| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,771,423 | C→T | 34.9% | R331R (CGC→CGT) | pheA → | fused chorismate mutase P and prephenate dehydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,771,423 | 0 | C | T | 34.9% | 77.4 / 58.1 | 103 | R331R (CGC→CGT) | pheA | fused chorismate mutase P and prephenate dehydratase |
| Reads supporting (aligned to +/- strand): ref base C (50/17); new base T (18/18); total (68/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.64e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01 | |||||||||||
Only 100 of 103 total aligned reads displayed.
GCGCTGGTTGAAGCGTTGCTGGTACTGCGCAACCACAATCTGATTATGACCCGTCTGGAATCACGCCCGATTCACGGTAATCCATGGGAAGAGATGTTCTATCTGGATATTCAGGCCAATCTTGAATCAG > minE/1771358‑1771487 | tcacTGGTTGAAGCGTTGCTGGTACTGCGCAATCACAGTCTGATTATGACCCGTCTGGAATCACGTCCg < 1:2597724/66‑1 (MQ=255)gctcTGGTTGAAGCGTTGCTGGTACTGCGCAATCACAGTCTGATTATGACCCGTCTGGAATCACGTCCg < 1:615981/66‑1 (MQ=255)gcacTGGTTGAAGCGTTGCTGGTACTGCGCAATCACAGTCTGATTATGACCCGTCTGGAATCACGTCCg < 1:411001/66‑1 (MQ=255)gcacTGGTTGAAGCGTTGCTGGTACTGCGCAATCACAGTCTGATTATGACCCGTCTGGAATCACGTCCg < 1:3071728/66‑1 (MQ=255)gcacTGGTTGAAGCGTTGCTGGTACTGCGCAATCACAGTCTGATTATGACCCGTCTGGAATCACGTCCg < 1:156580/66‑1 (MQ=255)gcacTGGTTGAAGCGTTGCTGGTACTGCGCAATCACAGTCTGATTATGACCCGTCTGGAATCACGTCCg < 1:3224115/66‑1 (MQ=255)gcacTGGTTGAAGCGTTGCTGGTACTGCGCAATCACAGTCTGATTATGACCCGTCTGGAATCACGTCCg < 1:1385454/66‑1 (MQ=255)gcacTGGTTGAAGCGTTGCTGGTACTGCGCAATCACAGTCTGATTATGACCCGTCTGGAATCACGTCCg < 1:1380822/66‑1 (MQ=255)gcacTGGTTGAAGCGTTGCTGGTACTGCGCAATCACAGTCTGATTATGACCCGTCTGGAATCACGTCCg < 1:3067664/66‑1 (MQ=255)gcacTGGTTGAAGCGTTGCTGGTACTGCGCAATCACAGTCTGATTATGACCCGTCTGGAATCACGTCCg < 1:1304919/66‑1 (MQ=255)gcacTGGTTGAAGCGTTGCTGGTACTGCGCAATCACAGTCTGATTATGACCCGTCTGGAATCACGTCCg < 1:1665530/66‑1 (MQ=255)gcacTGGTTGAAGCGTTGCTGGTACTGCGCAATCACAGTCTGATTATGACCCGTCTGGAATCACGTCCg < 1:1686193/66‑1 (MQ=255)gcacTGGTTGAAGCGTTGCTGGTACTGCGCAATCACAGTCTGATTATGACCCGTCTGGAATCACGTCCg < 1:2699757/66‑1 (MQ=255)gcacTGGTTGAAGCGTTGCTGGTACTGCGCAATCACAGTCTGATTATGACCCGTCTGGAATCACGTCCg < 1:2456826/66‑1 (MQ=255)gcacTGGTTGAAGCGTTGCTGGTACTGCGCAATCACAGTCTGATTATGACCCGTCTGGAATCACGTCCg < 1:842044/66‑1 (MQ=255)gcacTGGTTGAAGCGTTGCTGGTACTGCGCAATCACAGTCTGATTATGACCCGTCTGGAATCACGTCCg < 1:914720/66‑1 (MQ=255)gcacTGGTTGAAGCGTTGCTGGTACTGCGCAATCACAGTCTGATTATGACCCGTCTGGAATCACGTCCg < 1:2298290/66‑1 (MQ=255) gAAGCGTTGCTGGTACTGCGCAACCACAATCTGATTATGACCCGTCTGGAATCACGCCCGa > 1:1525662/1‑61 (MQ=255) gAAGCGTTGCTGGTACTGCGCAACCACAATCTGATTATGACCCGTCTGGAATCACGCCCGATTCACgg > 1:848213/1‑68 (MQ=255) gAAGCGTTGCTGGTACTGCGCAACCACAATCTGATTATGACCCGTCTGGAATCACGCCCGATTCACGGt > 1:1013991/1‑69 (MQ=255) gAAGCGTTGCTGGTACTGCGCAACCACAATCTGATTATGACCCGTCTGGAATCACGCCCGATTCACGGt > 1:2812022/1‑69 (MQ=255) gAAGCGTTGCTGGTACTGCGCAACCACAATCTGATTATGACCCGTCTGGAATCACGCCCGATTCACGGt > 1:2448156/1‑69 (MQ=255) gAAGCGTTGCTGGTACTGCGCAACCACAATCTGATTATGACCCGTCTGGAATCACGCCCGATTCACGGt > 1:1607150/1‑69 (MQ=255) gAAGCGTTGCTGGTACTGCGCAACCACAATCTGATTATGACCCGTCTGGAATCACGCCCGATTCACGGt > 1:3412327/1‑69 (MQ=255) gAAGCGTTGCTGGTACTGCGCAACCACAATCTGATTATGACCCGTCTGGAATCACGCCCGATTCACGGt > 1:762637/1‑69 (MQ=255) cGTTGCTGGTACTGCGCAACCACAATCTGATTATGACCCGTCTGGAATCACGCCCGATTCACGGTAATc > 1:153395/1‑69 (MQ=255) cGTTGCTGGTACTGCGCAACCACAATCTGATTATGACCCGTCTGGAATCACGCCCGATTCACGGTAATc > 1:3184782/1‑69 (MQ=255) cGTTGCTGGTACTGCGCAACCACAATCTGATTATGACCCGTCTGGAATCACGCCCGATTCACGGTAATc > 1:1677394/1‑69 (MQ=255) cGTTGCTGGTACTGCGCAACCACAATCTGATTATGACCCGTCTGGAATCACGCCCGATTCACGGTAATc < 1:505691/69‑1 (MQ=255) cGTTGCTGGTACTGCGCAACCACAATCTGATTATGACCCGTCTGGAATCACGCCCGATTCACGGTAATc < 1:717695/69‑1 (MQ=255) cAACCACAATCTGATTATGACCCGTCTGGAATCACGCCCGATTCACGGTAATCCATGGGAagag > 1:576969/1‑64 (MQ=255) cAACCACAATCTGATTATGACCCGTCTGGAATCACGCCCGATTCACGGTAATCCATGGGAAgaga > 1:394118/1‑65 (MQ=255) cAACCACAATCTGATTATGACCCGTCTGGAATCACGCCCGATTCACGGTAATCCATGGGAAgaga > 1:3002635/1‑65 (MQ=255) cAACCACAATCTGATTATGACCCGTCTGGAATCACGCCCGATTCACGGTAATCCATGGGAAgaga > 1:39752/1‑65 (MQ=255) cAACCACAATCTGATTATGACCCGTCTGGAATCACGCCCGATTCACGGTAATCCATGGGAAgaga > 1:50201/1‑65 (MQ=255) cAACCACAATCTGATTATGACCCGTCTGGAATCACGCCCGATTCACGGTAATCCATGGGAAgaga > 1:2715972/1‑65 (MQ=255) cAACCACAATCTGATTATGACCCGTCTGGAATCACGCCCGATTCACGGTAATCCATGGGAAgaga > 1:2428270/1‑65 (MQ=255) cAACCACAATCTGATTATGACCCGTCTGGAATCACGCCCGATTCACGGTAATCCATGGGAAgaga > 1:915404/1‑65 (MQ=255) cAACCACAATCTGATTATGACCCGTCTGGAATCACGCCCGATTCACGGTAATCCATGGGAAgaga > 1:1239461/1‑65 (MQ=255) cAACCACAATCTGATTATGACCCGTCTGGAATCACGCCCGATTCACGGTAATCCATGGGAAgaga > 1:1823304/1‑65 (MQ=255) aaCCACAATCTGATTATGACCCGTCTGGAATCACGCCCGATTCACGGTAATCCATGGGAAGAGATGTTc < 1:2934491/69‑1 (MQ=255) aCCACAATCTGATTATGACCCGTCTGGAATCACGCCCGATTCACGGTAATCCATGGGAAGAGATGTTc < 1:2933602/68‑1 (MQ=255) agtCTGATTATGACCCGTCTGGAATCACGTCCGATTCACGGTAATCCGTGGGAAGAGAt > 1:661114/3‑59 (MQ=255) agtCTGATTATGACCCGTCTGGAATCACGTCCGATTCACGGTAATCCGTGGGAAGAGATg > 1:2386185/3‑60 (MQ=255) agtCTGATTATGACCCGTCTGGAATCACGTCCGATTCACGGTAATCCGTGGGAAGAGATGTTTTATCTg > 1:1763984/3‑69 (MQ=255) agtCTGATTATGACCCGTCTGGAATCACGTCCGATTCACGGTAATCCGTGGGAAGAGATGTTTTATCTg > 1:3416111/3‑69 (MQ=255) agtCTGATTATGACCCGTCTGGAATCACGTCCGATTCACGGTAATCCGTGGGAAGAGATGTTTTATCTg > 1:1420177/3‑69 (MQ=255) agtCTGATTATGACCCGTCTGGAATCACGTCCGATTCACGGTAATCCGTGGGAAGAGATGTTTTATCTg > 1:3106920/3‑69 (MQ=255) agtCTGATTATGACCCGTCTGGAATCACGTCCGATTCACGGTAATCCGTGGGAAGAGATGTTTTATCTg > 1:155004/3‑69 (MQ=255) agtCTGATTATGACCCGTCTGGAATCACGTCCGATTCACGGTAATCCGTGGGAAGAGATGTTTTATCTg > 1:3018866/3‑69 (MQ=255) agtCTGATTATGACCCGTCTGGAATCACGTCCGATTCACGGTAATCCGTGGGAAGAGATGTTTTATCTg > 1:273413/3‑69 (MQ=255) agtCTGATTATGACCCGTCTGGAATCACGTCCGATTCACGGTAATCCGTGGGAAGAGATGTTTTATCTg > 1:2559102/3‑69 (MQ=255) agtCTGATTATGACCCGTCTGGAATCACGTCCGATTCACGGTAATCCGTGGGAAGAGATGTTTTATCTg > 1:2513979/3‑69 (MQ=255) agtCTGATTATGACCCGTCTGGAATCACGTCCGATTCACGGTAATCCGTGGGAAGAGATGTTTTATCTg > 1:2161984/3‑69 (MQ=255) agtCTGATTATGACCCGTCTGGAATCACGTCCGATTCACGGTAATCCGTGGGAAGAGATGTTTTATCTg > 1:2228089/3‑69 (MQ=255) agtCTGATTATGACCCGTCTGGAATCACGTCCGATTCACGGTAATCCGTGGGAAGAGATGTTTTATCTg > 1:2272467/3‑69 (MQ=255) agtCTGATTATGACCCGTCTGGAATCACGTCCGATTCACGGTAATCCGTGGGAAGAGATGTTTTATCTg > 1:2405714/3‑69 (MQ=255) agtCTGATTATGACCCGTCTGGAATCACGTCCGATTCACGGTAATCCGTGGGAAGAGATGTTTTATCTg > 1:238381/3‑69 (MQ=255) agtCTGATTATGACCCGTCTGGAATCACGTCCGATTCACGGTAATCCGTGGGAAGAGATGTTTTATCTg > 1:110837/3‑69 (MQ=255) agacTGATTATGACCCGTCTGGAATCACGTCCGATTCACGGTAATCCGTGGGAAGAGATGTTTTATCt > 1:1668047/4‑68 (MQ=255) aaTCTGATTATGACCCGTCTGGAATCACGCCCGATTCACGGTAATCCATGGGAAGAGATGTTCTATCTg < 1:1120143/69‑1 (MQ=255) tGATTATGACCCGTCTGGAATCACGCCCGATTCACGGTAATCCATGGGAAGAGATGTTCTATCTGGata > 1:109321/1‑69 (MQ=255) tGATTATGACCCGTCTGGAATCACGCCCGATTCACGGTAATCCATGGGAAGAGATGTTCTATCTGGata > 1:1296199/1‑69 (MQ=255) tGATTATGACCCGTCTGGAATCACGCCCGATTCACGGTAATCCATGGGAAGAGATGTTCTATCTGGata > 1:1291761/1‑69 (MQ=255) tGATTATGACCCGTCTGGAATCACGCCCGATTCACGGTAATCCATGGGAAGAGATGTTCTATCTGGata > 1:2298148/1‑69 (MQ=255) tGATTATGACCCGTCTGGAATCACGCCCGATTCACGGTAATCCATGGGAAGAGATGTTCTATCTGGat > 1:823606/1‑68 (MQ=255) ccGTCTGGAATCACGCCCGATTCACTGTAATCCATGGGAAGAGATGTTCTATCTGGATATTCAGGCCa < 1:2924995/68‑1 (MQ=255) ccGTCTGGAATCACGCCCGATTCACGGTAATCCATGGGAAGAGATGTTCTATCTGGATATTCAGGCCa < 1:427015/68‑1 (MQ=255) ccGTCTGGAATCACGCCCGATTCACGGTAATCCATGGGAAGAGATGTTCTATCTGGATATTCAGGCCa < 1:3078393/68‑1 (MQ=255) ccGTCTGGAATCACGCCCGATTCACGGTAATCCATGGGAAGAGATGTTCTATCTGGATATTCAGGCCa < 1:1229024/68‑1 (MQ=255) ccGTCTGGAATCACGCCCGATTCACGGTAATCCATGGGAAGAGATGTTCTATCTGGATATTCAGGCCa < 1:1911425/68‑1 (MQ=255) ccGTCTGGAATCACGCCCGATTCACGGTAATCCATGGGAAGAGATGTTCTATCTGGATATTCAGGCCa < 1:777589/68‑1 (MQ=255) tGGAATCACGCCCGATTCACGGTAATCCATGGGAAGAGATGTTCTATCTGGATATTCAGGCCAATCTTg > 1:1277500/1‑69 (MQ=255) tGGAATCACGCCCGATTCACGGTAATCCATGGGAAGAGATGTTCTATCTGGATATTCAGGCCAATCTTg > 1:2726920/1‑69 (MQ=255) tGGAATCACGCCCGATTCACGGTAATCCATGGGAAGAGATGTTCTATCTGGATATTCAGGCCAATCTTg < 1:3380412/69‑1 (MQ=255) aTCACGCCCGATTCACGGTAATCCATGGGAAGAGATGTTCTATCTGGATATTCAGGCCa < 1:1066601/59‑1 (MQ=255) aTCACGCCCGATTCACGGTAATCCATGGGAAGAGATGTTCTATCTGGATATTCAGGCCAATCTTGa < 1:2691359/66‑1 (MQ=255) aTCACGCCCGATTCACGGTAATCCATGGGAAGAGATGTTCTATCTGGATATTCAGGCCAATCTTGa < 1:1171020/66‑1 (MQ=255) cACGCCCGATTCACGGTAATCCATGGGAAGAGATGTTCTATCTGGATATTCAGGCCAATCTTGaa > 1:1071069/1‑65 (MQ=255) cACGCCCGATTCACGGTAATCCATGGGAAGAGATGTTCTATCTGGATATTCAGGCCAATCTTGa > 1:178202/1‑64 (MQ=255) cACGCCCGATTCACGGTAATCCATGGGAAGAGATGTTCTATCTGGATATTCAGGCCAATCTTGAATCAg > 1:373392/1‑69 (MQ=255) cACGCCCGATTCACGGTAATCCATGGGAAGAGATGTTCTATCTGGATATTCAGGCCAATCTTGAATCAg > 1:2029387/1‑69 (MQ=255) cACGCCCGATTCACGGTAATCCATGGGAAGAGATGTTCTATCTGGATATTCAGGCCAATCTTGAATCAg > 1:190119/1‑69 (MQ=255) cACGCCCGATTCACGGTAATCCATGGGAAGAGATGTTCTATCTGGATATTCAGGCCAATCTTGAATCAg > 1:530316/1‑69 (MQ=255) cACGCCCGATTCACGGTAATCCATGGGAAGAGATGTTCTATCTGGATATTCAGGCCAATCTTGAATCAg > 1:525499/1‑69 (MQ=255) cACGCCCGATTCACGGTAATCCATGGGAAGAGATGTTCTATCTGGATATTCAGGCCAATCTTGAATCAg > 1:2863463/1‑69 (MQ=255) cACGCCCGATTCACGGTAATCCATGGGAAGAGATGTTCTATCTGGATATTCAGGCCAATCTTGAATCAg > 1:1659417/1‑69 (MQ=255) cACGCCCGATTCACGGTAATCCATGGGAAGAGATGTTCTATCTGGATATTCAGGCCAATCTTGAATCAg > 1:1290935/1‑69 (MQ=255) cACGCCCGATTCACGGTAATCCATGGGAAGAGATGTTCTATCTGGATATTCAGGCCAATCTTGAATCAg > 1:1310392/1‑69 (MQ=255) ccGCCCGATTCACGGTAATCCATGGGAAGAGATGTTCTATCTGGATATTCAGGCCAATc > 1:168399/2‑59 (MQ=255) aCGCCCGATTCACGGTAATCCATGGGAAGAGATGTTCTATCTGGATATTCAGGCCa < 1:2091866/56‑1 (MQ=255) aCGCCCGATTCACGGTAATCCATGGGAAGAGATGTTCTATCTGGATATTCAGGCCAATCTTGAAt > 1:2004182/1‑65 (MQ=255) aCGCCCGATTCACGGTAATCCATGGGAAGAGATGTTCTATCTGGATATTCAGGCCAATCTTGAATCAg > 1:540018/1‑68 (MQ=255) aCGCCCGATTCACGGTAATCCATGGGAAGAGATGTTCTATCTGGATATTCAGGCCAATCTTGAATCAg > 1:656262/1‑68 (MQ=255) aCGCCCGATTCACGGTAATCCATGGGAAGAGATGTTCTATCTGGATATTCAGGCCAATCTTGAATCAg > 1:1153363/1‑68 (MQ=255) aCGCCCGATTCACGGTAATCCATGGGAAGAGATGTTCTATCTGGATATTCAGGCCAATCTTGAATCAg > 1:2678678/1‑68 (MQ=255) aCGCCCGATTCACGGTAATCCATGGGAAGAGATGTTCTATCTGGATATTCAGGCCAATCTTGAATCAg > 1:784546/1‑68 (MQ=255) aCGCCCGATTCACGGTAATCCATGGGAAGAGATGTTCTATCTGGATATTCAGGCCAATCTTGAATCAg > 1:1481813/1‑68 (MQ=255) aCGCCCGATTCACGGTAATCCATGGGAAGAGATGTTCTATCTGGATATTCAGGCCAATCTTGAATCAg > 1:2053405/1‑68 (MQ=255) aCGCCCGATTCACGGTAATCCATGGGAAGAGATGTTCTATCTGGATATTCAGCCCAATCtt > 1:3119892/1‑61 (MQ=255) | GCGCTGGTTGAAGCGTTGCTGGTACTGCGCAACCACAATCTGATTATGACCCGTCTGGAATCACGCCCGATTCACGGTAATCCATGGGAAGAGATGTTCTATCTGGATATTCAGGCCAATCTTGAATCAG > minE/1771358‑1771487 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |