| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,775,329 | G→C | 34.5% | G87G (GGG→GGC) | yfiN → | predicted diguanylate cyclase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,775,329 | 0 | G | C | 34.5% | 50.2 / 41.3 | 55 | G87G (GGG→GGC) | yfiN | predicted diguanylate cyclase |
| Reads supporting (aligned to +/- strand): ref base G (14/22); new base C (12/7); total (26/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.92e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.12e-01 | |||||||||||
GTTTTTGCCGATGGCCCTGCAGCAACTGAAACACTGGCAGCGCTGGGCCAGCAAGGGCAATTTTCAACTGCAGAAGTACGTGATAAGCAGCAAAATATTCTGGCATCCTGGCAT > minE/1775273‑1775386 | gTTTTTGCCGATGGCCCTGCAGCAACTGAAACACTGGCAGCGCTGGGCCAGCAAGGGCAATTTTCAAc > 1:2987969/1‑68 (MQ=255)gTTTTTGCCGATGGCCCTGCAGCAACTGAAACACTGGCAGCGCTGGGCCAGCAAGGGCAATTTTCAAc > 1:1194666/1‑68 (MQ=255)gTTTTTGCCGATGGCCCTGCAGCAACTGAAACACTGGCAGCGCTGGGCCAGCAAGGGCAATTTTCAAc > 1:321426/1‑68 (MQ=255) ttttGCCGATGGCCCTGCAGCAACTGAAACACTGGCAGCGCTGGGCCAGCAAGGCCAATTTTCAACTg < 1:105301/68‑1 (MQ=255) ttttGCCGATGGCCCTGCAGCAACTGAAACACTGGCAGCGCTGGGCCAGCAAGGCCAATTTTCAACTg < 1:3278963/68‑1 (MQ=255) ttttGCCGATGGCCCTGCAGCAACTGAAACACTGGCAGCGCTGGGCCAGCAAGGCCAATTTTCAACTg < 1:3436429/68‑1 (MQ=255) ttttGCCGATGGCCCTGCAGCAACTGAAACACTGGCAGCGCTGGGCCAGCAAGGCCAATTTTCAACTg < 1:2010328/68‑1 (MQ=255) ttttGCCGATGGCCCTGCAGCAACTGAAACACTGGCAGCGCTGGGCCAGCAAGGCCAATTTTCAACTg < 1:1859098/68‑1 (MQ=255) ttttGCCGATGGCCCTGCAGCAACTGAAACACTGGCAGCGCTGGGCCAGCAAGGCCAATTTTCAACTg < 1:538795/68‑1 (MQ=255) gATGGCCCTGCAGCAACTGAAACACTGGCAGCGCTGGGCCAGCAAGGCCAATTTTCAACTg < 1:3013929/61‑1 (MQ=255) aTGGCCCTGCAGCAACTGAAACACTGGCAGCGCTGGGCCAGCAAGGCCAATTTTc > 1:1547193/1‑55 (MQ=255) aTGGCCCTGCAGCAACTGAAACACTGGCAGCGCTGGGCCAGCAAGGCCAATTTTc > 1:1777197/1‑55 (MQ=255) aTGGCCCTGCAGCAACTGAAACACTGGCAGCGCTGGGCCAGCAAGGCCAATTTTc > 1:1469074/1‑55 (MQ=255) aTGGCCCTGCAGCAACTGAAACACTGGCAGCGCTGGGCCAGCAAGGCCAATTTTc > 1:1381972/1‑55 (MQ=255) aTGGCCCTGCAGCAACTGAAACACTGGCAGCGCTGGGCCAGCAAGGCCAATTTTc > 1:1987958/1‑55 (MQ=255) aTGGCCCTGCAGCAACTGAAACACTGGCAGCGCTGGGCCAGCAAGGCCAATTTTc > 1:266698/1‑55 (MQ=255) aTGGCCCTGCAGCAACTGAAACACTGGCAGCGCTGGGCCAGCAAGGCCAATTTTc > 1:2640643/1‑55 (MQ=255) aTGGCCCTGCAGCAACTGAAACACTGGCAGCGCTGGGCCAGCAAGGCCAATTTTc > 1:2068004/1‑55 (MQ=255) aTGGCCCTGCAGCAACTGAAACACTGGCAGCGCTGGGCCAGCAAGGCCAATTTTc > 1:919772/1‑55 (MQ=255) aTGGCCCTGCAGCAACTGAAACACTGGCAGCGCTGGGCCAGCAAGGCCAATTTTc > 1:2524911/1‑55 (MQ=255) aTGGCCCTGCAGCAACTGAAACACTGGCAGCGCTGGGCCAGCAAGGCCAATTTTc > 1:939188/1‑55 (MQ=255) aTGGCCCTGCAGCAACTGAAACACTGGCAGCGCTGGGCCAGCAAGGCCAATTTTc > 1:227791/1‑55 (MQ=255) cagcaACTGAAACACTGGCAGCGCTGGGCCAGCAAGGGCAATTTTCAACTGCAGAAGTACGTGATAagc > 1:2908409/1‑69 (MQ=255) cagcaACTGAAACACTGGCAGCGCTGGGCCAGCAAGGGCAATTTTCAACTGCAGAAGTACGTGATAagc > 1:2970415/1‑69 (MQ=255) cagcaACTGAAACACTGGCAGCGCTGGGCCAGCAAGGGCAATTTTCAACTGCAGAAGTACGTGATAagc > 1:1998739/1‑69 (MQ=255) cagcaACTGAAACACTGGCAGCGCTGGGCCAGCAAGGGCAATTTTCAACTGCAGAAGTACGAGATAagc > 1:1627539/1‑69 (MQ=255) gAAACACTGGCAGCGCTGGGCCAGCAAGGGCAATTTTc < 1:1049358/38‑1 (MQ=255) gAAACACTGGCAGCGCTGGGCCAGCAAGGGCAATTTTc < 1:3175788/38‑1 (MQ=255) gAAACACTGGCAGCGCTGGGCCAGCAAGGGCAATTTTc > 1:2195408/1‑38 (MQ=255) gAAACACTGGCAGCGCTGGGCCAGCAAGGGCAATTTTc < 1:2460662/38‑1 (MQ=255) gAAACACTGGCAGCGCTGGGCCAGCAAGGGCAATTTTc < 1:3405032/38‑1 (MQ=255) gAAACACTGGCAGCGCTGGGCCAGCAAGGGCAATTTTc < 1:680466/38‑1 (MQ=255) gAAACACTGGCAGCGCTGGGCCAGCAAGGGCAATTTTc < 1:1469809/38‑1 (MQ=255) aaaCACTGGCAGCGCTGGGCCAGCAAGGGCAATTTTc < 1:947090/37‑1 (MQ=255) cTGGCAGGGCTGGGCCAGCAAGGGCAATTTTCAACTGCAGAAGTACGTGATAAGCAGCAAAATATTCt > 1:2680125/1‑68 (MQ=255) cTGGCAGCGCTGGGCCAGCAAGGGCAATTTTCAACTGCAGAAGTACGTGATAAGCAGCAAAATATTCt > 1:280866/1‑68 (MQ=255) cTGGCAGCGCTGGGCCAGCAAGGGCAATTTTCAACTGCAGAAGTACGTGATAAGCAGCAAAATATTCt > 1:2686186/1‑68 (MQ=255) cTGGCAGCGCTGGGCCAGCAAGGGCAATTTTCAACTGCAGAAGTACGTGATAAGCAGCAAAATATTCt > 1:1352862/1‑68 (MQ=255) cTGGCAGCGCTGGGCCAGCAAGGGCAATTTTCAACTGCAGAAGTACGTGATAAGCAGCAAAATATTCt > 1:3246049/1‑68 (MQ=255) cTGGCAGCGCTGGGCCAGCAAGGGCAATTTTCAACTGCAGAAGTACGTGATAAGCAGCAAAATATTCt > 1:3429565/1‑68 (MQ=255) cTGGGCCAGCAAGGGCAATTTTCAACTGCAGAAGTACGTGATAAGCAGCAAAATATTCTGGCATCCTgg < 1:1312061/69‑1 (MQ=255) cTGGGCCAGCAAGGGCAATTTTCAACTGCAGAAGTACGTGATAAGCAGCAAAATATTCTGGCATCCTgg < 1:650348/69‑1 (MQ=255) cTGGGCCAGCAAGGGCAATTTTCAACTGCAGAAGTACGTGATAAGCAGCAAAATATTCTGGCATCCTgg < 1:1812286/69‑1 (MQ=255) cTGGGCCAGCAAGGGCAATTTTCAACTGCAGAAGTACGTGATAAGCAGCAAAATATTCTGGCATCCTgg < 1:2073113/69‑1 (MQ=255) cTGGGCCAGCAAGGGCAATTTTCAACTGCAGAAGTACGTGATAAGCAGCAAAATATTCTGGCATCCTgg < 1:2124425/69‑1 (MQ=255) cTGGGCCAGCAAGGGCAATTTTCAACTGCAGAAGTACGTGATAAGCAGCAAAATATTCTGGCATCCTgg < 1:3383991/69‑1 (MQ=255) cTGGGCCAGCAAGGGCAATTTTCAACTGCAGAAGTACGTGATAAGCAGCAAAATATTCTGGCATCCTgg < 1:2224095/69‑1 (MQ=255) cTGGGCCAGCAAGGGCAATTTTCAACTGCAGAAGTACGTGATAAGCAGCAAAATATTCTGGCATCCTgg < 1:2395066/69‑1 (MQ=255) cTGGGCCAGCAAGGGCAATTTTCAACTGCAGAAGTACGTGATAAGCAGCAAAATATTCTGGCATCCTgg < 1:2398817/69‑1 (MQ=255) cTGGGCCAGCAAGGGCAATTTTCAACTGCAGAAGTACGTGATAAGCAGCAAAATATTCTGGCATCCTgg < 1:2430890/69‑1 (MQ=255) tGGGCCAGCAAGGGCAATTTTGAACTGCAGAAGTACGTGATAAGCAGCAAAATATTCTGGCATCCTgg < 1:2206705/68‑1 (MQ=255) tGGGCCAGCAAGGGCAATTTTCAACTGCAGAAGTACGTGATAAGCAGCAAAATATTCTGGCATCCTgg < 1:1379776/68‑1 (MQ=255) ccAGCAAGGGCAATTTTCAACTGCAGAAGTACGTGATAAGCAGCAAAATATTCTGGCATCCTGGCAt < 1:468263/67‑1 (MQ=255) ccAGCAAGGGCAATTTTCAACTGCAGAAGTACGTGATAAGCAGCAAAATATTCTGGCATCCTGGCAt < 1:1320398/67‑1 (MQ=255) ccAGCAAGGGCAATTTTCAACTGCAGAAGTACGTGATAAGCAGCAAAATATTCTGGCATCCTGGCAt < 1:2706474/67‑1 (MQ=255) | GTTTTTGCCGATGGCCCTGCAGCAACTGAAACACTGGCAGCGCTGGGCCAGCAAGGGCAATTTTCAACTGCAGAAGTACGTGATAAGCAGCAAAATATTCTGGCATCCTGGCAT > minE/1775273‑1775386 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |