1:2179505/69‑1 (MQ=255) atGACCAATTGCCACCGCTTCATACTCACGAGTAATTTCACGCCGTTGCAAAGATTCGACTAAACGCGt < 1:1738274/69‑1 (MQ=255) atGACCAATTGCCACCGCTTCATACTCACGAGTAATTTCACGCCGTTGCAAAGATTCGACTAAACGCGt < 1:1731050/69‑1 (MQ=255) atGACCAATTGCCACCGCTTCATACTCACGAGTAATTTCACGCCGTTGCAAAGATTCGACTAAACGCGt < 1:275851/69‑1 (MQ=255) atGACCAATTGCCACCGCTTCATACTCACGAGTAATTTCACGCCGTTGCAAAGATTCGACTAAACGCGt < 1:2761122/69‑1 (MQ=255) atGACCAATCGCCACCGCTTCATACTCACGAGTAATTTCACGCCGTTGCAAAGATTCGACTAAACGCGt > 1:2899021/1‑69 (MQ=255) atGACCAATCGCCACCGCTTCATACTCACGAGTAATTTCACGCCGTTGCAAAGATTCGACTAAACGCGt > 1:2632805/1‑69 (MQ=255) atGACCAATCGCCACCGCTTCATACTCACGAGTAATTTCACGCCGTTGCAAAGATTCGACTAAACGCGt > 1:2767607/1‑69 (MQ=255) atGACCAATCGCCACCGCTTCATACTCACGAGTAATTTCACGCCGTTGCAAAGATTCGACTAAACGCGt > 1:151610/1‑69 (MQ=255) tGACCAATTGCCACCGCTTCATACTCACGAGTAATTTCACGCCGTTGCAAAGATTCGACTAAACGCGt < 1:2702764/68‑1 (MQ=255) tGACCAATTGCCACCGCTTCATACTCACGAGTAATTTCACGCCGTTGCAAAGATTCGACTAAACGCGt < 1:236022/68‑1 (MQ=255) tGACCAATTGCCACCGCTTCATACTCACGAGTAATTTCACGCCGTTGCAAAGATTCGACTAAACGCGt < 1:373748/68‑1 (MQ=255) tGACCAATTGCCACCGCTTCATACTCACGAGTAATTTCACGCCGTTGCAAAGATTCGACTAAACGCGt < 1:2660945/68‑1 (MQ=255) gACCAATTGCCACCGCTTCATACTCACGAGTAATTTCACGCCGTTGCAAAGATTCGACTAAACGCGt < 1:545772/67‑1 (MQ=255) gACCAATTGCCACCGCTTCATACTCACGAGTAATTTCACGCCGTTGCAAAGATTCGACTAAACGCGt < 1:3063353/67‑1 (MQ=255) aCCAATTGCCACCGCTTCATACTCACGAGTAATTTc < 1:1727285/36‑1 (MQ=255) aCCAATTGCCACCGCTTCATACTCACGAGTAATTTc < 1:480809/36‑1 (MQ=255) aCCAATTGCCACCGCTTCATACTCACGAGTAATTTc < 1:487705/36‑1 (MQ=255) aCCAATTGCCACCGCTTCATACTCACGAGTAATTTc < 1:3287145/36‑1 (MQ=255) aCCAATTGCCACCGCTTCATACTCACGAGTAATTTc < 1:1760825/36‑1 (MQ=255) aCCAATTGCCACCGCTTCATACTCACGAGTAATTTc < 1:1819499/36‑1 (MQ=255) aCCAATTGCCACCGCTTCATACTCACGAGTAATTTc < 1:1294787/36‑1 (MQ=255) aCCAATTGCCACCGCTTCATACTCACGAGTAATTTc < 1:182322/36‑1 (MQ=255) aCCAATTGCCACCGCTTCATACTCACGAGTAATTTc < 1:1175507/36‑1 (MQ=255) aCCAATTGCCACCGCTTCATACTCACGAGTAATTTc < 1:2157350/36‑1 (MQ=255) aCCAATTGCCACCGCTTCATACTCACGAGTAATTTc < 1:897459/36‑1 (MQ=255) aCCAATTGCCACCGCTTCATACTCACGAGTAATTTc < 1:94321/36‑1 (MQ=255) aCCAATTGCCACCGCTTCATACTCACGAGTAATTTc < 1:2982182/36‑1 (MQ=255) | TGGGTACGTTTGGTCGGGTGGCGACTGATTGGCTCGTCCACCGTGCCACCTGCGGTCATATGACCAATCGCCACCGCTTCATACTCACGAGTAATTTCACGCCGTTGCAAAGATTCGACTAAACGCGT > minE/1768105‑1768232 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |