| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 133,652 | A→G | 15.3% | A230A (GCT→GCC) | panB ← | 3‑methyl‑2‑oxobutanoate hydroxymethyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 133,652 | 0 | A | G | 15.3% | 184.9 / 18.9 | 110 | A230A (GCT→GCC) | panB | 3‑methyl‑2‑oxobutanoate hydroxymethyltransferase |
| Reads supporting (aligned to +/- strand): ref base A (54/39); new base G (11/6); total (65/45) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.90e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 110 total aligned reads displayed.
CACTTCAGCCATATACTGCCGCACAGCCGCGCGGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAGGAATGTGACCGCCGGTAATACCAAAGGCGTCGTGC > minE/133589‑133697 | cACTTCAGCCATATACTGCCGCACAGCCGCGCGGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGaa > 1:2272131/1‑69 (MQ=255)cACTTCAGCCATATACTGCCGCACAGCCGCGCGGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGaa > 1:2264353/1‑69 (MQ=255)cACTTCAGCCATATACTGCCGCACAGCCGCGCGGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGaa > 1:3056401/1‑69 (MQ=255)cACTTCAGCCATATACTGCCGCACAGCCGCGCGGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGaa > 1:379483/1‑69 (MQ=255)cACTTCAGCCATATACTGCCGCACAGCCGCGCGGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGa > 1:515378/1‑68 (MQ=255) tCAGCCATATACTGCCGCACAGCCGCGCGGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGAAttt > 1:2808687/1‑68 (MQ=255) ccATATACTGCCGCACAGCCGCGCGGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAGGaa < 1:719123/69‑1 (MQ=255) ccATATACTGCCGCACAGCCGCGCGGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAGGaa < 1:681094/69‑1 (MQ=255) ccATATACTGCCGCACAGCCGCGCGGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAGGaa < 1:1130491/69‑1 (MQ=255) ccATATACTGCCGCACAGCCGCGCGGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAGGaa < 1:1325689/69‑1 (MQ=255) cATATACTGCCTCACAGCCGCGCGGATGTCGCCCGTTTCCGCGAGGAAATTTTTAGCGAATTTAgg < 1:1666757/66‑1 (MQ=255) cATATACTGCCGCACAGCCGCGCGGATGTCGCCCGTTTCGGCGAGGAAATTTTTAg > 1:192143/1‑56 (MQ=255) cATATACTGCCGCACAGCCGCGCGGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAgg > 1:1494876/1‑66 (MQ=255) cATATACTGCCGCACAGCCGCGCGGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAgg < 1:2379160/66‑1 (MQ=255) cATATACTGCCGCACAGCCGCGCGGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAgg < 1:1605512/66‑1 (MQ=255) cATATACTGCCGCACAGCCGCGCGGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAgg < 1:1790971/66‑1 (MQ=255) cATATACTGCCGCACAGCCGCGCGGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAgg < 1:1877481/66‑1 (MQ=255) cATATACTGCCGCACAGCCGCGCGGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAgg < 1:2785556/66‑1 (MQ=255) cATATACTGCCGCACAGCCGCGCGGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAgg > 1:1023548/1‑66 (MQ=255) cATATACTGCCGCACAGCCGCGCGGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAgg < 1:1045226/66‑1 (MQ=255) cATATACTGCCGCACAGCCGCGCGGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAgg < 1:143802/66‑1 (MQ=255) ataCTGCCGCACAGCCGCGCGGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAGGAAtgtg < 1:2387989/69‑1 (MQ=255) ataCTGCCGCACAGCCGCGCGGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAGGAAtgtg < 1:172023/69‑1 (MQ=255) ataCTGCCGCACAGCCGCGCGGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAGGAAtgtg < 1:1072764/69‑1 (MQ=255) taCTGCCGCACAGCCGCGCGGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGGGAATTTAGGAATGTGa > 1:2310827/1‑69 (MQ=255) taCTGCCGCACAGCCGCGCGGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGaa > 1:3098932/1‑56 (MQ=255) taCTGCCGCACAGCCGCGCGGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGaa > 1:356528/1‑56 (MQ=255) taCTGCCGCACAGCCGCGCGGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGaa > 1:153453/1‑56 (MQ=255) taCTGCCGCACAGCCGCGCGGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGaa > 1:361459/1‑56 (MQ=255) taCTGCCGCACAGCCGCGCGGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGaa > 1:1738107/1‑56 (MQ=255) taCTGCCGCACAGCCGCGCGGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGaa > 1:772266/1‑56 (MQ=255) taCTGCCGCACAGCCGCGCGGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAGGAAtgtg > 1:3424369/1‑68 (MQ=255) taCTGCCGCACAGCCGCGCGGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAGGAAtgt < 1:2799401/67‑1 (MQ=255) taCTGCCGCACAGCCGCGCGGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAGGAAtgt < 1:2572229/67‑1 (MQ=255) taCTGCCGCACAGCCGCGCGGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAGGAATGTGa > 1:2478893/1‑69 (MQ=255) taCTGCCGCACAGCCGCGCGGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAGGAATGTGa > 1:1016487/1‑69 (MQ=255) taCTGCCGCACAGCCGCGCGGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAGGAATGTGa > 1:421463/1‑69 (MQ=255) taCTGCCGCACAGCCGCGCGGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAGGAATGTGa > 1:587515/1‑69 (MQ=255) taCTGCCGCACAGCCGCGCGGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAGGAATGTGa > 1:2402522/1‑69 (MQ=255) taCTGCCGCACAGCCGCGCGGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAGGAATGTGa > 1:2420758/1‑69 (MQ=255) taCTGCCGCACAGCCGCGCGGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAGGAATGTGa > 1:77487/1‑69 (MQ=255) taCTGCCGCACAGCCGCGCGGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAGGAATGTGa > 1:3019070/1‑69 (MQ=255) taCTGCCGCACAGCCGCGCGGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAGGAATGTGa > 1:2454259/1‑69 (MQ=255) taCTGCCGCACAGCCGCGCGGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAGGAATGTGa > 1:685255/1‑69 (MQ=255) taCTGCCGCACAGCCGCGCGGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAGGAATGTGa > 1:2569973/1‑69 (MQ=255) taCTGCCGCACAGCCGCGCGGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAGGAATGTGa > 1:294779/1‑69 (MQ=255) taCTGCCGCACAGCCGCGCGGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAGGAATGTGa > 1:265410/1‑69 (MQ=255) taCTGCCGCACAGCCGCGCGGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAGGAATGTGa > 1:2698521/1‑69 (MQ=255) taCTGCCGCACAGCCGCGCGGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAGGAATGTGa > 1:2710593/1‑69 (MQ=255) taCTGCCGCACAGCCGCGCGGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAGGAATGTGa > 1:670158/1‑69 (MQ=255) taCTGCCGCACAGCCGCGCGGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAGGAATGTGa > 1:2885652/1‑69 (MQ=255) taCTGCCGCACAGCCGCGCGGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAGGAATGTGa > 1:228127/1‑69 (MQ=255) taCTGCCGCACAGCCGCGCGGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAGGAATGTGa > 1:2279654/1‑69 (MQ=255) taCTGCCGCACAGCCGCGCGGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAGGAATGTGa > 1:1046338/1‑69 (MQ=255) taCTGCCGCACAGCCGCGCGGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAGGAATGTGa > 1:2087705/1‑69 (MQ=255) taCTGCCGCACAGCCGCGCGGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAGGAATGTGa > 1:1138996/1‑69 (MQ=255) taCTGCCGCACAGCCGCGCGGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAGGAATGTGa > 1:1372713/1‑69 (MQ=255) taCTGCCGCACAGCCGCGCGGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAGGAATGTGa > 1:2052771/1‑69 (MQ=255) taCTGCCGCACAGCCGCGCGGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAGGAATGTGa > 1:1405073/1‑69 (MQ=255) taCTGCCGCACAGCCGCGCGGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAGGAATGTGa > 1:2010848/1‑69 (MQ=255) taCTGCCGCACAGCCGCGCGGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAGGAATGTGa > 1:1415267/1‑69 (MQ=255) taCTGCCGCACAGCCGCGCGGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAGGAATGTGa > 1:1936770/1‑69 (MQ=255) taCTGCCGCACAGCCGCGCGGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAGGAATGTGa > 1:1438063/1‑69 (MQ=255) taCTGCCGCACAGCCGCGCGGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAGGAATGTGa > 1:1486506/1‑69 (MQ=255) taCTGCCGCACAGCCGCGCGGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAGGAATGTGa > 1:1755591/1‑69 (MQ=255) taCTGCCGCACAGCCACGCGGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAGGAATGTGa > 1:377213/1‑69 (MQ=255) cacaGCCGCGCGTATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAGGAATGTGACCGCCGGt < 1:831421/69‑1 (MQ=255) cacaGCCGCGCGGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAGGAATGTGACCGCCGGt < 1:2625244/69‑1 (MQ=255) cacaGCCGCGCGGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAGGAATGTGACCGCCGGt < 1:1836560/69‑1 (MQ=255) cacaGCCGCGCGGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAGGAATGTGACCGCCGGt < 1:2838098/69‑1 (MQ=255) cacaGCCGCGCGGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAGGAATGTGACCGCCGGt < 1:159999/69‑1 (MQ=255) cacaGCCGCGCGGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAGGAATGTGACCGCCGGt < 1:1083309/69‑1 (MQ=255) cacaGCCGCGCGGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAGGAATGTGACCGCCGGt < 1:1025143/69‑1 (MQ=255) acaGCCGCGCGGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAGGAATGTGACCGCCGGTa < 1:2558379/69‑1 (MQ=255) gcgGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAGGAATGTGACCGCCGGTAATACCaaa < 1:626881/69‑1 (MQ=255) gcgGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAGGAATGTGACCGCCGGTAATACCaaa < 1:191674/69‑1 (MQ=255) cgGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAGGAATGTGACCGCCGGTAATACCaaa < 1:2073569/68‑1 (MQ=255) gATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAGGAATGTGACCGCCGGTAATACCaaa < 1:1396016/66‑1 (MQ=255) aTGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAGGAAtgtg < 1:2880742/47‑1 (MQ=255) tCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAGGAATGTGACCGCCGGt < 1:327985/53‑1 (MQ=255) tCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAGGAATGTGACCGCCGGt < 1:1680878/53‑1 (MQ=255) tCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAGGAATGTGACCGCCGGt < 1:2032742/53‑1 (MQ=255) tCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAGGAATGTGACCGCCGGt < 1:600506/53‑1 (MQ=255) tCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAGGAATGTGACCGCCGGt < 1:381288/53‑1 (MQ=255) ccGTTTCGGCGAGGAAATTTTTGGCGATTTTAGGAATATGACCGCCGGTAATGCCGAACGCGTCGTGc > 1:3106266/1‑68 (MQ=255) ccGTTTCGGCGAGGAAATTTTTGGCGAATTTAGGAATATGACCGCCGGTAAt > 1:2822524/1‑52 (MQ=255) ccGTTTCGGCGAGGAAATTTTTGGCGAATTTAGGAATATGACCGCCGGTAATGCCGAACGCGTCGTGc > 1:2251736/1‑68 (MQ=255) ccGTTTCGGCGAGGAAATTTTTGGCGAATTTAGGAATATGACCGCCGGTAATGCCGAACGCGTCGTGc > 1:1005951/1‑68 (MQ=255) ccGTTTCGGCGAGGAAATTTTTGGCGAATTTAGGAATATGACCGCCGGTAATGCCGAACGCGTCGTGc > 1:2313557/1‑68 (MQ=255) ccGTTTCGGCGAGGAAATTTTTGGCGAATTTAGGAATATGACCGCCGGTAATGCCGAACGCGTCGTGc > 1:1179660/1‑68 (MQ=255) ccGTTTCGGCGAGGAAATTTTTGGCGAATTTAGGAATATGACCGCCGGTAATGCCGAACGCGTCGTGc > 1:37291/1‑68 (MQ=255) ccGTTTCGGCGAGGAAATTTTTGGCGAATTTAGGAATATGACCGCCGGTAATGCCGAACGCGTCGTGc > 1:3235588/1‑68 (MQ=255) ccGTTTCGGCGAGGAAATTTTTGGCGAATTTAGGAATATGACCGCCGGTAATGCCGAACGCGTCGTGc > 1:3185346/1‑68 (MQ=255) ccGTTTCGGCGAGGAAATTTTTGGCGAATTTAGGAATATGACCGCCGGTAATGCCGAACGCGTCGTGc > 1:1905814/1‑68 (MQ=255) gCGAGGAAATTTTTAGCGAATTTAGGAATGTGACCGCCGGt < 1:3249705/41‑1 (MQ=255) aaTTTTTGGCGAATTTAGGAATATGACCGCCGGTAAtg < 1:368518/38‑2 (MQ=38) aaTTTTTGGCGAATTTAGGAATATGACCGCCGGTAAtg < 1:1943256/38‑2 (MQ=38) aaTTTTTGGCGAATTTAGGAATATGACCGCCGGTAAtg < 1:110853/38‑2 (MQ=38) aaTTTTTGGCGAATTTAGGAATATGACCGCCGGTAAtg < 1:1061491/38‑2 (MQ=38) aaTTTTTGGCGAATTTAGGAATATGACCGCCGGTAAtg < 1:2970030/38‑2 (MQ=38) | CACTTCAGCCATATACTGCCGCACAGCCGCGCGGATGTCGCCCGTTTCGGCGAGGAAATTTTTAGCGAATTTAGGAATGTGACCGCCGGTAATACCAAAGGCGTCGTGC > minE/133589‑133697 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |