| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,775,995 | C→A | 36.7% | I309I (ATC→ATA) | yfiN → | predicted diguanylate cyclase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,775,995 | 0 | C | A | 36.7% | 85.5 / 74.4 | 98 | I309I (ATC→ATA) | yfiN | predicted diguanylate cyclase |
| Reads supporting (aligned to +/- strand): ref base C (52/10); new base A (13/23); total (65/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.22e-06 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.27e-01 | |||||||||||
Only 100 of 119 total aligned reads displayed.
AATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACCGCCTGGGCGGCGATGAATTCGC > minE/1775933‑1776063 | aaTTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCaaaa > 1:2280817/1‑69 (MQ=255)aaTTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCaaaa > 1:2466451/1‑69 (MQ=255)aaTTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCaaaa > 1:2984382/1‑69 (MQ=255)aaTTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCaaaa > 1:1332565/1‑69 (MQ=255)aaTTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCaaaa > 1:2964702/1‑69 (MQ=255) aTTTCAAATATATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATAGCaaaa > 1:2458168/1‑68 (MQ=255) aTTTCAAATATATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATAGCAAAAc > 1:2234095/1‑69 (MQ=255) aTTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAAc > 1:2315585/1‑69 (MQ=255) aTTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAAc > 1:332603/1‑69 (MQ=255) aTTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAAc > 1:3274124/1‑69 (MQ=255) aTTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAAc > 1:3101932/1‑69 (MQ=255) ttCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACg > 1:982388/1‑68 (MQ=255) tCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACgtt < 1:3412721/69‑3 (MQ=255) tCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACg > 1:3094738/1‑67 (MQ=255) tCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGt < 1:664067/69‑1 (MQ=255) tCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGt < 1:673211/69‑1 (MQ=255) tCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGt < 1:1035138/69‑1 (MQ=255) tCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTAATTGAAATCGCAAAACGGt < 1:3089035/69‑1 (MQ=255) cAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGt < 1:2920645/68‑1 (MQ=255) aCATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTg > 1:1298158/1‑69 (MQ=255) aCATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTg > 1:2703207/1‑69 (MQ=255) aCATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTg > 1:299643/1‑69 (MQ=255) cATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTT‑GCTGaa > 1:289444/1‑69 (MQ=255) cATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTT‑GCTGaa > 1:1656651/1‑69 (MQ=255) aaTGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAAttt > 1:1004920/1‑69 (MQ=255) aaTGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAAttt > 1:1225562/1‑69 (MQ=255) aaTGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAAttt > 1:443249/1‑69 (MQ=255) aaTGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAAttt > 1:188041/1‑69 (MQ=255) aaTGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAAttt > 1:923551/1‑69 (MQ=255) aTGCGACGGGCGATAGAGTCTTGATTGAAATAGCAAAACGGTTAg < 1:1549440/45‑1 (MQ=255) aTGCGACGGGCGATAGAGTCTTGATTGAAATAGCAAAACGGTTAg < 1:1423167/45‑1 (MQ=255) aTGCGACGGGCGATAGAGTCTTGATTGAAATAGCAAAACGGTTAg < 1:3036837/45‑1 (MQ=255) aTGCGACGGGCGATAGAGTCTTGATTGAAATAGCAAAACGGTTAg < 1:2392416/45‑1 (MQ=255) aTGCGACGGGCGATAGAGTCTTGATTGAAATAGCAAAACGGTTAg < 1:1818417/45‑1 (MQ=255) aTGCGACGGGCGATAGAGTCTTGATTGAAATAGCAAAACGGTTAg < 1:968403/45‑1 (MQ=255) aTGCGACGGGCGATAGAGTCTTGATTGAAATAGCAAAACGGTTAg < 1:2320843/45‑1 (MQ=255) aTGCGACGGGCGATAGAGTCTTGATTGAAATAGCAAAACGGTTAg < 1:167584/45‑1 (MQ=255) aTGCGACGGGCGATAGAGTCTTGATTGAAATAGCAAAACGGTTAg < 1:2312889/45‑1 (MQ=255) aTGCGACGGGCGATAGAGTCTTGATTGAAATAGCAAAACGGTTAg < 1:2275078/45‑1 (MQ=255) aTGCGACGGGCGATAGAGTCTTGATTGAAATAGCAAAACGGTTAg < 1:222591/45‑1 (MQ=255) aTGCGACGGGCGATAGAGTCTTGATTGAAATAGCAAAACGGTTAg < 1:3308781/45‑1 (MQ=255) aTGCGACGGGCGATAGAGTCTTGATTGAAATAGCAAAACGGTTAg < 1:1146982/45‑1 (MQ=255) aTGCGACGGGCGATAGAGTCTTGATTGAAATAGCAAAACGGTTAg < 1:1658740/45‑1 (MQ=255) aTGCGACGGGCGATAGAGTCTTGATTGAAATAGCAAAACGGTTAg < 1:2071276/45‑1 (MQ=255) aTGCGACGGGCGATAGAGTCTTGATTGAAATAGCAAAACGGTTAg < 1:487913/45‑1 (MQ=255) aTGCGACGGGCGATAGAGTCTTGATTGAAATAGCAAAACGGTTAg < 1:1914252/45‑1 (MQ=255) aTGCGACGGGCGATAGAGTCTTGATTGAAATAGCAAAACGGTTAg < 1:595939/45‑1 (MQ=255) aTGCGACGGGCGATAGAGTCTTGATTGAAATAGCAAAACGGTTAg < 1:1113913/45‑1 (MQ=255) aTGCGACGGGCGATAGAGTCTTGATTGAAATAGCAAAACGGTTAGCTGAATTTg > 1:1859847/1‑54 (MQ=255) aTGCGACGGGCGATAGAGTCTTGATTGAAATAGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACa > 1:1456138/1‑67 (MQ=255) aTGCGACGGGCGATAGAGTCTTGATTGAAATAGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACa > 1:1369367/1‑67 (MQ=255) aTGCGACGGGCGATAGAGTCTTGATTGAAATAGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACa > 1:2725222/1‑67 (MQ=255) aTGCGACGGGCGATAGAGTCTTGATTGAAATAGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACa > 1:3186315/1‑67 (MQ=255) aTGCGACGGGCGATAGAGTCTTGATTGAAATAGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACa > 1:2355971/1‑67 (MQ=255) aTGCGACGGGCGATAGAGTCTTGATTGAAATAGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACa > 1:1216189/1‑67 (MQ=255) aTGCGACGGGCGATAGAGTCTTGATTGAAATAGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACa > 1:700617/1‑67 (MQ=255) tGCGACGGGCGATAGAGTCTTGATTGAAATAGCAAAACGGTTAg < 1:1393485/44‑1 (MQ=255) tcGATAGAGTCTTGATTGAAATAGCATAACGGTTAg < 1:186026/35‑1 (MQ=37) agagTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACCGCCTg > 1:1790780/1‑69 (MQ=255) gTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTTCGACATAAAGCATACCGCCTGggc > 1:1618005/1‑69 (MQ=255) gTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACCGCCTggg > 1:2028434/1‑68 (MQ=255) gTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACCGCCTGggc > 1:1568260/1‑69 (MQ=255) gTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACCGCCTGggc > 1:789425/1‑69 (MQ=255) gTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACCGCCTGggc > 1:1884369/1‑69 (MQ=255) gTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACCGCCTGggc > 1:237414/1‑69 (MQ=255) gTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACCGCCTGggc > 1:405903/1‑69 (MQ=255) gTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACCGCCTGggc > 1:285800/1‑69 (MQ=255) gTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACCGCCTGggc > 1:3247603/1‑69 (MQ=255) ttgattgaAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACCGCCTGggcggc > 1:356384/1‑69 (MQ=255) ttgattgaAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACCGCCTGggcggc > 1:1818579/1‑69 (MQ=255) ttgattgaAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACCGCCTGggcggc < 1:426995/69‑1 (MQ=255) ttgattgaAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACCGCCTGggcggc > 1:1456358/1‑69 (MQ=255) ttgattgaAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACCGCCTGggcggc > 1:2567343/1‑69 (MQ=255) ttgattgaAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACCGCCTGggcggc > 1:243225/1‑69 (MQ=255) tgaAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACCGCCTGGGCGGCGATg < 1:532159/68‑1 (MQ=255) tgaAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACCGCCTGGGCGGCGATg > 1:1772879/1‑68 (MQ=255) tgaAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACCGCCTGGGCGGCGATGa > 1:1852733/1‑69 (MQ=255) tgaAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACCGCCTGGGCGGCGATGa > 1:187140/1‑69 (MQ=255) tgaAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACCGCCTGGGCGGCGATGa > 1:1100880/1‑69 (MQ=255) tgaAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACCGCCTGGGCGGCGATGa > 1:2115322/1‑69 (MQ=255) tgaAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACCGCCTGGGCGGCGATGa > 1:1260975/1‑69 (MQ=255) tgaAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACCGCCTGGGCGGCGATGa > 1:212490/1‑69 (MQ=255) tgaAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACCGCCTGGGCGGCGATGa > 1:75618/1‑69 (MQ=255) tgaAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGAATACCGCCTGGGCGGCGATGa > 1:2793641/1‑69 (MQ=255) cGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACCGCCTGGGCGGCGATGAATTCGc < 1:3003397/69‑1 (MQ=255) cGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACCGCCTGGGCGGCGATGAATTCGc < 1:690358/69‑1 (MQ=255) cGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACCGCCTGGGCGGCGATGAATTCGc < 1:714480/69‑1 (MQ=255) cGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACCGCCTGGGCGGCGATGAATTCGc < 1:2812650/69‑1 (MQ=255) cGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACCGCCTGGGCGGCGATGAATTCGc < 1:2943099/69‑1 (MQ=255) cGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACCGCCTGGGCGGCGATGAATTCGc < 1:530509/69‑1 (MQ=255) cGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACCGCCTGGGCGGCGATGAATTCGc < 1:2358725/69‑1 (MQ=255) cGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACCGCCTGGGCGGCGATGAATTCGc < 1:456985/69‑1 (MQ=255) cGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACCGCCTGGGCGGCGATGAATTCGc < 1:414807/69‑1 (MQ=255) cGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACCGCCTGGGCGGCGATGAATTCGc < 1:3101649/69‑1 (MQ=255) cGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACCGCCTGGGCGGCGATGAATTCGc < 1:3379976/69‑1 (MQ=255) cGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACCGCCTGGGCGGCGATGAATTCGc < 1:2199106/69‑1 (MQ=255) cGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACCGCCTGGGCGGCGATGAATTCGc < 1:2203325/69‑1 (MQ=255) cGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACCGCCTGGGCGGCGATGAATTCGc < 1:3283076/69‑1 (MQ=255) cGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACCGCCTGGGCGGCGATGAATTCGc < 1:1250285/69‑1 (MQ=255) cGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACCGCCTGGGCGGCGATGAATTCGc < 1:316735/69‑1 (MQ=255) | AATTTCAAATACATCAATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATCGCAAAACGGTTAGCTGAATTTGGCGGGCTGCGACATAAAGCATACCGCCTGGGCGGCGATGAATTCGC > minE/1775933‑1776063 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |