| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,772,441 | G→A | 47.4% | G105G (GGC→GGT) | tyrA ← | fused chorismate mutase T and prephenate dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,772,441 | 0 | G | A | 47.4% | 3.9 / 50.1 | 62 | G105G (GGC→GGT) | tyrA | fused chorismate mutase T and prephenate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base G (29/3); new base A (17/12); total (46/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.36e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GAATCCGCACCTGATAACCCGAGAGGGTCAGCATCTTCTCGAACAGGCGTCCCATCTGACCGCCACCGCCGACGATAACCACCGGACGCAGTGACGGACAAAGTGTTTTAAATCCTTTGTCGTTTTCACTGGA > minE/1772374‑1772506 | gAATCCGCACCTGATAACCCGAGAGGGTCAGCATCTTCTCGAACAGGCGTCCCATCTGaccgccaccg > 1:2360466/1‑68 (MQ=255)gAATCCGCACCTGATAACCCGAGAGGGTCAGCATCTTCTCGAACAGGCGTCCCATCTGaccgccaccg > 1:931308/1‑68 (MQ=255)gAATCCGCACCTGATAACCCGAGAGGGTCAGCATCTTCTCGAACAGGCGTCCCATCTGaccgccaccg > 1:462626/1‑68 (MQ=255)gAATCCGCACCTGATAACCCGAGAGGGTCAGCATCTTCTCGAACAGGCGTCCCATCTGaccgccaccg > 1:1286851/1‑68 (MQ=255)gAATCCGCACCTGATAACCCGAGAGGGTCAGCATCTTCTCGAACAGGCGTCCCATCTGaccgccaccg > 1:111596/1‑68 (MQ=255) cACCTGATAACCCGAGAGGGTCAGCATCTTCTCGAACAGGCGTCCCATCTGACCGCCACCGCCGACGAt < 1:295677/69‑1 (MQ=255) cACCTGATAACCCGAGAGGGTCAGCATCTTCTCGAACAGGCGTCCCATCTGACCGCCACCGCCGACGAt < 1:2117046/69‑1 (MQ=255) cACCTGATAACCCGAGAGGGTCAGCATCTTCTCGAACAGGCGTCCCATCTGACCGCCACCGCCGACGAt < 1:2663115/69‑1 (MQ=255) aaCCCGAGAGGGTCAGCATCTTCTCGAACAGGCGTCCCATCTGACCGCCACCGCCGACGATAACCACCg > 1:2062973/1‑69 (MQ=255) aaCCCGAGAGGGTCAGCATCTTCTCGAACAGGCGTCCCATCTGACCGCCACCGCCGACGATAACCACCg > 1:221164/1‑69 (MQ=255) aaCCCGAGAGGGTCAGCATCTTCTCGAACAGGCGTCCCATCTGACCGCCACCGCCGACGATAACCACCg > 1:2654365/1‑69 (MQ=255) aaCCCGAGAGGGTCAGCATCTTCTCGAACAGGCGTCCCATCTGACCGCCACCGCCGACGATAACCACCg > 1:663132/1‑69 (MQ=255) aaCCCGAGAGGGTCAGCATCTTCTCGAACAGGCGTCCCATCTGACCGCCACCGCCGACGATAACCACCg > 1:2827060/1‑69 (MQ=255) ttctCGAACAGGCGTCCCATCTGACCGCCACCGCCGACGATAacc > 1:1119693/1‑45 (MQ=255) ttctCGAACAGGCGTCCCATCTGACCGCCACCGCCGACGATAacc > 1:2869859/1‑45 (MQ=255) ttctCGAACAGGCGTCCCATCTGACCGCCACCGCCGACGATAacc > 1:918375/1‑45 (MQ=255) ttctCGAACAGGCGTCCCATCTGACCGCCACCGCCGACGATAacc > 1:864050/1‑45 (MQ=255) ttctCGAACAGGCGTCCCATCTGACCGCCACCGCCGACGATAacc > 1:1243702/1‑45 (MQ=255) ttctCGAACAGGCGTCCCATCTGACCGCCACCGCCGACGATAacc > 1:2948559/1‑45 (MQ=255) ttctCGAACAGGCGTCCCATCTGACCGCCACCGCCGACGATAACCACCGGACGCAGTGACGGACAAAgt > 1:1483091/1‑69 (MQ=255) ttctCGAACAGGCGTCCCATCTGACCGCCACCGCCGACGATAACCACCGGACGCAGTGACGGACAAAgt > 1:447387/1‑69 (MQ=255) ttctCGAACAGGCGTCCCATCTGACCGCCACCGCCGACGATAACCACCGGACGCAGTGACGGACAAAgt > 1:1519772/1‑69 (MQ=255) ttctCGAACAGGCGTCCCATCTGACCGCCACCGCCGACGATAACCACCGGACGCAGTGACGGACAAAgt > 1:1638920/1‑69 (MQ=255) ttctCGAACAGGCGTCCCATCTGACCGCCACCGCCGACGATAACCACCGGACGCAGTGACGGACAAAgt > 1:3250301/1‑69 (MQ=255) ttctCGAACAGGCGTCCCATCTGACCGCCACCGCCGACGATAACCACCGGACGCAGTGACGGACAAAgt > 1:2288347/1‑69 (MQ=255) ttctCGAACAGGCGTCCCATCTGACCGCCACCGCCGACGATAACCACCGGACGCAGTGACGGACAAAgt > 1:1825571/1‑69 (MQ=255) aCAGACGTCCCATCTGACCGCCACCACCGACGATAACCACCGGGCGCAGTGACGGACAAAGCGttt < 1:2985776/66‑1 (MQ=255) aCAGACGTCCCATCTGACCGCCACCACCGACGATAACCACCGGGCGCAGTGACGGACAAAGCGttt < 1:2230251/66‑1 (MQ=255) aCAGACGTCCCATCTGACCGCCACCACCGACGATAACCACCGGGCGCAGTGACGGACAAAGCGttt < 1:320310/66‑1 (MQ=255) aCAGACGTCCCATCTGACCGCCACCACCGACGATAACCACCGGGCGCAGTGACGGACAAAGCGttt < 1:3101652/66‑1 (MQ=255) aCAGACGTCCCATCTGACCGCCACCACCGACGATAACCACCGGGCGCAGTGACGGACAAAGCGttt < 1:3048140/66‑1 (MQ=255) aCAGACGTCCCATCTGACCGCCACCACCGACGATAACCACCGGGCGCAGTGACGGACAAAGCGttt < 1:2934321/66‑1 (MQ=255) aCAGACGTCCCATCTGACCGCCACCACCGACGATAACCACCGGGCGCAGTGACGGACAAAGCGttt < 1:604311/66‑1 (MQ=255) aCAGACGTCCCATCTGACCGCCACCACCGACGATAACCACCGGGCGCAGTGACGGACAAAGCGttt < 1:3283072/66‑1 (MQ=255) aCAGACGTCCCATCTGACCGCCACCACCGACGATAACCACCGGGCGCAGTGACGGACAAAGCGttt < 1:1437001/66‑1 (MQ=255) aCAGACGTCCCATCTGACCGCCACCACCGACGATAACCACCGGGCGCAGTGACGGACAAAGCGttt < 1:3325458/66‑1 (MQ=255) aCAGACGTCCCATCTGACCGCCACCACCGACGATAACCACCGGGCGCAGTGACGGACAAAGCGttt < 1:1691392/66‑1 (MQ=255) cAGGCGTCCCATCTGACCGCCACCGCCGACGATAACCACCGGACGCAGTGACGGACAAAGTGTTTTaaa > 1:2500988/1‑69 (MQ=255) cAGGCGTCCCATCTGACCGCCACCGCCGACGATAACCACCGGACGCAGTGACGGACAAAGTGTTTTaaa > 1:689157/1‑69 (MQ=255) cAGGCGTCCCATCTGACCGCCACCGCCGACGATAACCACCGGACGCAGTGACGGACAAAGTGTTTTaaa > 1:3207910/1‑69 (MQ=255) cAGGCGTCCCATCTGACCGCCACCGCCGACGATAACCACCGGACGCAGTGACGGACAAAGTGTTTTaaa > 1:2251576/1‑69 (MQ=255) cAGGCGTCCCATCTGACCGCCACCGCCGACGATAACCACCGGACGCAGTGACGGACAAAGTGTTTTaaa > 1:1968670/1‑69 (MQ=255) cAGACGTCCCATCTGACCGCCACCTCCGACGATAACCACCGGGCGCAGTGACGGACAAAGCGttt < 1:467429/65‑1 (MQ=255) cAGACGTCCCATCTGACCGCCACCACCGACGATAACCACCGGGCGCAGTGACGGACAAAGCGttt < 1:160625/65‑1 (MQ=255) gTCCCATCTGACCGCCCCCACCGACGATAACCACCGGGCGCAGTGCCGGACAAAg > 1:293356/1‑55 (MQ=255) gTCCCATCTGACCGCCACCGCCGACGATATCCCCCGGACGCAGTGACGGACAAAGTGTTTTAAATCCt > 1:3322194/1‑68 (MQ=255) gTCCCATCTGACCGCCACCGCCGACGATAACCACCGGACGCAGTg > 1:2979957/1‑45 (MQ=255) gTCCCATCTGACCGCCACCGCCGACGATAACCACCGGACGCAGTGACGGACAAAGTGTTTTAAATCCtt > 1:1426030/1‑69 (MQ=255) gTCCCATCTGACCGCCACCGCCGACGATAACCACCGGACGCAGTGACGGACAAAGTGTTTTAAATCCtt > 1:2043645/1‑69 (MQ=255) gTCCCATCTGACCGCCACCGCCGACGATAACCACCGGACGCAGTCACCGACAAAg > 1:2729880/1‑55 (MQ=255) gTCCCATCTGACCGCCACCACCGACGATAACCACCGGGCGCAGTGACGGACAAAGCGTTTTAAATCCtt > 1:1066031/1‑69 (MQ=255) gTCCCATCTGACCGCCACCACCGACGATAACCACCGGGCGCAGTGACGGACAAAGCGTTTTAAATCCtt > 1:1139743/1‑69 (MQ=255) gTCCCATCTGACCGCCACCACCGACGATAACCACCGGGCGCAGTGACGGACAAAGCGTTTTAAATCCtt > 1:472808/1‑69 (MQ=255) gTCCCATCTGACCGCCACCACCGACGATAACCACCGGGCGCAGTGACGGACAAAGCGTTTTAAATCCtt > 1:3139814/1‑69 (MQ=255) gTCCCATCTGACCGCCACCACCGACGATAACCACCGGGCGCAGTGACGGACAAAGCGTTTTAAATCCtt > 1:3317455/1‑69 (MQ=255) gTCCCATCTGACCGCCACCACCGACGATAACCACCGGGCGCAGTGACGGACAAAGCGTTTTAAATCCtt > 1:3252035/1‑69 (MQ=255) gTCCCATCTGACCGCCACCACCGACGATAACCACCGGGCGCAGTGACGGACAAAGCGTTTTAAATCCtt > 1:2729556/1‑69 (MQ=255) gTCCCATCTGACCGCCACCACCGACGATAACCACCGGGCGCAGTGACGGACAAAGCGTTTTAAATCCtt > 1:1187600/1‑69 (MQ=255) gTCCCATCTGACCGCCACCACCGACGATAACCACCGGGCGCAGTGACGGACAAAGCGTTTTAAATCCtt > 1:1355197/1‑69 (MQ=255) gTCCCATCTGACCGCCACCACCGACGATAACCACCGGGCGCAGTGACGGACAAAGCGTTTTAAATCCtt > 1:1450815/1‑69 (MQ=255) gTCCCATCTGACCGCCACCACCGACGATAACCACCGGGCGCAGTGACGGACAAAGCGTTTTAAATCCtt > 1:1453420/1‑69 (MQ=255) gTCCCATCTGACCGCCACCACCGACGATAACCACCGGGCGCAGTGACGGACAAAGCGTTTTAAATCCtt > 1:1764543/1‑69 (MQ=255) gTCCCATCTGACCGCCACCACCGACGATAACCACCGGGCGCAGTGACGGACAAAGCGTTTTAAATCCtt > 1:1700192/1‑69 (MQ=255) gTCCCATCTGACCGCCACCACCGACGATAACCACCGGGCGCAGTGACGGACAAAGCGTTTTAAATCCtt > 1:1512809/1‑69 (MQ=255) gTCCCATCTGACCGCCACCACCGACGATAACCACCGGGCGCAGTGACGGACAAAGCGTTTTAAATCCtt > 1:2759034/1‑69 (MQ=255) gTCCCATCTGACCGCCACCACCGACGATAACCAACGGGCGCAGTGACGGACAAAGCGTTTTAAATCCtt > 1:249395/1‑69 (MQ=255) tGACCGCCACCGCCGACGATAACCACCGGACGCAGTGACGGACAAAGTGTTTTAAATCCTTTGTCGtt > 1:1115328/1‑68 (MQ=255) ccaccgccGACGATAACCACCGGACGCAGTGACGGACAAAGTGTTTTAAATCCTTTGTCGTTTTCACTg < 1:1492463/69‑1 (MQ=255) ccaccgccGACGATAACCACCGGACGCAGTGACGGACAAAGTGTTTTAAATCCTTTGTCGTTTTCACTg < 1:1212789/69‑1 (MQ=255) ccaccgccGACGATAACCACCGGACGCAGTGACGGACAAAGTGTTTTAAATCCTTTGTCGTTTTCACTg < 1:46402/69‑1 (MQ=255) ccaccgccGACGATAACCACCGGACGCAGTGACGGACAAAGTGTTTTAAATCCTTTGTCGTTTTCACTg < 1:199141/69‑1 (MQ=255) ccaccgccGACGATAACCACCGGACGCAGTGACGGACAAAGTGTTTTAAATCCTTTGTCGTTTTCACTg < 1:2117597/69‑1 (MQ=255) ccaccgccGACGATAACCACCGGACGCAGTGACGGACAAAGTGTTTTAAATCCTTTGTCGTTTTCACTg < 1:2328144/69‑1 (MQ=255) ccaccgccGACGATAACCACCGGACGCAGTGACGGACAAAGTGTTTTAAATCCTTTGTCGTTTTCACTg < 1:1024337/69‑1 (MQ=255) gccgACGATAACCACCGGACGCAGTGACGGACAAAGTGTTTTAAATCCTTTGTCGTTTTCACTgg > 1:1255177/1‑65 (MQ=255) gccgACGATAACCACCGGACGCAGTGACGGACAAAGTGTTTTAAATCCTTTGTCGTTTTCACTGGa > 1:1163903/1‑66 (MQ=255) gccgACGATAACCACCGGACGCAGTGACGGACAAAGTGTTTTAAATCCTTTGTCGTTTTCACTGGa > 1:1161377/1‑66 (MQ=255) gccgACGATAACCACCGGACGCAGTGACGCACAAAGTGTTTTAAATCCTTTGTCGTTTTCACTGGa > 1:2438299/1‑66 (MQ=255) | GAATCCGCACCTGATAACCCGAGAGGGTCAGCATCTTCTCGAACAGGCGTCCCATCTGACCGCCACCGCCGACGATAACCACCGGACGCAGTGACGGACAAAGTGTTTTAAATCCTTTGTCGTTTTCACTGGA > minE/1772374‑1772506 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |