| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,772,477 | T→C | 43.3% | T93T (ACA→ACG) | tyrA ← | fused chorismate mutase T and prephenate dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,772,477 | 0 | T | C | 43.3% | 14.6 / 51.1 | 67 | T93T (ACA→ACG) | tyrA | fused chorismate mutase T and prephenate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base T (20/18); new base C (16/13); total (36/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTCTCGAACAGGCGTCCCATCTGACCGCCACCGCCGACGATAACCACCGGACGCAGTGACGGACAAAGTGTTTTAAATCCTTTGTCGTTTTCACTGGAGTAAGATTCACGC > minE/1772409‑1772519 | ttctCGAACAGGCGTCCCATCTGACCGCCACCGCCGACGATAACCACCGGACGCAGTGACGGACAAAgt > 1:1825571/1‑69 (MQ=255)ttctCGAACAGGCGTCCCATCTGACCGCCACCGCCGACGATAACCACCGGACGCAGTGACGGACAAAgt > 1:447387/1‑69 (MQ=255)ttctCGAACAGGCGTCCCATCTGACCGCCACCGCCGACGATAACCACCGGACGCAGTGACGGACAAAgt > 1:1638920/1‑69 (MQ=255)ttctCGAACAGGCGTCCCATCTGACCGCCACCGCCGACGATAACCACCGGACGCAGTGACGGACAAAgt > 1:1519772/1‑69 (MQ=255)ttctCGAACAGGCGTCCCATCTGACCGCCACCGCCGACGATAACCACCGGACGCAGTGACGGACAAAgt > 1:2288347/1‑69 (MQ=255)ttctCGAACAGGCGTCCCATCTGACCGCCACCGCCGACGATAACCACCGGACGCAGTGACGGACAAAgt > 1:1483091/1‑69 (MQ=255)ttctCGAACAGGCGTCCCATCTGACCGCCACCGCCGACGATAACCACCGGACGCAGTGACGGACAAAgt > 1:3250301/1‑69 (MQ=255) aCAGACGTCCCATCTGACCGCCACCACCGACGATAACCACCGGGCGCAGTGACGGACAAAGCGttt < 1:3101652/66‑1 (MQ=255) aCAGACGTCCCATCTGACCGCCACCACCGACGATAACCACCGGGCGCAGTGACGGACAAAGCGttt < 1:3325458/66‑1 (MQ=255) aCAGACGTCCCATCTGACCGCCACCACCGACGATAACCACCGGGCGCAGTGACGGACAAAGCGttt < 1:2230251/66‑1 (MQ=255) aCAGACGTCCCATCTGACCGCCACCACCGACGATAACCACCGGGCGCAGTGACGGACAAAGCGttt < 1:1691392/66‑1 (MQ=255) aCAGACGTCCCATCTGACCGCCACCACCGACGATAACCACCGGGCGCAGTGACGGACAAAGCGttt < 1:3283072/66‑1 (MQ=255) aCAGACGTCCCATCTGACCGCCACCACCGACGATAACCACCGGGCGCAGTGACGGACAAAGCGttt < 1:1437001/66‑1 (MQ=255) aCAGACGTCCCATCTGACCGCCACCACCGACGATAACCACCGGGCGCAGTGACGGACAAAGCGttt < 1:604311/66‑1 (MQ=255) aCAGACGTCCCATCTGACCGCCACCACCGACGATAACCACCGGGCGCAGTGACGGACAAAGCGttt < 1:3048140/66‑1 (MQ=255) aCAGACGTCCCATCTGACCGCCACCACCGACGATAACCACCGGGCGCAGTGACGGACAAAGCGttt < 1:2985776/66‑1 (MQ=255) aCAGACGTCCCATCTGACCGCCACCACCGACGATAACCACCGGGCGCAGTGACGGACAAAGCGttt < 1:2934321/66‑1 (MQ=255) aCAGACGTCCCATCTGACCGCCACCACCGACGATAACCACCGGGCGCAGTGACGGACAAAGCGttt < 1:320310/66‑1 (MQ=255) cAGGCGTCCCATCTGACCGCCACCGCCGACGATAACCACCGGACGCAGTGACGGACAAAGTGTTTTaaa > 1:1968670/1‑69 (MQ=255) cAGGCGTCCCATCTGACCGCCACCGCCGACGATAACCACCGGACGCAGTGACGGACAAAGTGTTTTaaa > 1:2500988/1‑69 (MQ=255) cAGGCGTCCCATCTGACCGCCACCGCCGACGATAACCACCGGACGCAGTGACGGACAAAGTGTTTTaaa > 1:3207910/1‑69 (MQ=255) cAGGCGTCCCATCTGACCGCCACCGCCGACGATAACCACCGGACGCAGTGACGGACAAAGTGTTTTaaa > 1:2251576/1‑69 (MQ=255) cAGGCGTCCCATCTGACCGCCACCGCCGACGATAACCACCGGACGCAGTGACGGACAAAGTGTTTTaaa > 1:689157/1‑69 (MQ=255) cAGACGTCCCATCTGACCGCCACCTCCGACGATAACCACCGGGCGCAGTGACGGACAAAGCGttt < 1:467429/65‑1 (MQ=255) cAGACGTCCCATCTGACCGCCACCACCGACGATAACCACCGGGCGCAGTGACGGACAAAGCGttt < 1:160625/65‑1 (MQ=255) gTCCCATCTGACCGCCACCGCCGACGATATCCCCCGGACGCAGTGACGGACAAAGTGTTTTAAATCCt > 1:3322194/1‑68 (MQ=255) gTCCCATCTGACCGCCACCGCCGACGATAACCACCGGACGCAGTGACGGACAAAGTGTTTTAAATCCtt > 1:2043645/1‑69 (MQ=255) gTCCCATCTGACCGCCACCGCCGACGATAACCACCGGACGCAGTGACGGACAAAGTGTTTTAAATCCtt > 1:1426030/1‑69 (MQ=255) gTCCCATCTGACCGCCACCACCGACGATAACCACCGGGCGCAGTGACGGACAAAGCGTTTTAAATCCtt > 1:3252035/1‑69 (MQ=255) gTCCCATCTGACCGCCACCACCGACGATAACCACCGGGCGCAGTGACGGACAAAGCGTTTTAAATCCtt > 1:2759034/1‑69 (MQ=255) gTCCCATCTGACCGCCACCACCGACGATAACCACCGGGCGCAGTGACGGACAAAGCGTTTTAAATCCtt > 1:3317455/1‑69 (MQ=255) gTCCCATCTGACCGCCACCACCGACGATAACCACCGGGCGCAGTGACGGACAAAGCGTTTTAAATCCtt > 1:2729556/1‑69 (MQ=255) gTCCCATCTGACCGCCACCACCGACGATAACCACCGGGCGCAGTGACGGACAAAGCGTTTTAAATCCtt > 1:3139814/1‑69 (MQ=255) gTCCCATCTGACCGCCACCACCGACGATAACCACCGGGCGCAGTGACGGACAAAGCGTTTTAAATCCtt > 1:1764543/1‑69 (MQ=255) gTCCCATCTGACCGCCACCACCGACGATAACCACCGGGCGCAGTGACGGACAAAGCGTTTTAAATCCtt > 1:1700192/1‑69 (MQ=255) gTCCCATCTGACCGCCACCACCGACGATAACCACCGGGCGCAGTGACGGACAAAGCGTTTTAAATCCtt > 1:1066031/1‑69 (MQ=255) gTCCCATCTGACCGCCACCACCGACGATAACCACCGGGCGCAGTGACGGACAAAGCGTTTTAAATCCtt > 1:1512809/1‑69 (MQ=255) gTCCCATCTGACCGCCACCACCGACGATAACCACCGGGCGCAGTGACGGACAAAGCGTTTTAAATCCtt > 1:1139743/1‑69 (MQ=255) gTCCCATCTGACCGCCACCACCGACGATAACCACCGGGCGCAGTGACGGACAAAGCGTTTTAAATCCtt > 1:1453420/1‑69 (MQ=255) gTCCCATCTGACCGCCACCACCGACGATAACCACCGGGCGCAGTGACGGACAAAGCGTTTTAAATCCtt > 1:1187600/1‑69 (MQ=255) gTCCCATCTGACCGCCACCACCGACGATAACCACCGGGCGCAGTGACGGACAAAGCGTTTTAAATCCtt > 1:1450815/1‑69 (MQ=255) gTCCCATCTGACCGCCACCACCGACGATAACCACCGGGCGCAGTGACGGACAAAGCGTTTTAAATCCtt > 1:472808/1‑69 (MQ=255) gTCCCATCTGACCGCCACCACCGACGATAACCACCGGGCGCAGTGACGGACAAAGCGTTTTAAATCCtt > 1:1355197/1‑69 (MQ=255) gTCCCATCTGACCGCCACCACCGACGATAACCAACGGGCGCAGTGACGGACAAAGCGTTTTAAATCCtt > 1:249395/1‑69 (MQ=255) tGACCGCCACCGCCGACGATAACCACCGGACGCAGTGACGGACAAAGTGTTTTAAATCCTTTGTCGtt > 1:1115328/1‑68 (MQ=255) ccaccgccGACGATAACCACCGGACGCAGTGACGGACAAAGTGTTTTAAATCCTTTGTCGTTTTCACTg < 1:1024337/69‑1 (MQ=255) ccaccgccGACGATAACCACCGGACGCAGTGACGGACAAAGTGTTTTAAATCCTTTGTCGTTTTCACTg < 1:199141/69‑1 (MQ=255) ccaccgccGACGATAACCACCGGACGCAGTGACGGACAAAGTGTTTTAAATCCTTTGTCGTTTTCACTg < 1:1212789/69‑1 (MQ=255) ccaccgccGACGATAACCACCGGACGCAGTGACGGACAAAGTGTTTTAAATCCTTTGTCGTTTTCACTg < 1:2328144/69‑1 (MQ=255) ccaccgccGACGATAACCACCGGACGCAGTGACGGACAAAGTGTTTTAAATCCTTTGTCGTTTTCACTg < 1:1492463/69‑1 (MQ=255) ccaccgccGACGATAACCACCGGACGCAGTGACGGACAAAGTGTTTTAAATCCTTTGTCGTTTTCACTg < 1:46402/69‑1 (MQ=255) ccaccgccGACGATAACCACCGGACGCAGTGACGGACAAAGTGTTTTAAATCCTTTGTCGTTTTCACTg < 1:2117597/69‑1 (MQ=255) gccgACGATAACCACCGGACGCAGTGACGGACAAAGTGTTTTAAATCCTTTGTCGTTTTCACTgg > 1:1255177/1‑65 (MQ=255) gccgACGATAACCACCGGACGCAGTGACGGACAAAGTGTTTTAAATCCTTTGTCGTTTTCACTGGa > 1:1163903/1‑66 (MQ=255) gccgACGATAACCACCGGACGCAGTGACGGACAAAGTGTTTTAAATCCTTTGTCGTTTTCACTGGa > 1:1161377/1‑66 (MQ=255) gccgACGATAACCACCGGACGCAGTGACGCACAAAGTGTTTTAAATCCTTTGTCGTTTTCACTGGa > 1:2438299/1‑66 (MQ=255) gaTAACCACCGGACGCAGTGATGGACAAAGTGTTTTAAATCCTTTGTCGTTTTCACTGGAGt < 1:1041551/62‑1 (MQ=255) gaTAACCACCGGACGCAGTGACGGACAAAGTGTTTTAAATCCTTTGTCTTTTTCACTGGAGt < 1:2324528/62‑1 (MQ=255) gaTAACCACCGGACGCAGTGACGGACAAAGTGTTTTAAATCCTTTGTCGTTTTCACTGGAGt < 1:1778403/62‑1 (MQ=255) gaTAACCACCGGACGCAGTGACGGACAAAGTGTTTTAAATCCTTTGTCGTTTTCACTGGAGt < 1:853273/62‑1 (MQ=255) gaTAACCACCGGACGCAGTGACGGACAAAGTGTTTTAAATCCTTTGTCGTTTTCACTGGAGt < 1:1159960/62‑1 (MQ=255) gaTAACCACCGGACGCAGTGACGGACAAAGTGTTTTAAATCCTTTGTCGTTTTCACTGGAGt < 1:1418679/62‑1 (MQ=255) gaTAACCACCGGACGCAGTGACGGACAAAGTGTTTTAAATCCTTTGTCGTTTTCACTGGAGt < 1:1539840/62‑1 (MQ=255) gaTAACCACCGGACGCAGTGACGGACAAAGTGTTTTAAATCCTTTGTCGTTTTCACTGGAGt < 1:177340/62‑1 (MQ=255) gaTAACCACCGGACGCAGTGACGGACAAAGTGTTTTAAATCCTTTGTCGTTTTCACTGGAGt < 1:2037172/62‑1 (MQ=255) gaTAACCACCGGACGCAGTGACGGACAAAGTGTTTTAAATCCTTTGTCGTTTTCACTGGAGt < 1:257355/62‑1 (MQ=255) aaCCACCGGACGCAGTGACGGACAAAGTGTTTTAAATCCTTTGTCGTTTTCACTGGAGTAAGATTCACg > 1:1962828/1‑69 (MQ=255) aaCCACCGGACGCAGTGACGGACAAAGTGTTTTAAATCCTTTGTCGTTTTCACTGGAGTAAGATTCACg > 1:2624874/1‑69 (MQ=255) aaCCACCGGACGCAGTGACGGACAAAGTGTTTTAAATCCTTTGTCGTTTTCACTGGAGTAAGATTCACg > 1:2890905/1‑69 (MQ=255) aaCCACCGGACGCAGTGACGGACAAAGTGTTTTAAATCCTTTGTCGTTTTCACTGGAGTAAGATTCACg > 1:2941610/1‑69 (MQ=255) accaccGGACGCAGTGACGGACAAAGTGTTTTAAATCCTTTGTCGTTTTCACTGGAGTAAGATTCACGc > 1:1698863/1‑69 (MQ=255) accaccGGACGCAGTGACGGACAAAGTGTTTTAAATCCTTTGTCGTTTTCACTGGAGTAAGATTCACGc > 1:1392471/1‑69 (MQ=255) accaccGGACGCAGTGACGGACAAAGTGTTTTAAATCCTTTGTCGTTTTCACTGGAGTAAGATTCACGc > 1:76180/1‑69 (MQ=255) aaaGTGTTTTAAATCCTTTGTCGTTTTCACTGGAGt < 1:3076226/36‑1 (MQ=255) | TTCTCGAACAGGCGTCCCATCTGACCGCCACCGCCGACGATAACCACCGGACGCAGTGACGGACAAAGTGTTTTAAATCCTTTGTCGTTTTCACTGGAGTAAGATTCACGC > minE/1772409‑1772519 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |