| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,772,153 | A→G | 43.8% | G201G (GGT→GGC) | tyrA ← | fused chorismate mutase T and prephenate dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,772,153 | 0 | A | G | 43.8% | 25.1 / 83.5 | 105 | G201G (GGT→GGC) | tyrA | fused chorismate mutase T and prephenate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base A (24/35); new base G (23/23); total (47/58) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.29e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01 | |||||||||||
Only 100 of 105 total aligned reads displayed.
ATGCTTCCGGTTTACGTCCATCACACCAGACCACAACTTGCTTTGCCAGGCTACCGCTGTCCGGACCGAACATCGGGTGTAGCCCCAGCACCGGACCATCATGCGCCACCAGCATGGCCTGTA > minE/1772089‑1772211 | aTGCTTCCGGCTTACGTCCATCACACCAGACCACAACTTGCTTTGCCAGGCTACCGCTGTCCGGGCCg > 1:1948344/1‑68 (MQ=255)aTGCTTCCGGCTTACGTCCATCACACCAGACCACAACTTGCTTTGCCAGGCTACCGCTGTCCGGGCCg > 1:211112/1‑68 (MQ=255)aTGCTTCCGGCTTACGTCCATCACACCAGACCACAACTTGCTTTGCCAGGCTACCGCTGTCCGGGCCg > 1:1577054/1‑68 (MQ=255)aTGCTTCCGGCTTACGTCCATCACACCAGACCACAACTTGCTTTGCCAGGCTACCGCTGTCCGGGCCg > 1:1147132/1‑68 (MQ=255)aTGCTTCCGGCTTACGTCCATCACACCAGACCACAACTTGCTTTGCCAGGCTACCGCTGTCCGGGCCg > 1:2647918/1‑68 (MQ=255)aTGCTTCCGGCTTACGTCCATCACACCAGACCACAACTTGCTTTGCCAGGCTACCGCTGTCCGGGCCg > 1:2618950/1‑68 (MQ=255)aTGCTTCCGGCTTACGTCCATCACACCAGACCACAACTTGCTTTGCCAGGCTACCGCTGTCCGGGCCg > 1:3291274/1‑68 (MQ=255)aTGCTTCCGGCTTACGTCCATCACACCAGACCACAACTTGCTTTGCCAGGCTACCGCTGTCCGGGCCg > 1:2347984/1‑68 (MQ=255)aTGCTTCCGGCTTACGTCCATCACACCAGACCACAACTTGCTTTGCCAGGCTACCGCTGTCCGGGCCg > 1:2431253/1‑68 (MQ=255)aTGCTTCCGGCTTACGTCCATCACACCAGACCACAACTTGCTTTGCCAGGCTACCGCTGTCCGGGCCg > 1:2742385/1‑68 (MQ=255)aTGCTTCCGGCTTACGTCCATCACACCAGACCACAACTTGCTTTGCCAGGCTACCGCTGTCCGGGCCg > 1:251690/1‑68 (MQ=255)aTGCTTCCGGCTTACGTCCATCACACCAGACCACAACTTGCTTTGCCAGGCTACCGCTGTCCGGGCCg > 1:1465653/1‑68 (MQ=255) tGCTTCCGGTTTACGTCCATCACACCAGACCACAACTTGCTTTGCCAGGCTACCGCTGTCCGGACCGaa > 1:656392/1‑69 (MQ=255) tGCTTCCGGTTTACGTCCATCACACCAGACCACAACTTGCTTTGCCAGGCTACCGCTGTCCGGACCGaa > 1:910954/1‑69 (MQ=255) tGCTTCCGGTTTACGTCCATCACACCAGACCACAACTTGCTTTGCCAGGCTACCGCTGTCCGGACCGaa > 1:3372704/1‑69 (MQ=255) tGCTTCCGGTTTACGTCCATCACACCAGACCACAACTTGCTTTGCCAGGCTACCGCTGTCCGGACCGaa > 1:1004708/1‑69 (MQ=255) tGCTTCCGGTTTACGTCCATCACACCAGACCACAACTTGCTTTGCCAGGCTACCGCTGTCCGGACCGaa > 1:2669944/1‑69 (MQ=255) aCGTCCATCACACCAGACCACAACTTGCTTTGCCAGGCTACCGCTGTCCGGGCCGAACa > 1:3275065/1‑59 (MQ=255) aCGTCCATCACACCAGACCACAACTTGCTTTGCCAGGCTACCGCTGTCCGGGCCGAACATCggg > 1:764173/1‑64 (MQ=255) aCGTCCATCACACCAGACCACAACTTGCTTTGCCAGGCTACCGCTGTCCGGGCCGAACATCGGgt > 1:2806379/1‑65 (MQ=255) aCGTCCATCACACCAGACCACAACTTGCTTTGCCAGGCTACCGCTGTCCGGGCCGAACATCGGgt > 1:2232815/1‑65 (MQ=255) aCGTCCATCACACCAGACCACAACTTGCTTTGCCAGGCTACCGCTGTCCGGGCCGAACATCGGgt > 1:939235/1‑65 (MQ=255) aCGTCCATCACACCAGACCACAACTTGCTTTGCCAGGCTACCGCTGTCCGGGCCGAACATCGGgt > 1:3226993/1‑65 (MQ=255) aCGTCCATCACACCAGACCACAACTTGCTTTGCCAGGCTACCGCTGTCCGGGCCGAACATCGGgt > 1:2989606/1‑65 (MQ=255) aCGTCCATCACACCAGACCACAACTTGCTTTGCCAGGCTACCGCTGTCCGGGCCGAACATCGGgt > 1:824340/1‑65 (MQ=255) aCGTCCATCACACCAGACCACAACTTGCTTTGCCAGGCTACCGCTGTCCGGGCCGAACATCGGGTGTa > 1:2271311/1‑68 (MQ=255) tctaTCACACCAGACCACAACTTGCTTTGCCAGGCTACCGCTGTCCGGACCGAACATCGGGTGTAGc > 1:2077593/4‑67 (MQ=255) tctaTCACACCAGACCACAACTTGCTTTGCCAGGCTACCGCTGTCCGGACCGAACATCGGGTGTAGc > 1:1313898/4‑67 (MQ=255) tctaTCACACCAGACCACAACTTGCTTTGCCAGGCTACCGCTGTCCGGACCGAACATCGGGTGTAGc > 1:1998716/4‑67 (MQ=255) tctaTCACACCAGACCACAACTTGCTTTGCCAGGCTACCGCTGTCCGGACCGAACATCGGGTGTAGc > 1:2576615/4‑67 (MQ=255) tctaTCACACCAGACCACAACTTGCTTTGCCAGGCTACCGCTGTCCGGACCGAACATCGGGTGTAGc > 1:1460516/4‑67 (MQ=255) tctaTCACACCAGACCACAACTTGCTTTGCCAGGCTACCGCTGTCCGGACCGAACATCGGGTGTAGc > 1:3366322/4‑67 (MQ=255) tctaTCACACCAGACCACAACTTGCTTTGCCAGGCTACCGCTGTCCGGACCGAACATCGGGTGTAGc > 1:2319222/4‑67 (MQ=255) tctaTCACACCAGACCACAACTTGCTTTGCCAGGCTACCGCTGTCCGGACCGAACATCGGGTGTAGc > 1:532095/4‑67 (MQ=255) acacCAGACCACAACTTGCTTTGCCAGGCTACCGCTGTCCGGACCGAACATCggg > 1:1194806/1‑55 (MQ=255) acacCAGACCACAACTTGCTTTGCCAGGCTACCGCTGTCCGGACCGAACATCggg > 1:924697/1‑55 (MQ=255) acacCAGACCACAACTTGCTTTGCCAGGCTACCGCTGTCCGGACCGAACATCggg > 1:2794989/1‑55 (MQ=255) acacCAGACCACAACTTGCTTTGCCAGGCTACCGCTGTCCGGACCGAACATCggg > 1:2459937/1‑55 (MQ=255) acacCAGACCACAACTTGCTTTGCCAGGCTACCGCTGTCCGGACCGAACATCggg > 1:331230/1‑55 (MQ=255) acacCAGACCACAACTTGCTTTGCCAGGCTACCGCTGTCCGGACCGAACATCggg > 1:2389688/1‑55 (MQ=255) acacCAGACCACAACTTGCTTTGCCAGGCTACCGCTGTCCGGACCGAACATCggg > 1:2383374/1‑55 (MQ=255) acacCAGACCACAACTTGCTTTGCCAGGCTACCGCTGTCCGGACCGAACATCggg > 1:2184917/1‑55 (MQ=255) acacCAGACCACAACTTGCTTTGCCAGGCTACCGCTGTCCGGACCGAACATCggg > 1:1601804/1‑55 (MQ=255) acacCAGACCACAACTTGCTTTGCCAGGCTACCGCTGTCCGGACCGAACATCggg > 1:1917000/1‑55 (MQ=255) aCCACAACTTGCTTTGCCAGGCTACCGCTGTCCGGACCGAACATCGGGTGTAGCCCCAGCACCTGACca < 1:710080/69‑1 (MQ=255) aCCACAACTTGCTTTGCCAGGCTACCGCTGTCCGGACCGAACATCGGGTGTAGCCCCAGCACCGGACca < 1:683580/69‑1 (MQ=255) aCCACAACTTGCTTTGCCAGGCTACCGCTGTCCGGACCGAACATCGGGTGTAGCCCCAGCACCGGACca < 1:552695/69‑1 (MQ=255) aCCACAACTTGCTTTGCCAGGCTACCGCTGTCCGGACCGAACATCGGGTGTAGCCCCAGCACCGGACca < 1:2688194/69‑1 (MQ=255) aCCACAACTTGCTTTGCCAGGCTACCGCTGTCCGGACCGAACATCGGGTGTAGCCCCAGCACCGGACca < 1:200725/69‑1 (MQ=255) ccACAACTTGCTTTGCCAGGCTACCGCTGTCCGGACCTAACATCGGGTGTAGCCCCAGCACCGGACcat < 1:2750167/69‑1 (MQ=255) ccACAACTTGCTTTGCCAGGCTACCGCTGTCCGGACCGAACATCGGGTGTAGCCCCAGCACCGGACcat < 1:2958695/69‑1 (MQ=255) ccACAACTTGCTTTGCCAGGCTACCGCTGTCCGGACCGAACATCGGGTGTAGCCCCAGCACCGGACcat < 1:2283866/69‑1 (MQ=255) ccACAACTTGCTTTGCCAGGCTACCGCTGTCCGGACCGAACATCGGGTGTAGCCCCAGCACCGGACcat < 1:481967/69‑1 (MQ=255) ccACAACTTGCTTTGCCAGGCTACCGCTGTCCGGACCGAACATCGGGTGTAGCCCCAGCACCGGACcat < 1:489120/69‑1 (MQ=255) ccACAACTTGCTTTGCCAGGCTACCGCTGTCCGGACCGAACATCGGGTGTAGCCCCAGCACCGGACcat < 1:2499912/69‑1 (MQ=255) ccACAACTTGCTTTGCCAGGCTACCGCTGTCCGGACCGAACATCGGGTGTAGCCCCAGCACCGGACcat < 1:544210/69‑1 (MQ=255) ccACAACTTGCTTTGCCAGGCTACCGCTGTCCGGACCGAACATCGGGTGTAGCCCCAGCACCGGACcat < 1:1858361/69‑1 (MQ=255) ccACAACTTGCTTTGCCAGGCTACCGCTGTCCGGACCGAACATCGGGTGTAGCCCCAGCACCGGACcat < 1:1556602/69‑1 (MQ=255) ccACAACTTGCTTTGCCAGGCTACCGCTGTCCGGACCGAACATCGGGTGTAGCCCCAGCACCGGACcat < 1:1538974/69‑1 (MQ=255) ccACAACTTGCTTTGCCAGGCTACCGCTGTCCGGACCGAACATCGGGTGTAGCCCCAGCACCGGACcat < 1:1051633/69‑1 (MQ=255) aaCTTGCTTTG‑CAGGCTACCGCTGTCCGGGCCGAACATCGGGTGTAACCCCAGCACCGGGCCATCGTgc < 1:1120963/69‑1 (MQ=255) aCTTGCTTTGCCAGGCTACCGCTGTCCGGGCCGAACATCGGGTGTAACCCCAGCACGGGGGCATCGTgc < 1:2938567/69‑1 (MQ=255) aCTTGCTTTGCCAGGCTACCGCTGTCCGGGCCGAACATCGGGTGTAACCCCAGCACCGGGCCATCGTgc < 1:513977/69‑1 (MQ=255) aCTTGCTTTGCCAGGCTACCGCTGTCCGGGCCGAACATCGGGTGTAACCCCAGCACCGGGCCATCGTgc < 1:2660029/69‑1 (MQ=255) aCTTGCTTTGCCAGGCTACCGCTGTCCGGGCCGAACATCGGGTGTAACCCCAGCACCGGGCCATCGTgc < 1:3316413/69‑1 (MQ=255) aCTTGCTTTGCCAGGCTACCGCTGTCCGGGCCGAACATCGGGTGTAACCCCAGCACCGGGCCATCGTgc < 1:91963/69‑1 (MQ=255) aCTTGCTTTGCCAGGCTACCGCTGTCCGGGCCGAACATCGGGTGTAACCCCAGCACCGGGCCATCGTgc < 1:3177792/69‑1 (MQ=255) aCTTGCTTTGCCAGGCTACCGCTGTCCGGGCCGAACATCGGGTGTAACCCCAGCACCGGGCCATCGTgc < 1:3113727/69‑1 (MQ=255) aCTTGCTTTGCCAGGCTACCGCTGTCCGGGCCGAACATCGGGTGTAACCCCAGCACCGGGCCATCGTgc < 1:1437926/69‑1 (MQ=255) aCTTGCTTTGCCAGGCTACCGCTGTCCGGGCCGAACATCGGGTGTAACCCCAGCACCGGGCCATCGTgc < 1:3053328/69‑1 (MQ=255) aCTTGCTTTGCCAGGCTACCGCTGTCCGGGCCGAACATCGGGTGTAACCCCAGCACCGGGCCATCGTgc < 1:2873956/69‑1 (MQ=255) aCTTGCTTTGCCAGGCTACCGCTGTCCGGGCCGAACATCGGGTGTAACCCCAGCACCGGGCCATCGTgc < 1:2871743/69‑1 (MQ=255) aCTTGCTTTGCCAGGCTACCGCTGTCCGGGCCGAACATCGGGTGTAACCCCAGCACCGGGCCATCGTgc < 1:2613945/69‑1 (MQ=255) aCTTGCTTTGCCAGGCTACCGCTGTCCGGGCCGAACATCGGGTGTAACCCCAGCACCGGGCCATCGTgc < 1:2272174/69‑1 (MQ=255) aCTTGCTTTGCCAGGCTACCGCTGTCCGGGCCGAACATCGGGTGTAACCCCAGCACCGGGCCATCGTgc < 1:2489912/69‑1 (MQ=255) aCTTGCTTTGCCAGGCTACCGCTGTCCGGGCCGAACATCGGGTGTAACCCCAGCACCGGGCCATCGTgc < 1:2523424/69‑1 (MQ=255) aCTTGCTTTGCCAGGCTACCGCTGTCCGGGCCGAACATCGGGTGTAACCCCAGCACCGGGCCATCGTgc < 1:2586997/69‑1 (MQ=255) aCTTGCTTTGCCAGGCTACCGCTGTCCGGGCCGAACATCGGGTGTAACCCCAGCACCGGGCCATCGTgc < 1:258946/69‑1 (MQ=255) cTTGCTTTGCCAGGCTACCGCTGTCCGGACCGAACATCGGGTGTAGCCCCAGCACCGGACCATCATg > 1:1550591/1‑67 (MQ=255) ttGCTTTGCCAGGCTACCGCTGTCCGGACCGAACATCGGGTGTAGCCCCAGCACCGGACCATCATgcgc < 1:3134176/69‑1 (MQ=255) ttGCTTTGCCAGGCTACCGCTGTCCGGACCGAACATCGGGTGTAGCCCCAGCACCGGACCATCATgcgc < 1:1237605/69‑1 (MQ=255) ttGCTTTGCCAGGCTACCGCTGTCCGGACCGAACATCGGGTGTAGCCCCAGCACCGGACCATCATgcgc < 1:1458122/69‑1 (MQ=255) ttGCTTTGCCAGGCTACCGCTGTCCGGACCGAACATCGGGTGTAGCCCCAGCACCGGACCATCATgcgc < 1:2186042/69‑1 (MQ=255) ttGCTTTGCCAGGCTACCGCTGTCCGGACCGAACATCGGGTGTAGCCCCAGCACCGGACCATCATgcgc < 1:340171/69‑1 (MQ=255) ttGCTTTGCCAGGCTACCGCTGTCCGGACCGAACATCGGGTGTAGCCCCAGCACCGGACCATCATgcgc < 1:339484/69‑1 (MQ=255) ttGCTTTGCCAGGCTACCGCTGTCCGGACCGAACATCGGGTGTAGCCCCAGCACCGGACCATCATgcgc < 1:467946/69‑1 (MQ=255) gCTTTGCCAGTCTACCGCTGTCCGGACCGAACATCGGGTGTAGCCCCAGCACCGGACca < 1:407763/59‑1 (MQ=255) aCCGCTGTCCGGGCCGAACATCGGGTGTAACCCCAGCACCGGGCCATCGTgc < 1:698291/52‑1 (MQ=255) ccGCTGTCCGGGCCGAACATCGGGTGTAACCCCAGCACCGGGCCATCGTgc < 1:3000300/51‑1 (MQ=255) gCTTTCCGGACCGAACATCGGGTGTAGCCCCAGCACCGGACCATCATGCGCCACCATCATGGCCTGt < 1:2797062/67‑1 (MQ=255) gCTGTCCTGACCGAACATCGGGTGTAGCCCCAGCACCGGACCATCATGCCCCACCAGCATGGCCTGTa < 1:192885/68‑1 (MQ=255) gCTGTCCGGACCGAACATCGGGTGTAGCCCCAGCACCGGACCATCATGCGCCACCAGCATGGCCTGt < 1:1584121/67‑1 (MQ=255) gCTGTCCGGACCGAACATCGGGTGTAGCCCCAGCACCGGACCATCATGCGCCACCAGCATGGCCTGt < 1:1516406/67‑1 (MQ=255) gCTGTCCGGACCGAACATCGGGTGTAGCCCCAGCACCGGACCATCATGCGCCACCAGCATGGCCTGt < 1:3010793/67‑1 (MQ=255) gCTGTCCGGACCGAACATCGGGTGTAGCCCCAGCACCGGACCATCATGCGCCACCAGCATGGCCTGt < 1:893702/67‑1 (MQ=255) gCTGTCCGGACCGAACATCGGGTGTAGCCCCAGCACCGGACCATCATGCGCCACCAGCATGGCCTGt < 1:3106528/67‑1 (MQ=255) gCTGTCCGGACCGAACATCGGGTGTAGCCCCAGCACCGGACCATCATGCGCCACCAGCATGGCCTGt < 1:1248279/67‑1 (MQ=255) gCTGTCCGGACCGAACATCGGGTGTAGCCCCAGCACCGGACCATCATGCGCCACCAGCATGGCCTGt < 1:3170568/67‑1 (MQ=255) gCTGTCCGGACCGAACATCGGGTGTAGCCCCAGCACCGGACCATCATGCGCCACCAGCATGGCCTGt < 1:1037294/67‑1 (MQ=255) tCCGGACCGAACATCGGGTGTAGCCCCAGCACCGGACca < 1:577076/39‑1 (MQ=255) | ATGCTTCCGGTTTACGTCCATCACACCAGACCACAACTTGCTTTGCCAGGCTACCGCTGTCCGGACCGAACATCGGGTGTAGCCCCAGCACCGGACCATCATGCGCCACCAGCATGGCCTGTA > minE/1772089‑1772211 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |